Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 844,358 C→T G375S (GGT→AGT)  ybiO ← mechanosensitive channel protein, intermediate conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913844,3580CT96.2% 220.6 / NA 83G375S (GGT→AGT) ybiOmechanosensitive channel protein, intermediate conductance
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (40/37);  total (44/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAG  >  NC_000913/844213‑844505
                                                                                                                                                 |                                                                                                                                                   
agccagccATTCAACCGTGTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTcat                                                                                                                                                  <  1:1212727/149‑1 (MQ=255)
  ccagccaTTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCCGGCTGCGCACCGTTGCACtcatca                                                                                                                                                >  2:183066/1‑149 (MQ=255)
      ccaTTCAACCGTTTTTGCAGCTCCGGCTCGTTGTTCAGTGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATaaa                                                                                                                                            >  2:303121/1‑149 (MQ=255)
      ccaTTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATaaa                                                                                                                                            >  1:1273300/1‑149 (MQ=255)
        aTTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAGAACATACGGGAAACAAACGCTGCGGTACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAACtt                                                                                                                                          >  2:1262951/1‑149 (MQ=255)
        aTTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACAGTTGCACTCATCATAAAtt                                                                                                                                          >  2:626821/1‑149 (MQ=255)
         ttCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAAttt                                                                                                                                         >  1:452611/1‑149 (MQ=255)
            aaCCGTTTTTGCAGCTCCGGATAGTAACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAg                                                                                                                                      >  1:834183/1‑149 (MQ=255)
             aCCGTTTTTGCGGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACATACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAgg                                                                                                                                     >  2:1000434/1‑149 (MQ=255)
               cGTTTTTGCAGCTCCGGATAGTTACGCTGGGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCt                                                                                                                                   <  2:379474/149‑1 (MQ=255)
                gTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCtt                                                                                                                                  >  1:500433/1‑148 (MQ=255)
                     tGCAGCTCCGGATAGTTACGCTGAGGAGGGGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAATCAATCGCTGTGATACCAATTATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGcc                                                                                                                             <  1:826150/149‑1 (MQ=255)
                      gCAGCTCCTGAGTGTTACGCTGAGTATGGGGCGGGAGTGGGATGGGTTTCGCCAGCCAGCGGGAACACATACCGGAAACAAACGCTGCGATCCCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCCGTCGccc                                                                                                                            <  2:1095213/149‑1 (MQ=255)
                      gCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGccc                                                                                                                            >  1:1176611/1‑149 (MQ=255)
                       cAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCg                                                                                                                           <  2:362968/149‑1 (MQ=255)
                       cAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCg                                                                                                                           <  2:1354350/149‑1 (MQ=255)
                        aGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCgg                                                                                                                          >  2:142941/1‑149 (MQ=255)
                          cTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGAt                                                                                                                        >  1:1257933/1‑149 (MQ=255)
                          cTCCGGATAGTTACGCTGAGTAGGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACTAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGAt                                                                                                                        <  2:34569/149‑1 (MQ=255)
                              gtaTAGTTACGGTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAATCATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGaa                                                                                                                    <  2:1010370/147‑1 (MQ=255)
                              ggATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGaa                                                                                                                    <  2:1025253/149‑1 (MQ=255)
                                 tggTTACGCGGAGTATGTGGGGAGAGAGTGATGCTTTTCGCCAGCCAGCGGGAAAACATGCCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGaacaa                                                                                                                 <  2:249943/147‑1 (MQ=255)
                                 tAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATACTCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGaacaa                                                                                                                 <  1:827094/149‑1 (MQ=255)
                                 tAGTTACGCTGAGTACGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGaacaa                                                                                                                 <  2:1033368/149‑1 (MQ=255)
                                  aGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGaacaac                                                                                                                <  1:931033/149‑1 (MQ=255)
                                    ttACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTCTTGCCCGGATCGAACAACga                                                                                                              >  2:484500/1‑149 (MQ=255)
                                     tACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACgaa                                                                                                             <  1:597920/149‑1 (MQ=255)
                                      cggctaggTATGTGGCGAGATACGGATGGTTTCTCCCAGCCAGCTGGAAAACAGTCCCTAAACAAACGCTGCTATACCAATAATCGCCAGCCTGCGCACCGTGGCACACATCATAAATTTCAGGCTGTTGCCCGTATCGAACAACgaaa                                                                                                            <  2:40550/142‑1 (MQ=255)
                                      aCGCTGAGTATGTGGCGGGGGAGGGATGGCTTTCGCCAGCCAGCGGGAAAACATACCGGAAACCAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACgaaa                                                                                                            <  1:841122/149‑1 (MQ=255)
                                       cGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACgaaaa                                                                                                           >  1:1130259/1‑149 (MQ=255)
                                        gCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGAGACCAATAATCGCCAGGCTGCGCACCGGTGCACCCATCATAAATTGCAGGCTGTTGCCCGTATCGAACAACgaaaag                                                                                                          >  1:981273/1‑149 (MQ=255)
                                          tGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACgaaaagaa                                                                                                        >  1:1339338/1‑149 (MQ=255)
                                          ggaggtagtgGCGAGGGAGTGTTGGGTCTCGCCGGCCTGCGCGAAGTCATACCGGAAACAAACGCTGCGATACCAAGAATCCCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCATGCTGTTGCCCGGAGCGAACAACgaaaagaa                                                                                                        <  2:961657/142‑1 (MQ=255)
                                             gTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGaaaaa                                                                                                     <  1:303121/149‑1 (MQ=255)
                                              tATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAAc                                                                                                    >  2:411762/1‑149 (MQ=255)
                                                tgtgGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACAAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATGTCAGGCTGTTGCCTGGATCGAACAAGGAAAAGAAAAACAg                                                                                                  >  1:228731/1‑149 (MQ=255)
                                                  ttgCGAGAGCGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCATCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCa                                                                                                <  1:551197/147‑1 (MQ=255)
                                                  tgGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCa                                                                                                >  2:1159772/1‑149 (MQ=255)
                                                             tGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAAGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAGATaa                                                                                     >  2:1015761/1‑148 (MQ=255)
                                                             tGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATAGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAg                                                                                     >  1:954948/1‑149 (MQ=255)
                                                              gATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGGTCGAACAACGAAAAGAAAAACAGCACGATAAAGTAgg                                                                                    >  2:557807/1‑149 (MQ=255)
                                                               aTGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACCAAAAGAAAAACACCACGAAAAAATAGgc                                                                                   >  2:625855/1‑149 (MQ=255)
                                                                tGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGGTGCACGCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGGTAAAATAGgcg                                                                                  >  1:1351927/1‑149 (MQ=255)
                                                                   ttttCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCtt                                                                               >  1:359058/1‑149 (MQ=255)
                                                                       cgcccgccGGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTgcca                                                                           <  1:1272535/149‑1 (MQ=255)
                                                                       cGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCGCCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTgcca                                                                           >  1:94690/1‑149 (MQ=255)
                                                                        gccagccaGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTgccag                                                                          >  1:886112/1‑149 (MQ=255)
                                                                         ccagccagCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTgccagc                                                                         >  1:1129376/1‑149 (MQ=255)
                                                                          cagccagcGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCCGGCTGCGCACCGTTGCACTCATCATAAATTTCGGGCTGTTGCCCGGCTCGAACAACGAAAAGAAAAACGGCACGAGGAAATAGGCGCGTgccagcc                                                                        >  1:239953/1‑149 (MQ=255)
                                                                           agccagcGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTgccagcca                                                                       <  1:831070/149‑1 (MQ=255)
                                                                            gccagcGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGccagccag                                                                      <  1:689346/149‑1 (MQ=255)
                                                                             ccagcGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGt                                                                     <  1:1390460/149‑1 (MQ=255)
                                                                               agcGGCAAAACATACCGGAAACAAACGCTGCGATACCAATCATCGCCAGCTTGCGCACCGTTGCACTCATCATAAATTTCAGGATGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGc                                                                   <  1:45031/149‑1 (MQ=255)
                                                                                gcGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGCAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGcc                                                                  >  2:1206300/1‑149 (MQ=255)
                                                                                 cGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTTCACTCATCATAAATTTCAGGCTGTTGCTCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCca                                                                 <  2:1019732/149‑1 (MQ=255)
                                                                                 cGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGGCAGGGCCTAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCca                                                                 >  1:669777/1‑149 (MQ=255)
                                                                                  gggAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGAGCGAACAACGAAACGAAAAACAGCACGATAAAATAAGCGCTTGCCAGCCAGGGCcac                                                                >  2:1098813/1‑149 (MQ=255)
                                                                                   ggAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAACTTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCcacc                                                               >  2:24151/1‑148 (MQ=255)
                                                                                    gAAACCATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCacac                                                              <  1:405418/149‑1 (MQ=255)
                                                                                    gAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCAacac                                                              >  2:376799/1‑149 (MQ=255)
                                                                                             ccGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCaaa                                                     <  2:1384757/149‑1 (MQ=255)
                                                                                              cGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCCGCGCAAAg                                                    >  2:1259681/1‑149 (MQ=255)
                                                                                              cGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCAGCAGAAATTTCAGGCTGTGGGCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAg                                                    >  1:194027/1‑149 (MQ=255)
                                                                                              cGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACGCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACCGAAAGATCAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAg                                                    >  1:1299634/1‑149 (MQ=255)
                                                                                                gAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCCGCCAGAGCCACACCAGCCAAAAgcc                                                  >  2:708407/1‑147 (MQ=255)
                                                                                                gAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGgc                                                  >  2:764536/1‑149 (MQ=255)
                                                                                                  accaaacGCTGCGATACCAATAATCGCCGGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAACATAGGCGCTTGCCAGCCAGTGCCACACCAGCGTAAAGgcgc                                                <  2:1273300/147‑1 (MQ=255)
                                                                                                  aacaacgGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCTCTCATCATAAATTTCAGGCTTTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCATTGCCACACCAGCGCAAAGgtgc                                                <  2:452611/149‑1 (MQ=255)
                                                                                                  aacaaacGCGGCGATAGCAATAGTCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAAAAACAAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGgcgc                                                <  2:35500/149‑1 (MQ=255)
                                                                                                   acaaacGCTGCGATACCAATAATCGCCAGGCTCCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCCGCGCAAAGGcgcg                                               <  1:950467/149‑1 (MQ=255)
                                                                                                     aaacGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGa                                             >  1:1129711/1‑149 (MQ=255)
                                                                                                            gcaaTACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAg                                      <  1:367664/146‑1 (MQ=255)
                                                                                                            gCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAACGGCGCGGATAAACAg                                      >  1:870689/1‑149 (MQ=255)
                                                                                                             cGATACCAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAgg                                     <  2:1176611/149‑1 (MQ=255)
                                                                                                               aTACCAATACTGGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAACTAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCt                                   <  2:208337/149‑1 (MQ=255)
                                                                                                                   cAATAATCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGaaa                               >  1:545290/1‑149 (MQ=255)
                                                                                                                      taatCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAg                            <  2:1365520/149‑1 (MQ=255)
                                                                                                                      taatCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAg                            <  1:658321/149‑1 (MQ=255)
                                                                                                                      taatCGCCAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAg                            <  1:484500/149‑1 (MQ=255)
                                                                                                                      taatCGCAAGGCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGAAAGGTACGGGTTACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCAGATAAACAGGCTGAAAAAg                            <  1:1015761/149‑1 (MQ=255)
                                                                                                                          ccccAGGCTGCGCACCGTTGCACTCATCATAAACTTCAGGCTGTTGACCGGATCGAACAACAAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCa                        <  2:1220662/147‑1 (MQ=255)
                                                                                                                                gCTGCGCACCGTTGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAAt                  >  2:1025904/1‑149 (MQ=255)
                                                                                                                                           ttGCACTCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAAAGCCCcgcagag       >  2:1065005/1‑145 (MQ=255)
                                                                                                                                               aCCCATCCTGAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCGCGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGGTg  >  2:366752/1‑148 (MQ=255)
                                                                                                                                                cccATTAAAAATTTCCGGCGGTTACCCGGATCGCACAACGATAATAAAAACATGCCCATAATATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAg  <  2:494984/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAG  >  NC_000913/844213‑844505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: