Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,618,566 C→T 6.0% T264T (ACC→ACT nikE → nickel ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,618,5660CT 6.0% 187.2 / 2.9 69T264T (ACC→ACTnikEnickel ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (37/27);  new base T (2/2);  total (40/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01

AGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAG  >  NC_000913/3618419‑3618713
                                                                                                                                                   |                                                                                                                                                   
aGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGcacca                                                                                                                                                    <  1:180380/149‑1 (MQ=255)
      cTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTCCCCGCCTTCCCCGTGCGCCGTCGCCCCTCAGaaa                                                                                                                                              >  2:30843/1‑149 (MQ=255)
       ttttGCCAGCGGGTAATGGTTATGGACAACGGCGATCTCGGCCGAAGCCAGGTGGTGGGAGAGAAATTAACCTTTCCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGaaaa                                                                                                                                             <  1:532216/149‑1 (MQ=255)
       ttttGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAGTTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGAATTACCCGAATTCCGCGTGAGCGGGCGAGCCACAGaaaa                                                                                                                                             >  1:197665/1‑149 (MQ=255)
       ttttGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGaaaa                                                                                                                                             <  1:538075/149‑1 (MQ=255)
           gCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGtt                                                                                                                                         <  1:697590/149‑1 (MQ=255)
             cAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTa                                                                                                                                       >  1:6758/1‑149 (MQ=255)
              aGCGGGTAATGGTGATGGACAACGGACCAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTaa                                                                                                                                      <  1:291311/149‑1 (MQ=255)
               gCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACGCCAGAAATGGTTTAAc                                                                                                                                     >  1:206251/1‑149 (MQ=255)
               gCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAAc                                                                                                                                     >  1:613289/1‑149 (MQ=255)
               gCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAAc                                                                                                                                     >  1:557711/1‑149 (MQ=255)
               gCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAAc                                                                                                                                     >  1:81724/1‑149 (MQ=255)
                 gggTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGc                                                                                                                                   >  2:486082/1‑149 (MQ=255)
                    tAATGGTTATGGACAACGGACAAATCGTCGAATCCCAGGGGGTGGGAGAGAACTCAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGTTATTACCCGCATTCCCCGTGCGCTGTCGCACCACAGAAAAGGTTTAACGCaaa                                                                                                                                <  2:431704/149‑1 (MQ=255)
                      aTGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAACACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATg                                                                                                                              >  1:374656/1‑149 (MQ=255)
                         gTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCaa                                                                                                                           <  1:124162/149‑1 (MQ=255)
                           tATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACg                                                                                                                         >  2:243039/1‑149 (MQ=255)
                             tGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAg                                                                                                                       >  2:414902/1‑149 (MQ=255)
                               gACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTc                                                                                                                     <  2:463655/149‑1 (MQ=255)
                                aCAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACTCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCa                                                                                                                    <  2:438364/149‑1 (MQ=255)
                                    ctgACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCAt                                                                                                                >  2:331955/3‑149 (MQ=255)
                                     ggACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTAACCATc                                                                                                               >  1:79826/1‑149 (MQ=255)
                                     ggACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGACAcccac                                                                                                               >  2:424145/1‑146 (MQ=255)
                                     ggACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGGTTAACGCAAATGACAAGAGTCACCATc                                                                                                               >  2:36102/1‑149 (MQ=255)
                                         aaaTCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGc                                                                                                           >  1:332240/1‑149 (MQ=255)
                                         aaaTCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGCCACCATCACGc                                                                                                           >  1:724825/1‑149 (MQ=255)
                                           aTCGTCGAAACCCAGGTGGTGGGAGAGAAATTAGCCTGTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGTTATTACCCGCATTCCCCGTTCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCtt                                                                                                         <  2:738474/149‑1 (MQ=255)
                                            tcgtcgAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCCCGCTtg                                                                                                        >  2:89405/1‑149 (MQ=255)
                                                  aaaCCCAGGTGGTGGGAGAGAAAATAACCTATTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTAACCGCATTCCCCGTGAGCCGTCGCACCAGAGAAAAGGTTTAACGCAACTGCAACGAGGCCCCAACACCCTTGAAGAca                                                                                                  >  2:536495/1‑148 (MQ=255)
                                                  aaaCCCAGGTGGGGGGAGAGAAATTAACCTTGTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACg                                                                                                  <  2:464518/149‑1 (MQ=255)
                                                  aaaCCCAGGGGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACg                                                                                                  <  1:327580/149‑1 (MQ=255)
                                                   aaCCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGa                                                                                                 >  1:778479/1‑149 (MQ=255)
                                                     cccAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGAtt                                                                                               <  2:502594/149‑1 (MQ=255)
                                                        aGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTAc                                                                                            >  1:320583/1‑149 (MQ=255)
                                                          gtggtgGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGGGTCACCATCACGCTTGATGACGATTTACTg                                                                                          >  1:6817/1‑149 (MQ=255)
                                                          gtggtgGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTg                                                                                          >  1:480016/1‑149 (MQ=255)
                                                                  agagaAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAACACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCt                                                                                  <  2:750301/149‑1 (MQ=255)
                                                                   gggaAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTg                                                                                 <  1:599813/147‑1 (MQ=255)
                                                                   gataacttAACCTTTCCCTCTGACGCCGGACGTGTGCTACAAAACGCGCCAGTTCCCGCGTGCCCCGTGCGCCGTCGCACCACAGAAAAGGCTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTg                                                                                 <  1:181521/143‑1 (MQ=255)
                                                                      aaaTTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACTACAGAAAAGGTTTAACGCAAATGCAACGAGTGACCATCACGCTTGATGACGATTTACTGGAGACGCTGGAc                                                                              >  1:555440/1‑149 (MQ=255)
                                                                      aaaTTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACTACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGAc                                                                              >  1:678153/1‑149 (MQ=255)
                                                                          tAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGcc                                                                          <  1:331955/149‑1 (MQ=255)
                                                                                 ttCCTCGGACGCCGGACGTGTGCCACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGCGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCa                                                                   <  2:131039/149‑1 (MQ=255)
                                                                                     tctGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGcgt                                                               >  1:556954/1‑149 (MQ=255)
                                                                                     tctGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGcgg                                                               >  1:738314/1‑148 (MQ=255)
                                                                                      ctGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGcgtc                                                              >  2:702040/1‑149 (MQ=255)
                                                                                       tGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAACGGTTTAACGCAACTGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGCCTCTGGACAGCCTGAGCCAGcgtcg                                                             <  2:7381/149‑1 (MQ=255)
                                                                                       tGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGCCACCATCACGCTTGATGACGCTTTACTGGAGACGCTGGACAGCCTGAGCCCGcgtcg                                                             >  1:691068/1‑149 (MQ=255)
                                                                                             cGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTta                                                       >  1:397639/1‑149 (MQ=255)
                                                                                                       cTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGt                                             >  1:658255/1‑149 (MQ=255)
                                                                                                       cTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAAGTTTAACGCAAATGCAACGCGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCACCGTCGTGGTTATAACAACCGt                                             >  1:331246/1‑149 (MQ=255)
                                                                                                         agaaaaCGCCGCATTACCCGCATTCCCCGTCCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGAGGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTc                                           <  2:59803/147‑1 (MQ=255)
                                                                                                         aCAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCAACACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTc                                           >  1:561822/1‑149 (MQ=255)
                                                                                                             accgccGTATTCCCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATTACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGaa                                       <  2:478691/147‑1 (MQ=255)
                                                                                                             aaCGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGaa                                       >  1:702221/1‑149 (MQ=255)
                                                                                                              aCGCGTTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAg                                      <  1:249934/149‑1 (MQ=255)
                                                                                                                  ggTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGAGGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACACCAGTTCCGAGACTAt                                  >  2:222775/1‑149 (MQ=255)
                                                                                                                       tACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCcgcg                             <  1:688001/149‑1 (MQ=255)
                                                                                                                          ccGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAGGCTATCCGCGACa                          >  1:354521/1‑149 (MQ=255)
                                                                                                                              aTTCCCCGTGCGCCGTCGCACTACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGATATCCGGGACATTCt                      <  2:678153/149‑1 (MQ=255)
                                                                                                                              aTTCCCCGTGCGCCGTCGCACTACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCAGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCt                      <  2:555440/149‑1 (MQ=255)
                                                                                                                              aTTCCCCGGCCGCCGCCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGACGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCTTCGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCt                      <  2:6758/149‑1 (MQ=255)
                                                                                                                               ttCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGTATAGCTTGCGTCCACGTTGGGGTTATAACAACCGTTCCGAATCTATCCGCGACATTCTg                     <  1:379508/149‑1 (MQ=255)
                                                                                                                               ttCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAAGAACCGTTGCGAGGCTTACCGGCGCATTCTg                     >  1:731266/1‑149 (MQ=255)
                                                                                                                               ttCCCCGTGCGCCGTCACACCCCAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTg                     <  2:663827/149‑1 (MQ=255)
                                                                                                                                cccccGTGCGCCGTCGCACCACAGAAAAGGTTTAACCCAAACGCATCCATTCACCATCACGCTTGCTGACAATTAACCGCAGAACCTGTACAGCCTGATCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGc                    <  2:197665/148‑1 (MQ=255)
                                                                                                                                       gcgcCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAgcgc             <  1:414902/149‑1 (MQ=255)
                                                                                                                                        cgcCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCACCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGcc            >  1:745519/1‑149 (MQ=255)
                                                                                                                                           cGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTg         >  1:235721/1‑149 (MQ=255)
                                                                                                                                           cGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTAACCGTCACGCTTACTGACGCTTTACTGGAGACGCTGGACAGCATGAGCACGCGCCGTGGCAATAAAAAACGTTCCGAAGCGCTCCGCGAGCTCCTGACTAGCGCCCTg         >  2:391969/1‑149 (MQ=255)
                                                                                                                                                caccacAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGgcgcc    >  2:630331/1‑148 (MQ=255)
                                                                                                                                                  ccacAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAag  <  2:613289/149‑1 (MQ=255)
                                                                                                                                                  ccacAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAag  <  2:206251/149‑1 (MQ=255)
                                                                                                                                                  ccacAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAag  <  2:81724/149‑1 (MQ=255)
                                                                                                                                                  ccacAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGATCTGTGCTACATTCTGCGTAGCGCCCTGGCGCAag  <  2:557711/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAG  >  NC_000913/3618419‑3618713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: