Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908 258683 776 47 [0] [0] 47 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)41 (0.990) 31/260 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

GCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913/257761‑258055
                                                                                                                                                  |                                                                                                                                                    
gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAag                                                                                                                                                    >  1:517084‑M1/1‑147 (MQ=255)
 cTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagt                                                                                                                                                   <  1:457680‑M1/149‑4 (MQ=255)
 cTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGGATAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagt                                                                                                                                                   >  2:169463‑M1/1‑146 (MQ=255)
         aaaaaTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagat                                                                                                                                           >  2:25000‑M1/1‑138 (MQ=255)
           aaaTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataa                                                                                                                                         >  2:45740‑M1/1‑136 (MQ=255)
                    aaaTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctt                                                                                                                                <  1:365682‑M1/149‑23 (MQ=255)
                     aaTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCCCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttt                                                                                                                               >  1:532817‑M1/1‑126 (MQ=255)
                          tGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcga                                                                                                                          >  1:297360‑M1/1‑121 (MQ=255)
                            ccAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCTCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgatt                                                                                                                        <  1:43698‑M1/149‑31 (MQ=255)
                             cAATTTGGTAAAACAGTTGCATCAGAACAGGAGATAGCAATGACGTTCCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTGGGCCCGGATATTCGTGAAGGTAagtgcaaagcaaatcgatccttattcgattg                                                                                                                       >  2:705036‑M1/1‑118 (MQ=255)
                                  tGGTAAAACAGTTGCAACACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg                                                                                                                  <  2:331744‑M1/149‑37 (MQ=255)
                                           agctgcaACGCAACAGGAGATAGCAATGACGTTACCGAGTGGACCCCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGGTAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcg                                                                                                         <  2:417285‑M1/146‑46 (MQ=255)
                                               gCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACACGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGACCGTAGATGCGTGAGGGTAagtgcaaagctaatagattctttttgggttgtctggctttttgcgtcag                                                                                                     >  1:91953‑M1/1‑100 (MQ=255)
                                               gCATCACAAAAGGAGATAGCAATGACGTTAACGCGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataataggttctttttggattgtctggctgtatgcgtcaa                                                                                                     >  2:549945‑M1/1‑100 (MQ=255)
                                                   cacaACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGGAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtg                                                                                                 >  2:459643‑M1/1‑96 (MQ=255)
                                                         aggcgttagcaagGAAGTTACCGGGTGGACACCCGAAGAGCAGATTGATAAAAAAATTTTCCGCACTTGGCCCGTATATTCGTGAAGGTAagggcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccg                                                                                           <  2:124952‑M1/143‑60 (MQ=255)
                                                                     tGACGTTACCGAGTGGCCATCCTAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtaaacgtgaaaccggcaccggaagtg                                                                               <  1:580129‑M1/149‑72 (MQ=255)
                                                                        cGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggtaccggaagtgcgt                                                                            <  1:536567‑M1/149‑75 (MQ=255)
                                                                            aCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaat                                                                        >  2:633821‑M1/1‑71 (MQ=255)
                                                                            aCCGAGGGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatagattctatttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaag                                                                        >  2:230096‑M1/1‑71 (MQ=255)
                                                                               gAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagctaatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccgaaagcggaagtgcgtgaattct                                                                     >  1:59849‑M1/1‑68 (MQ=255)
                                                                                    gACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttctattgtttggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggc                                                                >  2:524253‑M1/1‑63 (MQ=255)
                                                                                       acCCGAAGAGCAGATTGAACAAAAAATCTACCGCACTGTGTCCGTACAATTGTGAATGGAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctgg                                                             <  1:492591‑M1/149‑90 (MQ=255)
                                                                                         ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgggtgaattctggggcgggtg                                                           >  1:19904‑M1/1‑58 (MQ=255)
                                                                                          cGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgacaccggcaccggaagtgcgtgacttctggggctggtgg                                                          >  2:84508‑M1/1‑57 (MQ=255)
                                                                                          cGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATAACAGTGAAGGGAagtgcgaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgggtgaattcgggggctggtgg                                                          >  2:515740‑M1/1‑57 (MQ=255)
                                                                                           gAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgga                                                         <  1:169463‑M1/149‑94 (MQ=255)
                                                                                           gAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgga                                                         <  2:526203‑M1/149‑94 (MQ=255)
                                                                                           gAAGAGCAGATGGATGAAAAAATTTACCGCACTAGGCCCGTATATTCGGGAAGGTAagtgcaaagataatcgattctttttcgattgtcgggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgga                                                         <  2:600910‑M1/149‑94 (MQ=255)
                                                                                             agagCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccagcaccggaagtgcgtgaattctggggctggtggatg                                                       <  1:676982‑M1/149‑96 (MQ=255)
                                                                                                     ctgATCAAAAAAGTTACCGCAATAGGCCCGTAGATTCGTGAGGGTAagtgcaaagataatcgattcttattcgattgtctggatgtatgcgtcaacgtgaaactggcaccggaagtgcgtgaattctggggctggtggatggagctgga                                               <  1:671633‑M1/148‑104 (MQ=255)
                                                                                                           aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         >  1:621878‑M1/1‑40 (MQ=255)
                                                                                                            aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                        >  2:91972‑M1/1‑39 (MQ=255)
                                                                                                            aaaaaTCTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgatgctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggggctggaagcgcag                                        >  2:681018‑M1/1‑39 (MQ=255)
                                                                                                                  ttACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcc                                  <  1:459643‑M1/149‑117 (MQ=255)
                                                                                                                           tAGGCCCGTATATTCGAGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacc                         >  1:490672‑M1/1‑24 (MQ=255)
                                                                                                                            aGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacct                        >  1:314206‑M1/1‑23 (MQ=255)
                                                                                                                              gCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccagcaccggaagtgcgtgaattctggggctggtggatggagctgcaagcgcaggaatcccgttttacctac                      <  2:397830‑M1/149‑129 (MQ=255)
                                                                                                                               cccGTATTGTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcctcaacgtggaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggatgcgcaggaatcccgttttacctaca                     <  2:7098‑M1/149‑130 (MQ=255)
                                                                                                                               cccGTATATTCCTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctaca                     <  2:91953‑M1/149‑130 (MQ=255)
                                                                                                                               cccGGATATTCGGGAATGTAagtgaaacgttagtggattcggttgcgattgtctggctgcatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctaca                     <  1:549945‑M1/149‑130 (MQ=255)
                                                                                                                                ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgacttctggggctggtggatggagctggaagcgcaggaatcccgttttacctacag                    >  1:574703‑M1/1‑19 (MQ=255)
                                                                                                                                ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacag                    >  2:61776‑M1/1‑19 (MQ=255)
                                                                                                                                ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacag                    >  1:356328‑M1/1‑19 (MQ=255)
                                                                                                                                         cGTTAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcggcaacgtgaaaccggcgccggaagtgcgtgaattctggggctggtggagggcgcgggacgcggcggaatcccgttttacctacagttaccagtt           >  2:689885‑M1/1‑10 (MQ=255)
                                                                                                                                                  aagtgcaacgataaccgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgtt  <  2:450610‑M1/149‑149 (MQ=255)
                                                                                                                                                  aagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgtt  >  1:761036‑M1/1‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913/257761‑258055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.