| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 126,947 | G→T | R418L (CGT→CTT) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 126,947 | 0 | G | T | 98.7% | 256.6 / NA | 79 | R418L (CGT→CTT) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base T (34/43); minor base C (1/0); total (36/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAA > NC_000913/126807‑127088 | tGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTggg < 1:1006476/149‑1 (MQ=255) gCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGt > 2:878863/1‑149 (MQ=255) agcagcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCACGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAAACTCTGGTGCGAACCAGAGCCCTAACTGGGTAAtaa > 2:665619/1‑147 (MQ=255) gcagcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCGTCCAGAAATTCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGAt > 2:1332130/1‑149 (MQ=255) gcagcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTCGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAAGGAt > 1:1263544/1‑149 (MQ=255) cagcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATc < 1:1078146/149‑1 (MQ=255) agcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATcc < 1:277073/149‑1 (MQ=255) gcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAAGCTGAGCCTTAACTGGGTAATGATccc > 1:1114646/1‑149 (MQ=255) cTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCg < 2:506730/149‑1 (MQ=255) cccGGCAGGGACTGGCGGGGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGc < 1:430048/148‑1 (MQ=255) ccaCTGGCGGTTGTATCCCTTGCATTCTGCCTTGGCCGAAGGTGGACTGCAGCAAGTTTTGTGAAATCGAATAATTGGAGCTGGGCCGCATCCATAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACt < 1:633956/147‑1 (MQ=255) cGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATGCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACt > 2:919606/1‑149 (MQ=255) cGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACt < 1:311463/149‑1 (MQ=255) gACTGGCGGTGGTATGCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACta > 2:1205442/1‑148 (MQ=255) gACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATTATCCCGCATGTTACTc < 1:880115/149‑1 (MQ=255) gACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGCTCCCGCATGTTACTc > 1:550624/1‑149 (MQ=255) cTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCAc > 2:1283465/1‑149 (MQ=255) tGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCCCt > 1:44498/1‑149 (MQ=255) tGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACt > 1:1197507/1‑149 (MQ=255) gCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTc > 1:1329073/1‑149 (MQ=255) gCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTc > 1:600681/1‑149 (MQ=255) gCGGTGGTATCCATGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTc < 2:1006736/149‑1 (MQ=255) ggtggtATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGa < 1:149978/149‑1 (MQ=255) ggtggtATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGa < 2:561351/149‑1 (MQ=255) ggtATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACaa > 1:427300/1‑149 (MQ=255) tCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAAcc < 1:973108/149‑1 (MQ=255) cccTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACACAAcca > 2:407596/1‑148 (MQ=255) ccTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTCCTCACTTCGACAAAACCGa > 1:1343985/1‑149 (MQ=255) ctcgCATGCGGCCGTGGCCGAAGGTGGACTTCAGCAAGTATGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTACTTATCCCGCATGTTACTCACTTCGACAAAACCGat < 2:867908/146‑1 (MQ=255) tGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGata > 1:714612/1‑149 (MQ=255) gCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAACACCGATATc > 1:603347/1‑149 (MQ=255) gctgcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCg < 1:665619/149‑1 (MQ=255) tgcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAg > 2:320752/1‑149 (MQ=255) tgcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAAAGGTCTCGCATGTTAGTCACTTCGACAAAACAGGTATCCCCGAg > 2:108028/1‑149 (MQ=255) ccGTGGCCGACGCTGGACTTCAGCAAGAATGCGTCATCCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGCCAAAACCGATATCACCGAGtt > 2:303844/1‑149 (MQ=255) ggggCCGAAGGTGGACTTAAGCAAGGTTGGTGAACTCGAAGAAGTGGAACTGGGCCGCATCCAGAAAACCTCTGGTGCGAACCGGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTgg < 1:1045494/147‑1 (MQ=255) tGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGa < 1:673705/149‑1 (MQ=255) tGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAAATGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGGGCCTTAACTGGGTAATGATCCCGCATGTTACTCACCTCGCCCCAACCGATCTCACCGAGTGGGa > 2:1355754/1‑149 (MQ=255) ggCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGaa < 2:61218/149‑1 (MQ=255) ggCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGaa < 1:1086660/149‑1 (MQ=255) gCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAg < 2:138469/149‑1 (MQ=255) ccGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGc < 2:872325/149‑1 (MQ=255) cGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCg > 2:375039/1‑149 (MQ=255) cGAAGGTGGACTTCAGCAAGTTCGCCGACCCCGCCGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTCACTCACTCCGCCCACACCGACACCACCGAGTTGGAACCg > 2:992543/1‑149 (MQ=255) gAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGCGTTgaggaacg > 2:377161/1‑142 (MQ=255) aaGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGtt < 1:120599/149‑1 (MQ=255) aGGTGGAGTTCATCAAGTTCGTTTAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGCGTAATGATCCCTCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTc < 2:1009982/149‑1 (MQ=255) ggTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTcc < 2:833851/149‑1 (MQ=255) tGGACTTCAGCAAGTTTGGTGAAGTCGAAGGTGTGGAACCGTGTCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATACCACCGAGTTGGAAGCGTTCCGt < 1:33607/149‑1 (MQ=255) aCTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTaaa < 1:1229645/149‑1 (MQ=255) tcagtcaGTTGGGTGAAATGGAAGACGTGGAGCTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGCTCCCGCATGTCACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAAcag < 2:617425/143‑1 (MQ=255) tCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAAcag < 1:1205442/149‑1 (MQ=255) cAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAAcagc < 2:5858/149‑1 (MQ=255) gtcAAGTTTTGTGGAAACGGAGAAGTGGAACTGGGCCGCATCCAGAAAATCCCTGGTGCGAACCTTAGCCTTAACTGAGTAATGATCCCTCATTGTACCCACTTTGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAAcagca < 2:653047/147‑1 (MQ=255) aaGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGaa < 2:1092174/149‑1 (MQ=255) gTTTGGTTAAATCGCAGACTTGGATCGGGGCCGCATACAGGAAACATCTGGTGCGATCTTGAGCCTTAAGTGGGTGATGGTCCCTCGTGTTACTCAGTGCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACg < 2:123106/149‑1 (MQ=255) gTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACgaagaa < 2:746373/149‑1 (MQ=255) aaaTCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGGACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGCGTTGGAACCGTTCCGTAAACAGCAGAACGAAGCAgcg > 2:58162/1‑149 (MQ=255) aaTCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAgcgg < 1:878863/149‑1 (MQ=255) aagaagTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTCACTGGGGAATGATCCCGCACGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACCGCAGAACGAAGAAGAGGCGaaa > 2:148119/1‑149 (MQ=255) gTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCATTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTaa > 1:1237385/1‑149 (MQ=255) gcaCTGGGCCGCATCCAGAAAACCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTCCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCt < 1:1332130/147‑1 (MQ=255) gAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACACAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCt > 2:25850/1‑149 (MQ=255) ccGCATCCAGAACATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACACCAGAACGCAAAAGCGGCGAAACGTAAGCTGGATGGGa > 2:1054431/1‑149 (MQ=255) ccGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGa < 1:1283465/149‑1 (MQ=255) aTCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGAt < 2:427300/149‑1 (MQ=255) gAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGtt > 1:1293208/1‑84 (MQ=255) gAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGtt < 2:1293208/84‑1 (MQ=255) aaaaTCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCAcccc > 1:277674/1‑149 (MQ=255) aaaTCTCTGGGGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCg < 1:159089/149‑1 (MQ=255) aTCTCTGGTGCGAACCTGAGCCTTAACTGGGGAATTATCCCGCATGTTCCTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGt < 1:320752/149‑1 (MQ=255) tGGCGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACGTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCt < 1:1355754/149‑1 (MQ=255) tGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTtca < 2:166995/149‑1 (MQ=255) gCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTtcat < 2:1263544/149‑1 (MQ=255) gCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGCTGGAAGCGTTCCGTAGACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACGCCGGTTGTCTtcat > 1:1051006/1‑149 (MQ=255) cgcaCCTGACCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCATCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTtcatc < 2:1010771/146‑1 (MQ=255) cGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGTAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGCGAACATCACCCCGGTTGCCTtcatc > 2:537995/1‑149 (MQ=255) cTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATgga > 1:897979/1‑147 (MQ=255) cTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGaa > 2:667766/1‑149 (MQ=255) | TGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAA > NC_000913/126807‑127088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |