Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 202,885 C→T 5.3% T109M (ACG→ATG)  lpxA → UDP‑N‑acetylglucosamine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913202,8850CT 5.3% 219.8 / 2.5 76T109M (ACG→ATG) lpxAUDP‑N‑acetylglucosamine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (33/38);  new base T (2/2);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

AATGAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCA  >  NC_000913/202737‑203033
                                                                                                                                                    |                                                                                                                                                    
aaTGAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGCTCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGAc                                                                                                                                                      <  2:338976/149‑1 (MQ=255)
   gagaTTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTAGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAATCGTCACCATTCCTCGTGGCACAGCCCAGGGCGGTGGTTTGACGaa                                                                                                                                                   >  1:245672/1‑149 (MQ=255)
    agaTTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAg                                                                                                                                                  <  1:673967/149‑1 (MQ=255)
     gaTTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGGGGATTGACGAAgg                                                                                                                                                 >  2:60818/1‑149 (MQ=255)
      aTTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGAGTGAGGAAggg                                                                                                                                                >  2:461602/1‑148 (MQ=255)
       tttATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGATGAAGGTg                                                                                                                                               >  2:81465/1‑149 (MQ=255)
       tttATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTg                                                                                                                                               >  2:501388/1‑149 (MQ=255)
       gttATCAGTTCCTCTCCCTCGGCGAATTGAGCCAGAAGCGGAAATATGCTGGCGAACCGACCCGTCTGGAAATCGGCGATCGTAACCCGATACGCGAAAGCGTCACCATTCATCGTGGCACAGACCAGGGCGGTGGATTGACGAAGGTg                                                                                                                                               <  2:661082/148‑1 (MQ=255)
            cAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAg                                                                                                                                          >  1:542576/1‑149 (MQ=255)
             aGTTCGCCTCCATCGGGGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGGGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCAAAGTCCAGGGTGGTGTATTGACGAAGGTGGGCAGc                                                                                                                                         <  2:8322/149‑1 (MQ=255)
               ttCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGa                                                                                                                                       >  2:44730/1‑149 (MQ=255)
                  gCCCCCATCGGCGAAGTCAACCAGGATCGGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACaa                                                                                                                                    <  2:229939/149‑1 (MQ=255)
                   ccTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGGGGATTGACGAAGGTGGGCAGCGACAAc                                                                                                                                   >  1:74555/1‑149 (MQ=255)
                            gctaatgtAACCAGCATCTTAAATCTGCTGGCGAACCGACCCGTGGTGAAATCGGCGAGCGTAACCGCATTCGCGAAAGCGTCCCCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACtgatg                                                                                                                          <  1:191645/142‑1 (MQ=255)
                            gCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACtgatg                                                                                                                          <  1:155680/149‑1 (MQ=255)
                              taggtcaaCCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACTATGGTGGGCAGCGACAACTTACTgatgat                                                                                                                        <  2:199629/143‑1 (MQ=255)
                              gAAGTTAACCAGGATCTGAAATATGCCGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTgatgat                                                                                                                        <  2:250179/149‑1 (MQ=255)
                              gAAGTTAACCACGATCCGAAATATGCTGGCGACCCGCCCCGTGTGGAACTCGGCGATCGTAACCGCAGTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTgatgat                                                                                                                        <  2:3611/149‑1 (MQ=255)
                                aGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCTACGACTTACTGATGATCa                                                                                                                      <  1:670155/149‑1 (MQ=255)
                                 gTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGAGAGCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACTAAGGTGGGCAGCGACAACTTACTGATGATCaa                                                                                                                     >  2:504992/1‑149 (MQ=255)
                                  ttAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAAc                                                                                                                    <  1:224251/149‑1 (MQ=255)
                                  ttAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAAc                                                                                                                    >  1:908039/1‑149 (MQ=255)
                                  ttAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGAGGATCAAc                                                                                                                    >  2:160421/1‑149 (MQ=255)
                                   tAACCAGGATCTGAAATATGCTGGCGAACCGACTCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATGCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAAcg                                                                                                                   <  2:430020/149‑1 (MQ=255)
                                   tAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAAcg                                                                                                                   <  1:803781/149‑1 (MQ=255)
                                       cAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGca                                                                                                               >  1:904393/1‑149 (MQ=255)
                                        aGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGCCGCGGGTGGGCGGCGGCGAGTTACTGCTGATCAAcggggac                                                                                                              >  1:476989/1‑144 (MQ=255)
                                        aGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGcac                                                                                                              >  2:501256/1‑149 (MQ=255)
                                          gATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGGGCACAt                                                                                                            >  1:86896/1‑149 (MQ=255)
                                             cTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGAGTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTgc                                                                                                         >  2:777556/1‑149 (MQ=255)
                                             cTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGGCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTgc                                                                                                         >  2:661834/1‑149 (MQ=255)
                                              tGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTCCTGATGATCAACGCGCACATTgcg                                                                                                        >  1:288162/1‑149 (MQ=255)
                                                       cTGGCGAACCGACCCGTGTGGAAACCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGCGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGt                                                                                               <  2:547462/149‑1 (MQ=255)
                                                          gCGAACCGACCGTGGTTGAAATCTGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAATGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCAGGATTGTACg                                                                                            <  2:398630/149‑1 (MQ=255)
                                                           ccaaCCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACgg                                                                                           <  1:523422/147‑1 (MQ=255)
                                                                   cccGTGTGGACATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAAc                                                                                   <  1:83905/149‑1 (MQ=255)
                                                                    ccGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAAcc                                                                                  >  2:422048/1‑149 (MQ=255)
                                                                     cGTGTGGAAAGCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCACACGATTGTACGGTAGGTAACCg                                                                                 <  1:656126/149‑1 (MQ=255)
                                                                       tgtgGAAATCGGCGATCGTTACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTACCCGcc                                                                               <  2:469176/149‑2 (MQ=255)
                                                                       tgtgGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCt                                                                               <  1:283695/149‑1 (MQ=255)
                                                                         tgGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGt                                                                             <  2:480519/149‑1 (MQ=255)
                                                                            aaaTCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGGCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTAtt                                                                          <  2:754511/149‑1 (MQ=255)
                                                                             aaTCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATtc                                                                         >  1:254371/1‑149 (MQ=255)
                                                                              aTCGGCGATCGTAACCGCCTTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATtct                                                                        <  2:240249/149‑1 (MQ=255)
                                                                               tCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGATGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATtctc                                                                       >  1:552798/1‑149 (MQ=255)
                                                                               tCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATtctc                                                                       <  1:385674/149‑1 (MQ=255)
                                                                               tCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATtctc                                                                       >  1:360857/1‑149 (MQ=255)
                                                                                 ggCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGc                                                                     <  1:501388/149‑1 (MQ=255)
                                                                                 ggCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGACAGCGACAACTTACTGATGATGAACGCGCACGTTGCGCACGATTGTACGGTAGAAAAACGCGGTATTCTCGc                                                                     >  2:249451/1‑149 (MQ=255)
                                                                                  gCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTTACCGCGGTGTTCTTGcc                                                                    >  1:66357/1‑149 (MQ=255)
                                                                                  gCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACCCGCACATTGCGCACGCTTGTACGGTAGGTAACCGCTCGATTCTCGcc                                                                    >  2:301830/1‑149 (MQ=255)
                                                                                     aTCGTACCCGCATTCGCGGACGCGTCACCATGGAGTGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCATCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCcaac                                                                 <  2:458662/149‑1 (MQ=255)
                                                                                            ccGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGATGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAAcaacgcaa                                                          <  1:81465/149‑1 (MQ=255)
                                                                                                  tCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACCTTGCGCACGATTGTACGGTAGGTACCCGCGGTATTCTTGCCAACAACGCATCGCTgg                                                    <  2:312083/149‑1 (MQ=255)
                                                                                                    gcgAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGAGTGTACGGTAGGTAACGGCTGTATTCTCGACAACAACGCAAAGCTGGCg                                                  >  1:121293/1‑149 (MQ=255)
                                                                                                     cgAAAGCGTCACCATTCATCGTGGCACAGGCCAGGTCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCgg                                                 >  2:511282/1‑149 (MQ=255)
                                                                                                                   ttCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGtt                                   <  1:692386/149‑1 (MQ=255)
                                                                                                                    tCATCGTGGCACAGTCCAGGGCGGTGGATTGATGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTg                                  <  2:552798/149‑1 (MQ=255)
                                                                                                                    tCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGAGTGTACGGTAGGTAACCCCGGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTg                                  >  2:183249/1‑149 (MQ=255)
                                                                                                                             cacaGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTcg                         <  1:151728/149‑1 (MQ=255)
                                                                                                                              acaGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTcgc                        <  2:748554/149‑1 (MQ=255)
                                                                                                                               caGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTcgcg                       >  2:838090/1‑149 (MQ=255)
                                                                                                                               caGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACCTcgcg                       >  2:779997/1‑149 (MQ=255)
                                                                                                                               caGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGCCACGTATCGGTTGACGACTTcgcg                       <  1:461602/149‑1 (MQ=255)
                                                                                                                               caGTCCAGGGCCGTGGATTGACGAAGGTGGGCAGCGACAACTTATTGCTGATCAACGCGCACCTAAAGCCCTATTGTACGGTATTTAACCTCTTTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTcgcg                       <  1:668093/149‑1 (MQ=255)
                                                                                                                                aGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGa                      >  1:763789/1‑149 (MQ=255)
                                                                                                                                 gTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCCACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGat                     <  2:896369/149‑1 (MQ=255)
                                                                                                                                     aGGGCGGTGGATTGACGAAGGTGGGCAGCAACAACTTACTGATGATCAACGCGCACATTGCGCACGACTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGAATCGGTTGACGACTTCGCGatcatc                 >  1:560041/1‑149 (MQ=255)
                                                                                                                                      gggCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATcg                >  1:885446/1‑149 (MQ=255)
                                                                                                                                         cGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAATAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATcggcg             <  1:112909/149‑1 (MQ=255)
                                                                                                                                          ggTGGGTTGACGAAGGTGGGCAGCGACACCTTCCTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATcggcgg            <  1:709550/149‑1 (MQ=255)
                                                                                                                                          ggTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATcggcgg            <  2:208712/149‑1 (MQ=255)
                                                                                                                                           gTGGCTTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCggcggc           <  2:901098/149‑1 (MQ=255)
                                                                                                                                              gATGGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGAGTGGACGGTAGGTGACCGCTGTATTCTAGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGCCGACTTCGCGCTCCTCggcggccga        >  2:190268/1‑146 (MQ=255)
                                                                                                                                               aTTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTCACGACTTCGCGGCCATCGGCGGCacgc       >  2:33080/1‑146 (MQ=255)
                                                                                                                                                 tGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGAcc     <  2:697873/149‑1 (MQ=255)
                                                                                                                                                 tGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGAATCGGTTGACGACTTCGGGATCATCGGCGGCATGAcc     >  2:437953/1‑149 (MQ=255)
                                                                                                                                                    cGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCa  <  2:74555/149‑1 (MQ=255)
                                                                                                                                                    cGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCACGCCCGCa  <  2:489556/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AATGAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCA  >  NC_000913/202737‑203033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑