Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,413,542 A→T Y67N (TAT→AAT)  ydaC ← DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,413,5420AT98.2% 531.5 / NA 163Y67N (TAT→AAT) ydaCDUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (82/78);  minor base G (3/0);  total (85/78)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

CGAAGCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTC  >  NC_000913/1413397‑1413687
                                                                                                                                                 |                                                                                                                                                 
cGAAGCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCaa                                                                                                                                                >  2:1176796/1‑149 (MQ=255)
   aGCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGc                                                                                                                                             <  1:950859/149‑1 (MQ=255)
    gCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCa                                                                                                                                            >  1:2101471/1‑149 (MQ=255)
     cATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCaa                                                                                                                                           <  2:1649862/149‑1 (MQ=255)
      aTCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGGTTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAAt                                                                                                                                          >  2:1467713/1‑149 (MQ=255)
      aTCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAAt                                                                                                                                          >  1:1046471/1‑149 (MQ=255)
      aTCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTACCATCGCATCACCTCAGGCCGGCGCCAATGTTAGACTGAAATTGACGCCCGCTGTTGATTATTAATAATCAGCTATGAAGGTTTAATTTGAATGCAATGAAAt                                                                                                                                          >  2:508871/1‑149 (MQ=255)
              gTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAgg                                                                                                                                  >  1:158939/1‑149 (MQ=255)
                aTTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGAc                                                                                                                                >  2:838642/1‑149 (MQ=255)
                    aaCCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTGTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGCTGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGaa                                                                                                                            >  2:2261722/1‑149 (MQ=255)
                    aaCCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGaa                                                                                                                            >  2:1620492/1‑149 (MQ=255)
                    aaCCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGaa                                                                                                                            <  2:1450312/149‑1 (MQ=255)
                    aaCCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGaa                                                                                                                            <  1:1713104/149‑1 (MQ=255)
                        aTCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGttt                                                                                                                        >  2:285168/1‑149 (MQ=255)
                         tCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTtc                                                                                                                       <  1:1301042/149‑1 (MQ=255)
                           cACCCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGGTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTtctc                                                                                                                     <  1:1820456/149‑1 (MQ=255)
                              tctTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCa                                                                                                                  >  1:2482217/1‑149 (MQ=255)
                              tctTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTCTACATTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCa                                                                                                                  >  1:2815658/1‑149 (MQ=255)
                                    ttAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGATATGAAGTTATACTTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTaaa                                                                                                            <  1:724440/149‑1 (MQ=255)
                                     tAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGAAGCCGGATTTTGATTATAAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTaaaa                                                                                                           >  1:1469593/1‑149 (MQ=255)
                                      aaaGATTACTTGTGCCATCGCATCACCTGCGCATGAAGTCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGCTTTAATTTGAATTCAATGCAATTCCCGAGGACTGAAGTTACTCGCATTTAAAAt                                                                                                          >  1:735452/1‑149 (MQ=255)
                                      aaaGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAAt                                                                                                          <  2:140339/149‑1 (MQ=255)
                                       aaGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCTAGGGCTGAAGTTTCTCGCCATTAAAAtt                                                                                                         >  2:539853/1‑149 (MQ=255)
                                        aGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAGTTTTCTCGCACTTAAAAttt                                                                                                        >  2:109491/1‑149 (MQ=255)
                                          actACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTAt                                                                                                      <  1:1228839/147‑1 (MQ=255)
                                          aTTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTGGATTTCATGGTAATTCTCGAGGACTGAAGTTTCTGGCAATTAAAATTTAt                                                                                                      >  2:644641/1‑149 (MQ=255)
                                               ttGGGCCATCGCATCACCTCTGGCGGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGtt                                                                                                 <  2:2689609/149‑1 (MQ=255)
                                                 gtgCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTAAAATTTATCAGtttt                                                                                               <  2:2151253/149‑1 (MQ=255)
                                                 gtgCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGtttt                                                                                               >  1:1499823/1‑149 (MQ=255)
                                                   gCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTAc                                                                                             >  1:2917324/1‑149 (MQ=255)
                                                     cATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACtt                                                                                           >  1:2606223/1‑149 (MQ=255)
                                                     cATCGCATCACCCCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACtt                                                                                           <  1:101006/149‑1 (MQ=255)
                                                         gCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTccg                                                                                       <  2:2787928/149‑3 (MQ=255)
                                                         gCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCtt                                                                                       >  1:2515133/1‑148 (MQ=255)
                                                         gCACCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCGCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTg                                                                                       <  1:537223/149‑1 (MQ=255)
                                                           aTCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATCAAAATTTATCAGTTTTACTTTCTGct                                                                                     >  2:2703058/1‑149 (MQ=255)
                                                            tCACCTCTGGTAGGCGCCAATGTTCGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGctc                                                                                    <  1:1378820/149‑1 (MQ=255)
                                                            tCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGaa                                                                                                                            <  2:2457175/109‑1 (MQ=255)
                                                             cACCTCTGGCAGGCTCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGctct                                                                                   <  1:104515/149‑1 (MQ=255)
                                                              aCCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGctctc                                                                                  >  2:1690823/1‑149 (MQ=255)
                                                              aCCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGctctc                                                                                  <  2:2101471/149‑1 (MQ=255)
                                                               ccTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGctctct                                                                                 <  2:1202325/149‑1 (MQ=255)
                                                               ccTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGctctct                                                                                 <  1:491514/149‑1 (MQ=255)
                                                                ctctGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTg                                                                                >  2:2928702/1‑149 (MQ=255)
                                                                ctctGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTg                                                                                >  1:2419367/1‑149 (MQ=255)
                                                                 tccggCAGGCGCAAACGTCAGACTAAAAATTACGCCCGATGTTGATTATTAATAATCAGCTATGCAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTgg                                                                               <  1:901691/146‑1 (MQ=255)
                                                                   tGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAGTTCAATGCAATTCTCGCGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGaa                                                                             >  2:2971324/1‑149 (MQ=255)
                                                                       aGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTAt                                                                                                      >  1:2196617/1‑120 (MQ=255)
                                                                       aGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGACACGc                                                                         >  2:63481/1‑149 (MQ=255)
                                                                         gcgcCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCt                                                                       <  1:373500/149‑1 (MQ=255)
                                                                         gcgcCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCt                                                                       >  1:166801/1‑149 (MQ=255)
                                                                          cgcCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGCAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTg                                                                      <  1:1003071/149‑1 (MQ=255)
                                                                           gcCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTgg                                                                               <  2:396454/139‑1 (MQ=255)
                                                                             cAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCCCTGGAAACGCCTGctt                                                                   >  1:489611/1‑149 (MQ=255)
                                                                             cAATGTTAGACTGAAATTGACGCCCGATATTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGctt                                                                   >  1:1980025/1‑149 (MQ=255)
                                                                                 gTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGTACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCttt                                                               <  1:1493621/149‑1 (MQ=255)
                                                                                 gTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGTACTGAAGCTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCttt                                                               <  2:1553713/149‑1 (MQ=255)
                                                                                  ttAGACGAAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTCAAGTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCtttt                                                              <  1:708264/149‑1 (MQ=255)
                                                                                  ttAGAATGAAATTGACGCCAGCGTTTTATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCtttt                                                              <  2:1516098/149‑1 (MQ=255)
                                                                                   tAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGaa                                                                             >  2:2823461/1‑133 (MQ=255)
                                                                                   tAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGaa                                                                             <  1:2823461/133‑1 (MQ=255)
                                                                                   tAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCCCTATGGACACGCCTCCTTCttttt                                                             <  2:2991471/149‑1 (MQ=255)
                                                                                     gACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCCATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTa                                                           >  1:597999/1‑149 (MQ=255)
                                                                                        tGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAAGGCCTGCTTCTTTTTTACCt                                                        >  1:588268/1‑149 (MQ=255)
                                                                                        tGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCt                                                        >  1:1463511/1‑149 (MQ=255)
                                                                                           aaTTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAGTTTATCAGTATTACTTTCTGCTCGCTGGAAACGCCTGCTTCTTTTTTACCTgag                                                     >  1:2661396/1‑149 (MQ=255)
                                                                                            cttGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATGCAATGCAATTCTCGAGGACGGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTgaga                                                    >  1:617107/2‑149 (MQ=255)
                                                                                            aTTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTgaga                                                    <  1:1138745/149‑1 (MQ=255)
                                                                                                   cccGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCAtttt                                             >  2:2374516/1‑149 (MQ=255)
                                                                                                    ccGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCAttttt                                            >  1:268265/1‑149 (MQ=255)
                                                                                                    ccGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTACTCGCAATTAAAATTTATCAGTATTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCAttttt                                            >  2:1240616/1‑149 (MQ=255)
                                                                                                            gATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCGTTc                                    >  2:2699199/1‑149 (MQ=255)
                                                                                                            gATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTc                                    >  2:2236032/1‑149 (MQ=255)
                                                                                                             attattAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGttt                                                                                                                        >  2:1955771/1‑64 (MQ=255)
                                                                                                             attattAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCCGTTTCACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCCTTCt                                   >  1:832435/1‑149 (MQ=255)
                                                                                                             attattAATAATCAGCTATGAAGTTTTAATTTCAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCt                                   <  2:2321850/149‑1 (MQ=255)
                                                                                                              ttattaATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTg                                  <  1:1883377/149‑1 (MQ=255)
                                                                                                              ttattaATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTg                                  >  1:236058/1‑149 (MQ=255)
                                                                                                                  tattaatCAGCTATGAAGTTTTCATTTGAATTCCATCCAATAATTCAGTATTGGAGTTTCTCGCAACAAAAAGTCATAAGTGGCACATTATGCGCTCGGGTAACGCTTGCTTATTTGTTACCTGAGAGCATTTTTTCGCATGCTGAttt                              >  2:1358501/4‑149 (MQ=255)
                                                                                                                  taataaTCAGCTATGAAGTTTTATTTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGCttt                              >  2:2073748/1‑149 (MQ=255)
                                                                                                                  taataaTCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGAttt                              <  2:1289523/149‑1 (MQ=255)
                                                                                                                    ataatCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCg                            <  1:184449/149‑1 (MQ=255)
                                                                                                                      aatCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACTTGAGAGCATTTTTTCGCATTCTGATTTCGtt                          >  2:1614322/1‑149 (MQ=255)
                                                                                                                       atCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTa                         <  1:1356209/149‑1 (MQ=255)
                                                                                                                        tCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTACGATAg                        <  2:1133530/149‑1 (MQ=255)
                                                                                                                          aGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGGGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGtt                      <  1:285168/149‑1 (MQ=255)
                                                                                                                          aGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGtt                      <  1:614162/149‑1 (MQ=255)
                                                                                                                          aGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGtt                      >  1:2430419/1‑149 (MQ=255)
                                                                                                                                   gTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGCGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTg             <  1:2656110/149‑1 (MQ=255)
                                                                                                                                    ttttAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAAGGCCTTCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGa            >  1:1461920/1‑149 (MQ=255)
                                                                                                                                    ttttAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCCGATTTCGTTAGTTTAGATTTTGa            <  1:904157/149‑1 (MQ=255)
                                                                                                                                         atgtGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATc       <  1:1134724/146‑1 (MQ=255)
                                                                                                                                         aTTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATc       >  1:2262980/1‑149 (MQ=255)
                                                                                                                                         aTTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATc       <  2:1463511/149‑1 (MQ=255)
                                                                                                                                           ttGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCtt     <  1:2050864/149‑1 (MQ=255)
                                                                                                                                           ttGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACATGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAAGATCtt     >  2:1673083/1‑149 (MQ=255)
                                                                                                                                            tGAATTCAATGCAATTCTCGGGGTCTGAAGTTTCTCGCAATTAAAATTTATTAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTg    <  2:1342717/149‑1 (MQ=255)
                                                                                                                                             gAATTCAATGCACTTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGt   <  2:1857457/149‑1 (MQ=255)
                                                                                                                                              aaTTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGGGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTc  <  2:1138103/149‑1 (MQ=255)
                                                                                                                                              aaTTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTc  <  2:158939/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CGAAGCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTC  >  NC_000913/1413397‑1413687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: