Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,413,542 A→T Y67N (TAT→AAT)  ydaC ← DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,413,5420AT98.0% 339.2 / NA 102Y67N (TAT→AAT) ydaCDUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (58/42);  minor base G (1/1);  total (59/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

GCGAAGCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTC  >  NC_000913/1413396‑1413687
                                                                                                                                                  |                                                                                                                                                 
gCGAAGCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCa                                                                                                                                                 <  1:11397/149‑1 (MQ=255)
 ccaaGCATCCGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTCAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTACTTTGAATTCaa                                                                                                                                                <  2:1341429/147‑1 (MQ=255)
 cGAAGCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCaa                                                                                                                                                <  2:1188143/149‑1 (MQ=255)
 cGAAGCATCAGGCCGTATTCAACCATCCAATCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGAAGCCCGATGTTGATTATTAATCATCAGCTCTGAAGTATTAATTTGAATTCaa                                                                                                                                                >  1:1173654/1‑149 (MQ=255)
   aaGCATCAGGCCGTATTCAACCATCCACTCTGCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATg                                                                                                                                              <  1:1196716/149‑1 (MQ=255)
      cATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGCTAGACGGAAAATGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCaa                                                                                                                                           <  1:422999/149‑1 (MQ=255)
             ccGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCGGGCGCCAATGTTAGACTGAAATTGACTCCAGATAATTTTTATTAATAATCAGCTATGAAGTTTTAATTTTAATTCAATGCAATtcgcta                                                                                                                                    >  1:341120/1‑145 (MQ=255)
                   tCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTg                                                                                                                              >  1:1448446/1‑149 (MQ=255)
                     agccATCCACTCTTCATTAAGGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGACATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGCATTCAATACAATTCTCGAGGGCTGaa                                                                                                                            >  2:506973/3‑149 (MQ=255)
                     aaCCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCCATGCAATTTTCGAAGACTGaa                                                                                                                            >  1:65350/1‑149 (MQ=255)
                     aaCCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGaa                                                                                                                            >  1:623792/1‑149 (MQ=255)
                     aaCCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGaa                                                                                                                            <  1:1557117/149‑1 (MQ=255)
                     aaCCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGaa                                                                                                                            >  1:741975/1‑149 (MQ=255)
                      aCCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAg                                                                                                                           >  1:1303136/1‑149 (MQ=255)
                        cATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGtt                                                                                                                         >  1:545319/1‑149 (MQ=255)
                         aTCCCCTCTTCATCAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGttt                                                                                                                        <  1:165984/149‑1 (MQ=255)
                            cacaCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATGAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTtctc                                                                                                                     <  2:208287/149‑1 (MQ=255)
                             gccctTCATGAAAGATTACTTGTGCCATCGCATCACCGCTGGCCGGCGCCAATGGTAGACTGAAATTGACGCCCGACGCTGATGATCAATAATCAGCTATGAACTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGGTTCTCg                                                                                                                    <  2:4846/146‑1 (MQ=255)
                                 ttCATTAAATCTTACTTGGGCCATCGCATCACCTCTGGCAGGCGCCAATTTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGCAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAAt                                                                                                                <  2:1749982/149‑1 (MQ=255)
                                 ttCATTAAAGATTACTTGTGCCATCGCATCACCTCGGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAAt                                                                                                                >  2:1115141/1‑149 (MQ=255)
                                    aTTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTaa                                                                                                             >  1:198542/1‑149 (MQ=255)
                                    aTTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACACCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTACTTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTGTCGCAATTaa                                                                                                             >  1:863714/1‑149 (MQ=255)
                                     ttAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTaaa                                                                                                            >  1:727978/1‑149 (MQ=255)
                                      tAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTaaac                                                                                                           >  2:1068991/1‑148 (MQ=255)
                                            ttACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATc                                                                                                     >  2:1119154/1‑149 (MQ=255)
                                             tACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCa                                                                                                    <  1:1434488/149‑1 (MQ=255)
                                             gaCTTGGGCCACCGCNTCACCTCCTGCAGGCTCCAATGTTAGACTGAGATTGACGCCCGATGTTGATTATTAAGAACCAGCTATGAAGCGTTAAATCGACTGCGATACAGTTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCa                                                                                                    <  1:1755531/148‑1 (MQ=255)
                                               cTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGt                                                                                                  >  1:979592/1‑149 (MQ=255)
                                                  gtgCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGtttt                                                                                               >  2:189482/1‑149 (MQ=255)
                                                  gtgCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGtttt                                                                                               >  1:840971/1‑149 (MQ=255)
                                                  gtgCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTACCAGtttt                                                                                               >  1:710296/1‑149 (MQ=255)
                                                  gtgCCATAGCAGTACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGCCTGAAGTTGCTCGCAATTAAAATTTATCAGtttt                                                                                               >  2:1185368/1‑149 (MQ=255)
                                                    gCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTAc                                                                                             <  1:972839/149‑1 (MQ=255)
                                                    gCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTAc                                                                                             >  1:1503037/1‑149 (MQ=255)
                                                    gCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTAc                                                                                             <  1:1370717/149‑1 (MQ=255)
                                                    gCCATCGCAGCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTCATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTAc                                                                                             <  2:1105110/149‑1 (MQ=255)
                                                     ccATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACt                                                                                            >  2:1059045/1‑149 (MQ=255)
                                                     ccATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACt                                                                                            >  1:1356567/1‑149 (MQ=255)
                                                      cATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCACTTAAAATTTATCAGTTTTACtt                                                                                           >  2:371702/1‑149 (MQ=255)
                                                       aTCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACttt                                                                                          <  1:1744060/149‑1 (MQ=255)
                                                       aTCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACttt                                                                                          >  1:1449802/1‑149 (MQ=255)
                                                       aTCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATATTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACttt                                                                                          >  1:427081/1‑149 (MQ=255)
                                                        tCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTc                                                                                         >  1:260225/1‑149 (MQ=255)
                                                         cGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCt                                                                                        <  1:1343154/149‑1 (MQ=255)
                                                          gCATCGTCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAGTTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTg                                                                                       <  2:1694357/149‑1 (MQ=255)
                                                            aTCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGct                                                                                     >  2:1409494/1‑149 (MQ=255)
                                                                ccTCTGGCAGGCTCCAATGTTAGACTGAAATTGACGCCCGGTGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGCATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGctctct                                                                                 <  2:514038/149‑1 (MQ=255)
                                                                  tctGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGTTTATTAATAACCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAGAATTTATCAGTTTAACTTTCTGCTCTCTgg                                                                               >  1:1179213/1‑149 (MQ=255)
                                                                  tctGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTgg                                                                               >  1:1179841/1‑149 (MQ=255)
                                                                  tctGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTgg                                                                               >  2:917443/1‑149 (MQ=255)
                                                                    tGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGaa                                                                             >  1:1107935/1‑149 (MQ=255)
                                                                               aaTGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAACCGCCTGcttc                                                                  >  2:647980/1‑149 (MQ=255)
                                                                                  gTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCttt                                                               <  1:1769149/149‑1 (MQ=255)
                                                                                   ttAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCtttt                                                              >  2:285734/1‑149 (MQ=255)
                                                                                   ttAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCtttt                                                              <  1:284156/149‑1 (MQ=255)
                                                                                         tGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCt                                                        >  2:1741990/1‑149 (MQ=255)
                                                                                         tGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCt                                                        >  1:191429/1‑149 (MQ=255)
                                                                                           agaTTGACGCCCGATTTTGATTATTAATAATCAGCTATTAAGTTTTAATTTGAATTCAATGCAATTCTCGGGGACTGAAGTTTCTCGCGATTAAAATTTATCAGTTTGTCTTGCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTga                                                      <  2:426292/147‑1 (MQ=255)
                                                                                           aacttGACGCCCGATGTTGATTATTAATAATCATCTATGAAGTTTTAATGTGAATTCAATGCTATTCGCTAGGACTTAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTga                                                      <  2:1230149/146‑1 (MQ=255)
                                                                                             actGACGCACGATGTTGATTATTAATAATCAGCTATGAAGCTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTgaga                                                    <  1:301466/147‑1 (MQ=255)
                                                                                             aTTGACTCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTgaga                                                    <  2:664643/149‑1 (MQ=255)
                                                                                               tGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGc                                                  <  1:61574/149‑1 (MQ=255)
                                                                                                     ccGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGAGAGCAttttt                                            >  2:207449/1‑149 (MQ=255)
                                                                                                     ccGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCAttttt                                            <  1:1175644/149‑1 (MQ=255)
                                                                                                       gctGTTGATTATTAATAATCAGCTATGAATTTTTACTTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTc                                          <  2:341120/147‑1 (MQ=255)
                                                                                                             gATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTc                                    >  2:1205788/1‑149 (MQ=255)
                                                                                                             gATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTCCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTc                                    >  1:877264/1‑149 (MQ=255)
                                                                                                             gATTATTAATAATCAGCGATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGCAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTc                                    >  1:1591781/1‑149 (MQ=255)
                                                                                                              attattAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCACTTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCt                                   >  1:663578/1‑149 (MQ=255)
                                                                                                                tattaATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTATCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGa                                 >  1:1222207/1‑149 (MQ=255)
                                                                                                                   taataaTCAGCTATGAAGTTTTAATATGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGAttt                              >  1:1063133/1‑149 (MQ=255)
                                                                                                                    aataatCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTAAGAGCATTTTTTCGCATTCTGCTTTc                             >  1:223826/1‑149 (MQ=255)
                                                                                                                     ataatCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCg                            <  1:917443/149‑1 (MQ=255)
                                                                                                                     ataatCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCg                            <  2:623792/149‑1 (MQ=255)
                                                                                                                      taatCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGt                           >  1:1516761/1‑149 (MQ=255)
                                                                                                                           aGCTATGAAGTGTTAATTTGAATTCAATGCAATTCTCGAGGACTTAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGtt                      <  2:265392/149‑1 (MQ=255)
                                                                                                                            gCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGttt                     <  2:1068959/149‑1 (MQ=255)
                                                                                                                              tATGAAGTTTTAATTTGAATTCAATGCAATTCTCGCGGGCTGGAGTGTCTCGTAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAg                   <  2:1701900/149‑1 (MQ=255)
                                                                                                                                tGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGAt                 >  2:905944/1‑149 (MQ=255)
                                                                                                                                 gAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGAtt                >  1:1388041/1‑149 (MQ=255)
                                                                                                                                 gAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGAtt                <  2:1013450/149‑1 (MQ=255)
                                                                                                                                 gAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGAtt                >  1:1381404/1‑149 (MQ=255)
                                                                                                                                    gTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAATCGCCTGCTCCATTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTg             <  1:1229698/149‑1 (MQ=255)
                                                                                                                                    gTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGTTTTTTGTTTTCGTTAGTTTAGGTTTTg             >  2:1249989/1‑149 (MQ=255)
                                                                                                                                     ttttAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGCTTTCGTTAGTTTAGATTTTGa            >  2:505277/1‑149 (MQ=255)
                                                                                                                                     ttttAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGGTTTTgc            >  2:329678/1‑148 (MQ=255)
                                                                                                                                     ttttAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGa            <  2:1124388/149‑1 (MQ=255)
                                                                                                                                     ttttAATTTGAATTCAATGCAATTCTCGACGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGa            <  1:395435/149‑1 (MQ=255)
                                                                                                                                       ttAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTATCTTTTTTCTTTTTTTAGCATTCTGCTTTCGTTAGTTTAGATTTTgtct          >  2:311307/1‑146 (MQ=255)
                                                                                                                                       ttAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAat          >  2:304696/1‑149 (MQ=255)
                                                                                                                                          aTTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAGTTTATCAGTTTTACTTTCTGCTCGCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCGAATTTCGTTAGTTTAGATTTTGAATTTc       >  2:430341/1‑149 (MQ=255)
                                                                                                                                          aTTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATc       >  2:454746/1‑149 (MQ=255)
                                                                                                                                          aTTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATc       <  1:508151/149‑1 (MQ=255)
                                                                                                                                          aTTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAAAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGGTTTTGAATATc       >  2:1629779/1‑149 (MQ=255)
                                                                                                                                            ttGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCtt     <  2:573334/149‑1 (MQ=255)
                                                                                                                                            ttGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCtt     <  2:1448446/149‑1 (MQ=255)
                                                                                                                                            ttGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCtt     >  1:968064/1‑149 (MQ=255)
                                                                                                                                             tGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTg    <  2:684871/149‑1 (MQ=255)
                                                                                                                                               aaTTCAATGCCATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTGATCTGTGAGCATTTTTTCGCATTCCGATTTCGTTAGTTTAGATTTTGAATATCTTGta  <  2:65350/149‑2 (MQ=255)
                                                                                                                                               aaTTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTc  >  1:545476/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCGAAGCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTC  >  NC_000913/1413396‑1413687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: