Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 819,918 A→G 9.2% Q12R (CAA→CGA)  ybhL → putative acetate transporter; BAX Inhibitor‑1 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913819,9180AG 9.2% 94.2 / 3.7 44Q12R (CAA→CGA) ybhLputative acetate transporter; BAX Inhibitor‑1 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (23/16);  new base G (2/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-02

ACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCG  >  NC_000913/819770‑820064
                                                                                                                                                    |                                                                                                                                                  
aCCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACa                                                                                                                                                    >  1:143596/1‑149 (MQ=255)
  cGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACGGATTCCCACGTTCTGATCCAATCGTACAAc                                                                                                                                                  >  2:93692/1‑149 (MQ=255)
  cGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGCTTCAATCGcacacc                                                                                                                                                  >  2:135625/1‑147 (MQ=255)
   gCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTATAAcc                                                                                                                                                 >  2:718933/1‑149 (MQ=255)
    cTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAAccc                                                                                                                                                >  2:521166/1‑149 (MQ=255)
           cGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGggctg                                                                                                                                         >  1:147120/1‑149 (MQ=255)
            ggCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACGACCCCGggctgg                                                                                                                                        >  1:298425/1‑149 (MQ=255)
            ggCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACGACCCCGggctgg                                                                                                                                        >  1:493295/1‑149 (MQ=255)
                    cACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCaaa                                                                                                                                <  1:521166/149‑1 (MQ=255)
                              tACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCACACTTATATAGc                                                                                                                      >  2:708612/1‑149 (MQ=255)
                              tACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGc                                                                                                                      <  1:719556/149‑1 (MQ=255)
                                 aTACGTTTACGCTGCGTCTATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCa                                                                                                                   >  1:587942/1‑149 (MQ=255)
                                     gTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTc                                                                                                               >  1:68309/1‑149 (MQ=255)
                                       ttACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACGACCCCGGGCTGGCTTGCAAACTTATATGGCTCACGTCtg                                                                                                             <  2:298425/149‑2 (MQ=255)
                                       ttACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACGACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTa                                                                                                             <  2:493295/149‑1 (MQ=255)
                                            cTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGAGCGTCATGGACAGATTCCCACGTTCAGATTCAATCGTACAACCCCGGGCTGGCTTGAAACCTGATTTAGTTCAATTCTTTTGCt                                                                                                        >  2:354280/1‑149 (MQ=255)
                                                 tCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATg                                                                                                   >  2:735912/1‑149 (MQ=255)
                                                       cacaGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGtt                                                                                             >  1:776628/1‑149 (MQ=255)
                                                         caGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGGTGACCGTTgg                                                                                           >  1:399174/1‑149 (MQ=255)
                                                             cTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGGTGGATGACCGTTGGCttg                                                                                       >  1:361251/1‑149 (MQ=255)
                                                                       tGCAGTATCCTTAAGAGATTCCGTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCg                                                                             <  2:324560/149‑1 (MQ=255)
                                                                          aGTATCCTTAAGATCTTCCTTATATCTTCAGGAGATTGTCATTGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCATACTTATATGGCTCAAGTCTATGGCTGGATGACCCTTGGCTTCTTGCTGACCGCAt                                                                          <  1:565583/149‑1 (MQ=255)
                                                                                 ttAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGGTTCAATCGTACAGCCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGCTCACCGTTGGGGTGTTTCTGACGCCGATTGTTGc                                                                   >  1:141838/1‑149 (MQ=255)
                                                                                 ttAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGCTGACCGTTGGCTTGTTGCTGACCGCATTTGTTGc                                                                   >  1:345817/1‑149 (MQ=255)
                                                                                 ttAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTACTGATACAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGc                                                                   >  2:248506/1‑149 (MQ=255)
                                                                                 tgaaGATATTCCTTATGTCTGCAGGGGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCGTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGc                                                                   <  1:36964/147‑1 (MQ=255)
                                                                                    aGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTg                                                                <  1:653460/149‑1 (MQ=255)
                                                                                     gATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTgg                                                               >  1:759967/1‑149 (MQ=255)
                                                                                       tatTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTa                                                             <  1:658156/149‑1 (MQ=255)
                                                                                         ttCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCCTTTGTTGCCTggggtg                                                           >  2:206197/1‑145 (MQ=255)
                                                                                           cgttATATGTTCAGGAGATCGTCATCGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTGTATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCg                                                         <  1:484750/147‑1 (MQ=255)
                                                                                            cTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCgg                                                        <  2:143596/149‑1 (MQ=255)
                                                                                                 atCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAAt                                                   <  1:656990/149‑1 (MQ=255)
                                                                                                     tCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCcg                                               >  2:89761/1‑149 (MQ=255)
                                                                                                        ggAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCgg                                            >  2:165248/1‑149 (MQ=255)
                                                                                                                tCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATg                                    >  1:38065/1‑149 (MQ=255)
                                                                                                                    ggACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGTTGGAGc                                >  2:791452/1‑149 (MQ=255)
                                                                                                                             cccACGTTCTGGTTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCa                       <  2:8850/149‑1 (MQ=255)
                                                                                                                               cACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCGCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACt                     >  1:423933/1‑149 (MQ=255)
                                                                                                                                      tcattcattcGTACACCCCCGGGCTGGCTTCCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTCTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCgtg              <  2:477984/147‑1 (MQ=255)
                                                                                                                                       gATGCAATCGTACCACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCgtgt             <  1:45522/149‑1 (MQ=255)
                                                                                                                                              tCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGGGCTGTTGTTCTCTAACCGTGTCTTTTTa      >  1:342580/1‑149 (MQ=255)
                                                                                                                                                 tACACCCCCGGGCTGGCTTGCAACCTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATc   <  2:179305/149‑1 (MQ=255)
                                                                                                                                                 tACACCCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCTTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATc   <  1:531236/149‑1 (MQ=255)
                                                                                                                                                 caCAACCCCCGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATc   <  1:140580/148‑1 (MQ=255)
                                                                                                                                                  aCAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCg  <  1:727989/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCG  >  NC_000913/819770‑820064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑