Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,235,476 G→A 6.3% intergenic (+70/‑429) pgi → / → yjbE glucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,235,4760GA 6.3% 176.1 / 2.9 64intergenic (+70/‑429)pgi/yjbEglucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base G (26/34);  new base A (2/2);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01

GATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATT  >  NC_000913/4235332‑4235623
                                                                                                                                                |                                                                                                                                                   
gatgatAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgc                                                                                                                                                 <  1:674919/149‑1 (MQ=255)
  tgatAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                                                                                                               <  1:146899/149‑1 (MQ=255)
     tAAAGACATCAGCGGCCACGATAGCTCGACCAATGGTATGATTAACCGCTATAAAGCGTGGCGCGGTTAATCGTCGGCGCTGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCtt                                                                                                                                            <  2:126767/149‑1 (MQ=37)
       aaGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTGACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTGTTAt                                                                                                                                          >  2:44767/1‑149 (MQ=255)
              cagcagCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcat                                                                                                                                   <  1:161040/149‑3 (MQ=255)
              cagcagCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcat                                                                                                                                   <  1:211374/149‑3 (MQ=255)
              cagcagCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAGGcat                                                                                                                                   <  1:223488/149‑3 (MQ=38)
                 cagcCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAGGCCTGATAAGACGCGACCGCGTCGCATCAGGcatcgg                                                                                                                                <  2:255755/149‑6 (MQ=37)
                    ccACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                                                                                             <  2:286757/149‑1 (MQ=255)
                    ccACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                                                                                             <  2:318484/149‑1 (MQ=255)
                     cACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc                                                                                                                            >  1:301219/1‑149 (MQ=255)
                          tAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGCCGCGTCTTATCAGGCCTACAGGTCGATGc                                                                                                                       <  1:476420/149‑1 (MQ=255)
                            gCTCGACCAATGGTCGGATTAACCGCGATAAAGCGTGGCGCGGTCCGGGCGCTTGGACGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCg                                                                                                                     <  1:343358/149‑1 (MQ=21)
                                gACCAATTGTCTGATTAACCGCTATAACGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGatat                                                                                                                 <  1:211286/149‑1 (MQ=255)
                                gACCAATGGTCTGATTAACCGCTATAAGGCGTGGCGTCGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGTTCGATGCCGattt                                                                                                                 <  2:748156/149‑3 (MQ=255)
                                 aCCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGCCGATGCCGatagg                                                                                                                >  1:244010/1‑147 (MQ=255)
                                       ggTCGGATCAACCGTGAGAAGGCGTGGCGAGGTTAATCAAAGAGGATATATAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAGGCGTGAGGCGACGCGGTCGCGTCTTATCAGGCTTACAGGTCGATGCCGATATGTACATc                                                                                                          <  2:512064/149‑1 (MQ=21)
                                         tCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGt                                                                                                        >  2:550557/1‑149 (MQ=255)
                                         tCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGt                                                                                                        >  2:543359/1‑149 (MQ=255)
                                            gATTAACCGCTATAAAGCCTGGCGCGGTTAACCATCGTCGACACGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt                                                                                                     <  1:320646/149‑1 (MQ=37)
                                             aTTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc                                                                                                    >  2:322389/1‑149 (MQ=255)
                                                aaCCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                 <  2:261615/149‑1 (MQ=255)
                                                 aCCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCa                                                                                                <  2:592423/149‑1 (MQ=255)
                                                 aCCGCGATAAAGCGGGGCGCGGTTAAGCATCGTCGATATGTGGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCa                                                                                                <  2:72956/149‑1 (MQ=255)
                                                  ccGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCaa                                                                                               >  1:49056/1‑149 (MQ=255)
                                                        taAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTCCAGGTCGATGCCGATATGTACATCGTATTCGGCACTTAATa                                                                                         >  1:144994/1‑149 (MQ=255)
                                                        taAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGCTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGGCGGGTCTTATCAGGCATAAAGGTCGATGCCGATAGGTACATGGTAGTCGGCAATTAATa                                                                                         >  2:510964/1‑149 (MQ=37)
                                                         aaaGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc                                                                                        <  1:90303/149‑1 (MQ=255)
                                                         aaaGCGGGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGGGTCTTATCAGGCCTACAGGTCGATGCCGAGATGTACATCGTATTGGGCAATTCAtgc                                                                                        >  2:586433/1‑147 (MQ=37)
                                                            gcggtgcgcGGTTAACCAGCGGCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCGTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATa                                                                                     <  2:127392/144‑1 (MQ=25)
                                                            gCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATa                                                                                     <  2:725513/149‑1 (MQ=255)
                                                            gCGGGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACTTCGTATTCGGCTATTAATACATa                                                                                     <  2:656558/149‑1 (MQ=255)
                                                                   gcgGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgat                                                                              >  2:52251/1‑149 (MQ=255)
                                                                              tcgtcgATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATaa                                                                   >  1:135528/1‑149 (MQ=255)
                                                                              tcgtcgATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAACTACCACGATTGATTAAATaa                                                                   >  2:179329/1‑149 (MQ=255)
                                                                              tcgtcgATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAAATCGTATTCGGCAATTAATACATAGCCGGATTGATTAAATaa                                                                   >  2:9282/1‑149 (MQ=255)
                                                                               ggtcgATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAAc                                                                  <  2:660912/148‑1 (MQ=255)
                                                                                 tcgATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATACCACGATTGATTAAATAACCt                                                                >  1:636743/1‑149 (MQ=255)
                                                                                    atatGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTtaa                                                             <  1:400243/149‑1 (MQ=255)
                                                                                         tAGGCAGGATCAGGCGGTGGCGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACa                                                        >  2:289377/1‑149 (MQ=38)
                                                                                             ccGGATAAGGCGTTCACGCGGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGc                                                    <  1:195169/149‑1 (MQ=255)
                                                                                                      gCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTAt                                           >  2:658057/1‑149 (MQ=255)
                                                                                                      gCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAAAGTTAATAACACTGCGGAGGTTAt                                           >  2:214723/1‑149 (MQ=38)
                                                                                                       cGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAATCTTAATAACAATGCCGACGTTATg                                          <  1:583598/149‑1 (MQ=255)
                                                                                                       cGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATg                                          <  1:582756/149‑1 (MQ=255)
                                                                                                       cGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATg                                          <  1:231556/149‑1 (MQ=255)
                                                                                                        gTTGACGCCGCAGCCGGCAACCCATGCCTGACGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACAGCTGATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGt                                         <  2:497382/149‑1 (MQ=38)
                                                                                                        gTTCACGCCGCAGCCGGCAACCGATGCCTGACGCGACGCGGTCGCGTTTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGt                                         <  2:236878/149‑1 (MQ=255)
                                                                                                         ttCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATAAGGCATACAAGGGAAGGCCGAAATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGGCGTTCTGTc                                        >  2:285070/1‑149 (MQ=255)
                                                                                                           cACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCgg                                      >  1:550589/1‑149 (MQ=255)
                                                                                                              gccgcATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATCACAATGCCGACGTTATGTCGGcct                                   >  1:279124/1‑147 (MQ=255)
                                                                                                                   aTCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCAtttttt                              >  2:734540/1‑149 (MQ=255)
                                                                                                                   aTCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATCCCGACGTTATATCGGCAtttttt                              >  2:486829/1‑149 (MQ=255)
                                                                                                                   aTCCAGCAACCGATGCCTGATGCGACGCAGTCGCGGCTTATCAGGCCTACAGGTCGATGCCGGTATATACATCGTATTCGGCAATTAATACATAGCACGATTGTTTAAACAACCTTAATAACCATGCCGCCGTTATGTCGGCAtgtttt                              >  2:604740/1‑149 (MQ=255)
                                                                                                                     ccGGCAAACGATGCCTGATGCGACGCGGTCGCCTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTa                            <  1:44767/149‑1 (MQ=255)
                                                                                                                          gaCCGATGCCTGATGCGACGCGGGCGCGGCGTATCAGGCCTACAGGTCGATGGCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAAGGCCGACGTTATGTCGGCATTTTTTTATCAGa                       <  2:99060/148‑1 (MQ=255)
                                                                                                                             cGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGAGATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATACCGACATTATGTCGGCATTTTTTTATCAGATaa                    >  1:445526/1‑149 (MQ=255)
                                                                                                                              taggCCTGATAAGACGCGACCGCGTCGCATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATaaa                   >  1:564412/4‑149 (MQ=38)
                                                                                                                              taggCCTGATAAGACGCGACCGCGTCGCATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATaaa                   >  1:605918/4‑149 (MQ=38)
                                                                                                                                   cTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATcccc              <  1:543359/149‑1 (MQ=255)
                                                                                                                                   cTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTACGTCGGCATTTTTTTATCAGATAAATcccc              <  1:550557/149‑1 (MQ=255)
                                                                                                                                   cTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGCACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATcccc              <  1:227361/149‑1 (MQ=255)
                                                                                                                                             gcgGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACCTCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACCATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTaa    <  2:50713/149‑1 (MQ=255)
                                                                                                                                              cgGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACCTCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGAAGATATGGCGGCATTTTTTTATCAGATAAATCTCCTTGTTTGTAAt   >  2:377899/1‑149 (MQ=255)
                                                                                                                                               ggTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAAtt  <  2:660009/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATT  >  NC_000913/4235332‑4235623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑