Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,156,588	C→A	7.1%	intergenic (+72/‑33)	yqhD → / → dkgA	aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,156,588	0	C	A	7.1%	199.7 / 6.1	71	intergenic (+72/‑33)	yqhD/dkgA	aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
Reads supporting (aligned to +/- strand):  ref base C (29/37);  new base A (2/3);  total (31/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.63e-01

ACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTT  >  NC_000913/3156440‑3156733                                                                                                                                                     |                                                                                                                                                   aCGGCATGACCCAACCGGGCGAAAATCCTGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTGCGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGcc                                                                                                                                                    <  2:388974/149‑1 (MQ=255)         aCCCAACAGGGCGAAAATCATGAGATTACGTTGGATGTCAGCCGCCGTATATACGAGGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACCCCTCACCGGAGCCTGCTCCgg                                                                                                                                            >  2:785014/1‑149 (MQ=255)           ccAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTg                                                                                                                                          >  1:879822/1‑149 (MQ=255)            ctacctggCGAAAATCATGACATTACGTTGGAGGTCAGGCGCCGTACATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACGTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCCCCGGAGTAGGCGCCGGTGa                                                                                                                                         <  2:139032/143‑1 (MQ=255)            cAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGa                                                                                                                                         <  2:865732/149‑1 (MQ=255)                ggcgCGAAAAGCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTc                                                                                                                                     <  2:222563/146‑1 (MQ=255)                 gggCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACA‑CTCACCGGAGCAGGCTCCGGTGAggtgtt                                                                                                                                   <  2:571996/149‑6 (MQ=255)                  ggCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACTCCTGCTCGCCGGAGCCTGCTCCGGTGAGTTCat                                                                                                                                   <  1:1141108/149‑1 (MQ=255)                    cGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCTTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCagat                                                                                                                                 >  2:283902/1‑149 (MQ=255)                    cGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATAAGAAACTGCCCGCTGAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCACTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCatat                                                                                                                                 >  2:759347/1‑149 (MQ=255)                     gAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCAtata                                                                                                                                >  2:869303/1‑149 (MQ=255)                       ccataaTGACATTACGTAGCATTTCAGCCTCCGTATATACGAACCCTCCCTCTAATCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCTGAGCCTGCTCCGGTGAGTTCATATaaa                                                                                                                              <  2:510382/144‑1 (MQ=255)                       aaaTCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATaaa                                                                                                                              <  2:639104/149‑1 (MQ=255)                          tCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGCAGCCTGCTCCGGTGAGTTCATATAAagga                                                                                                                           <  1:702605/149‑1 (MQ=255)                          gcATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagga                                                                                                                           <  2:1133767/148‑1 (MQ=255)                           cATGACATTACGTTGGATGTCAGCCTCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGGGCGTTAACATAAaggag                                                                                                                          >  1:580073/1‑149 (MQ=255)                             tGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAgggaggc                                                                                                                        >  1:106828/1‑148 (MQ=255)                              gACGTTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGaa                                                                                                                       <  1:121587/149‑1 (MQ=255)                                 aTTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGt                                                                                                                    <  1:173858/149‑1 (MQ=255)                                     cGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATgg                                                                                                                <  1:691506/149‑1 (MQ=255)                                     cGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATgg                                                                                                                <  2:1075133/149‑1 (MQ=255)                                           aTGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTATGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTaaac                                                                                                          >  2:898154/1‑147 (MQ=255)                                            tGTCATCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCACACCTTCGTTTCCGGGCAGTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATcc                                                                                                         <  2:788330/149‑1 (MQ=255)                                                 gccgccGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCg                                                                                                    >  2:131942/1‑149 (MQ=255)                                                  ccgccgTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGGCTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATAAAAAGGAGGAACGTATGGCTAATCCAACCGt                                                                                                   >  1:160750/1‑149 (MQ=255)                                                   cgccgTAGATACGAAGCCGCCCCCTAAGCCTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGtt                                                                                                  <  1:376614/149‑1 (MQ=255)                                                     ccgTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGGGTTCATATAAGGGAGGAACGTATGGCTAATCCAACCGttat                                                                                                >  2:349112/1‑149 (MQ=255)                                                      cgtttttaCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatt                                                                                               <  2:62969/143‑1 (MQ=255)                                                       ttatataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTGAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATCTCACGGAGGAACGTATGGCTAATCCAACCGtgatta                                                                                              <  2:105414/148‑1 (MQ=255)                                                        tatataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttattca                                                                                             >  1:260162/1‑147 (MQ=255)                                                        tatataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTATTCGGGCATTTCGTCCAGTCTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTaa                                                                                             >  2:944597/1‑149 (MQ=255)                                                          aataCGAAGCCGCCCGATTAGCTTTTTACGCCTACAACCTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGc                                                                                           <  2:629740/148‑1 (MQ=255)                                                           ataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCt                                                                                          <  1:491506/149‑1 (MQ=255)                                                              cGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACa                                                                                       >  1:393579/1‑149 (MQ=255)                                                                   ccgcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATg                                                                                  <  1:1075585/149‑1 (MQ=255)                                                                             aGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCAt                                                                       <  2:852767/149‑1 (MQ=255)                                                                              gCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATg                                                                      <  1:454219/149‑1 (MQ=255)                                                                              gCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCCCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATg                                                                      <  2:774768/149‑1 (MQ=255)                                                                                tttttACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCACTGTCATGcc                                                                    >  2:999792/1‑149 (MQ=255)                                                                                 ttttACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGccc                                                                   <  2:59931/149‑1 (MQ=255)                                                                                      cGCCTGAAACTTTCGTTTTCGGGCATTTCGTCTAGACTTAAGTGCACAACACCTCACCGGAGCCTGCTACGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATTTCATGCCCCAGCt                                                              <  1:785014/149‑1 (MQ=255)                                                                                       gCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTg                                                             <  1:708884/149‑1 (MQ=255)                                                                                        ccTCAAGCTTTCGTTTTCGGGCATTTCGCCCAGACCTAAGTTCACAACACCTCACCGGGGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTgg                                                            <  2:268622/149‑1 (MQ=255)                                                                                        ccTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTgg                                                            <  2:114756/149‑1 (MQ=255)                                                                                         cTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTggg                                                           >  1:451108/1‑149 (MQ=255)                                                                                          tCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGa                                                          >  2:581806/1‑149 (MQ=255)                                                                                          tCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCAAATAAGGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAAAGTCATGCCCCAGCTGGGa                                                          >  1:326809/1‑149 (MQ=255)                                                                                          tCAAACTTTCGGTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATACAGGAGGGACGTATGGCGAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGa                                                          <  2:418750/149‑1 (MQ=255)                                                                                           cAAACTTTCGTTCTCGGGCATTTCGTCCAGACTTAAGTGCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTACGCTACAGGATG‑GCAATGTCATGCCCCAGCTgggcc                                                         >  1:166935/1‑147 (MQ=255)                                                                                             aaCTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCCTATACCGGAGGAACGCATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTg                                                       <  1:447457/149‑1 (MQ=255)                                                                                              aCTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCTCAGCTGGGACTgg                                                      <  1:785531/149‑1 (MQ=255)                                                                                                     ttttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCAAGCTGGGACTGGGCGTATg                                               >  1:459950/1‑149 (MQ=255)                                                                                                             cATTTCGTCCAGACTTCAGATCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgcaagcaa                                       <  1:22696/149‑1 (MQ=255)                                                                                                             cATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAACCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgcaagcaa                                       <  2:207438/149‑1 (MQ=255)                                                                                                                ttCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTa                                    <  1:1085175/149‑1 (MQ=255)                                                                                                                  cGTCCAGCCTTATGTTCACAACACCCCACCGGAGCCTGCTACGGTGAGTTCATATAAAGGAGGAACGTATCCGTCAATCGGTCATAATCGAGCGACAGAGTGATCGATG‑GATGCCCCAGCTAGGACTGGTCGGCTGGCAAGCAAGTAAt                                  <  2:245079/149‑1 (MQ=255)                                                                                                                    tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGa                                <  1:1068018/149‑1 (MQ=255)                                                                                                                    cccagctttAGGTTCACACGACCTCACCGGACCCTGCCCCGGTGAGTTCCTATAGAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGACC‑GCAATGTCATGCCCCCGCTGGGACTGGGTGTCTGGCAAGCAAGTAATGa                                <  2:29013/142‑1 (MQ=255)                                                                                                                      cAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGGTGTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAgg                              >  1:847925/1‑149 (MQ=255)                                                                                                                       aGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAAt                                                                            >  2:330240/1‑102 (MQ=255)                                                                                                                       aGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAAt                                                                            <  1:330240/102‑1 (MQ=255)                                                                                                                       aGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGGCTGGGCATCTGGCAAGCAAGTAAggagga                             >  1:757958/1‑149 (MQ=255)                                                                                                                           ttAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTa                         >  1:721945/1‑149 (MQ=255)                                                                                                                                 tCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTGATATAAAGGAGGAACGTATCGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCCGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCAcc                   >  1:413352/1‑149 (MQ=255)                                                                                                                                   acaacaCCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAATAATCACCGc                 >  2:162747/1‑149 (MQ=255)                                                                                                                                      acacCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCAt              >  2:890044/1‑149 (MQ=255)                                                                                                                                          cTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaa          >  1:664325/1‑149 (MQ=255)                                                                                                                                          cTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaa          >  1:664264/1‑149 (MQ=255)                                                                                                                                           tCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaaa         >  1:582828/1‑149 (MQ=255)                                                                                                                                           tCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaaa         >  2:482996/1‑149 (MQ=255)                                                                                                                                               cGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGACGTTATCACCGCCGTTCAAAAAGc     <  2:393579/149‑1 (MQ=255)                                                                                                                                                  aGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGtt  <  1:1151990/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATG‑GCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTT  >  NC_000913/3156440‑3156733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑