Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 417,753 Δ1 bp 100% coding (612/690 nt) phoB → response regulator in two‑component regulatory system with PhoR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913417,7510A.63.1% 60.2 / 221.1 111coding (610/690 nt)phoBresponse regulator in two‑component regulatory system with PhoR
Reads supporting (aligned to +/- strand):  ref base A (17/24);  new base . (30/40);  total (47/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 112 total aligned reads displayed.

TCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAA  >  NC_000913/417463‑418034
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                           
tggCCGAAGGGGCTGGTGGCGCGAATCAAAGCGGTAATGCGGCGTATTTCGCCAATGGCCGTAGAGGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACAGCTCACCGAGTGATGGTGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTa                                                                                                                                                                                                                                                                                             <  2:180307/287‑1 (MQ=255)
           gCTGGTTGGGCGAAGAAAAGCGGTAATGCGCCGTAGTTCTCCGATGGCGGTGGAAGGAGGGATTGTGAGCAAGGGAGTAAGTATCGACCCGACATCTCACCGAGTGAGGGCGGGCGAAGAGCCGCTGGAGATGGGGCCTACAGAATTTAAGCTGCTGCACTTTTTTATGACGCATCCTGTGCGCGTGTAGAGCCGCTAGCAGCTGTTAAACCACGTCTGGAGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAg                                                                                                                                                                                                                                                                                  <  2:568491/288‑1 (MQ=255)
                      gAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCa                                                                                                                                                                                                                                                                       <  1:970133/289‑1 (MQ=255)
                       aaTGAAAACGGTAATGCGCCGTATTTCGCCAATGGCGGGGGAAGAGGGGATTGAGGTGCAGGGATTAAGTCGCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGGGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCa                                                                                                                                                                                                                                                                       <  2:597797/288‑1 (MQ=255)
                            aaGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGc                                                                                                                                                                                                                                                                <  1:254523/289‑1 (MQ=255)
                            aaGCGGTAATGCGCCGTATTACGCCAAGGGGGGTGGAAGGGGGGATTGAGATGCACGGATTAAGTCTGGAACCGACATATCAGCGAGTGAGGGCGGGCGAAGAGCCGCTGGAGGTGGGGCCGAAAGAATTTCAACTGCTGCACGTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCAACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGc                                                                                                                                                                                                                                                                <  2:847167/289‑1 (MQ=255)
                            aaGCAGTAATGCGCCCTATTTCGCCAATGGCGGGGGAGTAGGTGATGGAGATGCCGGGATTAAGTCTACACCCGACAACTCACCGGGTGATGGCGGGCGAAGAGCCGCTGGGTATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGTAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGc                                                                                                                                                                                                                                                                <  2:433944/289‑1 (MQ=255)
                             aGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAGGAGGGGATTGGGATGCAGGGATTAAGGCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGCGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCa                                                                                                                                                                                                                                                               <  2:331472/289‑1 (MQ=255)
                             aGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCa                                                                                                                                                                                                                                                               <  1:768966/289‑1 (MQ=255)
                               cGGTAATGGGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCa                                                                                                                                                                                                                                                               <  2:195192/288‑1 (MQ=255)
                               cGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGGGGTGATTGAGATGCAGGGATTAAGTGTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCAt                                                                                                                                                                                                                                                              <  2:494078/288‑1 (MQ=255)
                               cGGTAAGGCGCCGTATTTCGCCAATGGGGGTGGGAGGGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCAt                                                                                                                                                                                                                                                              <  2:169707/288‑1 (MQ=255)
                                ggTAATGGGCCGTATTTCGCCAATGGGGGTGGAAGAGGTGATTGAGAGGCAGGGATTGAGTCTCGACCCGACATCTAACCGAGTGATGGCGGGCGAAGGGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCAt                                                                                                                                                                                                                                                              <  2:919921/288‑1 (MQ=255)
                                ggTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCa                                                                                                                                                                                                                                                               <  1:648280/287‑1 (MQ=255)
                                ggTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCa                                                                                                                                                                                                                                                               <  1:171713/287‑1 (MQ=255)
                                ggTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCAt                                                                                                                                                                                                                                                              <  1:457484/287‑1 (MQ=255)
                                       cgcCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGc                                                                                                                                                                                                                                                                <  2:439841/279‑1 (MQ=255)
                                       cgcCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGc                                                                                                                                                                                                                                                                >  1:439841/1‑279 (MQ=255)
                                       cgcCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGa                                                                                                                                                                                                                                                      >  1:191135/1‑288 (MQ=255)
                                            tATTTCGCCACTGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGCGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTCTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTACAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGt                                                                                                                                                                                                                                                  >  1:909606/1‑288 (MQ=255)
                                            tATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGt                                                                                                                                                                                                                                                  >  1:448891/1‑288 (MQ=255)
                                            tATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGGGTATGTGGAAGCCCGCACGGTCGCTGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgc                                                                                                                                                                                                                                                >  2:715258/1‑289 (MQ=255)
                                             aTTTGGCCAGTGGCGGTGGAATAGGTGATTGAGATGCAGGGATTAAGTCTCCACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGGGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgcg                                                                                                                                                                                                                                               <  1:216480/289‑1 (MQ=255)
                                             aTTTCGCCAATGGCGGTGGAAGAGGTGATTGGGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgcg                                                                                                                                                                                                                                               <  2:136568/289‑1 (MQ=255)
                                              tttCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGCGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgcg                                                                                                                                                                                                                                               <  1:509058/289‑1 (MQ=255)
                                                      aTGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCAt                                                                                                                                                                                                                                                              >  1:950421/1‑265 (MQ=255)
                                                      aTGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCAt                                                                                                                                                                                                                                                              <  2:950421/265‑1 (MQ=255)
                                                      aTGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgcg                                                                                                                                                                                                                                               <  2:799000/280‑1 (MQ=255)
                                                      aTGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgcg                                                                                                                                                                                                                                               >  1:799000/1‑280 (MQ=255)
                                                      aTGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACAGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGGCCGGTAcgg                                                                                                                                                                                                                                       >  2:220090/1‑287 (MQ=255)
                                                             tGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCg                                                                                                                                                                                                                                <  1:230094/288‑1 (MQ=255)
                                                                   agGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGGACAGGATATCGTTTTTCa                                                                                                                                                                                                                         >  1:668133/1‑289 (MQ=255)
                                                                     gTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCaa                                                                                                                                                                                                                        >  1:640386/1‑288 (MQ=255)
                                                                       gATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgcg                                                                                                                                                                                                                                               <  2:283312/264‑1 (MQ=255)
                                                                       gATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgcg                                                                                                                                                                                                                                               >  1:283312/1‑264 (MQ=255)
                                                                                     gATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGcc                                                                                                                                                                                                         <  1:962642/288‑1 (MQ=255)
                                                                                       ttAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGc                                                                                                                                                                                                     <  2:558222/289‑1 (MQ=255)
                                                                                        tAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGc                                                                                                                                                                                                     <  2:448891/289‑1 (MQ=255)
                                                                                        tAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGc                                                                                                                                                                                                     <  1:914221/289‑1 (MQ=255)
                                                                                         aaGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGcct                                                                                                                                                                                                                                                              >  2:329790/1‑228 (MQ=255)
                                                                                         aaGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCAt                                                                                                                                                                                                                                                              <  1:329790/230‑1 (MQ=255)
                                                                                                  cccGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACg                                                                                                                                                                                           <  1:921497/288‑1 (MQ=255)
                                                                                                  acctaaaTCTCAACGAGTGATGGCGGTCGGAGAGCGGCTGCGGATGGGGCCGACAGAATTTACACGGCTGCACTATTTTATGTCGCAGCCTGAGCGCGGGTACAGCCGCGAGCAGCTGTTAAACGACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGTGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTGTTCAACCCGCTTTTAACGCCTTGCTCATCGGACg                                                                                                                                                                                           <  2:679983/282‑1 (MQ=255)
                                                                                                      aCATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCaga                                                                                                                                                                                       >  1:788449/1‑289 (MQ=255)
                                                                                                      aCATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCaga                                                                                                                                                                                       >  1:776100/1‑288 (MQ=255)
                                                                                                       cATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCagag                                                                                                                                                                                      <  1:121314/289‑1 (MQ=255)
                                                                                                       cATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGc                                                                                                                                                                                     <  2:254821/289‑1 (MQ=255)
                                                                                                            cACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgcg                                                                                                                                                                                                                                               <  1:632107/226‑1 (MQ=255)
                                                                                                            cACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgcg                                                                                                                                                                                                                                               >  2:632107/1‑226 (MQ=255)
                                                                                                            cACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAggg                                                                                                                                                                                 >  1:210339/1‑288 (MQ=255)
                                                                                                                 aGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgcg                                                                                                                                                                                                                                               <  1:739332/222‑1 (MQ=255)
                                                                                                                 aGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgcg                                                                                                                                                                                                                                               >  2:739332/1‑222 (MQ=255)
                                                                                                                 aGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGACCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCCTGACCGCCAGGTTCAGACCGTCTGCGGTACCGGCTATCGTTTTTCCATCCGCTTCTAACGCCTTGCTCCTTGGACGCAGATCCGGGCTTAt                                                                                                                                                                            >  2:977857/1‑289 (MQ=255)
                                                                                                                    gttGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCCTCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACAACGCATGGGGAACTAACGTGTATGTGGAAGACAGCACGGGCGATGTCCCCATTCGTCGCCTGCGTCAGCACTGGAGCCCGGCGGGCATGACAGCACGGTGCAGTCCGTGGGCGGTCCAGGACATCGTTTTTCACTCCGCTTTTAACGCCTTTCTCACCGGACGCTGAGGAGGGCtgaagctt                                                                                                                                                                        >  2:729554/3‑282 (MQ=255)
                                                                                                                     aTGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGCCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGAtt                                                                                                                                                                        >  1:279423/1‑288 (MQ=255)
                                                                                                                      tGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGAttt                                                                                                                                                                       <  2:580675/288‑1 (MQ=255)
                                                                                                                       ggcgggcgAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCt                                                                                                                                                                     >  1:495234/1‑289 (MQ=255)
                                                                                                                            gcgAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTaa                                                                                                                                                                  <  1:47354/288‑1 (MQ=255)
                                                                                                                             cgAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGAACGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTCTTTCAACCCGATTTTAACGCATTGCTCATCGCACGCTGAGCAGGGCTTCTGAGTTCTTacatg                                                                                                                                                               >  1:463982/1‑285 (MQ=255)
                                                                                                                               aaGAGCGGCTGGAGTATGGGCCGACAGAATTTAAACTGGTGCACTTTTTTAAGCAGCATCCTGGGCGCGTGTACAACCGCGAGCAGCTGGTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGGCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGa                                                                                                                                                             <  2:536281/289‑1 (MQ=255)
                                                                                                                                          gaagaggggACGGCAGAAATTAAACGGCTGAAGTGTGTTATGAAGCCTCCGTAGCGCGTGTTCAGCGTCGAGCAGCTGTTAAACCACGTCTGGGGAGCTGAAGGGTCTGGGGAAGACCGCACGGTGGCTGTCCACATTCGTCGCCTGTGTAAGCACAGGAGCCCGGCGGGCATGACAGCATGGTGGAGACCGTGCGGGGTATAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGGGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACg                                                                                                                                                   <  2:849400/283‑1 (MQ=255)
                                                                                                                                            gaTGGGGCCGACGGAATTTAAACTTCGTCACTTTTTTATGACGCATCCTGGGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTg                                                                                                                                                 <  2:438220/288‑1 (MQ=255)
                                                                                                                                              tGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCt                                                                                                                                               <  1:64353/288‑1 (MQ=255)
                                                                                                                                                    cGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGGGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACg                                                                                                                                         >  1:286230/1‑289 (MQ=255)
                                                                                                                                                               aaaCTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGaa                                                                                                                             <  2:210339/289‑1 (MQ=255)
                                                                                                                                                                    gctccACTTTTTTATGAGGCATCCTGGACGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGc                                                                                                                        <  2:495234/289‑1 (MQ=255)
                                                                                                                                                                      tgcactttgtagtggcgaacctcgatcgcgTGTACAGCGCCGGGCAGCTGTGAAACACCGTCTGGGGACCTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGc                                                                                                                        <  2:461612/262‑1 (MQ=255)
                                                                                                                                                                      tgcACTTTTTTATGACGCAGCCGGAGGGGGTGTACAGCCGCGAGGAACTGTTAAACCACGTCTGGCGGACTAGCGTGTATGTGGAAGACCGCACGGTCGATGTCCACGTTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTGTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGc                                                                                                                        <  2:717635/288‑1 (MQ=255)
                                                                                                                                                                               ttATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGa                                                                                                              >  1:386143/1‑289 (MQ=255)
                                                                                                                                                                                              aGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCtt                                                                                                                                                      <  2:65755/234‑1 (MQ=255)
                                                                                                                                                                                              aGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCtt                                                                                                                                                      >  1:65755/1‑234 (MQ=255)
                                                                                                                                                                                              aGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTtgctg                                                                                               >  1:85289/1‑288 (MQ=255)
                                                                                                                                                                                                    tgtACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCCGTCGTGGAAAAGGCTGGTGCCGGAGCTGCTCCTTTGCTGCCTcc                                                                                          >  2:657902/1‑288 (MQ=255)
                                                                                                                                                                                                    tgtACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCCTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTTCTGGGGCTGCTACTTTGCTGCCttc                                                                                          >  2:998855/1‑286 (MQ=255)
                                                                                                                                                                                                    tgtACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCt                                                                                                                 >  1:384683/1‑264 (MQ=255)
                                                                                                                                                                                                    tgtACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCt                                                                                                                 <  2:384683/264‑1 (MQ=255)
                                                                                                                                                                                                    tgtACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCg                                                                                        >  1:258526/1‑289 (MQ=255)
                                                                                                                                                                                                       aCAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCgg                                                                                       <  1:220090/288‑1 (MQ=255)
                                                                                                                                                                                                             gcgAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGGGCCGCTACTTTCATTCCTCCCGGCTTTCac                                                                                >  2:558591/1‑287 (MQ=255)
                                                                                                                                                                                                                         tAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCAtt                                                                    <  1:816152/288‑1 (MQ=255)
                                                                                                                                                                                                                          aaaCCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCAttt                                                                   >  2:267169/1‑289 (MQ=255)
                                                                                                                                                                                                                                cGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGtt                                                            >  1:881818/1‑289 (MQ=255)
                                                                                                                                                                                                                                   cTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTAc                                                          <  2:191135/288‑1 (MQ=255)
                                                                                                                                                                                                                                        gAACTAACGGGGATGTGGAAGACCGCACGGTCGATGTCCACATTCGGCGCCTGGGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGc                                                      <  2:589699/288‑1 (MQ=255)
                                                                                                                                                                                                                                                   tATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTa                                                                                                                                                                                                              >  1:787965/1‑124 (MQ=255)
                                                                                                                                                                                                                                                   tATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTa                                                                                                                                                                                                              <  2:787965/124‑1 (MQ=255)
                                                                                                                                                                                                                                                       tgGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGc                                      <  2:776100/288‑1 (MQ=255)
                                                                                                                                                                                                                                                        ggAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACCTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCa                                     >  2:337965/1‑288 (MQ=255)
                                                                                                                                                                                                                                                         gAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCa                                     <  1:657902/288‑1 (MQ=255)
                                                                                                                                                                                                                                                           aGACCGGCCGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTGTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCgg                                 <  2:884708/289‑1 (MQ=255)
                                                                                                                                                                                                                                                             aCCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGt                                >  2:255011/1‑289 (MQ=255)
                                                                                                                                                                                                                                                               cGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCCTCCTGGTGGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCCTCGGTAAc                             >  2:328935/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                 cACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCg                                                                                        >  1:225864/1‑228 (MQ=255)
                                                                                                                                                                                                                                                                 cACGGTCGATGTCCACATTCGTCGCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCg                                                                                        <  2:225864/228‑1 (MQ=255)
                                                                                                                                                                                                                                                                         aTGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCt                    >  1:263527/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                caTTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTg              >  1:680658/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                    cgtcgCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGGACAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTAGCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTGGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCAttt        <  2:379112/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      tcgCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACTGGACTGCTTATCTGGCAtt         >  1:123151/1‑286 (MQ=255)
                                                                                                                                                                                                                                                                                      tcgCCTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCAtt         >  1:258368/1‑286 (MQ=255)
                                                                                                                                                                                                                                                                                          cTGCGTAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGACAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGaa  >  2:726184/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                           
TCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAACGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAA  >  NC_000913/417463‑418034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: