Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913	1,978,503	Δ776 bp	100%		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1978503	1979278	776	36 [0]	[1] 37	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1978502	0 (0.000)	31 (0.730)	24/260	NT	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

ATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCT  >  NC_000913/1978359‑1978645                                                                                                                                                |                                                                                                                                                aTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataa                                                                                                                                            <  1:277652‑M1/149‑6 (MQ=255) aTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataa                                                                                                                                            <  1:560928‑M1/149‑6 (MQ=255)   gggTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGGGGGGGGCGGCGATTTTCAATAATGCGTGATGCAAATCACCCAAAACACTCAATGACCTAACATAAATGtataagt                                                                                                                                          >  2:191377‑M1/1‑142 (MQ=255)     gTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtca                                                                                                                                        >  1:200637‑M1/1‑140 (MQ=255)     gTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGAGGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtca                                                                                                                                        >  1:64018‑M1/1‑140 (MQ=255)                   cTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGATGGGGGCTGCGATTTTCAATAATGCGTGATGCACATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                          <  2:532928‑M1/149‑24 (MQ=255)                   cTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                          <  2:63596‑M1/149‑24 (MQ=255)                   cTAAGATTTGTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                          <  2:796701‑M1/149‑24 (MQ=255)                         tttctCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATTCAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAATCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtat                                                                                                                    <  2:322771‑M1/149‑30 (MQ=255)                          ttttCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatt                                                                                                                   >  1:301970‑M1/1‑119 (MQ=255)                                         gCAACTGTACGCGCCCCTACACGCACATACAACGGACGGGGGCTGCGATTTTCCATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaa                                                                                                    <  1:32874‑M1/149‑46 (MQ=255)                                          cAACTGTACTCGTCACTACACGCACATACAACGGAGGGGTGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaag                                                                                                   <  1:448560‑M1/149‑47 (MQ=255)                                           aaCTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagt                                                                                                  >  1:140835‑M1/1‑102 (MQ=255)                                             cTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCCATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttataaagttt                                                                                                >  2:368814‑M1/1‑100 (MQ=255)                                              tGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtaattcaatctgttaaaaagtttt                                                                                               >  2:752254‑M1/1‑99 (MQ=255)                                                         cTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactttttttttgggtgtatttcaatctgttaaaaaatttttcgctacgata                                                                                    >  1:1957‑M1/1‑88 (MQ=255)                                                         cTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgcta                                                                                    >  1:797179‑M1/1‑88 (MQ=255)                                                          tACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctag                                                                                   >  1:160074‑M1/1‑87 (MQ=255)                                                             acGCACATACAACGGACGGGGGCTGCGATTTTCAATAATGCGTAACGCAGATCACACAAAACTCTCTATTACTTAACATACATGtataagacatacttttgttttgggtgtatttcattctgttaaaaagtttttcgctacgctagcaa                                                                                <  2:277543‑M1/149‑66 (MQ=255)                                                                       aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACAATCAATTACTTAACATAAATGtataagtcatacttttgttttgagtgtatttcaatctgttaaaaagtttttcgctacgctaccaagcaaaaatga                                                                      >  2:442078‑M1/1‑74 (MQ=255)                                                                              gggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagga                                                               <  1:339504‑M1/149‑83 (MQ=255)                                                                              gggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagga                                                               <  1:461992‑M1/149‑83 (MQ=255)                                                                              gggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagga                                                               <  2:200637‑M1/149‑83 (MQ=255)                                                                              gggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAAGTACTTAACATAGATGtataagtcattcttttgttttgggtgtatttcaatctgttaaaaagtttttcgtttcgatagaaagaaaaaatgaaacagga                                                               <  2:252830‑M1/149‑83 (MQ=255)                                                                                   cTGCGATTTTCAATAATGCGTGATGCAGATCAGACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtcttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacgggaataat                                                          <  2:446589‑M1/149‑88 (MQ=255)                                                                                    tGCTATTTTCAATAATGCGTAATGCATATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatc                                                         <  2:201450‑M1/149‑89 (MQ=255)                                                                                    tGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTCCTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatc                                                         >  2:363405‑M1/1‑61 (MQ=255)                                                                                                taatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGaataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaacatgaaacaggaataatcgaaatgggatgt                                             >  2:522460‑M1/1‑49 (MQ=255)                                                                                                  atGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctggcaagcaaaaatgaaacaggaataatcgaaatgggatgttg                                           >  2:210909‑M1/1‑47 (MQ=255)                                                                                                      gTGATGCAAATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctatcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgca                                       <  1:394960‑M1/149‑107 (MQ=255)                                                                                                           gCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtc                                  <  1:34804‑M1/149‑112 (MQ=255)                                                                                                           gCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttaggtgtctttcaatatgttaaaaaggtttttggttcgctcgcaagcaataatgaaacaggaataatagaaatgggatgttgcgcccagtg                                  >  2:685358‑M1/1‑38 (MQ=255)                                                                                                             aGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaa                                <  2:301970‑M1/149‑114 (MQ=255)                                                                                                                                     ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatc        >  2:537712‑M1/1‑12 (MQ=255)                                                                                                                                      tAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatct       <  2:709062‑M1/149‑139 (MQ=255)                                                                                                                                           tAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctat  <  2:160074‑M1/149‑144 (MQ=255)                                                                                                                                                |                                                                                                                                                ATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCT  >  NC_000913/1978359‑1978645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑