Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 4,188,484 Δ27 bp 100% coding (3136‑3162/4224 nt) rpoC → RNA polymerase, beta prime subunit

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4188484 4188510 27 61 [0] [0] 61 rpoC RNA polymerase, beta prime subunit

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 41884830 (0.000)58 (1.240) 47/268 NT 100% coding (3135/4224 nt) rpoC RNA polymerase, beta prime subunit
?NC_000913 4188511 = 0 (0.000)coding (3163/4224 nt) rpoC RNA polymerase, beta prime subunit

AGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCC  >  NC_000913/4188363‑4188653
                                                                                                                                                    |                                                                                                                                              
agttccttacggtgcggtactggcgaaaggcgatggcgaacaggttgctggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccg                                                                                                                                                <  1:365961‑M2/1‑1 (MQ=255)
     cttacggtgcggtactggcgaaaggcgatggcgaacaggttgctggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGgtctg                                                                                                                                           <  1:664538‑M2/6‑1 (MQ=255)
              cggtactggcgaaaggcgatggcgaacaggttgctggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGCCTGTCTTCGCtg                                                                                                                                  >  2:400624‑M2/135‑149 (MQ=255)
                 tactggcgaaaggcgatggcgaacaggttgctggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCtggtg                                                                                                                               >  1:45423‑M2/132‑149 (MQ=255)
                   ctggcgaaaggcgatggcgaacaggttgcgggcggcgaaaccgttgcaaactgggacccgcacaccaggccgggtatcaacgaagtaggcggggttgtacgctttactgacaagatcgacggccagagcGGCCTGTATTCGccggtggg                                                                                                                             >  2:275734‑M2/130‑148 (MQ=255)
                     ggcgaaaggcgatggcgaacaggttgctggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGTTGGTTc                                                                                                                           >  2:393314‑M2/128‑149 (MQ=255)
                      gcgaaaggcgatggcgaacaggttgctggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCt                                                                                                                          >  1:147639‑M2/127‑149 (MQ=255)
                      gcgaaaggcgatggcgaacaggttgctggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCt                                                                                                                          <  1:513303‑M2/23‑1 (MQ=255)
                      gcgaaaggcgatggcgaacaggttgctggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCCTCGCTGGTGGTTCt                                                                                                                          >  2:822798‑M2/127‑149 (MQ=255)
                       cgaaaggcgatggcgaacaggttgctggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttacggacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTg                                                                                                                         <  1:358938‑M2/24‑1 (MQ=255)
                            ggccatggctaaccggttgctggcggcgaaaccgttgcaaactggtacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgccttactgacatgatcgacggccagaccGGTCTGTCCTCGCTGTTGGTTCTGGATTc                                                                                                                    <  2:688494‑M2/29‑1 (MQ=255)
                               gatggcgaacaggttgctggcggtgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtaccctttactgacatgatcgaccccccgaacGGCCTGTCATCGCTGGTCATTCTGGAATCcga                                                                                                                 <  2:539238‑M2/32‑2 (MQ=255)
                               gatggcgaacaggttgctggcggcgaaaccgttacaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGc                                                                                                                 <  2:681183‑M2/32‑1 (MQ=255)
                                atggcgaacaggttgctggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCa                                                                                                                <  1:790416‑M2/33‑1 (MQ=255)
                                        caggttgctggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTAc                                                                                                        <  2:450509‑M2/41‑1 (MQ=255)
                                        cagggtgctggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTAc                                                                                                        <  2:205893‑M2/41‑1 (MQ=255)
                                             tgctggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAg                                                                                                   >  1:375238‑M2/104‑149 (MQ=255)
                                                tggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatctctgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAggtg                                                                                                >  1:819360‑M2/101‑149 (MQ=255)
                                                tggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTCCTGGATTCCGCAGAACGTACGGcaggcg                                                                                                >  2:513902‑M2/101‑147 (MQ=255)
                                                tggcggcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGGCTGTCTTCGCTGTTGGTGCTGGATTCCGCAGAACGTACCGCAgatg                                                                                                >  1:398831‑M2/101‑149 (MQ=255)
                                                     gcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTaaa                                                                                           <  2:385576‑M2/54‑1 (MQ=255)
                                                     gcgaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTaaa                                                                                           >  2:594617‑M2/96‑149 (MQ=255)
                                                      cgaaaccgttgcaaactgggacccgcacaccataccggttatcaccgaagtaagcggttttgtacgatttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAg                                                                                          >  1:177700‑M2/95‑149 (MQ=255)
                                                      cgaaaccggtgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAg                                                                                          <  2:448598‑M2/55‑1 (MQ=255)
                                                       gaaaccgttgtaaactgtgacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGGCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAAGTGGTAAagg                                                                                         >  2:329101‑M2/94‑148 (MQ=255)
                                                       gaaaccgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTGCCGCAGGTGGTAAAGa                                                                                         >  2:174576‑M2/94‑149 (MQ=255)
                                                          accgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCGGAAGAATGTACCGCAGGTGGTAAAGATCt                                                                                      >  2:600022‑M2/91‑149 (MQ=255)
                                                            cgttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGc                                                                                    <  2:841265‑M2/61‑1 (MQ=255)
                                                              ttgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGt                                                                                  >  2:56861‑M2/87‑149 (MQ=255)
                                                               tgcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTc                                                                                 >  1:290703‑M2/86‑149 (MQ=255)
                                                                gcaaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgatttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGCTTCCGCAGAACGTACCGCAGGGGGTAAAGATCTGCGTcc                                                                                >  2:27505‑M2/85‑149 (MQ=255)
                                                                  aaactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGGCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCgg                                                                              <  1:246164‑M2/67‑1 (MQ=255)
                                                                   aactgggacccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGc                                                                             <  2:86700‑M2/68‑1 (MQ=255)
                                                                        ggacccgcacaccctgccggttatccccgaagtaagcggttttgtacgctttactggcatgaccgccggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGa                                                                        <  2:330371‑M2/73‑1 (MQ=255)
                                                                          acccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGaaa                                                                      >  1:58025‑M2/75‑149 (MQ=255)
                                                                          acccgcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCGGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGaaa                                                                      <  1:172788‑M2/75‑1 (MQ=255)
                                                                              gcacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCg                                                                  <  1:691156‑M2/79‑1 (MQ=255)
                                                                               cacaccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGt                                                                 <  2:574073‑M2/80‑1 (MQ=255)
                                                                                 caccatgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTg                                                               <  2:425069‑M2/82‑1 (MQ=255)
                                                                                    catgccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATg                                                            >  1:31073‑M2/65‑149 (MQ=255)
                                                                                        ccggttatcaccgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCa                                                        <  2:218009‑M2/89‑1 (MQ=255)
                                                                                                 accgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAgggtacaga                                               >  2:300524‑M2/52‑145 (MQ=255)
                                                                                                 accgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                               >  2:197364‑M2/52‑149 (MQ=255)
                                                                                                 accgaagtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                               >  2:631685‑M2/52‑149 (MQ=255)
                                                                                                   cggaggaagcggttttggacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacgacg                                             <  1:15347‑M2/100‑1 (MQ=255)
                                                                                                      agtaagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTc                                          <  1:74275‑M2/103‑1 (MQ=255)
                                                                                                       gcgagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCt                                         <  2:853942‑M2/104‑1 (MQ=255)
                                                                                                         aagcggttttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGa                                       <  2:535201‑M2/106‑1 (MQ=255)
                                                                                                                tttgtacgctttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAgg                                >  1:17881‑M2/37‑149 (MQ=255)
                                                                                                                      cgctttactgacatgctcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATTTTCACGGTAACGACGTTCTGATCCCAGGTACCGa                          <  1:631685‑M2/119‑1 (MQ=255)
                                                                                                                         tttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGTTTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGatat                       >  1:806176‑M2/28‑149 (MQ=255)
                                                                                                                         tttactgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGatat                       <  2:659002‑M2/122‑1 (MQ=255)
                                                                                                                           tactgacatgatcgacggccagaccTGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                     <  1:528225‑M2/124‑1 (MQ=255)
                                                                                                                             ccgacatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCa                   <  1:56861‑M2/126‑1 (MQ=255)
                                                                                                                                acatgatcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcg                >  1:630547‑M2/21‑149 (MQ=255)
                                                                                                                                acatgatcgacggccagaccGGTCTGTCTTAGCTCACAGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcg                >  1:847774‑M2/21‑149 (MQ=255)
                                                                                                                                     atcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGGTATGCCAGCGCAGTa           >  1:12917‑M2/16‑149 (MQ=255)
                                                                                                                                     atcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTa           >  1:648920‑M2/16‑149 (MQ=255)
                                                                                                                                     atcgacggccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTa           >  2:277457‑M2/16‑149 (MQ=255)
                                                                                                                                             ccagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGc   >  2:378978‑M2/8‑149 (MQ=255)
                                                                                                                                              cagaccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGcc  >  1:356173‑M2/7‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
AGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCC  >  NC_000913/4188363‑4188653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.