Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,345,224 | C→T | 8.4% | I170I (ATC→ATT) | rpoN → | RNA polymerase, sigma 54 (sigma N) factor |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,345,224 | 0 | C | T | 8.4% | 137.1 / 3.4 | 52 | I170I (ATC→ATT) | rpoN | RNA polymerase, sigma 54 (sigma N) factor |
Reads supporting (aligned to +/- strand): ref base C (25/21); new base T (2/2); total (27/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01 |
GATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCA > NC_000913/3345078‑3345372 | gATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCACGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGatcga < 1:131944/149‑1 (MQ=255) ccTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTAGAAGATGTTGTCGAAACTATAGGCGATGAAGAGatcgacatcg > 1:100309/1‑149 (MQ=255) tGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGATTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGatcgacatcgaa > 1:583602/1‑148 (MQ=255) cgtgGCAGGCTGAGCCGACACCGTTCTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTCATACGATTGTCCCGCTGGCAGATATGCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGaa < 2:367740/148‑1 (MQ=255) aGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGCAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCCCGAAGGTATAGGCGATACAGAGATCGACACCGGCGAAGTTGaa > 2:259927/1‑149 (MQ=255) tGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCg < 1:548553/149‑1 (MQ=255) tGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGATACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAATCCGTCCtt > 2:285708/1‑149 (MQ=255) gACACCGTTTTCCGACAGTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTa < 2:129014/149‑1 (MQ=255) gACACCGTTTTCCGACACTGACCGCGCTATTTCTACCTCTAACGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTa < 2:352944/149‑1 (MQ=255) ttttCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGAt > 2:595873/1‑149 (MQ=255) ttttCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGAt > 1:91718/1‑149 (MQ=255) ttttCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGACATTCTCGAAAGTATAGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAgagaaa > 2:595887/1‑144 (MQ=255) tcccGACACTGGCCGCGCTATTGCTCCCTCTAGCGTTGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGCTGAAGAGATCGACATCGACGCAGTTGACGCCGTCCTTAAGCGGATcc < 2:660796/147‑1 (MQ=255) ctcTGGCCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGt < 1:431315/147‑1 (MQ=255) cacTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGt < 1:101450/149‑1 (MQ=255) caatGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGt < 1:732874/146‑1 (MQ=255) aCCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGAt > 2:348270/1‑149 (MQ=255) tATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCcggtcg > 2:137174/1‑149 (MQ=255) cTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCcggtcgaggtg > 2:331085/1‑144 (MQ=255) tACCTCTATCGTCGATGCCGTTCATCAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTgg < 1:671504/149‑1 (MQ=255) aCCTCTATCGTCGATGCCGTTTATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTAGAGGCGATGAAGAGATCGACATCGACGAAGTTAAAGCTGTCCTTAAGCGGATCCAAGGGTTTGGTCCGGTCGGTGTgga > 2:141811/1‑148 (MQ=255) ccTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTggcg < 1:451355/149‑1 (MQ=255) ccTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGCAGGCGCGATGAAGCGATCGACATCGACGAAGGTGAAGCCGTCCTTAAGCGTATCCAACGGTTTGATCCGGTCGGTGTggcg < 2:484478/149‑1 (MQ=255) ccTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGGCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTggcg < 1:408976/149‑1 (MQ=255) gtcgATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGGGTGGCGGCAAAAGa > 2:702196/1‑149 (MQ=255) tcgATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGAt > 2:215695/1‑149 (MQ=255) tcgATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGAt < 2:14417/149‑1 (MQ=255) tcgATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGAt > 1:78200/1‑149 (MQ=255) cGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTg > 1:437500/1‑149 (MQ=255) gTTGATGGAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGa < 2:6369/149‑1 (MQ=255) gaAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTct > 2:399518/1‑149 (MQ=255) aaaCGGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTctg > 2:628705/1‑149 (MQ=255) aaaCCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTctg > 1:686945/1‑149 (MQ=255) cgggTTATTTGACGGTCCCGCTGGAAGATATTCTCGAGAGTATCGGCGATGTAGAGATCGCCATCGAGGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTctgctg < 2:38481/147‑1 (MQ=255) ttATCTGTCTGATCCGCCGGAAGCTATTCTCGACAGTATCGGCGATGAAAAGTTCGCCATCCACGAAGTTGAAGCCGGCCTTCAGCGGATCCAACGGTTTGACCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATcc < 2:274656/149‑1 (MQ=255) aCTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACtctc > 1:759196/1‑149 (MQ=255) tGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCACCTCTccc > 1:248313/1‑149 (MQ=255) tCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGTGGCAAAAGATCTGCGTGATTGTCTGCTGATCCAACTCTCCCaa < 1:595887/149‑1 (MQ=255) tCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGTGGCAAAAGATCTGCGTGAGTGTCTGCTGATCCAACTCTCCCaa < 1:595873/149‑1 (MQ=255) tCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCaa < 2:91718/149‑1 (MQ=255) ccGCTGGAAGATATTCTCGAAAGTATAGGCGATAAAGAGAGCGACATCGACGAAGTGGAAGCCGTCCTTAAGCGGATGCAAGGGTTTGATCCGGTCGGTGTGGCGGCAAAAGAGCTGCGTGAGTGGCTGCTGATCCAAGTCTCCCAAtt > 1:399363/1‑149 (MQ=255) cgttGGAAGGTATTCTCGAAAGGATAGGCGATGAAGAGATGGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTc < 2:714147/146‑1 (MQ=255) tGGAACTTATTCTCGAAAGTATATGCCATGAAGAGATGGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGTCAAATGATCTGCGTGACTGTCTGCTGATCCAGCTCTCCCAATTCGAt < 2:157201/149‑1 (MQ=255) gAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGCCTGTCTGCTGATCCCACTCTCCCAATTCGATaa > 2:110945/1‑149 (MQ=255) gAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCGGCTGATCCAACACTCCCAATTCGATaa > 1:689912/1‑149 (MQ=255) atatTCTCGAAATTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGAcc < 1:499737/149‑1 (MQ=255) gAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCGAACTCTCCCAATTCGATAAGACCACGCCGTg > 1:538113/1‑149 (MQ=255) aaaGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTgg < 1:710272/149‑1 (MQ=255) aaaGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGAACCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGAGCCTACTCTCCCAATTCGATAAGACCACGCCGTgg > 2:639200/1‑149 (MQ=255) aaaGGATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTTCACAGCGGGAGCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTgg < 1:621332/149‑1 (MQ=255) gcgcTGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGCTACGACCACGCCGTGGCTGGCagag < 2:590275/149‑1 (MQ=255) cGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAgg > 1:331527/1‑149 (MQ=255) cGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAgg > 2:797255/1‑149 (MQ=255) tGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGGGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCa > 1:227542/1‑149 (MQ=255) agaTCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTg > 1:642079/1‑149 (MQ=255) atcgacatcgacGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGGTAAGACCACGCCGTGGCTGGAAGCGGCCAGACTAAt > 1:2154/1‑149 (MQ=255) atcgacatcgacGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGAt > 1:180349/1‑149 (MQ=255) cgatatcgacGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCa > 2:99825/1‑149 (MQ=255) cgacatcgacGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCa > 2:99841/1‑149 (MQ=255) | GATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCA > NC_000913/3345078‑3345372 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |