Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,867,661 T→C 8.5% C252R (TGC→CGC)  yeaG → protein kinase, endogenous substrate unidentified; autokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,867,6610TC 8.5% 103.7 / 3.6 48C252R (TGC→CGC) yeaGprotein kinase, endogenous substrate unidentified; autokinase
Reads supporting (aligned to +/- strand):  ref base T (18/25);  new base C (2/2);  total (20/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.23e-02

TCTGCAACAAATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCG  >  NC_000913/1867516‑1867808
                                                                                                                                                 |                                                                                                                                                   
tCTGCAACAAATTGCTACCCCCAAAACGGACACCGGCGCTGAGAACAACCAGGCCACCTCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGcc                                                                                                                                                  <  2:738823/149‑1 (MQ=255)
   tgaacaaAGTGCCGCCGCCAAAACGGACCCCGGCGATGCGACCAACCAGGGCGCCTCCGCGCGGGGTGGGAAAGTCGGTATGCGGTAACTCGAAGACTACGCGCAGGAGGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCcgcg                                                                                                                                               <  2:457193/147‑1 (MQ=255)
     aacaaATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCGGGTGCGCTGTGCCGCGcc                                                                                                                                             >  1:596399/1‑149 (MQ=255)
     aacaaATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGcc                                                                                                                                             >  1:682088/1‑149 (MQ=255)
             gCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGATGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAAACAggg                                                                                                                                     >  2:213335/1‑149 (MQ=255)
                 tCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATc                                                                                                                                 >  1:483042/1‑149 (MQ=255)
                    ccAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATg                                                                                                                              <  1:691256/149‑1 (MQ=255)
                       agagggAACCCGGGGATGAGAACAACCAGGACATCCCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACGCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGaa                                                                                                                           <  2:463722/145‑1 (MQ=255)
                       aaaCGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGaa                                                                                                                           >  2:703428/1‑149 (MQ=255)
                         aCGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAAtt                                                                                                                         <  2:479771/149‑1 (MQ=255)
                             aaCCCGGCGATGAGAACATCCAGGCCCTCTCCGCGCTGGCTGGGACAGTCGATAGTCGTAAACTCGGGCGCTACGCGCAGAATGACCCGGTCTCGTACGGCTATTGCGGTTCGGTGGGCCGCGCCAATCAGGGGATCATGGAATTCGtt                                                                                                                     <  2:265037/149‑1 (MQ=255)
                             aaCCCGGCGATGAGAACAACCAGGGCATCTCCTCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGGAGAATGACGCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGTGCCAATCAGGGGATCATGGAATTCGtt                                                                                                                     <  2:400301/149‑1 (MQ=255)
                              aCCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTg                                                                                                                    <  1:337258/149‑1 (MQ=255)
                              aCCCGGCGATGAGAACAACAAGGACCTCCCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTg                                                                                                                    <  2:629030/149‑1 (MQ=255)
                                   gCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATg                                                                                                               >  1:58265/1‑149 (MQ=255)
                                   gCGATGAGAACAACCAGGACATCTCCGCGCTGATTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATg                                                                                                               >  1:736411/1‑149 (MQ=255)
                                     gactagaACAACCAGGACATTTCCGCGCTGGTTGGGAAAGTCGATATGCGTAAACTCGAACACTACGCGCAGAATGACCCGTACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCTAATCAGGTGATCATGGAATTCGTTGAGATGtt                                                                                                             <  2:27765/145‑1 (MQ=255)
                                     gATTAGATCAACCAGGACATCTCCGCGCCGGTTGGGAAACTTGATATTCGGAAACTCGAACACGACGCTCAGAATGACCCGTACCCCTACGGCTATTCCGGTGCGCTGTGCCGCTCCAATCAGGGGATCATGGAATTCGTTGAGCTGtt                                                                                                             <  2:441680/149‑1 (MQ=255)
                                      taccaaacaacCAGGACATCTCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGCGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGttt                                                                                                            >  2:292521/6‑149 (MQ=255)
                                      aTGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGCGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGttt                                                                                                            >  2:478929/1‑149 (MQ=255)
                                          gaACAACCAGGACATCTCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAg                                                                                                        <  1:725138/149‑1 (MQ=255)
                                          gaACAACCAGGACATCTCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAg                                                                                                        <  2:571265/149‑1 (MQ=255)
                                                     aCATCTCCGCGCTGGGTGGGAAAGTCGGTGTTCGTAAACTCGCACCCTACGCGCAGAATGACCCGGTCGCCTACGGTTATTCCGGGGGGCTGTGCCGCGTCAGTCAGGGGATCGTGGTATTCGTTGAGATGTTTAAAGCACCGATTaaa                                                                                             <  2:514218/149‑1 (MQ=255)
                                                          tcCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGCTCATGGAATTCGTTGAGATGTTTAACGCACCGATTAAAGagct                                                                                        >  2:149322/1‑149 (MQ=255)
                                                          tcCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGAACAAGGAAACCGATGAGATGTTAAAAGCACCCAATAAAGtact                                                                                        >  1:58161/1‑149 (MQ=255)
                                                            cgcgCTGGGTGGGAAAGTCGATATTCGTAAACTCGAACCCTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGtgctgc                                                                                      >  1:352284/1‑149 (MQ=255)
                                                                        gAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCGCCGACTAAAGTGCTGCATCCCTTGTTaa                                                                          <  1:430355/149‑1 (MQ=255)
                                                                              cGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCa                                                                    <  2:116176/149‑1 (MQ=255)
                                                                                      gTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGaa                                                            <  1:449306/149‑1 (MQ=255)
                                                                                      gTAAACCAGTACACTACGCGCAGAATGACCCGGACGCCTACGGCTATCCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGaa                                                            <  1:449235/149‑1 (MQ=255)
                                                                                              gAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACtg                                                    >  2:764788/1‑148 (MQ=255)
                                                                                                     aCGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCCGGAAGGTAGCTACAACggg                                             >  2:295240/1‑149 (MQ=255)
                                                                                                     aCGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGCAGGTAACTACCCCggg                                             >  2:640381/1‑149 (MQ=255)
                                                                                                     aCGCGCAGAATGACCCGGAAGCCTACGGCTATTCCGATGCGCTGTGCCGCGCCAATCAGGGGAGCATGGAATTCGTTGAGATGTGTCAAGCGACGATTAAAGTGCTGCCTCCCTTTTTAATCGCCACTCAGGAAGGTAACTACAACggg                                             >  2:133431/1‑149 (MQ=255)
                                                                                                      cgcgCAGAATGCCCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGa                                            <  2:155493/149‑1 (MQ=255)
                                                                                                      cgcgCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGa                                            <  1:593958/149‑1 (MQ=255)
                                                                                                      cgcgCAGAACGAGCCGGCCGCCTACGGCTATGTCGGTTCCGTGTGCCGCGCCAATAATTGGATCATGGAACTCCTTGAAATGTTTAACGCACCGATTAAAGTGCTGCATCCCTGGTTAACCGCCACTCAGGAATGTAACTACAACGGGa                                            <  2:91531/149‑1 (MQ=255)
                                                                                                                  cccGGACGCCTACGGCTATTCCGGTGCGCTGCGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGCAGGTAACTACAACGGGACGGAAGGTAtct                                <  1:478929/149‑1 (MQ=255)
                                                                                                                  cccGGACGCCTACGGCTATTCCGGTGCGCTGCGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTAtct                                <  1:292521/149‑1 (MQ=255)
                                                                                                                           cTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGCTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGGAGGTATCTCCGCCCTGc                       >  1:311097/1‑149 (MQ=255)
                                                                                                                               ggCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGAATCCCCGCCCTGCCGtt                   >  2:650959/1‑149 (MQ=255)
                                                                                                                                   aTTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAAc               <  1:747318/149‑1 (MQ=255)
                                                                                                                                    ttCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGGGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACg              <  1:226135/149‑1 (MQ=255)
                                                                                                                                      ccGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACggg            >  1:742498/1‑149 (MQ=255)
                                                                                                                                       cGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGa           <  2:58265/149‑1 (MQ=255)
                                                                                                                                       cGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGa           <  1:704331/149‑1 (MQ=255)
                                                                                                                                       cGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGa           <  2:736411/149‑1 (MQ=255)
                                                                                                                                        ggTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGat          >  2:580192/1‑149 (MQ=255)
                                                                                                                                               tgtgCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAACGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATtctc   >  2:49981/1‑149 (MQ=255)
                                                                                                                                                gtgCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCg  <  1:93930/149‑1 (MQ=255)
                                                                                                                                                gtgCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCg  <  1:434156/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TCTGCAACAAATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAAGTCGATATTCGTAAACTCGAACACTACGCGCAGAATGACCCGGACGCCTACGGCTATTCCGGTGCGCTGTGCCGCGCCAATCAGGGGATCATGGAATTCGTTGAGATGTTTAAAGCACCGATTAAAGTGCTGCATCCCTTGTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCG  >  NC_000913/1867516‑1867808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑