| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 126,947 | G→T | 100% | R418L (CGT→CTT) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 126,947 | 0 | G | T | 100.0% | 100.3 / NA | 33 | R418L (CGT→CTT) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (22/11); total (22/11) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTC > NC_000913/126802‑127080 | aaaCGTGCAGAAGCAGCCCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTaa < 2:234139/149‑1 (MQ=255) aaCGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGGATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAAc > 1:732044/1‑149 (MQ=255) gCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGGCTTAACTGGGTAGTGGTCCCGCATGTTAGTccgttg > 1:230264/1‑144 (MQ=255) ggtATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACaa > 1:106828/1‑149 (MQ=255) ggtATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACaa > 1:276726/1‑149 (MQ=255) aTCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGGAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAAc > 1:158121/1‑149 (MQ=255) aTCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAAc < 2:16409/149‑1 (MQ=255) gCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATGGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTCCTCACTTAGACAAACCAGCTATc > 2:154797/1‑149 (MQ=255) gCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATc > 2:777819/1‑149 (MQ=255) tgctgcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTGACTTCGACAAAACCGATATCAcc > 2:259173/1‑149 (MQ=255) tgctgcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATACCGCATGTTACTCACTTCGACAAAACCGATATCAcc > 2:259158/1‑149 (MQ=255) gcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGACATCGAAGAAGTGGAACTGGGCCGCAAACAGAAAAACTCTGGTGCGAACATGAGTCTTAACTGGGTAATGATGCCGCATGTTACTCACTTCGACAAAACCGATATGACCGAGt > 2:70952/1‑149 (MQ=255) gTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTAGACAAAACCGATATCACCGAGTTgg > 1:71715/1‑149 (MQ=255) cGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATCTCACCGAGTTGGagccg > 1:178055/1‑145 (MQ=255) cGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCg > 2:525429/1‑149 (MQ=255) ggTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTcc < 1:790927/149‑1 (MQ=255) ttCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGCGTTGGAAGCGTTCCGTAAAca > 2:419498/1‑149 (MQ=255) gcAGCAAGTTTGGTAAAATCGAAGAAGTGGAAGTGGGCCGCATCCAGAAAACCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAAcag < 1:212676/148‑1 (MQ=255) aaGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGaa < 1:737622/149‑1 (MQ=255) ggggAAATCGAAGAAGTGGCCCTGGCCCGCCTCCACACAATCTCGAGTGCCACCATGATGCTTAGCTGGGTTAGGAGCCGGCAGGGTACTCACTTCGACAAATCCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACgaaga < 2:666975/146‑1 (MQ=255) ggTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGCGTTGGAAGCGTTCCGTAAACAGCAGAACgaaga > 2:301895/1‑149 (MQ=255) aaTCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAgcgg > 2:501466/1‑149 (MQ=255) aTCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCGGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAgcggc < 1:140278/149‑1 (MQ=255) cGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAGACAGCAGAACGAAGAAGCAGCGa > 1:318411/1‑149 (MQ=255) aagaagTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGaaa > 1:641246/1‑149 (MQ=255) ggAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGc < 2:125934/149‑1 (MQ=255) ggAACGGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGc < 2:64180/149‑1 (MQ=255) tGGGCCGCCTCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGAt < 1:650893/149‑1 (MQ=255) gggCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGACTTAACTGGGTAATGATCCCGCATGTTAGTCACTTAGACAAAACGGATATCACCGAGTTGGAAGCGTTCCGTGAACAGCAGAACGAAGAAGCGGGGAAACGTAAGCTGGAtg > 2:415818/1‑149 (MQ=255) cATCCAGAAAATCTCTGGTGCGAACCTGGGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGa < 2:244922/149‑1 (MQ=255) cATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAgg > 1:118160/1‑148 (MQ=255) aGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACCGCAGAACGAAGAAGCGGCGAAACGTCAGCTGCACGTGAAGCGCAcc > 2:31544/1‑149 (MQ=255) gTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCtgc > 2:417834/1‑147 (MQ=255) | AAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTC > NC_000913/126802‑127080 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |