Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 151,519 A→C 6.6% I27M (ATT→ATG yadK ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913151,5190AC 6.6% 211.5 / 6.6 77I27M (ATT→ATGyadKputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base A (37/34);  new base C (3/2);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

CCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACT  >  NC_000913/151373‑151666
                                                                                                                                                  |                                                                                                                                                   
ccACCTGGTTGGTAGCTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAAt                                                                                                                                                   <  2:119264/149‑1 (MQ=255)
 cACGTGGTTGAGAACTTTCAGGAGTAACATGACCTGAAACATATCCCACCCCAACGACGCCAAGATCAATAGTATCCTGGTTACTGATTCCGCTTTTGCAGGGGCTGTTTTGTACATTGGCAACAAGGGCAATATCCTGTCCAGCAATg                                                                                                                                                  <  2:486503/149‑1 (MQ=255)
 cACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCCGCAATg                                                                                                                                                  >  2:906124/1‑149 (MQ=255)
 cACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATg                                                                                                                                                  <  1:162889/149‑1 (MQ=255)
    cTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCt                                                                                                                                               <  1:631276/149‑1 (MQ=255)
    cTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCt                                                                                                                                               <  1:160428/149‑1 (MQ=255)
      ggTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTgg                                                                                                                                             <  2:777052/149‑1 (MQ=255)
        ttGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGAc                                                                                                                                           >  1:981803/1‑149 (MQ=255)
          gATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAg                                                                                                                                         >  2:875792/1‑149 (MQ=255)
              acgttCAGGGGGAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGa                                                                                                                                     <  2:594745/146‑1 (MQ=255)
              aCTTTCAGGACTAACATTACCTGAAAAATATCCCACCCCAACGTCGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGACAggata                                                                                                                                     <  2:931192/149‑4 (MQ=255)
                tttCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGTTTGCTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCGGCAATGGCTGGACAGGGGATg                                                                                                                                   >  2:500785/1‑149 (MQ=255)
                tttCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCATCAAGGTCAATATCCTGTCCAGCACGGGCTGGGCAGGCGATg                                                                                                                                   >  1:1102204/1‑149 (MQ=255)
                tttCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATg                                                                                                                                   >  1:788674/1‑149 (MQ=255)
                      gAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGGCAGGCGATGCAAAAc                                                                                                                             >  1:346686/1‑149 (MQ=255)
                        gTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGACCAGCAATGGCTGGCCAGGCGATGCAAAACAg                                                                                                                           >  1:784534/1‑149 (MQ=255)
                         tAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGAAATGGCTGGACAGGGGATGCacaacact                                                                                                                          >  1:1119993/1‑147 (MQ=255)
                           aCATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTaa                                                                                                                        >  2:877221/1‑149 (MQ=255)
                            cATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGACAgga                                                                                                                                       <  2:464796/133‑2 (MQ=255)
                            cATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGACAgga                                                                                                                                       >  1:464796/1‑132 (MQ=255)
                                   tGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTATTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaa                                                                                                                >  2:948919/1‑149 (MQ=255)
                                     aaaaaTATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTGGCAGGTGCTGTTTTTTACATTGGCAACCAGGTCAATATCCTGTCCAGCAATGACTGGCCAGGCGATGCAACACAGTCATCacagaaac                                                                                                              >  2:421903/1‑149 (MQ=255)
                                      aaaaTATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaaca                                                                                                             <  2:1020662/149‑1 (MQ=255)
                                       aaaTAGCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACAGTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaacag                                                                                                            <  2:130439/149‑1 (MQ=255)
                                          tatCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGATGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAat                                                                                                         >  2:345523/1‑149 (MQ=255)
                                           atCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAatt                                                                                                        <  1:520614/149‑1 (MQ=255)
                                              ccACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAattatt                                                                                                     <  1:408641/149‑1 (MQ=255)
                                               cACCCCAACGCCCCCCAGACCAATATTACCCTGGATACTGACCCCCCTTTTGCAGGCGCTGATTTTTACATCGGCAACAAGGTCAATATCCTGTCCAGCAACGGCTGGAAACGCGATTCCAAACAGCACCGACAGAAACAGAatgctcc                                                                                                    >  2:537654/1‑144 (MQ=255)
                                                 ccccAACGCCGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATtctc                                                                                                  <  1:525055/149‑1 (MQ=255)
                                                 ccccAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCCGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATtctc                                                                                                  <  1:387920/149‑1 (MQ=255)
                                                  cccAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTctct                                                                                                 >  1:930890/1‑149 (MQ=255)
                                                       cggcgCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcatc                                                                                            <  2:243993/146‑1 (MQ=255)
                                                        gacgCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcatca                                                                                           >  1:20659/1‑149 (MQ=255)
                                                           gCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAACCAGTAATGACAGAAACAGAATTATTCTCTTCATCCGCt                                                                                        >  1:468577/1‑149 (MQ=255)
                                                            ccAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCtt                                                                                       <  2:597524/149‑1 (MQ=255)
                                                             cAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTa                                                                                      <  1:181421/149‑1 (MQ=255)
                                                               aagtCAATATGACCCTGGTTACTGCTTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACg                                                                                    <  1:339895/146‑1 (MQ=255)
                                                               aGATCAATATTACCCTTGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACg                                                                                    <  2:499490/149‑1 (MQ=255)
                                                                gATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGt                                                                                   >  2:17957/1‑149 (MQ=255)
                                                                 aTCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGtt                                                                                  >  2:569244/1‑149 (MQ=255)
                                                                   cAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGa                                                                                >  2:658573/1‑149 (MQ=255)
                                                                     atatTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGCGt                                                                              >  1:594685/1‑149 (MQ=255)
                                                                         tACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTAGTCTCTTCATCAGCTTACGTTGAGTGGGa                                                                          <  1:614477/149‑1 (MQ=255)
                                                                          aCCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGAt                                                                         >  1:997600/1‑149 (MQ=255)
                                                                            ccTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGc                                                                       >  1:897485/1‑149 (MQ=255)
                                                                            ccTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGc                                                                       <  1:470277/149‑1 (MQ=255)
                                                                              tGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCat                                                                     >  2:90209/1‑149 (MQ=255)
                                                                                 ttACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGCTGCatata                                                                  >  1:952926/1‑149 (MQ=255)
                                                                                    cTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAg                                                               >  1:62626/1‑149 (MQ=255)
                                                                                    cTGATTCCGCTTTTGCAGGTACTGTTTTTTACATTGACAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACGGGCGATGCAAAAAAGTAATGACAGAAACAGAATTATTCCCTTCATCAGCTTACCTTCAGGTGGATGCACATATAg                                                               >  2:961803/1‑149 (MQ=255)
                                                                                      gATTCCTGTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGtt                                                             <  1:698955/149‑1 (MQ=255)
                                                                                        ttCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTcc                                                           >  1:140087/1‑149 (MQ=255)
                                                                                          ccGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTg                                                         <  1:569244/149‑1 (MQ=255)
                                                                                                ttGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTg                                                   >  1:567342/1‑149 (MQ=255)
                                                                                                 tGCAGGTGCTGTTTTTTACATTGGCAACAATGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGt                                                  <  2:680022/149‑1 (MQ=255)
                                                                                                     ggTGCTGTTTTTTACATTGGCAAAAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGt                                              >  2:536878/1‑149 (MQ=255)
                                                                                                           gTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgtta                                        <  1:17957/149‑1 (MQ=255)
                                                                                                            ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCCGATATAGTTTCTGAATCTTTAAGTCTgttag                                       >  1:23053/1‑149 (MQ=255)
                                                                                                            ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgttag                                       >  1:1095245/1‑149 (MQ=255)
                                                                                                            ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgttag                                       >  1:784173/1‑149 (MQ=255)
                                                                                                            ttttttACATTGGAAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGAAGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGCATGCATATATAGTTCCTGAATCTGTAAGTCGgttag                                       >  1:813619/1‑149 (MQ=255)
                                                                                                                 tACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTtata                                  <  1:531473/149‑1 (MQ=255)
                                                                                                                      tGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTa                             <  2:897485/149‑1 (MQ=255)
                                                                                                                             aaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTaaa                      <  1:658573/149‑1 (MQ=255)
                                                                                                                              tgatCAAAATCCACTCCAGAAATGGCTAGACAGGCCATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAAt                     <  1:51607/146‑1 (MQ=255)
                                                                                                                              aGGTCAATATCCTGTCCAGCGATGGGTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCCTCAACTTACTTTGAGTGGGATGCATATATAGGCCCGGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAAt                     <  1:505931/149‑1 (MQ=255)
                                                                                                                                     tatCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTg              >  1:1027491/1‑149 (MQ=255)
                                                                                                                                         cTGTCCAGCCATTGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATTTGGCTGGttc          >  1:1031400/1‑147 (MQ=255)
                                                                                                                                          tGTCCAGCCATTGCTAGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCa         >  2:726931/1‑149 (MQ=255)
                                                                                                                                            tCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGa       >  2:380232/1‑149 (MQ=255)
                                                                                                                                              cAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTCGTTATAGGTTACTGTAAAGGTGGCTGGTGCAGaaa     >  2:132073/1‑149 (MQ=255)
                                                                                                                                               aGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACCGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGaaaa    <  2:897616/149‑1 (MQ=255)
                                                                                                                                               aGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGaaaa    <  1:479174/149‑1 (MQ=255)
                                                                                                                                               aGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGaaaa    <  1:323311/149‑1 (MQ=255)
                                                                                                                                               aGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGaaaa    <  2:358404/149‑1 (MQ=255)
                                                                                                                                               aGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGaaaa    <  2:140087/149‑1 (MQ=255)
                                                                                                                                                 cAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACt  <  1:90209/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACT  >  NC_000913/151373‑151666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: