Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,227,241 T→A 5.8% Y1139N (TAT→AAT)  metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,227,2410TA 5.8% 191.4 / 2.3 68Y1139N (TAT→AAT) metHhomocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
Reads supporting (aligned to +/- strand):  ref base T (29/33);  new base A (2/2);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.40e-01

CTTGCCGACCGTTTAGCCGAAGCCTTTGCGGAGTATCTCCATGAGCGTGTGCGTAAAGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGC  >  NC_000913/4227097‑4227387
                                                                                                                                                |                                                                                                                                                  
cTTGCCGACCGTTTAGCCGAAGCCTTTGCGGAGTATCTCCATGAGCGTGTGCGTAAAGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCGATcc                                                                                                                                                >  1:608163/1‑149 (MQ=255)
     cGACCGTTTAGCCGAAGCCTTTGCGGAGTATCTCCATGAGCGTGTGCGTAAAGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCt                                                                                                                                           <  2:458804/149‑1 (MQ=255)
        ccGTTTAGCCGAAGCCTTTGCGGAGTATCTCCATGAGCGTGTGCGTAAAGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGcc                                                                                                                                        >  1:773665/1‑149 (MQ=255)
             tcgCCGAAGCCTTTGCGGAGTATCTCCATGAGCGTGTGCGTAAAGTCTACTGGGGCTATGCGCCGAACGAGAACCGCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGaa                                                                                                                                   <  2:679927/147‑1 (MQ=255)
                ccGAAGCCTTTGCGGAGTATCTCCATGAGCGTGTGCGTAAAGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCCCCGGGCTATCCGGCCTGCCCGGAACAt                                                                                                                                >  1:745660/1‑149 (MQ=255)
                   attccTCTGCGGAGTCTCTCTATGAGCGTGTGCGTACAGTCTACTGGGGCTATGCGCCGAACGAGACCCTCAGCAACGAAGCGCTGATCCGCGAAAACTACCCGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACAGACg                                                                                                                             <  2:786395/146‑1 (MQ=255)
                        tttGCGGAGAATCTCCATGAGCGTGTGCGTAAAGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGaaaa                                                                                                                        <  1:699770/149‑1 (MQ=255)
                                   tccccATAAGCGTGTGCGTAAAGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCt                                                                                                             <  1:325813/146‑1 (MQ=255)
                                    ctcCATGAGCGTGTGCGTAAAGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTg                                                                                                            >  1:681867/1‑149 (MQ=255)
                                     tcCATGAGCGTGTGCGTAACGTCTACTGGGGCTATGCGCCGAACGATAACCTCAGTAACGAAGAGCTGATCCGCGAAAACTACCAGGGAATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTgg                                                                                                           <  1:258091/149‑1 (MQ=255)
                                       cATGAGCGTGTGCGTAAAGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGa                                                                                                         <  1:626936/149‑1 (MQ=255)
                                           aGCGTGTGCGTAAAGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCAATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAgctg                                                                                                     >  1:400540/1‑149 (MQ=255)
                                           aGCGTGTGCGTAAAGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCAATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAgctg                                                                                                     >  1:569312/1‑149 (MQ=255)
                                             cGTGTGCGTAAAGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAACGCCACCACCTGGGAgctgct                                                                                                   >  2:733579/1‑149 (MQ=255)
                                                  gCGTAAAGTCTACAGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATAAGGAAAAAGCCACCATCTGGGAGCTGCTGGagg                                                                                              >  1:544766/1‑147 (MQ=255)
                                                   cGTAATGGCGACTGGGGCTGTGCGCCGAACGAGAACCTCAGCAACGAAGTGCCGATCCGCGAAAACTACCCGGGCTTCCGTCCGGCATCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGt                                                                                             <  2:132351/149‑1 (MQ=255)
                                                    ggaaaGTCTACTGGGGCTATGCGCCGAACGAGACCCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTg                                                                                            <  2:142178/147‑1 (MQ=255)
                                                    gTAAAGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCTACCATCTGGGAGCTGCTGGAAGTg                                                                                            <  1:593824/149‑1 (MQ=255)
                                                    gTAAAGTCGACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGATGAGCCGCGAAATCTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTg                                                                                            <  1:185917/149‑1 (MQ=255)
                                                       aaGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGaa                                                                                         >  1:435290/1‑149 (MQ=255)
                                                       aaGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGaa                                                                                         <  1:538825/149‑1 (MQ=255)
                                                         gTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATAAAAAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGaaaa                                                                                       <  2:632075/149‑1 (MQ=255)
                                                          tCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGaaaaa                                                                                      >  2:780182/1‑149 (MQ=255)
                                                          tCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACGACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGaaaaa                                                                                      >  2:592236/1‑149 (MQ=255)
                                                           cTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAATACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAac                                                                                     <  2:467671/149‑1 (MQ=255)
                                                             aCTGGGGCTATGCCCCCAACGAGAACCTCAGCAACGAAGAGCTGACCCGCGAAAACCACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAacac                                                                                   <  1:5222/149‑1 (MQ=255)
                                                                    cTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGGGCTGCTGGAAGTGGAAAAACACCCTGGCa                                                                            >  1:646682/1‑149 (MQ=255)
                                                                     tctGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCAt                                                                           <  2:151527/147‑1 (MQ=255)
                                                                     tATGCGCCGAACGAGACCCGCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCAt                                                                           <  2:118708/149‑1 (MQ=255)
                                                                         cgcCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACAAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCCGGAAGTGGAAAAACACACTGGCATGaaa                                                                       >  2:526589/1‑149 (MQ=255)
                                                                                   gaACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCCCAGAAt                                                             >  2:213397/1‑149 (MQ=255)
                                                                                   gaACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAAt                                                             >  2:788938/1‑149 (MQ=255)
                                                                                   gaACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGAGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCGTCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGGAt                                                             >  2:463591/1‑149 (MQ=255)
                                                                                      ccTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCtt                                                          >  2:107056/1‑149 (MQ=255)
                                                                                      ccTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCtt                                                          >  2:627087/1‑149 (MQ=255)
                                                                                          aGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGc                                                      >  1:359323/1‑149 (MQ=255)
                                                                                            cAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCa                                                    >  1:776146/1‑149 (MQ=255)
                                                                                               cGAAGAGCGGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTCGCACGAAACTCACAGAATCTTTCGCCAtgt                                                 <  2:606050/149‑1 (MQ=255)
                                                                                                     gCTGATCCGTGATAACTTCCAGGGCATCCGTCCGGGACCGGGCTATCCGGCGTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCg                                           <  1:230836/149‑1 (MQ=255)
                                                                                                        gATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATATTTCGCCATGTGGCCCGGTg                                        >  1:358466/1‑149 (MQ=255)
                                                                                                         agccGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCCGCCCGGAACATACGGAAAAAGCCACCACCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGc                                       <  1:122085/147‑1 (MQ=255)
                                                                                                          tCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCa                                      <  2:745660/149‑1 (MQ=255)
                                                                                                          cccGCGAACACTACCAGGGCACCCGGCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCa                                      <  1:635342/148‑1 (MQ=255)
                                                                                                           ccGCGAAAACCACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCAt                                     <  1:152895/149‑1 (MQ=255)
                                                                                                             gcgAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCg                                   >  2:778163/1‑149 (MQ=255)
                                                                                                              cgAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCgg                                  >  1:523356/1‑149 (MQ=255)
                                                                                                              cgAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCCGGAAGTGGAAAAACACACAGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCACCgg                                  >  2:25640/1‑149 (MQ=255)
                                                                                                                aaaaCTACCAGGGCATCCGTCCGGCACCGAGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGtt                                >  2:498771/1‑149 (MQ=255)
                                                                                                                 aaaCTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGttt                               <  1:592236/149‑1 (MQ=255)
                                                                                                                    ccgccAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCgg                            <  1:258249/146‑1 (MQ=255)
                                                                                                                     tACCAGGGCATCCGTCCGGCACCGGGCTCGCCGGCCTGCCCGGAACATACGGAAAAAGCCACCCTCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACACAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCggg                           >  1:520980/1‑149 (MQ=255)
                                                                                                                        cAGGGGATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCTGTTTg                        <  2:74199/149‑1 (MQ=255)
                                                                                                                        cAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGtgg                        >  1:592745/1‑147 (MQ=255)
                                                                                                                        cAGGGCATCCGTCCGGCACCGGGCTATCAGGCATGCTTTGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGGTg                        >  2:771993/1‑149 (MQ=255)
                                                                                                                        cAGGGCATCCGTCCGGCACCGGGCTATCAGGACTGCCAGGAACAAGATGAAAAAGCCACCATCTGGGCGTTTCTTTAAGTGGCTGATCGCGCTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTg                        <  1:386313/149‑1 (MQ=255)
                                                                                                                           gccATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAATCTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTa                     <  1:48115/147‑1 (MQ=255)
                                                                                                                               tccggccgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTc                 <  1:452281/149‑1 (MQ=255)
                                                                                                                               gccgtccgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTc                 <  1:688545/148‑1 (MQ=255)
                                                                                                                                ccgtccgGCACCGGGCAATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCa                <  2:400540/149‑1 (MQ=255)
                                                                                                                                ccgtccgGCACCGGGCAATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGACCCGCTTCCCGGTGCATCGGTTTCGGGTTGGTACTTCa                <  2:569312/149‑1 (MQ=255)
                                                                                                                                  gtccgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACCCTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGc              <  2:745062/149‑1 (MQ=255)
                                                                                                                                      ggCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCAcc          >  1:329005/1‑149 (MQ=255)
                                                                                                                                       ggaCCGTGCTATCCTGCCTGCCCGGAACAAACGGAAAACGTCACCATCTGGCAGCTGCTGGAAGTGGAAAAACTCACCGGCATGAAACTCTCAGCATCGTTCGCCTTGTGGTCCGGTGCATCGTTTTCGGGTTGGTACTTCAGCCAccc         <  2:192508/147‑1 (MQ=255)
                                                                                                                                        cACCGGGCCATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACCCTGGCATGACATTAACATTAGCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCg        <  2:171071/149‑1 (MQ=255)
                                                                                                                                          ccGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGa      <  1:497762/149‑1 (MQ=255)
                                                                                                                                            gggCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATGGTTTTCGGGTTGGTACTTCAGCGACACGGACa    >  1:69633/1‑149 (MQ=255)
                                                                                                                                             ggCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGGGGCCCGGTGCAGAGGTTTCGGGTTGGTACTTCAGCCACCCGGACAg   >  2:419227/1‑149 (MQ=255)
                                                                                                                                              gCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGCGCTGCTGGAAGTGGAAAAACACCCTGGCATGAAACTCACAGAATCTTTCGCCACGTGCCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCCGACAGc  >  2:679059/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CTTGCCGACCGTTTAGCCGAAGCCTTTGCGGAGTATCTCCATGAGCGTGTGCGTAAAGTCTACTGGGGCTATGCGCCGAACGAGAACCTCAGCAACGAAGAGCTGATCCGCGAAAACTACCAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGC  >  NC_000913/4227097‑4227387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑