Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,741,292 C→A intergenic (‑144/‑66) aroF ← / → yfiL 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible/lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,741,2920CA97.5% 259.4 / ‑8.0 81intergenic (‑144/‑66)aroF/yfiL3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible/lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (52/27);  minor base T (2/0);  total (54/27)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGATGGCGATCCTGTTTATGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACCTCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCTTATACTCACGCGCC  >  NC_000913/2741146‑2741434
                                                                                                                                                  |                                                                                                                                              
cATGATGGCGATCCTGTTTATGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACatc                                                                                                                                              <  1:459921/149‑1 (MQ=255)
 atgatgGCGATCCTGTTTATGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACatct                                                                                                                                             <  2:1022570/149‑1 (MQ=255)
     tgGCGATCCTGTTTATGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGGTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGtat                                                                                                                                         <  1:175112/149‑1 (MQ=255)
                 ttATGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTac                                                                                                                             <  1:110337/149‑1 (MQ=255)
                   tttctctTTTGTGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAGTTTAGTCGCGCTTTCAACCCTTACATCTGTTTAGATATATTTacac                                                                                                                           >  2:1352698/4‑149 (MQ=255)
                    tGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACt                                                                                                                          >  1:821056/1‑149 (MQ=255)
                       tCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACCCTccc                                                                                                                       >  2:745995/1‑149 (MQ=255)
                              cGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACGTTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACTTTTGTATGGATAAATTTATATTCCGTTTGtaa                                                                                                                >  1:547747/1‑149 (MQ=255)
                              cGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGaaa                                                                                                                <  1:1417988/149‑1 (MQ=255)
                               gATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGaaaa                                                                                                               <  2:1239335/149‑1 (MQ=255)
                               gATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGaaaa                                                                                                               >  1:766269/1‑149 (MQ=255)
                               gATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGaaaa                                                                                                               <  2:821056/149‑1 (MQ=255)
                               gATAGTTGATCCTCAGCGAGGATGACGGAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGACATTTAGTCGCGCTTTCAACCCTTACATCTGTAAAGATAAATTTACACTCGCTTTGaaca                                                                                                               >  2:566233/1‑147 (MQ=255)
                                    ttGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGACAACAATc                                                                                                          >  2:490217/1‑149 (MQ=255)
                                    ttGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATc                                                                                                          >  2:343346/1‑149 (MQ=255)
                                    ttGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATc                                                                                                          >  1:53549/1‑149 (MQ=255)
                                      gctCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCg                                                                                                        <  2:173015/147‑1 (MQ=255)
                                         ccgcAGCGAGGATGACGTAACGATAACACAGAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTa                                                                                                     <  1:1322239/146‑1 (MQ=255)
                                         ccTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTa                                                                                                     >  1:880323/1‑149 (MQ=255)
                                          cTCAGCGCGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAAGCCATGTTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTCAACACAATCCCCTAt                                                                                                    >  2:317253/1‑149 (MQ=255)
                                           tCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATg                                                                                                   >  2:1510092/1‑149 (MQ=255)
                                           tCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATg                                                                                                   >  2:748300/1‑149 (MQ=255)
                                             aGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCt                                                                                                 <  2:989590/149‑1 (MQ=255)
                                              gCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAACCAATCCGCTTTGCtt                                                                                                >  1:61414/1‑149 (MQ=255)
                                               cGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCACTTTGAAAACAATCCGCTATGCttt                                                                                               <  1:870621/149‑1 (MQ=255)
                                                 aGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGa                                                                                             <  1:216113/149‑1 (MQ=255)
                                                 aGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCCCTATGCTTTGa                                                                                             >  1:1501299/1‑149 (MQ=255)
                                                     tGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGaaaaa                                                                                         <  2:1340965/149‑1 (MQ=255)
                                                      gACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAg                                                                                        >  1:299294/1‑149 (MQ=255)
                                                      gACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAg                                                                                        >  2:537378/1‑149 (MQ=255)
                                                      gACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAAGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATCAATTTACACTCCCTTTGAAAAAAATCCGCTATGCTTTGAAAAAg                                                                                        >  2:499151/1‑149 (MQ=255)
                                                       aCGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACCCTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAgg                                                                                       <  1:1249535/149‑1 (MQ=255)
                                                             cGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCACTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGtagacac                                                                                 >  1:445412/1‑146 (MQ=255)
                                                                 aaCACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGGAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAat                                                                             >  1:1547480/1‑149 (MQ=255)
                                                                    acaTAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAatgat                                                                          >  1:975801/1‑149 (MQ=255)
                                                                       taaagtaaagTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGaa                                                                       <  2:880323/149‑1 (MQ=255)
                                                                        aaagtaaagtTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGaaa                                                                      >  2:79557/1‑149 (MQ=255)
                                                                         aagtaaagtTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGaaaa                                                                     >  2:917213/1‑149 (MQ=255)
                                                                         aagtaaagtTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGaaaa                                                                     >  1:351293/1‑149 (MQ=255)
                                                                                 ttttCAATCCATATTTCGTACATTTTTATTTAAACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAACTGATGAAAAAGTTTATc                                                             >  2:449388/1‑149 (MQ=255)
                                                                                       aTCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGccccc                                                       <  1:537378/149‑1 (MQ=255)
                                                                                       aTCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGccccc                                                       >  1:1471932/1‑149 (MQ=255)
                                                                                        tCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAACTGATGAAAAAGTTTATCGCCCCCt                                                      >  1:519153/1‑149 (MQ=255)
                                                                                        tCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCt                                                      >  1:519075/1‑149 (MQ=255)
                                                                                         ccATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGGGAAAGAAATGATGAAAAAGTTTATCGCCCCCtt                                                     >  1:567812/1‑149 (MQ=255)
                                                                                         ccATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCtt                                                     >  1:268082/1‑149 (MQ=255)
                                                                                         ccATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCtt                                                     >  1:550002/1‑149 (MQ=255)
                                                                                          cATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCttg                                                    >  1:1409670/1‑149 (MQ=255)
                                                                                          cATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCttg                                                    >  2:168264/1‑149 (MQ=255)
                                                                                             atTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACAATCCCTTTGAAAACAATCCGCTATGCCTTGAAAAAGGAGAAAGAAATGACGAAAAAGTTTATCGCCCCCttgttg                                                 >  2:444498/1‑149 (MQ=255)
                                                                                                 cGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCtt                                             <  2:1062974/149‑1 (MQ=255)
                                                                                                  gTACATTTTTATTTACGCAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCttt                                            >  2:1371317/1‑149 (MQ=255)
                                                                                                  gTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCttt                                            >  2:556391/1‑149 (MQ=255)
                                                                                                    aCATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAATAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTAc                                          <  2:872467/149‑1 (MQ=255)
                                                                                                    aCATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTAc                                          <  1:267732/149‑1 (MQ=255)
                                                                                                    aCATTTTTATTTACACAGGCAAATTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTAc                                          >  2:218075/1‑149 (MQ=255)
                                                                                                      ctttttATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGAAAAAAAATAAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTg                                        <  2:765269/148‑1 (MQ=255)
                                                                                                      aTTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTg                                        >  1:1225247/1‑149 (MQ=255)
                                                                                                       tttttATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCGTTGTTGGCTTTACTgg                                       >  2:464471/1‑149 (MQ=255)
                                                                                                        ttttATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTATGAAAAAGGCGAAAGAAATGATGAAAAGGATTATCGCCCCCTTGTTGGCTTTatcggt                                      >  1:739485/1‑144 (MQ=255)
                                                                                                          ttatttaCACAGGCAATTCAGTCGCGCTTTCAACCCTTACATCTGTATAGAGAAAGTTCGACTCCCTTTGAACACAATCCGCTATGCTTTTAAAAAGGAGAAAGAAATGATGAAAAAGGTTATCGCCCCCTTGTTGGCTTTACTGGTTa                                    <  1:805958/149‑1 (MQ=255)
                                                                                                           tatttaCACCGGCAATTTAGTCGCGCTTTCCACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAg                                   <  1:1296481/149‑1 (MQ=255)
                                                                                                             tttaCACAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCg                                 <  2:154852/149‑1 (MQ=255)
                                                                                                             tttaCACAGGCAATTTAGTCGCGCTTTAAACCCTTACATCGGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCg                                 >  1:914322/1‑149 (MQ=255)
                                                                                                                acacAGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAATGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGAt                              >  2:59337/1‑149 (MQ=255)
                                                                                                                  acaGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGt                            >  2:1253923/1‑149 (MQ=255)
                                                                                                                  acaGGCAATTTAGTCGCGCTTTCAACCCTTACAACCGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGt                            >  2:542712/1‑149 (MQ=255)
                                                                                                                   caGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTAGCTTTACTGGTTAGCGGATGTc                           >  1:353341/1‑149 (MQ=255)
                                                                                                                    aGGCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCa                          >  1:1536223/1‑149 (MQ=255)
                                                                                                                     ggCAATTTAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCGATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGCTGGCAg                         >  1:282184/1‑149 (MQ=255)
                                                                                                                      gCAATTTAGTCGCGCTTTGAACCGTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGa                        >  2:572151/1‑149 (MQ=255)
                                                                                                                       cAATCTCGTCGCGCTTTCAACCCGTACATCTGTATAGATAAATTTACACTCCCTTTGCAAACAAACCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGAt                       <  1:1510092/149‑1 (MQ=255)
                                                                                                                        caTTTCGTCGCGCTTTCAACCCTTCCATCTGCATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGAtt                      <  1:709376/148‑1 (MQ=255)
                                                                                                                          tttAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGa                    <  2:1471932/149‑1 (MQ=255)
                                                                                                                           ttAGTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGAt                   <  2:550002/149‑1 (MQ=255)
                                                                                                                              gTCGCGCTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCt                <  2:285721/149‑1 (MQ=255)
                                                                                                                                cgcgCTTTCAGCCCTTACATCTGTATAGATAAATTTACGCTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCTta              <  2:669353/149‑1 (MQ=255)
                                                                                                                                 gcgcTTTCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCTGCTATGCTTTGAAAAAGGAGAAAGAAATGATGGAAAAGTTTATCGCCCCCTTGTTGGCGTTACTGGGTAGCGGATGTCAGATTGATCCttct             >  2:1000283/1‑147 (MQ=255)
                                                                                                                                 gcgcTTTCAACCCTTACAGCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCTGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCTtat             >  2:1000827/1‑149 (MQ=255)
                                                                                                                                       tCAACCCTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCTTTATACTCAc       >  1:122637/1‑149 (MQ=255)
                                                                                                                                            ccTTACTTCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCTTATACTCACGCGcc  >  2:1351924/1‑149 (MQ=255)
                                                                                                                                            ccTTACATCTGTCTAGATAAATTTACACTCCCATTGAAAACAATCCTCTATGCTTTGAAAGAGGAGAATGCAATGATGCACAAGTTTATCCCCCCCTTGTTGGCTTTACTGGTTCGCGGCTGCCAGATTACTCCGTATCCTCACgcgca  >  2:735452/1‑148 (MQ=255)
                                                                                                                                            ccTTACATCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCGGATTGATCCTTATACTCAcgcgac  >  1:448584/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CATGATGGCGATCCTGTTTATGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACCTCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCTTATACTCACGCGCC  >  NC_000913/2741146‑2741434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: