breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsGlyA_D40_I0_S2122_L001_R1_001.good.fq910,143135,569,477100.0%149.0 bases149 bases98.4%
errorsGlyA_D40_I0_S2122_L001_R2_001.good.fq910,143135,569,477100.0%149.0 bases149 bases92.2%
total1,820,286271,138,954100.0%149.0 bases149 bases95.3%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,65354.64.4100.0%Escherichia coli str. K-12 sbstr. MG1655, complete genome. Live strain from Systems Biology Research Group
total4,641,653100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000005241
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500057
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.004

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.85401

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.05
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.3.4.3
R3.5.1

Execution Times

stepstartendelapsed
Read and reference sequence file input07:40:55 30 Oct 201807:41:19 30 Oct 201824 seconds
Read alignment to reference genome07:41:20 30 Oct 201807:45:53 30 Oct 20184 minutes 33 seconds
Preprocessing alignments for candidate junction identification07:45:53 30 Oct 201807:46:14 30 Oct 201821 seconds
Preliminary analysis of coverage distribution07:46:14 30 Oct 201807:47:18 30 Oct 20181 minute 4 seconds
Identifying junction candidates07:47:18 30 Oct 201807:47:18 30 Oct 20180 seconds
Re-alignment to junction candidates07:47:18 30 Oct 201807:48:09 30 Oct 201851 seconds
Resolving best read alignments07:48:09 30 Oct 201807:48:44 30 Oct 201835 seconds
Creating BAM files07:48:44 30 Oct 201807:49:52 30 Oct 20181 minute 8 seconds
Tabulating error counts07:49:52 30 Oct 201807:50:12 30 Oct 201820 seconds
Re-calibrating base error rates07:50:12 30 Oct 201807:50:13 30 Oct 20181 second
Examining read alignment evidence07:50:13 30 Oct 201808:13:09 30 Oct 201822 minutes 56 seconds
Polymorphism statistics08:13:09 30 Oct 201808:13:09 30 Oct 20180 seconds
Output08:13:09 30 Oct 201808:13:17 30 Oct 20188 seconds
Total 32 minutes 21 seconds