Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,093,040 G→A A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,093,0400GA97.6% 254.2 / NA 84A215T (GCT→ACT) hisChistidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (49/31);  minor base T (1/1);  total (51/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGT  >  NC_000913/2092896‑2093185
                                                                                                                                                |                                                                                                                                                  
gATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACttt                                                                                                                                                >  2:192794/1‑149 (MQ=255)
 aTTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGGATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACtttt                                                                                                                                               >  1:534538/1‑149 (MQ=255)
 aTTTTCGCACCCTGCTGGAGTGACCCCGCGGGAAGGCGATCGTGGTTGCCGATGAAGCCTAGCTCGAGTTTTGCCCGCAGGCATCGCTGGCTCGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACtttt                                                                                                                                               <  2:975555/149‑1 (MQ=255)
        cACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTgg                                                                                                                                        >  1:528497/1‑149 (MQ=255)
          cccTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACCTTTGCTCTGGCg                                                                                                                                      >  1:634679/1‑149 (MQ=255)
           ccTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAGTCCTGGCACCGGGCGTGGTTACGCACACTGTCGAAAACTTTTGCTCTGGcag                                                                                                                                     >  1:120404/1‑147 (MQ=255)
           ccTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGCCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGATCTGGCgg                                                                                                                                     >  2:7132/1‑149 (MQ=255)
            ctgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATGCCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCggg                                                                                                                                    <  1:1206890/149‑1 (MQ=255)
             tgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTAGTTTACGCACACTGTCGAAAGCTTTTTCTCTGGCgggg                                                                                                                                   >  2:372686/1‑149 (MQ=255)
                 ggAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTc                                                                                                                               <  1:77051/149‑1 (MQ=255)
                  gAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCGCACTGGCGAAAACTTTTGCTCTGGCGGGGCTTCg                                                                                                                              >  2:1336085/1‑149 (MQ=255)
                  gAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCg                                                                                                                              >  1:278229/1‑149 (MQ=255)
                    gTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACCCTCTCGAGAACTTTTGCTCTGGCGGGGCTTCGtt                                                                                                                            >  2:297686/1‑149 (MQ=255)
                    gTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGtt                                                                                                                            <  1:146922/149‑1 (MQ=255)
                      tAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGc                                                                                                                          <  1:875642/149‑1 (MQ=255)
                       aaCCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTTGCTGGCGGAATATCCGCACCTGGCTATTTTCCGCACAATGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCg                                                                                                                         >  1:289129/1‑149 (MQ=255)
                       aaCCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCg                                                                                                                         >  1:1495684/1‑149 (MQ=255)
                        gcccGCGGTAAGGCGATTGTGGTTGCCGAGGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCgg                                                                                                                        <  1:8584/148‑1 (MQ=255)
                        aCCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGTCGGGGCTTCGTTGCgg                                                                                                                        >  2:34483/1‑149 (MQ=255)
                          ccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCGCACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCgggt                                                                                                                      >  2:66182/1‑147 (MQ=255)
                          ccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGAt                                                                                                                      >  1:35143/1‑149 (MQ=255)
                          ccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGGTGCGGAt                                                                                                                      >  1:351571/1‑149 (MQ=255)
                              ggTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCGGGCTGTTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGGtttcg                                                                                                                  >  2:382982/1‑147 (MQ=255)
                               gTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTGTACGCAAAAGGTGGCCAACTTTTTCTCTGGCGGGGCTTCGTTGCGGATTTACg                                                                                                                 >  1:673572/1‑149 (MQ=255)
                                tAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCGGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCCTCGTTGCGGATTTACGc                                                                                                                >  2:1369323/1‑149 (MQ=255)
                                  aGGCGATTGTGCGTGCCGATGAAGCCTATATCGAGTTTTGCCCGCGGGCATCGCTGGCTTGCTGGCTGGCGGAATAGCCGCATCTGTCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTg                                                                                                              <  1:1483055/149‑1 (MQ=255)
                                    gCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCAGCGCTGGCTGGCTGGCAGGCGGAATATCCGCCCCTGGCTGTTTTACGCACACTGTCGAAAACTTTTCCTCTGGCGGGGCCTCGTTGCGGCTTTACGCTGGc                                                                                                            >  2:437420/1‑149 (MQ=255)
                                       aTTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACCTTGTATTATTCATGGGCTCTGGAGGGGGTGCGTTGCGGGTTTACGCTGGCaaa                                                                                                         >  1:1519057/1‑149 (MQ=255)
                                         tgtgGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACg                                                                                                       >  2:1074730/1‑149 (MQ=255)
                                              ttGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                                  <  2:1531927/149‑1 (MQ=255)
                                              ttGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                                  <  1:132457/149‑1 (MQ=255)
                                              ttGCCGATGAAGCCTATATCGAGTTTGGCCCCCAGGCACCGCTGGCTGGCTGGCTGCCGGAACATCCGCACCTGGCTATTTTCCGCACCCAGTCCAAAACTTTTGCTCTGGCGGGCCTTCGTTTCGGATGTACGCTGGCAAACgaagaa                                                                                                  <  2:529297/149‑1 (MQ=255)
                                                    aTGAAGCCTATATCGAGTTTGGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGtcatc                                                                                            <  2:148506/149‑1 (MQ=255)
                                                      gAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTGTTTTACGCCCACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGATGGCAAACGGAGAAGTCATCaa                                                                                          >  2:1405641/1‑149 (MQ=255)
                                                       aaGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAAc                                                                                         <  1:312725/149‑1 (MQ=255)
                                                           cTATCTCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGTGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctgc                                                                                     <  1:689632/149‑1 (MQ=255)
                                                              tatCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACAGTCGAAAACTTTTGCTGTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGGCATCAAActgctgg                                                                                  >  1:388027/1‑148 (MQ=255)
                                                                 cGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCACACGAAGAAGTCATCAACCTGCtgatga                                                                               >  2:373313/1‑149 (MQ=255)
                                                                 cGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtgatga                                                                               >  2:1315795/1‑149 (MQ=255)
                                                                     ttttGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGt                                                                           >  1:269696/1‑149 (MQ=255)
                                                                           ccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGGTTTACGCTGGCGAACGAAGAAGTCATGAACCTGCTGATGAACGTGATCGc                                                                     >  1:194006/1‑149 (MQ=255)
                                                                           ccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGc                                                                     >  2:31417/1‑149 (MQ=255)
                                                                             gCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGccc                                                                   >  1:1122121/1‑149 (MQ=255)
                                                                              cgtgCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGcccc                                                                  <  2:1536347/146‑1 (MQ=255)
                                                                               cggCATCGCTGGCTGGCGGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGccccc                                                                 <  2:215661/148‑1 (MQ=255)
                                                                                ggCATCGCCGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCt                                                                <  1:663528/149‑1 (MQ=255)
                                                                                     cGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGACGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCg                                                          >  1:171778/1‑149 (MQ=255)
                                                                                       ctggctggctggctggCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCg                                                          <  1:969493/147‑1 (MQ=255)
                                                                                       ctggctggctggctggCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCg                                                          >  2:969493/1‑147 (MQ=255)
                                                                                       ctggctggctggctggCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGct                                                        <  1:1420714/149‑1 (MQ=255)
                                                                                            tggctggctggcGGAATATCCGCACATGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGa                                                   >  1:779166/1‑149 (MQ=255)
                                                                                               ctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTcgccgc                                                >  1:1106701/1‑149 (MQ=255)
                                                                                               ctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGc                                                >  2:588723/1‑149 (MQ=255)
                                                                                                 gtcttgcGGAATAGCCGCACCTGCGTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCg                                              <  2:1126176/144‑1 (MQ=255)
                                                                                                  gctggcGGAATACCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCgg                                             <  2:1519057/149‑1 (MQ=255)
                                                                                                      gcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGc                                         >  1:308804/1‑149 (MQ=255)
                                                                                                      gcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGc                                         >  1:133996/1‑149 (MQ=255)
                                                                                                      gcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTACTAAGGCGTGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGc                                         <  1:817876/149‑1 (MQ=255)
                                                                                                       cGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGcc                                        <  2:278229/149‑1 (MQ=255)
                                                                                                        ggAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCg                                       >  1:913329/1‑149 (MQ=255)
                                                                                                         gAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTCCCCGCTCTCGACGCCGGTTGCCGa                                      <  1:1155945/149‑1 (MQ=255)
                                                                                                         gAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGa                                      <  1:1315795/149‑1 (MQ=255)
                                                                                                          aaTATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGAc                                     >  1:733544/1‑149 (MQ=255)
                                                                                                          aaTATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGAGGCTTCGATGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGAc                                     >  1:950080/1‑149 (MQ=255)
                                                                                                               ccGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGc                                >  1:897765/1‑149 (MQ=255)
                                                                                                                  cACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGc                             >  2:336539/1‑149 (MQ=255)
                                                                                                                  cACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGc                             <  1:192794/149‑1 (MQ=255)
                                                                                                                  cACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCGGATGAAAGTGATCGCCCCCTACCCGCACTCGGCGCCGGTTGCCGACATggcggc                             >  2:271380/1‑149 (MQ=255)
                                                                                                                     cTGGCTATTTTACGCACACTGTCGAAAAGTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCa                          >  1:934115/1‑149 (MQ=255)
                                                                                                                     cTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCa                          <  1:747339/149‑1 (MQ=255)
                                                                                                                     cTGGCTATCTTACGCACACTGTCGAAAACTTTTGCCCGGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCa                          <  1:25121/149‑1 (MQ=255)
                                                                                                                       ggCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGCAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGTCGCCGGTTGCCGACATTGCGGCCCAgg                        >  1:1083439/1‑149 (MQ=255)
                                                                                                                       ggCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAgg                        >  1:270886/1‑149 (MQ=255)
                                                                                                                            ttttACGCACACTGTCGAAACCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTCACAGGGTTTCGCGCCGGTTGCCGACATTGCGGCCCAAGCGTTa                   <  1:758711/149‑1 (MQ=255)
                                                                                                                             tttACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTaa                  <  1:152857/149‑1 (MQ=255)
                                                                                                                             gttACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTaa                  <  1:1469962/148‑1 (MQ=255)
                                                                                                                                  gCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCca             >  1:1212903/1‑149 (MQ=255)
                                                                                                                                  gCACACGGCCGAAATCTTTCGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCca             <  1:1336085/149‑1 (MQ=255)
                                                                                                                                    acacTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCcaca           >  2:597315/1‑149 (MQ=255)
                                                                                                                                    acacTCTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCcaca           <  2:955474/149‑1 (MQ=255)
                                                                                                                                      acTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAgg         >  1:1387721/1‑149 (MQ=255)
                                                                                                                                      acTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAgg         >  1:1387705/1‑149 (MQ=255)
                                                                                                                                       cTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCGCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAggg        <  1:185187/149‑1 (MQ=255)
                                                                                                                                          tCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAt     >  1:483289/1‑149 (MQ=255)
                                                                                                                                             aaaaCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGCCGCCGGATGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtcgt  >  1:811446/1‑149 (MQ=255)
                                                                                                                                             aaaaCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtcgt  >  1:464259/1‑149 (MQ=255)
                                                                                                                                             aaaaCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtcgt  >  2:1539665/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGT  >  NC_000913/2092896‑2093185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: