Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 810,569 G→A 11.5% Q63Q (CAG→CAA)  bioF → 8‑amino‑7‑oxononanoate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913810,5690GA11.5% 170.3 / 9.2 69Q63Q (CAG→CAA) bioF8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand):  ref base G (24/37);  new base A (4/4);  total (28/41)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

GTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTA  >  NC_000913/810424‑810714
                                                                                                                                                 |                                                                                                                                                 
gTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAAAGATTATTTAGGTTTAAGAAATAATCAGAAAATTATCCGTGACTGGcagcag                                                                                                                                                <  2:964742/149‑1 (MQ=255)
 tgctgcCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAgg                                                                                                                                               >  1:346035/1‑149 (MQ=255)
  gctgcCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAAGTTTAAGCCATCATCCGCAAATTATCCGGGCCTGGCAGCAggg                                                                                                                                              >  1:403746/1‑149 (MQ=255)
     gcCGAGTCCCTGGGTAGCCGTTATCCGGTGGCGCAAGGTGCCGGAAGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACGTTTCCAGTAACGATTATGTAGGTTTAAGCCCTCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGc                                                                                                                                           <  1:488955/149‑1 (MQ=255)
      ccGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCg                                                                                                                                          <  1:270751/149‑1 (MQ=255)
       cGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCgg                                                                                                                                         <  2:306600/149‑1 (MQ=255)
        gATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGa                                                                                                                                        <  1:880417/149‑1 (MQ=255)
         aTGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAg                                                                                                                                       >  1:992693/1‑149 (MQ=255)
             ccTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACAATTATTTGGGTTTAAGCCAGCATCCGCAAATTATCCGTGCCTGGCCGACAGGGGCGGAGCaaa                                                                                                                                   >  2:835953/1‑148 (MQ=255)
                    cGCCGTTACCCGGGGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCAt                                                                                                                            <  2:116675/149‑1 (MQ=255)
                       cGTTATCCGGTGGCGCAAGGGGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGTTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCGGGGGGCGGAGCAATTTGGCATCgg                                                                                                                         <  2:430717/149‑1 (MQ=255)
                          gagccGGTGGCGCAAGGGGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAg                                                                                                                      <  2:184084/146‑1 (MQ=255)
                           atacGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTGACGATTATTTAGGTTTAAGCCATCATCTGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAgc                                                                                                                     >  1:852655/4‑149 (MQ=255)
                                gTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGACTGGCAGCAGGGGGCGGGGCAATTTGGCATCGGCAGaggcgg                                                                                                                >  2:463177/1‑149 (MQ=255)
                                     gcAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAACAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCg                                                                                                           >  2:337022/1‑149 (MQ=255)
                                     gcAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAACAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCg                                                                                                           >  2:337021/1‑149 (MQ=255)
                                     gcAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAAAAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCg                                                                                                           >  2:243355/1‑149 (MQ=255)
                                     gcAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATCTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAACAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCg                                                                                                           >  2:337053/1‑149 (MQ=255)
                                         ggAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCa                                                                                                       >  2:680768/1‑149 (MQ=255)
                                          gtgCCGGACGCGGGGTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCAGCATCCGCAAATTATCCATGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCAc                                                                                                      <  2:735389/147‑1 (MQ=255)
                                               ggACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGTCGGAACAATTTAGCATCGGTAGCGGAGGCTCCGGGCACGACAg                                                                                                 >  1:331023/1‑149 (MQ=255)
                                                 aCGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCg                                                                                               <  1:860775/149‑1 (MQ=255)
                                                 aCGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTGAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCg                                                                                               <  2:769946/149‑1 (MQ=255)
                                                    ctggctggTGGGGGGTGATCGCCAGTATCTGAACTTTTCCAGTAACGATTTTTTAGGATTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGtt                                                                                            <  2:222045/149‑1 (MQ=255)
                                                     tggctggTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTta                                                                                           >  2:366787/1‑149 (MQ=255)
                                                      gggtggtggCGTATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCGATTTGGCATCGGTAGCGGTGGCTCCGGTCACGTCAGCGGTtat                                                                                          <  2:419405/146‑1 (MQ=255)
                                                      ggctggTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAAGTTGGCATCGGTAGCGGCGGCGCCGGTCACGTCAGCGGTtaa                                                                                          >  1:331816/1‑148 (MQ=255)
                                                        ctggTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTGAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAg                                                                                        <  1:1094449/149‑1 (MQ=255)
                                                            tggCTGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCgtg                                                                                    <  1:1016572/149‑1 (MQ=255)
                                                            tggCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTGAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCgtg                                                                                    <  2:242622/149‑1 (MQ=255)
                                                              gCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCgtggt                                                                                  >  2:925865/1‑149 (MQ=255)
                                                              gCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCgtggt                                                                                  <  1:261493/149‑1 (MQ=255)
                                                              gCGGATGATCGCCAGTAGCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCgtggt                                                                                  <  1:618591/149‑1 (MQ=255)
                                                               cGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCgtggtg                                                                                 <  2:1113970/149‑1 (MQ=255)
                                                                   tgatCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATc                                                                             >  2:193493/1‑149 (MQ=255)
                                                                   tgatCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATACGAAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGGCGATGCGGGCAGGTCAGGGGGTATAGGggggtgagtt                                                                             >  2:32428/1‑145 (MQ=255)
                                                                      tCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAgg                                                                          >  1:887150/1‑149 (MQ=255)
                                                                         ccAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCAc                                                                       >  1:799057/1‑149 (MQ=255)
                                                                           tggagcTGAACTTTTCCAGTAACGAGTATGTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGTTTTCGGCATTCTGTGTAGCGTGGTGCATCAGGCACTg                                                                     <  2:536096/144‑1 (MQ=255)
                                                                           aGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTTCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTCGCGGCGGCTCCGGTCACGCCAGCGGGTATAGCGTGGTGCATCCGGCCCTg                                                                     >  1:774682/1‑149 (MQ=255)
                                                                                      ttttCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCt                                                          <  2:992693/149‑1 (MQ=255)
                                                                                       tttCCAGTTACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCATTTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCCGCGGTTATCGCGTGGGGCGTCCGGCGCTGGAAGAAGAGCTg                                                         >  2:14942/1‑149 (MQ=255)
                                                                                        ttCCAGTAACGATTATTTAAGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGTATCAGGCACTGGAAGAAGAGCTgg                                                        <  1:853729/149‑1 (MQ=255)
                                                                                           cAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCg                                                     >  2:370753/1‑149 (MQ=255)
                                                                                            aGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGa                                                    >  1:1095590/1‑149 (MQ=255)
                                                                                              tAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGCAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGt                                                  >  1:221140/1‑149 (MQ=255)
                                                                                                  gATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACCGGAAGACGAGCTGGCCGGGtggct                                              >  2:1059246/1‑149 (MQ=255)
                                                                                                   attattTAGGTTTAAGCCTTCTTCCGCTAATTATCCGTGTCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGGAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGtggctt                                             <  1:418819/149‑1 (MQ=255)
                                                                                                   attagtTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGtggctt                                             <  1:1121051/149‑1 (MQ=255)
                                                                                                     tattTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGtggcttgg                                           <  1:111227/149‑1 (MQ=255)
                                                                                                      attTAGGTTTAAGCCATCATCCGCCAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGtggcttggc                                          <  1:229265/149‑1 (MQ=255)
                                                                                                       tttAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCCGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGACGAAGAGCTGGCCGAGtggcttggct                                         >  1:706556/1‑149 (MQ=255)
                                                                                                              ttAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTcgcg                                  <  2:346035/149‑1 (MQ=255)
                                                                                                               tAAGCCATCATCCGCAAATGATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCgg                                 <  1:331794/149‑1 (MQ=255)
                                                                                                                aggCCATCATCCGCAAATTATCCGTGCCGGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCggg                                <  2:162623/147‑1 (MQ=255)
                                                                                                                    catcatCCGCAAATTCTCCGGTCCTTGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCAGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCAct                            <  1:486098/149‑1 (MQ=255)
                                                                                                                         tcCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGt                       <  1:283070/149‑1 (MQ=255)
                                                                                                                               aaTTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGCGGCTTGGCTATTCGCGGGCACTGCTGTTTAtct                 >  2:911704/1‑149 (MQ=255)
                                                                                                                               aaTTATCCGTGCCTGGCAACAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTAtct                 <  1:337021/149‑1 (MQ=255)
                                                                                                                               aaTTATCCGTGCCTGGCAACAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTAtct                 <  1:337022/149‑1 (MQ=255)
                                                                                                                               aaTTATCCGTGCCTGGCAACAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTAtct                 <  1:337053/149‑1 (MQ=255)
                                                                                                                               aaTTATCCGTGCCTGGCAACAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTAGAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTAtct                 <  1:243355/149‑1 (MQ=255)
                                                                                                                                aTTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCGTCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTAtctc                <  1:164599/149‑1 (MQ=255)
                                                                                                                                 ttccccGTGCCGGGCAGCAGGGGGCGGGGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATctct               <  2:838359/145‑1 (MQ=255)
                                                                                                                                  tATCGGGGCCTGGCAGCAGGGGGCGGGGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTg              <  2:465856/149‑1 (MQ=255)
                                                                                                                                  tATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTg              <  1:680768/149‑1 (MQ=255)
                                                                                                                                  tATCCGTGCCTGGCAGCAGGGGGCGGAGCAAGTTGGCCTCGGTAGCTGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGTATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTg              <  2:391766/149‑1 (MQ=255)
                                                                                                                                    tCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGt            <  1:911398/149‑1 (MQ=255)
                                                                                                                                          cgtGGGAGCAGGGGGCGGAGCAATTTGGCATCGGTATCGGCGGCGCCGGCCGCGTCAGCGGTTACAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTcgcc      <  2:403746/147‑1 (MQ=255)
                                                                                                                                           cTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGGTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGGTTATCCCCGGTTTcgccg     >  2:1116373/1‑149 (MQ=255)
                                                                                                                                              gcagcaGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTa  >  1:668714/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTA  >  NC_000913/810424‑810714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑