| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,598,439 | T→C | 12.2% | intergenic (+73/+115) | panM → / ← livJ | PanD autocleavage accelerator, panothenate synthesis/branched‑chain amino acid ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,598,439 | 0 | T | C | 12.2% | 105.1 / 7.1 | 49 | intergenic (+73/+115) | panM/livJ | PanD autocleavage accelerator, panothenate synthesis/branched‑chain amino acid ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base T (26/17); new base C (3/3); total (29/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01 | |||||||||||
TCGCGGTGTGATGACGGCGTTTATGCAGGCGCTGGGGTTTACGGCACAACAGGGCGGCTGGGAGAAGTGTTAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGC > NC_000913/3598294‑3598587 | tCGCGGTGTGATGACGGCGTTTATGCAGGCGCTGGGGTTTACGGCACAACAGGGCGGCTGGGAGAAGTGTTAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAATTTa > 2:486288/1‑149 (MQ=255)tCGCGGTGTGATGACGGCGTTTATGCAGGCGCTGGGGTTTACGGCACAACAGGGCGGCTGGGAGAAGTGTTAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGATTATCAGGCCTACAATGTGTGTTGCGATTTa > 2:219027/1‑149 (MQ=255)tCGCGGTGTGATGACGGCGGTTATGCAGGCGCTGGGGTTTACGGCACAACAGGGCGGCTGGGAGAAGTGTTAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAATTTa < 2:602945/149‑1 (MQ=255) atgaCGGCGTTTATGCAGGCGCTGGGGTTTACGGCACAACAGGGCGGCTGGGAGAAGTGTTAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCtt < 1:354431/149‑1 (MQ=255) tATGCAGGCGCTGGGGTTTACGGCACAACAGGGCGGCTGGGAGAAGTGTTAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTa < 2:578619/149‑1 (MQ=255) cAGGGGCTGGGGTTTACGGCACAACAGGGCGGCTGGGAGAAGTGTTAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGcc < 1:207007/149‑1 (MQ=255) aGGCGCTGGGGTTTACGGCACAACAGGGCGGCTGGGAGAAGTGTTAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGTTCTTGTAGGCCg > 2:40459/1‑149 (MQ=255) cgcTGGGGTTTACGGCACAACAGGGCGGCTGGGAGAAGTGTTAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGAt > 2:705634/1‑149 (MQ=255) tGGGGTTTACGGCACAACAGGGCGGCTGGGAGAAGTGTTAATGGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAg > 2:472546/1‑149 (MQ=255) tGGGGTTTACGGCACAACAGGGCGGCTGGGAGAAGTGTTAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACGATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGCTAAg > 1:88406/1‑149 (MQ=255) tGGGGTTTACGGCACAACAGGGCGGCTGGGAGAAGTGTTAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACGATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAg > 1:99795/1‑149 (MQ=255) gggTTTGCGGCACGACGGGGCGGCTGGGAGAAGTGTTAATCGTCAAGTTTGATTTCAAAAGTGATATGGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGc < 2:500058/149‑1 (MQ=255) gTTTACGGCACAACAGGGCGGCTGGGAAAAGTGTTAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTAAGATTATCAGGCCTGCAATGTGTGTTGCAATGTACTGGTTTAGATCGATCTTGTAAGCTGGATAAGGCgg > 2:5623/1‑148 (MQ=255) aCGGCACAACAGGGCGGGTGGGAGAAGTGTTAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTAc < 1:540188/149‑1 (MQ=255) ggCACAACAGGGGGGCTGGGAGAAGTGTGAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTAcgc < 1:548828/149‑1 (MQ=255) gCACAACAGGGCGGCTGGGAGAAGGTGTAGTCTTCAAGTGTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGTCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTAcgcc < 1:390446/149‑1 (MQ=38) tgtTAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAGTTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCCTGAAGACACGTACt > 2:454731/1‑149 (MQ=255) aTCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAACTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGata > 2:584380/1‑149 (MQ=255) aTCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAACTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGata > 2:701151/1‑149 (MQ=255) aTCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAACTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGata > 2:124822/1‑149 (MQ=255) tttGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAAtt > 2:221757/1‑149 (MQ=255) tttGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCGACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGACGCATCCGGCATGAAGCCACGTAAGCGATATTAGCAAtt > 2:45308/1‑149 (MQ=38) tttCAAAAGTGATATTGCCTGATGCGCTACGCTGATCAGGCCTACAATGTGTTTTGCAATTTATTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCTGCGTGAAGCAACGTACTCGATATTAGCAATTTggcg < 2:608188/149‑1 (MQ=37) tCAAAAGTGATATTGCCTTGTGCGCTACGCTTATCAGCCCTACCGCGTTGGGTGCCCTTTAATGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACCCGAGATTAGCAATTTggcggc > 1:574239/1‑149 (MQ=25) tGCCTGATGCGCTCCGCTTATCAGGCCTACACTGTGTGTTGCAATTTACTGACCTCCTCGGACCTCCTACGCCGGCTAAGGCGTCTCCGCCGCCCCCGCCATGAAGCAACGTACCCGCCACTAGCAATCTGCCCGCCACCCCAAGtccc > 2:509839/1‑146 (MQ=255) cTGATGGGCCACGCTTTTCAGGCCTACAATGTGTGTTGCAATCTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTACCAATTTGGCGGCAACCCAAAGTTGCCGa < 1:419395/149‑1 (MQ=38) gcTACGCTTATCTGGCCTACAATGTGGGTTGGAATTTACTGATTTCTTTGGAACATGTAAACCGTATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGa < 1:182486/149‑1 (MQ=255) gcTACGCTTATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCACCCCAAAGTTGCCGATCAATgc > 1:447079/1‑148 (MQ=255) tACGCTTATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGCTAAGGCGTTTACGCCGCATCCGGCCTGGCGCAACGTACTCGATAATAGAAATCTGGCGGCAACCCCACGCTGCCTATTAATGCtt > 2:154436/1‑149 (MQ=37) aCGCTTATCAGGCCTACAAGGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTa < 1:275557/149‑1 (MQ=255) gCTTATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACt > 2:21917/1‑149 (MQ=255) tATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCg < 1:705634/149‑1 (MQ=255) aTCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATCAATGATATCTTCGc > 2:423515/1‑149 (MQ=255) tCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCa < 1:194232/149‑1 (MQ=255) ggCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCg > 1:395584/1‑149 (MQ=255) gCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCgg < 1:301228/149‑1 (MQ=255) tACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGGTTAATGATATCTTCGCATCGGTCg > 1:596748/1‑149 (MQ=255) tACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCg > 2:597912/1‑149 (MQ=255) tACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAATTGCCGATTAATAATTACTTCCAATCGGTcc > 1:668082/1‑148 (MQ=255) aaTGTGTGTTGCATTTTCCTGCTTTCTTTGGACCTTGTAGGCCGGAAAAAGGGTTTGCGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCgccg < 1:219027/149‑1 (MQ=255) aaTGTGTGTTGCAATTTGCTGATTTCTTTGGATCGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCgccg < 1:486288/149‑1 (MQ=255) tgtTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGTTCgccgtgccgt > 2:316374/1‑149 (MQ=255) tgtTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCgccgtgccgt > 2:780192/1‑149 (MQ=255) gtTGCAACTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGtt < 1:584380/149‑1 (MQ=255) gtTGCAACTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGtt < 1:124822/149‑1 (MQ=255) gtTGCAACTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGtt < 1:701151/149‑1 (MQ=255) gCAATTTACTGATTTCTTTGGGTCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGc > 2:255984/1‑149 (MQ=255) cAATTTACTGATTTCTTTGGCTCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCCCCGTGCCGTTGGCg > 1:384373/1‑149 (MQ=255) cAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAACGTTGCCGCTTAATGGTTGCTTCGCATGGGTGGCCGTGCCGTTGGCg > 1:719140/1‑149 (MQ=38) tttACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGc < 1:634888/149‑1 (MQ=255) | TCGCGGTGTGATGACGGCGTTTATGCAGGCGCTGGGGTTTACGGCACAACAGGGCGGCTGGGAGAAGTGTTAATCGTCAAGTTTGATTTCAAAAGTGATATTGCCTGATGCGCTACGCTTATCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGC > NC_000913/3598294‑3598587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |