Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,117,004 T→A 100% D73V (GAT→GTT)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,117,0040TA100.0% 148.5 / ‑6.7 54D73V (GAT→GTT) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (21/30);  minor base C (0/2);  total (21/33)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCA  >  NC_000913/4116862‑4117147
                                                                                                                                              |                                                                                                                                               
aCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGTGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCTTACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAAc                                                                                                                                           <  1:855515/149‑1 (MQ=255)
        gTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCg                                                                                                                                   <  1:881998/149‑1 (MQ=255)
          tttAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGc                                                                                                                                 <  1:135957/149‑1 (MQ=255)
                tGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGCGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGc                                                                                                                           <  1:610734/149‑1 (MQ=255)
                tGCTCGCAGATTTCTGCCGTACGGCGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGc                                                                                                                           <  2:488995/149‑1 (MQ=255)
                 gCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGcc                                                                                                                          <  1:202163/149‑1 (MQ=255)
                  cTCACAGACTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCa                                                                                                                         <  2:116236/149‑1 (MQ=255)
                   tCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCCCGCTGGTTCGTAATACCGATAGCTGCCATTTGAACGGAACTGATATCGGCTTTCGccgg                                                                                                                        >  1:78551/1‑147 (MQ=255)
                   tCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAACAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAg                                                                                                                        <  1:45390/149‑1 (MQ=255)
                           ttCTGCGGTACGCCGGCAATGCCAGACAATGGCGTTGTAGATTGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTa                                                                                                                <  1:192107/149‑1 (MQ=255)
                           ttCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTa                                                                                                                <  2:303375/149‑1 (MQ=255)
                               gCGGTACGATGGCACTTCCAGACAAAGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAg                                                                                                            <  1:662246/149‑1 (MQ=255)
                                  gTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTTTTTTTTTTTTGTGGTTTCACGCGGGTTCGTAATCCCGATAGCTGCAATGTGGACGGAACTGATATCGGCTCTCGCCGGCACTCCTACCAGCGt                                                                                                         >  2:710667/1‑149 (MQ=255)
                                  gTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCCGCACTTCTCCCAGCGt                                                                                                         >  2:158634/1‑149 (MQ=255)
                                  gTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGt                                                                                                         <  1:872456/149‑1 (MQ=255)
                                  gTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAAGAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCACTTTGAACGGCACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGt                                                                                                         >  2:158581/1‑149 (MQ=255)
                                   tACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATGTGAACGGAACTGATATCGGCTTTCGCCAGCGCTTCTACAGGCGTg                                                                                                        >  2:742561/1‑149 (MQ=255)
                                     cgacgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCACTTTGAACGGAACTGGTATCGGCTTTCGCCAGCACTTCTACCAGCGTGGa                                                                                                      >  1:834986/1‑149 (MQ=255)
                                       acgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTGCTTTTTCCGAGAAAATAGTGGTTTCACGCTGGTTCGTAATACGGATAGCTGCAATTTGAACGGGGCGAATATAGGATTTTACCCGCTCTTCTCCCcgcgcgtggg                                                                                                    >  2:230756/1‑143 (MQ=255)
                                         ggCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCtt                                                                                                  >  1:731326/1‑149 (MQ=255)
                                         ggCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGGAATACCGATAGCTGCAATTTGAACGGAACTGAAATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCtt                                                                                                  >  2:303349/1‑149 (MQ=255)
                                           cACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAAGAGTGGGTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTg                                                                                                >  2:536691/1‑149 (MQ=255)
                                            aCTGCCAGACAATGGCGTTATAGGTAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTgg                                                                                               <  2:196794/149‑1 (MQ=255)
                                            aCTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTgg                                                                                               <  1:591650/149‑1 (MQ=255)
                                                ccAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGATGCAATTTGACCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGTGTgg                                                                                           <  2:311644/149‑1 (MQ=255)
                                                  aGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGcg                                                                                         >  1:55116/1‑148 (MQ=255)
                                                         ggCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAttt                                                                                  <  1:500934/149‑1 (MQ=255)
                                                           cGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGGGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                                                <  2:579694/149‑1 (MQ=255)
                                                               atagctagGCTTGCCGGTTTCTTTGTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTg                                                                            <  2:681618/149‑1 (MQ=255)
                                                                  gatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCCGCATTCCATTGGGt                                                                         >  2:142504/1‑149 (MQ=255)
                                                                        cTTGCCGGTTTCTTTTTCCCGGACAATAGTGGTTTCACGCTGGTTCGTAATCCCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGtt                                                                   <  1:582677/149‑1 (MQ=255)
                                                                        cTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGtt                                                                   <  1:582709/149‑1 (MQ=255)
                                                                                       ttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtt                                                    <  1:104787/149‑1 (MQ=255)
                                                                                          ccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTATCCAACCTGGTTTTg                                                 >  1:426871/1‑149 (MQ=255)
                                                                                           cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCATTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                <  2:51714/149‑1 (MQ=255)
                                                                                           cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                <  2:505881/149‑1 (MQ=255)
                                                                                            aGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTggg                                               >  2:867688/1‑149 (MQ=255)
                                                                                             gACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGGGTTCTACCCCACCTGGTTTTGGGt                                              >  1:856972/1‑149 (MQ=255)
                                                                                                gaTAGTGGCTGCAAGCTGGTCCGGAACACCGAGAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCTTGGTGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGa                                           <  1:249043/148‑1 (MQ=255)
                                                                                                 aTAGGGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGACCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAt                                          <  1:27640/149‑1 (MQ=255)
                                                                                                        tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCa                                   >  2:634801/1‑149 (MQ=255)
                                                                                                         ttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTGGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCaa                                  <  2:88325/149‑1 (MQ=255)
                                                                                                           cACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAt                                <  1:269010/149‑1 (MQ=255)
                                                                                                            aCGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAtt                               >  1:609220/1‑149 (MQ=255)
                                                                                                              gCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcg                             <  1:90257/149‑1 (MQ=255)
                                                                                                              gCTGGTTCGTAATACCGATAGCTGCAAGTGGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcg                             <  1:372566/149‑1 (MQ=255)
                                                                                                              cgtGGTTCGTAATACCGATAGCGGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcg                             <  2:153782/147‑1 (MQ=255)
                                                                                                                   ttCGTTATCCCGATAGCCGCAATTTGAGCCGAACCGATCTCCGCTTTCGCCAGCCCTTCTACCCGCGTTGAGCTGTTGGTGGCCTAGCTTTCCATTGGGGCGTTTGCTACCCAACCTTGTTTTGGGTAGATTTGCTCAAATTCGCGCTg                        <  2:73058/149‑1 (MQ=255)
                                                                                                                   ttCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTg                        >  2:225987/1‑149 (MQ=255)
                                                                                                                      gTAATACCGATAGCTGCGATTTGAACGGAACTTATAGCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                     <  2:892801/149‑1 (MQ=255)
                                                                                                                            ccGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCt               >  2:140143/1‑149 (MQ=255)
                                                                                                                                     gCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACGCGCTAATGATAtt      >  1:303146/1‑149 (MQ=255)
                                                                                                                                       aaTTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAGTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGCCCCGCTAATGATATTgg    >  2:207826/1‑149 (MQ=255)
                                                                                                                                         tttGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCa  <  1:576917/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
ACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCA  >  NC_000913/4116862‑4117147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑