Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,093,040 G→A 100% A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,093,0400GA98.8% 134.2 / ‑6.5 49A215T (GCT→ACT) hisChistidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (25/22);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGT  >  NC_000913/2092897‑2093185
                                                                                                                                               |                                                                                                                                                  
aTTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑tttt                                                                                                                                              <  1:285450/149‑1 (MQ=255)
 ttttCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTg                                                                                                                                             <  1:334252/149‑1 (MQ=255)
     cGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGctct                                                                                                                                         >  1:637999/1‑149 (MQ=255)
          ccTGCTGGAGTTAACCCGCGGTAAGGCGATTGTTGTTGCCGATGAAGCCTATATCGCGTTTTACCCGCATGCATCCTTGACTGTCTGGCTGGCGGAATATCCACACCTGACTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCgg                                                                                                                                    >  1:194267/1‑149 (MQ=255)
          ccTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCGATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCgg                                                                                                                                    >  2:489376/1‑149 (MQ=255)
                        cccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGGTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCGGCACCTGGCTATTTTACGCACACTGTCGAACAC‑TTTTGCTCTGGCGGGGCTTCCTTGCGGa                                                                                                                      >  1:529794/1‑149 (MQ=255)
                           gcgGTAAGGCGATTGTGGTTGCCGAGGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGGttt                                                                                                                   >  1:134845/1‑149 (MQ=255)
                              gTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCCCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACg                                                                                                                <  1:218616/149‑1 (MQ=255)
                              gTAAGGCGATTGTGGTCGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACg                                                                                                                <  2:291896/149‑1 (MQ=255)
                               tAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTACCCTTCGGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGc                                                                                                               <  1:891163/149‑1 (MQ=255)
                                  ggCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTAGGCTgg                                                                                                            >  1:211356/1‑149 (MQ=255)
                                  ggCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTgg                                                                                                            <  1:240990/149‑1 (MQ=255)
                                   gCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCGGGc                                                                                                           >  1:135160/1‑149 (MQ=255)
                                       ttGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTGGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTACGGTCTTAGGCGGGCagca                                                                                                       >  1:883679/1‑146 (MQ=255)
                                          tgGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaa                                                                                                    >  2:141808/1‑149 (MQ=255)
                                           ggTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaag                                                                                                   >  1:814550/1‑149 (MQ=255)
                                             ttGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                                 <  1:732281/149‑1 (MQ=255)
                                             ttGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                                 <  2:401815/149‑1 (MQ=255)
                                                ccGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGta                                                                                              <  1:865535/149‑2 (MQ=255)
                                                   aTGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGtcatc                                                                                           >  1:645879/1‑149 (MQ=255)
                                                   aTGAAGCCGTTATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATCTCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTGTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGtcatc                                                                                           <  2:235108/149‑1 (MQ=255)
                                                     gAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCaa                                                                                         <  2:817381/149‑1 (MQ=255)
                                                           tataTCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctgct                                                                                   >  1:87099/1‑149 (MQ=255)
                                                             tttCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtga                                                                                 <  2:353096/147‑1 (MQ=255)
                                                             tcgcGAGTCTTGCCCGCAGGCATCGCTGGCTGGCGGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtga                                                                                 <  1:318765/146‑1 (MQ=255)
                                                              atCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTGACGCGCACTGTCGAAAGC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAACCGAAGAAGTCATCAACctgagggg                                                                                >  2:7546/1‑144 (MQ=255)
                                                               tcggggtttGCCCCCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGTCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtgatg                                                                               <  1:639061/143‑1 (MQ=255)
                                                                  aGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCTAGGTTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaaa                                                                            <  1:855764/149‑1 (MQ=255)
                                                                          ccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGc                                                                    >  1:827536/1‑149 (MQ=255)
                                                                          ccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGc                                                                    >  2:704552/1‑149 (MQ=255)
                                                                           cGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGGCATCAACCTGCTGATGAAAGTGATCGcc                                                                   <  1:489376/149‑1 (MQ=255)
                                                                                  atagctgggtggctggctgGCGGAATATCCGCAACTGGCTATTTTACGAACAATGTCGAAAAC‑AATTGAACTGGCGGGGCTTAGTTGCGGATTTACGCTGGCAGACGAAGAGGTCATCAACCTGATGCTGAAAGTGATCGCCACCTTcc                                                            >  2:313445/4‑149 (MQ=255)
                                                                                    cGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCg                                                          >  1:298949/1‑149 (MQ=255)
                                                                                        ggcgggctggctggcGGAATATCCGCACCTGGCTATTTTCCGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGctct                                                      <  2:469178/149‑1 (MQ=255)
                                                                                               tggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTACGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCAACCCGCTCTCGACAcc                                               >  2:613917/1‑149 (MQ=255)
                                                                                                ggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCg                                              <  1:797905/149‑1 (MQ=255)
                                                                                                 gctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATGAACCTGCGGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCgg                                             >  1:479186/1‑149 (MQ=255)
                                                                                                 gctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCgg                                             >  2:52304/1‑149 (MQ=255)
                                                                                                   tggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGtt                                           >  1:609387/1‑149 (MQ=255)
                                                                                                   tggcGGAATAGCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGTGTATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGAGCGCCCCCTACCCGCTCTCGACGCCGGtt                                           <  1:430285/149‑1 (MQ=255)
                                                                                                          atatCCGCACCTGGCTATTTTACGCACACTGTC‑AACGCTTTTTGCTGTGGCAGGGCCTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCCACCCGCCCTCGACGCCGGTTGCCGACa                                    <  2:192252/149‑1 (MQ=255)
                                                                                                             tCCGCACCTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTg                                 >  1:717913/1‑149 (MQ=255)
                                                                                                                gCACCGGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCGTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGACGAGCTTGCgg                              >  2:468719/1‑149 (MQ=255)
                                                                                                                    cTGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTAATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCa                          >  1:440332/1‑149 (MQ=255)
                                                                                                                     tGGCTATTTTACGCACACTGTCGAAAAC‑TTTTGCGCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAg                         <  1:746945/149‑1 (MQ=255)
                                                                                                                         tATTTTACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGGTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGt                     >  2:661684/1‑149 (MQ=255)
                                                                                                                           ttttACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGCCGCCGGTTGCCGCCATTGCGGCCCAGGAGTTa                   >  2:545503/1‑149 (MQ=255)
                                                                                                                            tttACGCACACTGTCGAAAAC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTaa                  <  1:141808/149‑1 (MQ=255)
                                                                                                                                    cacTGTCGAAAAC‑TGTTGCTCTCGCTGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAg          <  2:162679/149‑1 (MQ=255)
                                                                                                                                            aaaaC‑TTTTGCACTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTACTGATGAAAGTGATCGCCCCCTACCCGCTCTCGTCGCCGGTTGCCGACCTTGCGGCCCAGGCGTTAGGCCCACAGGGAAtcgt  >  1:375309/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
ATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGC‑TTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGT  >  NC_000913/2092897‑2093185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑