Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258674 258683 10–776 83 [0] [0] 83 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)77 (1.200) 58/266 0.1 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers

TGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCG  >  NC_000913/257766‑258048
                                                                                                                                             |                                                                                                                                             
tgtCAAAAATTTGCTAACTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGTGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAa                                                                                                                                              <  2:416983‑M1/143‑2 (MQ=255)
tggtaaaAATCTTCTAGATTTTCCCAATGTCGTACAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACCCCCGAATAGCAGATTGATCAAAAAATTTACCGCCCTAGGCCCGTATATTCGTGAAGGTAa                                                                                                                                              <  2:1186365‑M1/140‑2 (MQ=255)
  ttAAAAATTGGCTAAATGTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagt                                                                                                                                            <  1:1011685‑M1/143‑4 (MQ=255)
             cTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataat                                                                                                                                 <  1:157607‑M1/143‑15 (MQ=255)
              tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatc                                                                                                                                >  1:130796‑M1/1‑128 (MQ=255)
              tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataagg                                                                                                                                >  1:257757‑M1/1‑128 (MQ=255)
                aaTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcga                                                                                                                              >  2:901951‑M1/1‑126 (MQ=255)
                aaTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAGATTCGTGAAGGTAagtgcaaagataatcga                                                                                                                              >  2:1116264‑M1/1‑126 (MQ=255)
                     ggcCAATGTGGTAAAACAGGTGCAGCACGACAGGAGATAGCAATGACGTTACCGAGGGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATAGTCGCGAAGGTAagtgcaaagataatcgattctt                                                                                                                         <  2:654560‑M1/142‑23 (MQ=255)
                      gCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTCTATTCGTGAAGGTAcgtgcaaagataatcgcttcttt                                                                                                                        >  2:965369‑M1/1‑120 (MQ=255)
                             tGGTAAGACAGTGGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATCCGTGAAGGTAagtgcaaagataatcgattctttttcgatt                                                                                                                 <  2:807736‑M1/143‑31 (MQ=255)
                              ggTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattg                                                                                                                >  2:481254‑M1/1‑112 (MQ=255)
                                tAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTACATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtc                                                                                                              >  1:1113660‑M1/1‑110 (MQ=255)
                                tAAAACAGTGGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtc                                                                                                              <  2:773089‑M1/143‑34 (MQ=255)
                                  aaaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctg                                                                                                            >  1:1158874‑M1/1‑108 (MQ=255)
                                   aaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtggaaagataatcgattcttgttcgattgtctgg                                                                                                           >  1:956605‑M1/1‑107 (MQ=255)
                                       gTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgt                                                                                                       >  1:645876‑M1/1‑103 (MQ=255)
                                        ttGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgta                                                                                                      >  1:662295‑M1/1‑102 (MQ=255)
                                            aTCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGCTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcgttttcgattggctggctgtatgcg                                                                                                  >  1:1098387‑M1/1‑98 (MQ=255)
                                             tCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgatggtctggctgtatgcgt                                                                                                 >  2:5168‑M1/1‑97 (MQ=255)
                                                caacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaa                                                                                              >  2:80893‑M1/1‑94 (MQ=255)
                                                 aacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac                                                                                             >  2:1029673‑M1/1‑93 (MQ=255)
                                                 aacaGGAGATAGAAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac                                                                                             <  1:1003582‑M1/143‑51 (MQ=255)
                                                     ggAGACAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCGCGTATAGTCGTGTAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtga                                                                                         <  2:842392‑M1/143‑55 (MQ=255)
                                                         aTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacc                                                                                     >  1:626260‑M1/1‑85 (MQ=255)
                                                           atcaaTGACGTCACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagatatccgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccgg                                                                                   <  2:361596‑M1/141‑61 (MQ=255)
                                                           aGCAAGGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttgttcgattgtctggctgtatgcgtcaacgtgaaaccgg                                                                                   <  2:427819‑M1/143‑61 (MQ=255)
                                                                   cGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGGGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcccaggaa                                                                           >  2:674883‑M1/1‑75 (MQ=255)
                                                                     ttACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagatagtcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagt                                                                         >  2:166510‑M1/1‑73 (MQ=255)
                                                                     ttACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagt                                                                         >  2:252385‑M1/1‑73 (MQ=255)
                                                                     ttACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagt                                                                         <  1:73807‑M1/143‑71 (MQ=255)
                                                                       aCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTCCCGCCCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgc                                                                       <  1:380346‑M1/143‑73 (MQ=255)
                                                                        ccGAGTGGACACCCGAGGGGCAGATTGATCAAAAAATTTACCGCACTAGGCCGGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttgcgattgtctggctgtatgcggcaacgtgaaaccggcaccggaagtgcg                                                                      <  2:1022974‑M1/143‑74 (MQ=255)
                                                                         cGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgt                                                                     <  1:929823‑M1/143‑75 (MQ=255)
                                                                         cGAGTAGACACCAGAAGAGAAGATTGATCAAAAAATTTACCGTACTAGGCCCGTATATTCGTGAAGGTAagtgaaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgt                                                                     <  1:120579‑M1/143‑75 (MQ=255)
                                                                                 cacGCGAAGAGCAGATTGGTCAAGAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg                                                             <  2:676624‑M1/143‑83 (MQ=255)
                                                                                    ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgggg                                                          >  1:935692‑M1/1‑58 (MQ=255)
                                                                                     cGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaacggcaccggaagtgcgtgaattctggggc                                                         >  2:262491‑M1/1‑57 (MQ=255)
                                                                                     cGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtacaactataatctcttctttttcgattgtctggctgtatgcgtccacgtgaacccgccacccgaagtgcctgacgtctcgggc                                                         >  1:445577‑M1/1‑57 (MQ=255)
                                                                                         gagCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgagtctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggt                                                     <  2:472105‑M1/143‑91 (MQ=255)
                                                                                          ggcAGGTTGATCAAAAAAGTTACCGCACTAGGCCCGTATATTCGTGCAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtg                                                    <  2:192345‑M1/142‑92 (MQ=255)
                                                                                          agCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtacaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtg                                                    <  1:1035514‑M1/143‑92 (MQ=255)
                                                                                          agCAGATTCTGCAACAAACTTACCGCACGAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctgtttcgattggcgggctgtatgcgtcaacgtgaacccggcaccggaagtgcgtgaattctgggcctgttg                                                    <  2:442953‑M1/143‑92 (MQ=255)
                                                                                           gCAGATTTATCAAAAAATTTACCGCACTAGGCCCGTAGATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgg                                                   <  1:990109‑M1/143‑93 (MQ=255)
                                                                                           gCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaagtctggggctggtgg                                                   >  2:563458‑M1/1‑51 (MQ=255)
                                                                                              gATTGATCAAAAAATTTACCGCACTAGGCCCGTATAGTCGTGAATGTAagggcaaagatagtagagtctgtttcggttgtctggctgtatgcgtccacgtgaaaccggcaccggaagggcgtgagttctgggtctgggggttg                                                >  1:167874‑M1/1‑48 (MQ=255)
                                                                                                 tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAAGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatcgag                                             >  2:1186621‑M1/1‑45 (MQ=255)
                                                                                                   aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgcttgtctggctgtatgcgtcaacgtgaaaccggcaccggacgtgcgtgaattctggggctgtggcatggcgcc                                           >  2:898072‑M1/1‑43 (MQ=255)
                                                                                                   aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtctgcgcgtacggggaaccggccccggtagtgcgtgaattctggggacggtggacggacca                                           >  1:991208‑M1/1‑43 (MQ=255)
                                                                                                      aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccgggagtgcgtgaattctggggctggtggatggagctgga                                        >  1:823739‑M1/1‑40 (MQ=255)
                                                                                                      aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggcgctgga                                        >  2:165539‑M1/1‑40 (MQ=255)
                                                                                                      aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgga                                        >  2:1079449‑M1/1‑40 (MQ=255)
                                                                                                      aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgga                                        >  1:974296‑M1/1‑40 (MQ=255)
                                                                                                       aaaaaggtcccgcactcggcCCGTATATTCGTTATGTTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaa                                       <  2:736281‑M1/134‑105 (MQ=255)
                                                                                                       aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaa                                       <  1:1029673‑M1/143‑105 (MQ=255)
                                                                                                       aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaa                                       >  2:807557‑M1/1‑39 (MQ=255)
                                                                                                       aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataaccgattctttttcgatggtctggctgtatgcgtcaacgtgaaaccggcaccggaagtccgtgaattctggggctggtgggtggagctggca                                       >  2:213905‑M1/1‑39 (MQ=255)
                                                                                                         aaaTTCACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctggatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagc                                     <  1:718509‑M1/143‑107 (MQ=255)
                                                                                                           tttgaccgcTCCAGGCCCTTATATGCGTGAAGTTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattatggggctggtggatggagctggaagcgc                                   <  2:722936‑M1/139‑109 (MQ=255)
                                                                                                            tttACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                  <  1:481254‑M1/143‑110 (MQ=255)
                                                                                                             ttACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                 <  1:653985‑M1/143‑111 (MQ=255)
                                                                                                                 cGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgagttctggggctggtggatggcgcaggaagcgcaggaat                             >  1:359161‑M1/1‑29 (MQ=255)
                                                                                                                    tctttgcccgtATATTCTTGTAGGTAagttcaaatataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatccc                          <  2:970661‑M1/138‑118 (MQ=255)
                                                                                                                    aCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggcggtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggagggagctggaagcgcaagaacccc                          >  2:591132‑M1/1‑26 (MQ=255)
                                                                                                                     cTAGGCCCGTATATTCGTGAAGGTAggtgcaaagataatcgattctgtttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccg                         <  2:892950‑M1/143‑119 (MQ=255)
                                                                                                                         gCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaacccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtttt                     <  2:445790‑M1/143‑123 (MQ=255)
                                                                                                                         gCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgccggaatcccgtttt                     >  2:177816‑M1/1‑21 (MQ=255)
                                                                                                                         gCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtttt                     >  1:652274‑M1/1‑21 (MQ=255)
                                                                                                                          cccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaatgctggggctggtggatggaggtggaagcggaggaagcccgtttta                    >  2:760453‑M1/1‑20 (MQ=255)
                                                                                                                           ccGTATATTCGTGAAGGTAagtgcaaagataatcgcttctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgcatggagctggaagcgcaggaatcccgttttgc                   >  2:1077101‑M1/1‑19 (MQ=255)
                                                                                                                           ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggcagcccgttttac                   >  1:101822‑M1/1‑19 (MQ=255)
                                                                                                                           ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttac                   <  1:1094953‑M1/143‑125 (MQ=255)
                                                                                                                              tataTTCGTGAAGGTAagtgcaaagataatcgattctttttcggttgtctggctgtatgcgacaacgtgaaaacggcaccggaagtgcgtgaattctggggctggtggatggagctggaagagcaggaatccggcgttaggta                >  2:696540‑M1/1‑16 (MQ=255)
                                                                                                                                tatTCGTGAAGGTAagggcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagggcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctaca              <  1:901951‑M1/143‑130 (MQ=255)
                                                                                                                                 atTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggcgctggaa                                       >  2:506637‑M1/1‑13 (MQ=255)
                                                                                                                                 atTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaa                                       <  1:506637‑M1/117‑105 (MQ=255)
                                                                                                                                  ttCGTGAAGGTAagtgcaaagataatcgagtctgtttcgagtgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagt            <  2:130796‑M1/143‑132 (MQ=255)
                                                                                                                                       gAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgggaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttacca       <  1:1063933‑M1/143‑137 (MQ=255)
                                                                                                                                        aaGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttactag      >  1:850977‑M1/1‑6 (MQ=255)
                                                                                                                                           gTAggtgcaaggataatcgattctttttggattgtcttgctgtatgcgtcaacgtgaaaccggcagcgggagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagttt   <  2:1113660‑M1/143‑141 (MQ=255)
                                                                                                                                           gTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagttt   <  2:174807‑M1/143‑141 (MQ=255)
                                                                                                                                            tAagtgcacagctactcgattctctttcgattgtctggctgtatgcgtcaacgcgaatccggcaccggaagtgcgtgaattcttgggctggtggatggagctggaatcgcaggaatcccgttttacctacagttaccagtttg  <  2:1098387‑M1/143‑142 (MQ=255)
                                                                                                                                            tAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttg  <  1:1096726‑M1/143‑142 (MQ=255)
                                                                                                                                             |                                                                                                                                             
TGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCG  >  NC_000913/257766‑258048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: