Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,307,295 A→T 7.5% L169Q (CTG→CAG)  alsK ← D‑allose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,307,2950AT 7.5% 171.3 / 7.9 69L169Q (CTG→CAG) alsKD‑allose kinase
Reads supporting (aligned to +/- strand):  ref base A (27/35);  new base T (3/2);  total (31/38)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-02

GAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAG  >  NC_000913/4307147‑4307443
                                                                                                                                                    |                                                                                                                                                    
gAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTTTTTGCTCATATCTCCCa                                                                                                                                                      <  2:996370/149‑1 (MQ=255)
gAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCCCAGTGTTGGGTCATAACTCCCa                                                                                                                                                      >  2:122626/1‑149 (MQ=255)
   aGGGGCCGTTCCCGCCTGGACGAAAAGCCGCCGCAAGTTGTTATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAggg                                                                                                                                                   <  1:499785/149‑1 (MQ=255)
    ggggggTTTTCCGCATGTACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTCGTTCGTACCAACGTCTTAGCGCTATTCCTGATCAATTCGTTTGCAGGTAGCCAGGAGTGCCACACGCGCAGTGTTGGGTCATATCTCCCAgggg                                                                                                                                                  <  1:231355/149‑1 (MQ=255)
    ggggCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTCCCAGCGTCTTAGCGCCATGCCAGGGCAATTGTTTGCCAGGCACGCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAgggg                                                                                                                                                  <  1:231360/149‑1 (MQ=255)
    ggggCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAgggg                                                                                                                                                  <  1:482144/149‑1 (MQ=255)
     gggCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAGTTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGGGTTGGGTCATATGCCCCCggggg                                                                                                                                                 >  2:115045/1‑149 (MQ=255)
      ggCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGa                                                                                                                                                >  1:866922/1‑149 (MQ=255)
       gCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGTCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCAAGGATTGCCACACGCGCAGGGTTGGGTCATATCTCCCAGGGGGat                                                                                                                                               >  1:353198/1‑149 (MQ=255)
        cGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAggggggtg                                                                                                                                              >  1:911593/1‑146 (MQ=255)
         gTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATCCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGatat                                                                                                                                             <  2:347063/149‑1 (MQ=255)
           tttCCGCATGGACGAAAAGATCGCGTAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGa                                                                                                                                           <  2:495356/149‑1 (MQ=255)
           tttCCGCATGGACGAAAAGATCGCGCAATGGGTACTTTCGAGGCAGTTATAAACCCAGGTAGCTTATCACCATTCCAGAGCATTTAAGTTCCAGCCACCCATGATTGCCACACGCGCAGTGCTGGGTCATATCCCCCTGGGAGATATGa                                                                                                                                           <  2:1104036/149‑1 (MQ=255)
             tCCGCTTGGACGCAACGTCCGCGCAATGTGTAATTTCTGGGCTGTTGCTCGGACCAGCTTCTAAGCGCCAGGCCAAACCTATTGGTATCCATCCACCCAAGATTGCCACTCGCGCGGTGTTTGGTCATATCTCCCAGGGGGATATGccc                                                                                                                                         <  2:184358/149‑1 (MQ=255)
             cccGCATCGACGAAAAGTCCCCGCAATGGGTAATTTCGTGGTCGTTGTTCGTCCCGGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATGGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGAcc                                                                                                                                         <  2:787015/148‑1 (MQ=255)
                   tGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTc                                                                                                                                   <  2:461014/149‑1 (MQ=255)
                       cGAAAAGATCGCGCAAGGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCt                                                                                                                               <  2:500255/149‑1 (MQ=255)
                        gAAAAGAGCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATGGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTg                                                                                                                              >  2:798269/1‑149 (MQ=255)
                          aaaGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGGGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGcc                                                                                                                            >  1:863374/1‑149 (MQ=255)
                           aaGCTCGCGCAGTGGGTAATTTCGGGGCTGTCGTTCGGACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCca                                                                                                                           <  1:888231/149‑1 (MQ=255)
                             gATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCcaca                                                                                                                         <  2:194552/149‑1 (MQ=255)
                             gATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCcaca                                                                                                                         >  1:437857/1‑149 (MQ=255)
                              aTCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGGTATGACCCAGTTCGCCTGCCacac                                                                                                                        >  1:651578/1‑149 (MQ=255)
                                cgcgCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACCCCg                                                                                                                      >  1:727528/1‑149 (MQ=255)
                                       tGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCAc                                                                                                               <  2:147743/149‑1 (MQ=255)
                                         ggTAATTCCGGGACTGTTGTCCGTACCAGCGTCTTGGAGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGAGCCAGTTCGCCTGCCACACCGTGTGCAccc                                                                                                             <  2:40671/149‑1 (MQ=255)
                                          gTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACCCGCGCAGTGTTGGGTCGTATCCCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCg                                                                                                            >  2:261811/1‑149 (MQ=255)
                                               ttCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGGCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCAc                                                                                                       >  1:953704/1‑149 (MQ=255)
                                                        gttgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGACACACCGTGTGCACCCGTCCACGGCGCACCg                                                                                              >  2:782420/1‑149 (MQ=255)
                                                        ggtgttCGTACCAGCGTCTCACGGCCATTCCATAGCAATTGGTTTCCAGGCTCCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGAGATGACCCAGTTCGCCTGCAACACCGTGTGCACCCGTCCACGGCGCACCg                                                                                              <  2:252593/147‑1 (MQ=255)
                                                              cGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGAGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCGGTCCACGGCGCACCGTGCATc                                                                                        >  1:768986/1‑149 (MQ=255)
                                                              cGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGCTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGTACCCGTCCACGGCGCACCGTTCATc                                                                                        <  1:643276/149‑1 (MQ=255)
                                                                          ttAGCGCCATTCCAGGGCAATTGGTTTCCAGGCCCCCAGGCTTGCCAGACGCGCAGTGTTGTGTTATACCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGGCCACGGCGCACCGTTCATCCACACTGCGAAc                                                                            <  2:500905/149‑1 (MQ=255)
                                                                          tgaGCGCCATTCCACCGCAATTGGTTTCCAGGCACCGAGGAGTGCCCCACGCGCAGTGTTGGGTCATACCTCCCAGTGGGACATGACCCAGTCCCCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAc                                                                            <  2:467271/147‑1 (MQ=255)
                                                                           caGCGCCAGTCCAGAGCAATTGGTTTCCAGGCCCCCAGCATTGCCACACGCGCAGTGTTGGCTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCCTCCACGGCGCACCGTCCACCCACACTGCGAAcc                                                                           <  2:483879/148‑1 (MQ=255)
                                                                            agggcCATTCCACGGCAATGGGTGTCCAGGCACCCAGGTTTGCCACACGCGCAGCGTTGGGTCATATCTCCCAGGGTGATATGCCCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAccc                                                                          <  1:626570/146‑1 (MQ=255)
                                                                            aGCGCCATTCCCGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGCCCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAccc                                                                          <  1:429489/149‑1 (MQ=255)
                                                                                    gccggggCAATTGGTTTCCAGGCCCCCAGGATTGCCACAGGGGCAGTGTTGGGTCATATCTCCCAGGGGCATATGCCCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCg                                                                  <  2:542536/143‑1 (MQ=255)
                                                                                     ccAGAGCAATTGGTTTCCAGGCACCGAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGt                                                                 >  1:687326/1‑149 (MQ=255)
                                                                                           cgaTTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCgag                                                           <  1:630495/147‑1 (MQ=255)
                                                                                           cAATTGGTTTCCAGGCACTCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCAAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCCCGGCGCACCGTTCATCCACACTCCGAACCCCATCCCCGTACCgag                                                           >  2:741534/1‑149 (MQ=255)
                                                                                            aaTTGGTTTCCAGGCACCCAGGCTTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGCCCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCgaga                                                          <  1:1005346/149‑1 (MQ=255)
                                                                                             aTTCGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGAGATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTCCCGAGAt                                                         >  2:104445/1‑149 (MQ=255)
                                                                                              ttGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATa                                                        <  1:841302/149‑1 (MQ=255)
                                                                                                     ccAGGCACCCAGGATTGCCACACACGCAATGTTGCGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGgccgcc                                                 >  1:634130/1‑149 (MQ=255)
                                                                                                      cAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGACAGGCCGCCa                                                >  1:454375/1‑149 (MQ=255)
                                                                                                       aGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAg                                               >  1:938254/1‑149 (MQ=255)
                                                                                                          cccctatgATCGCCACACGCGCAGTTTGGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCAGCCCCGTACCGAGATAGGCCGCCAGAAc                                            <  2:547896/142‑1 (MQ=255)
                                                                                                               aGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGGTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCGGAACCAgtt                                       >  1:865611/1‑149 (MQ=255)
                                                                                                                  atggCCACACGCGCAGGTTTGGGTCATCTCTCCCGGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTGCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAgttgtt                                    <  2:1116790/146‑1 (MQ=255)
                                                                                                                  aTTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAgttgtt                                    <  1:105268/149‑1 (MQ=255)
                                                                                                                      ccACACGCTCAGGGTGGGGTCATATCGCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGt                                <  1:245375/149‑1 (MQ=255)
                                                                                                                      acacgCGCGCAGTGTTGGTTCATACCTCCCAGGGGGATATGTCCCAGTTCGCGTGCCACATCGTGTGCACCCCTCCACGGCGGACCGTTCATGCACACAGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGt                                <  2:520977/148‑1 (MQ=255)
                                                                                                                       cacaCGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCGGTACCGGGATAGGCGGCCAGAACCAGTTGTTGCgga                               >  1:265038/1‑147 (MQ=255)
                                                                                                                            gcgcAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACAGTGTGCACCCGTCCACGGCACACCGTTCATCCCCACTGCGAACCCCATCCCCGTACCGAGCTAGACACCCAGAACCAGTTGTGGCGTATGGCg                          >  2:881632/1‑149 (MQ=255)
                                                                                                                             cgcAGGTTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGGGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCgg                         <  2:1047258/149‑1 (MQ=255)
                                                                                                                               cAGTGGTGGGTCATATCTCCCAGGGGGATATCGCCCAGTTCGCCTGCCACACCGTGTGCCCCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGtt                       <  1:255738/149‑1 (MQ=255)
                                                                                                                                 gtgtTGGGTGATCCTCCCCAGGGGGATATGCCCCAGTTCGCCTGCCACCCCGTCTGCACCCGTCCACGGCGCACCGTTCATCCACACTGAGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTTAGGCGGtttt                     <  2:852319/149‑1 (MQ=255)
                                                                                                                                  tgtTGGGTCATATCGCCCAGGGGGATATGCCCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTc                    <  1:862001/149‑1 (MQ=255)
                                                                                                                                  tgtTGGATCATCTCTCCCAGGGGGGTATGACCCAGTTGGCCGGGCGCACCGTGAGCACCCATACACGGCGCCTCGTTCAGCCACACGGCGAAGCCCATCACCGTACCGACGTAGGCCGCCAGAACCAGTTGTTAGGGAAGGCggatatc                    >  2:183170/1‑144 (MQ=255)
                                                                                                                                       ggTCATATCTCCCAGGGGGATGTGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGGACCAGTTGTTGCGTAAGGCGGTTTTctacta               >  1:601279/1‑149 (MQ=255)
                                                                                                                                         tCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGACCCAGTTGTTGCGTAAGGCGGTTTTCTACTACg             >  1:881366/1‑149 (MQ=255)
                                                                                                                                         tCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTCCg             >  2:845889/1‑149 (MQ=255)
                                                                                                                                         tCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACg             >  1:725968/1‑149 (MQ=255)
                                                                                                                                         tCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACg             >  1:725952/1‑149 (MQ=255)
                                                                                                                                             atCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTccc         >  2:1144193/1‑149 (MQ=255)
                                                                                                                                              tctcCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCa        >  1:818366/1‑149 (MQ=255)
                                                                                                                                              tccccccGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCa        <  1:405841/146‑1 (MQ=255)
                                                                                                                                               ctcCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATGGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCGCAg       >  2:470585/1‑149 (MQ=255)
                                                                                                                                                 cccAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGga     <  2:938254/149‑1 (MQ=255)
                                                                                                                                                   cAGGGGGATTTGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTCCCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGgaga   <  2:866922/149‑1 (MQ=255)
                                                                                                                                                   cAGGGGGATATGTCCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGgaga   <  1:122626/149‑1 (MQ=255)
                                                                                                                                                    aGGGGGGTATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGagag  <  2:437857/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAG  >  NC_000913/4307147‑4307443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑