Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,440,057 A→G 11.6% M387T (ATG→ACG)  ytfL ← UPF0053 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,440,0570AG11.6% 91.8 / 2.8 41M387T (ATG→ACG) ytfLUPF0053 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (18/17);  new base G (3/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

TTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACGGGCGACAATCTGCTCTTCCAGCCCCTGAC  >  NC_000913/4439914‑4440204
                                                                                                                                               |                                                                                                                                                   
ttGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTCTCGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCCCCGAGTCGGTGCGTTTCGGGATCTTACCCCGCATAAACCTCATa                                                                                                                                                >  1:471724/1‑149 (MQ=255)
ttGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATa                                                                                                                                                >  1:331927/1‑149 (MQ=255)
 tGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGTGGTAGTTATCGATATCAACCACTTCAAATTTGTAGCAGGCGAATTTCACCGAATCGGTGCGTTTCGGGCTCTTACGCAGCATAACcaacgtga                                                                                                                                               >  2:196457/1‑143 (MQ=255)
  ggCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATATCCACCACTTCAAAGTTGTAGCCGGCGAATTTCACCGAATCGCTGCGTTTCGGGATCTTACGCAGCATAAACATCATaaa                                                                                                                                              <  1:507737/149‑1 (MQ=255)
   gCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGAGCTTACGCAGCGTAACCGTCATaaat                                                                                                                                             >  1:261145/1‑148 (MQ=255)
   gCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATAGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAg                                                                                                                                             >  1:533401/1‑149 (MQ=255)
    cGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATATCCACCACTTCAACTTTGTAGCCGGCGAATTTCACCGAGTCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCTTaaatc                                                                                                                                            >  2:158071/1‑147 (MQ=255)
                         gTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGc                                                                                                                       >  2:465111/1‑149 (MQ=255)
                            gATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCg                                                                                                                    >  1:553096/1‑149 (MQ=255)
                             aTCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGa                                                                                                                   >  1:634749/1‑149 (MQ=255)
                               ccGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATATCCACCACTTCGAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACt                                                                                                                 <  1:546954/149‑1 (MQ=255)
                                      aCCAGCAGCTGGTCGATGCGGTAGGTATCGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGaa                                                                                                          <  1:265094/149‑1 (MQ=255)
                                       ccAGCAGCTGGTCGATGCGGTAGTTATCGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACGTCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAAc                                                                                                         >  2:474889/1‑149 (MQ=255)
                                       ccAGCAGCTGGTCGATGCGGTAGTTATCGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGAGCTTACGCAGCATAAACGTCATAAAGCCGCCGATGGTTTCGTAGTTGACCGACTGCGGGAAc                                                                                                         >  2:748839/1‑149 (MQ=255)
                                          gcagcTGGTCGATGCGGTAGTTATCGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCTCGACTGCGGGAACTCg                                                                                                      >  1:215056/1‑149 (MQ=255)
                                           cagcTGGTCGATGCGGTAGTTATCGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGt                                                                                                     >  1:448168/1‑149 (MQ=255)
                                                               tATCGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATg                                                                                 <  1:608082/149‑1 (MQ=255)
                                                                aTCGATATCCACCACTTAAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGTGATCTTACGCAGCATAAACATCATAAAGCCGCCGAAGGTTTCGTAGTTGCCCGACTGCGCGAACTCGTCAATATCCAGCACGCGCCTGa                                                                                >  2:590860/1‑149 (MQ=255)
                                                                aTCGATATCCACCACCACAAATTTGTAGCCGCCGAATTTCCCCGAACCACTGCATTTTGGCAACGTACGTAGCCTAAACATCATAAAGCCGCCGATGGTTTCCTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACCCGCATgc                                                                                >  2:171231/1‑148 (MQ=255)
                                                                 tCGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCGGCACGCGCATGAc                                                                               >  1:627518/1‑149 (MQ=255)
                                                                    atatCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGAcgtc                                                                            >  2:216154/1‑149 (MQ=255)
                                                                    atatCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGAcgtc                                                                            <  2:121891/149‑1 (MQ=255)
                                                                       tCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACgtcgtc                                                                         >  1:690564/1‑149 (MQ=255)
                                                                             acTTTAAATGCGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTgg                                                                   <  1:145620/149‑1 (MQ=255)
                                                                             acTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTgg                                                                   <  1:725759/149‑1 (MQ=255)
                                                                                tCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGAAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGt                                                                >  1:753909/1‑149 (MQ=255)
                                                                                    agttGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTgccg                                                            <  2:609698/147‑1 (MQ=255)
                                                                                            ccGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAAt                                                    <  2:30238/149‑1 (MQ=255)
                                                                                              ggCAAATTTCACCGAATCGGTGCTTTTCTGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCa                                                  <  2:553096/149‑1 (MQ=255)
                                                                                                  aaTTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCa                                              <  1:764676/149‑1 (MQ=255)
                                                                                                   aTTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCAt                                             <  2:679246/149‑1 (MQ=255)
                                                                                                    tttCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCACTTGGGGTGCGGCGGCCACTCAggcgtg                                            >  2:312188/1‑144 (MQ=255)
                                                                                                           gAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTtctc                                     >  1:751059/1‑149 (MQ=255)
                                                                                                                  tGCGTTTCGGGATGTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGACGCCGTCAATCAGCCATGAGTTCTCATCACgg                              >  1:545836/1‑149 (MQ=255)
                                                                                                                   gCGTTCCGGGCTCTTACGCAGCATAAACATCATACAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACggg                             <  1:559574/149‑1 (MQ=255)
                                                                                                                    cGTTTCGGGATCTTACGCAGCATAAACGTCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACGGGc                            <  1:474889/149‑1 (MQ=255)
                                                                                                                    cGTTTCGGGATCTTACGCAGCATAAACGTCATAAAGCCCCCGATGGTTTCGTAGTTGCCCGCCTGCGGGAACTCGCCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACGGGc                            <  1:748839/149‑1 (MQ=255)
                                                                                                                           ggATCTTACGCAGCATAAACATCATAAAGGCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACGGGCGACAATc                     >  1:507263/1‑149 (MQ=255)
                                                                                                                              tCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACGGGCGACAATCTGc                  <  2:533401/149‑1 (MQ=255)
                                                                                                                                   cGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGGCGTCCAAGAGCTATGAGTTCTCATCACGGGCGACAATCTGCTCTTc             <  1:792738/149‑1 (MQ=255)
                                                                                                                                        caTAAACATCATAAATCCTCCGATGGTTTCGCAGTTGCCCGACCGCGGGACCTCGTCACTATCCAGCACGCGCATGTCGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCTTGAGTTCTCATCACGGGCGACAATCTGCTCTTCCAGcc        <  1:44353/149‑1 (MQ=255)
                                                                                                                                         aTAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACGGGCGACAATCTGCTCTTCCAGccc       <  2:331927/149‑1 (MQ=255)
                                                                                                                                              catcacAAAGCCGCCGATGGTTTCGGAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACGGGCGACAATCTGCTCTTCCAGCCCCTgac  <  2:273325/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATATCCACCACTTCAAATTTGTAGCCGGCGAATTTCACCGAATCGGTGCGTTTCGGGATCTTACGCAGCATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACGGGCGACAATCTGCTCTTCCAGCCCCTGAC  >  NC_000913/4439914‑4440204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑