Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 435,396 T→A 11.2% I87N (ATT→AAT)  nusB → transcription antitermination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913435,3960TA11.2% 108.0 / 8.9 45I87N (ATT→AAT) nusBtranscription antitermination protein
Reads supporting (aligned to +/- strand):  ref base T (17/23);  new base A (2/3);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-02

GGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCC  >  NC_000913/435250‑435542
                                                                                                                                                  |                                                                                                                                                  
ggtagTAAAAGACGTTGACTGCCTGTACTTCCGTGCGCTGCCTGCCGGGGTGGCGACTAATACCGCATACCTCGACGTACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTg                                                                                                                                                  <  1:174069/145‑1 (MQ=255)
  aTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGTACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTgcg                                                                                                                                                <  1:518650/149‑1 (MQ=255)
  aTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCCGGCCGGGGTGGCGACTAAGACCGCATACCGCGACGGACTGATGACGCCATACCTGTCCCGTCCGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTgcg                                                                                                                                                <  1:241836/149‑1 (MQ=255)
        acgACGTTGCCGTCTGGTACTTCCGTGAGCGGCTGGCCGGGGTGGCGACTTATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGATAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTAc                                                                                                                                          <  2:203710/147‑1 (MQ=255)
        aaGACGTTGACGGCCTGTACGTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATTCCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTAc                                                                                                                                          <  2:102662/149‑1 (MQ=255)
          gACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGa                                                                                                                                        <  2:730170/149‑1 (MQ=255)
           aCGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGACAAAGCAGTACAGCGCAATGCGCAGTActgc                                                                                                                                       <  1:417527/149‑4 (MQ=255)
           aCGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACAGCGCAATGCGCAGTActgc                                                                                                                                       <  2:65333/149‑4 (MQ=255)
           aCGCTGACGTTCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACAGCGCAATGCGCAGTActgc                                                                                                                                       <  2:741909/149‑4 (MQ=255)
              ttGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTg                                                                                                                                    >  1:650268/1‑149 (MQ=255)
                        gTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTa                                                                                                                          <  1:759626/149‑1 (MQ=255)
                          aCTTCCGTGAGCTGCTGGCCGGGATGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGAAGc                                                                                                                        >  1:757955/1‑149 (MQ=255)
                                              ggggTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGGCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGc                                                                                                    <  1:417990/149‑1 (MQ=255)
                                                ggTGGCTACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCa                                                                                                  <  1:597528/149‑1 (MQ=255)
                                                ggTGGCGACCAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCa                                                                                                  <  1:628191/149‑1 (MQ=255)
                                                    gCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTaa                                                                                              >  2:533002/1‑149 (MQ=255)
                                                      gACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCGGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACg                                                                                            >  2:195577/1‑149 (MQ=255)
                                                          aaTACCGCAGACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGc                                                                                        <  1:146770/149‑1 (MQ=255)
                                                           aTACCGCATACCTCGACGGACTGATGAAGCCATCCCTGTCCCGCCTGCTGGAAGAACTGGGACATGTAGAAACAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCCACAACGAAGCg                                                                                       >  2:721713/1‑149 (MQ=255)
                                                                     ccTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTgg                                                                             >  2:784053/1‑149 (MQ=255)
                                                                      cTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGc                                                                            >  2:448369/1‑149 (MQ=255)
                                                                      cTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGc                                                                            >  1:256083/1‑149 (MQ=255)
                                                                         gACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGaa                                                                         >  2:778371/1‑149 (MQ=255)
                                                                             gACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTTGCCATTAACGAAGCGATCGAACTGGCGAAATCg                                                                     <  1:510812/149‑1 (MQ=255)
                                                                             gACTGATGAAGCCATACCTGTCCCGCCGGCTGGAAGAACTGGGACAGGAACAAAAGGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCg                                                                     <  1:579528/149‑1 (MQ=255)
                                                                                tgatgaAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAACGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCAATGTGCCATACAAAGTGGCCATTTACGAAGCGATCGAACTGGCGAAATCGTTc                                                                  <  2:439814/149‑1 (MQ=255)
                                                                                tgatgaAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTc                                                                  <  2:399874/149‑1 (MQ=255)
                                                                                  atgaAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCgg                                                                >  1:730760/1‑149 (MQ=255)
                                                                                   tgaAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGgc                                                               <  1:784053/149‑1 (MQ=255)
                                                                                    gacgCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGGAGTACTGCGCATTGCGCTGTACGAACTGTCTAAGCGTAGCGATGTGGCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGccg                                                              <  2:141623/146‑1 (MQ=255)
                                                                                                         gctgGAACAACTGGGACAGGTAGAAAACGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCg                                         <  1:501083/149‑1 (MQ=255)
                                                                                                                   cggggACAGGTAGAAAAAGCAGTACTGCGCATTGCGCGGGACGAACTGTCTAAACGTAGCGATGTGCCATGCAAAGTGGCCAGGAACGAAGCGAGCGAACCGACGAAAGCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCCACGGCGt                               >  2:203050/3‑149 (MQ=255)
                                                                                                                      ggACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACt                            >  1:56292/1‑149 (MQ=255)
                                                                                                                       gACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGCAATCGTTCGGCGCAGAAGACAGCCATCAGTTCGTCACCGGCGTACTc                           >  1:616533/1‑149 (MQ=255)
                                                                                                                       gACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTtctc                           >  1:603205/1‑149 (MQ=255)
                                                                                                                       gACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTc                           <  2:434858/149‑1 (MQ=255)
                                                                                                                       gACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTc                           >  2:529917/1‑149 (MQ=255)
                                                                                                                              aGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGCCAGCGATAAGTTCGTCAACGGCGTACTCGATAAag                    <  1:195577/149‑1 (MQ=255)
                                                                                                                                 aaaaGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAagcag                 >  2:172326/1‑149 (MQ=255)
                                                                                                                                  aaaGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAagcagc                >  1:796516/1‑149 (MQ=255)
                                                                                                                                    gttcgTACAGCGCAATGCGCAGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCAc              >  1:339667/5‑149 (MQ=255)
                                                                                                                                     ttcgTACAGCGCAATGCGCAGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCAcc             >  1:117359/4‑149 (MQ=255)
                                                                                                                                         tACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCtgtg         <  2:650268/149‑1 (MQ=255)
                                                                                                                                                cATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGcc  <  1:726854/149‑1 (MQ=255)
                                                                                                                                                cATTGCGCTGTACAAACTGCCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAAGCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGcc  <  1:373026/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCC  >  NC_000913/435250‑435542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑