Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,399,892 A→C 36.2% I214L (ATT→CTT)  miaA → delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,399,8920AC36.2% 24.1 / 41.8 44I214L (ATT→CTT) miaAdelta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase
Reads supporting (aligned to +/- strand):  ref base A (11/17);  new base C (6/10);  total (17/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.06e-01

CCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGC  >  NC_000913/4399745‑4400032
                                                                                                                                                   |                                                                                                                                             
ccACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCAt                                                                                                                                              >  1:317605/1‑149 (MQ=255)
    aaaGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGag                                                                                                                                          >  1:722768/1‑149 (MQ=255)
    aaaGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGag                                                                                                                                          >  1:65210/1‑149 (MQ=255)
        gCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGagcagc                                                                                                                                      <  2:601612/149‑1 (MQ=255)
        gCTTTCCCGGGCACCGCAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGagcagc                                                                                                                                      <  2:712600/149‑1 (MQ=255)
            tCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCCCCATCAACGCCTTGAGCAGCgctc                                                                                                                                  >  1:209086/1‑146 (MQ=255)
              ccGGGCACTGGAAGTTTTTTTCATTTCGGGTAAGACTTTAACGGAACAGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCATGAACTGCTCCATCAACGCCTTGAGCAGCGCTTtc                                                                                                                                >  1:505493/1‑149 (MQ=255)
               cGGGCACTGGAAGTTTTTTTTTTTTTTTTTAAAACATTAACGGCACTGACGCAACCGTCAGGAGACGCGCTACCGTAGCGGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGTACTGTTCCTTCAACGCATTGAGCAGAGGTTtca                                                                                                                               >  2:69658/1‑149 (MQ=255)
                 ggCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTtcatc                                                                                                                             <  1:203499/149‑1 (MQ=255)
                  gCATTGGAACTTCTTTCCTCTTGGGGTAAAACTTGAACGGAACCGACGCAAACGTCAGGAGACGTGCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTtcatca                                                                                                                            <  1:148556/149‑1 (MQ=255)
                  gCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTtcatca                                                                                                                            <  1:330701/149‑1 (MQ=255)
                             ttttttCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑Ctt                                                                                                                <  2:45860/149‑1 (MQ=255)
                              tttttCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACGGCTCCATCAACGCCTTGAGCAGCGTTTTCATCAGATGTTGG‑CTTc                                                                                                               <  1:518229/149‑1 (MQ=255)
                                       tCGGTTAAAAGTTTAACGGAACTTACGCAAACGTAAGTAGATGCTCTACGGTATCAGGTGCATCAGTTCGCCAGCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGa                                                                                                      <  1:213690/149‑1 (MQ=255)
                                       tCGGGTAAAACTTTAACGGAACGGACGCAAACGTCAGGAGACGCTCTACCGGATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGa                                                                                                      <  2:465046/149‑1 (MQ=255)
                                         gggTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAg                                                                                                    <  2:99794/149‑1 (MQ=255)
                                              aaaCTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGaa                                                                                               <  1:350943/149‑1 (MQ=255)
                                                            cTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCtttt                                                                                 <  2:84365/149‑1 (MQ=255)
                                                             tGACGCAAACGTCAGGAGACGCTCTCCCGTGTCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTAAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCttttt                                                                                >  1:406177/1‑149 (MQ=255)
                                                             tGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCttttt                                                                                >  1:667746/1‑149 (MQ=255)
                                                                          aGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGAtt                                                                   <  1:83043/149‑1 (MQ=255)
                                                                                   tctACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACgg                                                          >  1:143830/1‑149 (MQ=255)
                                                                                   tctACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTGGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACgg                                                          >  1:551061/1‑149 (MQ=255)
                                                                                          tATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGACATTTTAGTTT‑AAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGcc                                                   >  1:182568/1‑149 (MQ=255)
                                                                                          tATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGcc                                                   >  1:678731/1‑149 (MQ=255)
                                                                                          tATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGATTTCATCAGATGTTGG‑CTTCAGGGTTTGAAGCAAAAGTCCGGGCGCTTTTTGCCCGAGGAGACTTGCATACGGACTTGcc                                                   >  1:74176/1‑149 (MQ=255)
                                                                                              aGGTGCATCAGTTCGCCAGCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGTCCGAGGAGATTTGTATACGGACTTGCCTTcc                                               <  2:572481/149‑1 (MQ=255)
                                                                                               ggTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCa                                              <  2:722768/149‑1 (MQ=255)
                                                                                               ggTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCa                                              <  2:65210/149‑1 (MQ=255)
                                                                                                     gcAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGtt                                        <  2:255677/148‑1 (MQ=255)
                                                                                                         ttCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGt                                    <  2:503058/149‑1 (MQ=255)
                                                                                                         tccGCCACCGCCCCGGCGGGCCGTGAACTGCGCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGt                                    <  2:729355/147‑1 (MQ=255)
                                                                                                            gCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTggg                                 <  2:649570/149‑1 (MQ=255)
                                                                                                             ccATCGCCCCGGCGAGCCGTGAACTGCTCCATCCACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGAACTTGCCTTCCATTCGTTGCGTGGGt                                >  1:430457/1‑149 (MQ=255)
                                                                                                                     ccGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCa                        <  2:575247/149‑1 (MQ=255)
                                                                                                                         cGAGCCGTGAACCGCTCCATCAACGCCTTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGAtg                    <  1:355837/149‑1 (MQ=255)
                                                                                                                          gAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGAtgt                   >  1:24762/1‑149 (MQ=255)
                                                                                                                            gCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTgg                 <  1:623596/149‑1 (MQ=255)
                                                                                                                             ccGTGAACTGCTCCATCAGCGCAGTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGt                <  1:338781/149‑1 (MQ=255)
                                                                                                                             ccGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGt                <  1:763640/149‑1 (MQ=255)
                                                                                                                                     tGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGAGGTGGTCTTACCtt        >  1:222323/1‑149 (MQ=255)
                                                                                                                                        tCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGTCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGaa     <  1:359675/149‑1 (MQ=255)
                                                                                                                                        tCCATCAACGCATGGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGaa     <  1:582598/149‑1 (MQ=255)
                                                                                                                                           aTCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGc  >  1:124806/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGG‑CTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGC  >  NC_000913/4399745‑4400032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑