Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 317,820 A→G 9.9% L167P (CTG→CCG)  rclC ← reactive chlorine species (RCS) stress resistance inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913317,8200AG 9.9% 103.1 / 2.9 42L167P (CTG→CCG) rclCreactive chlorine species (RCS) stress resistance inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (18/19);  new base G (2/2);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

AAAGGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCATGCCTCCGGGGTGGTGATTAAAAATGAGAGTGTTACCAGCGGCGTGGTAAATGCCATC  >  NC_000913/317676‑317963
                                                                                                                                                |                                                                                                                                               
aaaGGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCAGAATCTGCCATTATCATGACTGCACCTGCCAGCAt                                                                                                                                             <  2:497785/149‑1 (MQ=255)
   gggggCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTCCGGAATCACGAAAAGCAGCAGTCAACGGTGCACTACCTTCATCACTACGACGGTTAAGAGTACCGCGCGCCGAATCTGCGCTTATCATGACTGCACCTGCCAGCATCag                                                                                                                                          >  2:586949/1‑149 (MQ=255)
    ggggCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCaga                                                                                                                                         <  1:706632/149‑1 (MQ=255)
    ggggCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCaga                                                                                                                                         <  1:706694/149‑1 (MQ=255)
    ggggCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCaga                                                                                                                                         <  1:791793/149‑1 (MQ=255)
          ttaCGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCt                                                                                                                                   <  1:238048/147‑1 (MQ=255)
          tGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCt                                                                                                                                   <  1:271581/149‑1 (MQ=255)
            aCGCCTGAAAAAGTGCACAACAGACAGTGTTCGTATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCttt                                                                                                                                 <  1:745963/149‑1 (MQ=255)
               ccTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCaa                                                                                                                              >  1:777618/1‑149 (MQ=255)
                 tGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATa                                                                                                                            >  1:246451/1‑149 (MQ=255)
                             caacaGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAg                                                                                                                >  1:106876/1‑149 (MQ=255)
                             caacaGACAGTGTTCGGATTATCAATATTAAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCAGGACTGCACATGCCTCAATCAGAGTATCTTTCATTACCAGGCGACCAg                                                                                                                >  1:502695/1‑149 (MQ=255)
                                cagacagTGTTCGGATTATCAATATTCAGTTTTTAATGTTGAACTGGATTCATTACTGCGTTGATTAAGAATTTCCCGCGCCGAATCTGCCATTCTCATGACTGCACCTGCCAGCATCAGCGGAGCTTTCATTACCAGGCGCCCAGCAc                                                                                                             >  2:459443/1‑149 (MQ=255)
                                   acagTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCGGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCgg                                                                                                          >  2:235750/1‑149 (MQ=255)
                                   acagTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCGGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCgg                                                                                                          >  2:235666/1‑149 (MQ=255)
                                      gggtTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGata                                                                                                       <  1:47227/147‑1 (MQ=255)
                                        gtTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGataaa                                                                                                     >  1:688780/1‑149 (MQ=255)
                                             gATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTGGTTTAAGAATTTCCCGGGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAgg                                                                                                >  2:730295/1‑149 (MQ=255)
                                                        ttCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCGGCCATTATCATGACTGCACCTGCCAGCATCAGAGCATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCatgat                                                                                     <  1:35824/149‑1 (MQ=255)
                                                            gTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGc                                                                                 <  2:717918/149‑1 (MQ=255)
                                                            gTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCAATTATCATGACTGCACCTGCCAGCATCAGAGTATATTTCCATACCACGCGACCAGCACCGGCTAAATAAGGGAAACCATGATGAGc                                                                                 >  1:560224/1‑149 (MQ=255)
                                                              ttttAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTAGCATGACTGCACCTGCCAGCATCAGAGTAACTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGCTGAGCGt                                                                               >  2:771108/1‑149 (MQ=255)
                                                               tttAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCCATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTc                                                                              >  1:747495/1‑149 (MQ=255)
                                                               tttAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTc                                                                              <  1:661910/149‑1 (MQ=255)
                                                               tttAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTc                                                                              >  1:196094/1‑149 (MQ=255)
                                                                                  cATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCATCACCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCAt                                                           >  1:449395/1‑149 (MQ=255)
                                                                                        tGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCATGCCTcc                                                     >  1:17085/1‑149 (MQ=255)
                                                                                        tGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCATGCCTcc                                                     >  1:368271/1‑149 (MQ=255)
                                                                                                      aTTCCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCATGCCTCCGGGGTGGTGATTaa                                       <  2:230324/149‑1 (MQ=255)
                                                                                                       tttCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCATGCCTCCGGGGGGGTGATTaaa                                      >  1:365874/1‑149 (MQ=255)
                                                                                                            cgcgCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCATGCCTCCGGGGTGGTGATTAAAAATga                                 <  1:615579/149‑1 (MQ=255)
                                                                                                                     ccTGCCATTATCATGACTGCCCCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCATGCCTCCGGGGTGGTGATTAAAAATGAGAGTGTTAc                        <  2:747495/148‑1 (MQ=255)
                                                                                                                            ttATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGACAACGAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCATGCCTCCGGGGTGGTGATTAAAAATGAGAGTGTTACCAgcggc                 <  1:584127/149‑1 (MQ=255)
                                                                                                                               tCATGACTGCACCTGCCGGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCATGCCTCCGGGGTGGTGATTAAAAATGAGAGTATTACCAGCGGCGTg              <  1:235750/149‑1 (MQ=255)
                                                                                                                               tCATGACTGCACCTGCCGGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCATGCCTCCGGGGTGGTGATTAAAAATGAGAGTATTACCAGCGGCGTg              <  1:235666/149‑1 (MQ=255)
                                                                                                                               tCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCCCCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCATCCCTCCGGGGTGGTGATTAAAAATGAGAGTGTTACCAgcggcggg              >  1:558968/1‑147 (MQ=255)
                                                                                                                                       gCACCTGCCAGCATCAGAGTATTTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCATGCCTCCGGGGTGGTGATTAAAAATGAGAGTGTTACCAGCGGCGTGGTAAATGc      <  2:103486/149‑1 (MQ=255)
                                                                                                                                       gCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCATGCCTCCGGGGTGGTGATTAAAAATGAGAGTGTTACCAGCGGCGTGGTAAATGc      <  2:106876/149‑1 (MQ=255)
                                                                                                                                       gCACCTGCCAGCATCAGAGTATCGTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACGCATGCCTTCGGGGTGGTGATTAAAAATGAGAGTGTTACCAGCGGCGTGGTAAATGc      <  2:502695/149‑1 (MQ=255)
                                                                                                                                          ccTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGCCACCCAATGCGGGTACCCATGCCTCCGGGGTGGTGATTAAAAATGAGAGTGTTACCAGCGGCGTGGTAAATGCCAt   <  1:459443/149‑1 (MQ=255)
                                                                                                                                           cTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCATGCCTCCGGGGTGGTGATTAAAAATGAGAGTGTTACCAGCGGCGTGGTAAATGCCATc  >  2:144373/1‑149 (MQ=255)
                                                                                                                                           cTGCCAGCATCAGAGTATCTGTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTAACCCAATGCGGGTACCCATGCCTCCGGGGCGGCGATTAAAAATGAGAGTGTTACAAGCGGGGTGGTAAAAGCCaac  >  2:390217/1‑147 (MQ=255)
                                                                                                                                                |                                                                                                                                               
AAAGGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCACCTGCCAGCATCAGAGTATCTTTCAATACCAGGCGACCAGCACCGGATAAATAAGGGAAACCATGATGAGCGTCACCCAATGCGGGTACCCATGCCTCCGGGGTGGTGATTAAAAATGAGAGTGTTACCAGCGGCGTGGTAAATGCCATC  >  NC_000913/317676‑317963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑