Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 246,771 G→A 5.1% P20P (CCG→CCA yafL → putative lipoprotein and C40 family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913246,7710GA 5.1% 231.8 / 2.3 78P20P (CCG→CCAyafLputative lipoprotein and C40 family peptidase
Reads supporting (aligned to +/- strand):  ref base G (32/42);  new base A (2/2);  total (34/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

TAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAA  >  NC_000913/246625‑246919
                                                                                                                                                  |                                                                                                                                                    
tAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGtt                                                                                                                                                    <  2:411133/149‑1 (MQ=255)
 aaTTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGttt                                                                                                                                                   <  1:72905/149‑1 (MQ=255)
   ttttATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTttc                                                                                                                                                 >  2:506389/1‑149 (MQ=255)
   ttttATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTttc                                                                                                                                                 <  1:293016/149‑1 (MQ=255)
     ttATAATAATGGTGTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTttctt                                                                                                                                               <  1:212350/149‑1 (MQ=255)
          ataatGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCACCATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCAttt                                                                                                                                          <  2:463159/149‑1 (MQ=255)
          ataatGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCAttt                                                                                                                                          <  2:1126251/149‑1 (MQ=255)
              tGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCGTCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCa                                                                                                                                      <  1:550075/149‑1 (MQ=255)
              tGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCa                                                                                                                                      <  1:1120944/149‑1 (MQ=255)
                         gTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACcaca                                                                                                                           >  2:293843/1‑149 (MQ=255)
                           ttGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCGGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCccccaccgc                                                                                                                         >  1:223737/1‑146 (MQ=255)
                            tGTTCAGAAACGATCCGGGATACACTGCACCATCAAAGCATGACCAGCCAGTATTTAGTATGTCCTGGCCGTCGAATCCATCCTGTGTATTGTCGGGATTACTGTTGATTTGTTCGCCGTTTTCTTCATTTCCCAGCGCCACcccatca                                                                                                                        >  2:103944/1‑149 (MQ=255)
                              ttCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACcacatcaca                                                                                                                      >  1:237215/1‑149 (MQ=255)
                                 aGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACatat                                                                                                                   <  1:965502/149‑1 (MQ=255)
                                 aGAAACGAAACCGTATACACTGCAACATTAAATCATGCCCCGCCTTTATTTAGTATGTCCTTTCCGTCGATTCCTTCCTTTGCATTGTCGGTATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACatat                                                                                                                   <  2:894350/149‑1 (MQ=255)
                                       gATCCGGGATACCCTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCttt                                                                                                             <  2:663879/149‑1 (MQ=255)
                                       gATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTTGGGGTTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCTCATATCTCttt                                                                                                             >  2:195149/1‑149 (MQ=255)
                                       gATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCttt                                                                                                             <  2:730058/149‑1 (MQ=255)
                                        gtCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTc                                                                                                            <  2:17546/148‑1 (MQ=255)
                                        aTCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTc                                                                                                            <  1:347308/149‑1 (MQ=255)
                                           cGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCATTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGc                                                                                                         >  2:1095644/1‑149 (MQ=255)
                                           cGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCATTTTCTTCATTTGCCAGCGCCACCACATCACATAACTCttttatc                                                                                                         >  2:1095679/1‑145 (MQ=255)
                                             ggATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCCCCACATCCCTTATCTCTTTCAGCTa                                                                                                       >  1:874919/1‑149 (MQ=255)
                                             ggATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTa                                                                                                       <  1:665574/149‑1 (MQ=255)
                                                tACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCCCATCACATATCTCTTTCAGCTAcgc                                                                                                    <  1:573094/149‑1 (MQ=255)
                                                   acTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTAcgccgc                                                                                                 >  1:1028557/1‑149 (MQ=255)
                                                   acTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGGAGTGGCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTAcgccgc                                                                                                 >  2:293658/1‑149 (MQ=255)
                                                     tGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGccgccc                                                                                               >  1:449547/1‑149 (MQ=255)
                                                      gCAACATTACAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGccgcccg                                                                                              <  2:496318/149‑1 (MQ=255)
                                                            ttAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCCCATATCTCTTTCAGCTACGACGCCCGCCAGCg                                                                                        >  1:1107405/1‑149 (MQ=255)
                                                            ttAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCg                                                                                        >  1:1107238/1‑149 (MQ=255)
                                                               aaGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGAt                                                                                     <  2:968157/149‑1 (MQ=255)
                                                                 gCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGc                                                                                   >  2:811498/1‑149 (MQ=255)
                                                                     gACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCaaaa                                                                               <  2:1098514/149‑1 (MQ=255)
                                                                         tgCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGt                                                                           <  2:988742/148‑1 (MQ=255)
                                                                         aGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTTCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGt                                                                           <  1:506389/149‑1 (MQ=255)
                                                                         aGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGt                                                                           >  1:1005349/1‑149 (MQ=255)
                                                                           ccATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCATTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTgc                                                                         <  1:1095644/149‑1 (MQ=255)
                                                                           ccATTATTGAGTATGGCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCATTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTgc                                                                         <  1:1095679/149‑1 (MQ=255)
                                                                             attattGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATGGTCGGGATTACTGTTGAGTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTgcgc                                                                       >  2:659143/1‑149 (MQ=255)
                                                                               tattGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGt                                                                     <  1:930530/149‑1 (MQ=255)
                                                                                 ttGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGttt                                                                   >  1:819907/1‑149 (MQ=255)
                                                                                 ttGAGTATGTCCTTGCCGTCGATGCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGttt                                                                   <  1:331402/149‑1 (MQ=255)
                                                                                    aggaTGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTttatt                                                                >  2:876596/4‑149 (MQ=255)
                                                                                        gttCCTTGCCGTCGATTCCATCCTTTGTATTGATGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCTCATATCTCTTGCAGCGACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTaaaa                                                            <  2:389089/147‑1 (MQ=255)
                                                                                         gTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAAc                                                           <  1:178561/149‑1 (MQ=255)
                                                                                         gTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCACCGGATGCAAAACCGTGCGCGTTTATTaaaag                                                           <  2:842630/149‑2 (MQ=255)
                                                                                          tCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACa                                                          <  2:465118/149‑1 (MQ=255)
                                                                                          cccTTGCCGTCGTTTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACa                                                          <  2:465218/148‑1 (MQ=255)
                                                                                            cTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTaaacccgt                                                        >  2:476502/1‑144 (MQ=255)
                                                                                            cTTGCCGTCGATTCCATCCATAGAAAGGTTGAGATTCCTAGTGAGGTGTTTGCCGTTTTCTTCATTTGACAGCTCCACCACGTCACATATATCTGTCAGCGACGCCGACCGCCAGCGGCTGCAGAAACGTGCACGGTTACAaaaagcat                                                        >  2:704227/1‑145 (MQ=255)
                                                                                               gCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTAcc                                                     <  1:664950/149‑1 (MQ=255)
                                                                                                     gATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGCTGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTc                                               >  2:918716/1‑149 (MQ=255)
                                                                                                     gATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTc                                               >  2:258351/1‑149 (MQ=255)
                                                                                                        tCCATCCTTTGTAGTGTCGGGGTTACTGTTTATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCGCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATc                                            <  2:1002120/149‑1 (MQ=255)
                                                                                                        tCCATCCCTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATc                                            <  1:181771/149‑1 (MQ=255)
                                                                                                           aTCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCAGCTGa                                         >  1:884652/1‑149 (MQ=255)
                                                                                                            tCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaa                                        >  1:988005/1‑149 (MQ=255)
                                                                                                             ccTTTGTATTGTCGGAATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACGTCACATATCTCTTTCAGCTACGCCGCCCGCCAACGGATGCAAAACCGTGCGCGTTGATTAAAACAGTACCAAACTCAGCTGaaa                                       >  2:727076/1‑149 (MQ=255)
                                                                                                                  gTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCa                                  <  2:629899/149‑1 (MQ=255)
                                                                                                                   caTTGGCGGGTTCACGGGTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAg                                 <  1:489287/148‑1 (MQ=255)
                                                                                                                       gTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCa                             <  2:1028557/149‑1 (MQ=255)
                                                                                                                        tCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGGAAAAACAGGCCAg                            >  2:461030/1‑149 (MQ=255)
                                                                                                                                aCTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCACACCCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGACAAAGCAGGCCAGCCATATtg                    >  1:267874/1‑149 (MQ=255)
                                                                                                                                  tGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATtgtg                  <  2:1005349/149‑1 (MQ=255)
                                                                                                                                  tGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATtgtg                  >  2:1119817/1‑149 (MQ=255)
                                                                                                                                    ttGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCGGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGa                >  1:620419/1‑149 (MQ=255)
                                                                                                                                    ttGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCACGCAAGCTATATTGTGGa                >  2:1137059/1‑149 (MQ=255)
                                                                                                                                     tGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGaa               <  1:659143/149‑1 (MQ=255)
                                                                                                                                     tGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGaa               <  2:171810/149‑1 (MQ=255)
                                                                                                                                     gtaTTTGTTTGCCGTTTGCTTCATTTGCCAGCGCCACCACATCACTTATCTCTTTCAGCTTCGCCGCCCGACAGCGGGGGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGaa               <  2:492747/147‑1 (MQ=255)
                                                                                                                                       aTTTGTCTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAgg             <  2:237215/149‑1 (MQ=255)
                                                                                                                                          tgtttgCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCaa          <  1:355765/149‑1 (MQ=255)
                                                                                                                                            tttgCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCAAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATTTGAAAAAGCCGGCCAGCAATTTTGTGGCAGGCAATg        >  2:389783/1‑149 (MQ=255)
                                                                                                                                            tttgCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCCACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATg        <  2:134171/149‑1 (MQ=255)
                                                                                                                                            tgtttcGTTTTCTTCAGGTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATg        <  1:798400/144‑1 (MQ=255)
                                                                                                                                               gCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCg     >  1:885269/1‑149 (MQ=255)
                                                                                                                                                ccGTTTTCTTCATTTGCCAGCGGCACCACATCACATATCTCTTTCAGCTACGCCGCCGGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGGCAGCTATATTGTGGAAGGAAATGCCgg    >  1:864189/1‑148 (MQ=255)
                                                                                                                                                  gTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAACGCAGGCCAGCTATATTGTGGAAGGCAATGCCGaaa  >  1:604641/1‑149 (MQ=255)
                                                                                                                                                  gTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGaaa  <  1:630604/149‑1 (MQ=255)
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TAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAA  >  NC_000913/246625‑246919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: