Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	417,368	Δ5 bp	coding (227‑231/690 nt)	phoB →	response regulator in two‑component regulatory system with PhoR

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	417368	417372	5	41 [0]	[0] 41	phoB	response regulator in two‑component regulatory system with PhoR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 417367	0 (0.000)	41 (0.970)	36/284	0.2	100%	coding (226/690 nt)	phoB	response regulator in two‑component regulatory system with PhoR
	?	NC_000913	417373 =	0 (0.000)					coding (232/690 nt)	phoB	response regulator in two‑component regulatory system with PhoR

CGGTCGAAGCGGAAGATTATGACAGTGCTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCG  >  NC_000913/417227‑417506                                                                                                                                              |                                                                                                                                            cGGTCGAAGCGGAAGATTATGACAGTGCTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGCTGTT‑CCCTCGCGCCACCGGTATCCAGTTCACCAAACACCTCAAGCGCGACTCCGTGACCCGGGcc                                                                                                                                           >  1:256208‑M1/1‑141 (MQ=255)    tCGAAGCGGAAGATTATGACAGTGCTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCCGTTCATCAAACACCTCAAGCGCGAGTCGAAGACCCGGGcccgg                                                                                                                                        >  1:347359‑M1/1‑138 (MQ=255)       aaGCGGAAGATTATGACAGTGCTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggt                                                                                                                                     <  1:48850‑M1/143‑9 (MQ=255)          cGGAAGATTATGACAGTGCTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGCTGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgat                                                                                                                                  <  2:660960‑M1/143‑12 (MQ=255)            gAAGATTATGACAGTGCTGTGAATCAACTGAATGAACCCTGGCCGGATTTACTTCTCCTCGACTGGATGTT‑ACCGGGCGGCTCCGGTATCCAGTTCATCAGACACCTCAAGCGCGAGTCGATGCCCCGAGccagtgatgcagc                                                                                                                                >  1:147189‑M1/1‑130 (MQ=255)            gAAGATTATGACAGTGCTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgctgt                                                                                                                                >  2:482897‑M1/1‑130 (MQ=255)            gAAGATTATGACAGTGCTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgt                                                                                                                                <  2:439975‑M1/143‑14 (MQ=255)             aaGATCATGACAGTGCTGCGAATCAACTGAAGGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTACCCAGTTCATCAATCACCGCAAGCGCGAGTCGATGACCCGGGccagtggtgatgtt                                                                                                                               <  2:139640‑M1/143‑15 (MQ=255)              aGATTATGACAGTGCTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccggtggtgatgttg                                                                                                                              >  2:38726‑M1/1‑128 (MQ=255)                     gACAGTGCTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgcca                                                                                                                       <  2:418978‑M1/143‑23 (MQ=255)                          tGCTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagaggg                                                                                                                  <  1:560634‑M1/143‑28 (MQ=255)                            cTGTGAATCAACTGAATGACCCCTGGCCTTATTTAATCCCCCTCGACTGGAGGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagagggga                                                                                                                <  2:456338‑M1/143‑30 (MQ=255)                              gtgAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGAGGACCCGGGccagtggtgatgttgacggccagaggggaag                                                                                                              >  2:481609‑M1/1‑112 (MQ=255)                                     aaCTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTGTCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGCCCCGGGcaggacgtggtgtggacggcccgggggggcgaagaaga                                                                                                       >  2:567415‑M1/1‑105 (MQ=255)                                       gtgggtgagccctgGCCGGATTGAGTTCTCCTCGAGGGGATGTT‑ACCTGGCGGCGCCGGTATCCAGGTCATCAAACGCCTCAAGCGCGAGTCGAGGAGCCGGGccagtggtgatgttgaccgccagaggggaagaagaagatc                                                                                                     <  1:196999‑M1/138‑41 (MQ=255)                                          aaggggccctggccgGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcg                                                                                                  <  2:317967‑M1/137‑44 (MQ=255)                                             gtaccCTGGCCGGGTTTAACTCTCCTCGACTGG‑TGTTGACGTGGAGTCTCGGGTATCCAGTTCATCAACCACCTCCAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcgtgc                                                                                               <  1:631095‑M1/141‑47 (MQ=255)                                                 ccTGGCCGGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgtggcccgccagaggggaagaagacgagcgcgtgcgcgg                                                                                           >  2:69205‑M1/1‑93 (MQ=255)                                                  cTGGCCGGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagaggtgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggc                                                                                          >  1:525619‑M1/1‑92 (MQ=255)                                                   tGGCCGGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggcgatgttgacggccagaggggaagcagacgaccgcgggcgcggcc                                                                                         >  1:407958‑M1/1‑91 (MQ=255)                                                           tttAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaacc                                                                                 >  2:491310‑M1/1‑83 (MQ=255)                                                                      tcGCCTGGACGTG‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatga                                                                      <  1:175245‑M1/143‑72 (MQ=255)                                                                      tcGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatga                                                                      >  1:197346‑M1/1‑72 (MQ=255)                                                                      tcGACTGGATGGT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatga                                                                      >  2:619304‑M1/1‑72 (MQ=255)                                                                         aCTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatgacta                                                                   <  1:561015‑M1/143‑75 (MQ=255)                                                                                gTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgagtagggcgacttgaaaccggcgcggatgactatatcacc                                                            >  2:689704‑M1/1‑62 (MQ=255)                                                                                  t‑ACCTGGCGGCTCCGGTATCCATTTCATCAAACTCCTCAAACGCGAGTCGATGACCCGGGccagtgttgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatgactatatcaccaa                                                          <  2:136649‑M1/143‑84 (MQ=255)                                                                                             cTCCGGGATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatgactatatcaccaagccgttttcg                                                <  1:660019‑M1/143‑94 (MQ=255)                                                                                                cGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatgactatatcaccaagccgttttcgccg                                             >  1:666419‑M1/1‑47 (MQ=255)                                                                                                  gTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatgactatatcaccaagccgttttcgccgaa                                           <  2:494174‑M1/143‑99 (MQ=255)                                                                                                         gTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatgactatatcaccaagccgtgtgcgccgaaggagcgg                                    >  2:725120‑M1/1‑38 (MQ=255)                                                                                                          ttCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGccagtggtaatgttgaccgacaggcgggcagaagaagatcgcgtgcgcggacttgaaagcggcgcggatgactacagcaccaagccgttgtcgccgagaccgctgg                                   >  1:676418‑M1/1‑37 (MQ=255)                                                                                                                 aaCACCTCAAGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatgactatatcaccaagccgttttcgccgacggagctggtggcgcg                            >  2:668282‑M1/1‑30 (MQ=255)                                                                                                                        ccagcGCGAGACGGTGACCCCGGccagggttgacgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatgactatatcaccaagccgttttcgccgaaggagctggtggcgcgaatcaaa                     <  2:165507‑M1/141‑121 (MQ=255)                                                                                                                        caggcgcgggCCGAGGACCCGGGccagtggggatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatgactatatcaccaagccgttttcgccgaaggagctggtggcgcgaatcaaa                     <  2:256208‑M1/140‑121 (MQ=255)                                                                                                                        cAAGCGCGAGTAGAGGACCCTGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatgactatatcaccaagccgttttcgccgaaggagctggtggcgcgaatcaaa                     <  1:605175‑M1/143‑121 (MQ=255)                                                                                                                          aGCGCGAGTCGATGACCCGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatgactatatcaccaagccgttttcgccgaaggagctggtggcgcgaatccaagc                   >  2:93003‑M1/1‑21 (MQ=255)                                                                                                                          aGCGCGAGTCGATGACCCGGGccagtggtgatgtagaccgccagaggggaagaagaagatcgagtgggcggccttgaaaacggcgcggaggacgatatcaccaagccgttttagccgaaggggctggtggcgggaatcaaagc                   >  1:33027‑M1/1‑21 (MQ=255)                                                                                                                                    gATGCCCCGGGccagtggtgatgttggccgccaggggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatgactatatcaccaagccgttttcgccgaaggagctggtggcgcgaatcaaagcggtaatgcgc         <  1:156149‑M1/143‑133 (MQ=255)                                                                                                                                          ccGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatgactatatcaccaagccgttttcgccgaaggagctggtggcgcgaatcaaagcggtaatgcgcagtagt   >  1:625871‑M1/1‑5 (MQ=255)                                                                                                                                           cGGGccagtggtgatgttgaccgccagaggggaagaagaagatcgcgtgcgcggccttgaaaccggcgcggatgactatatcaccaagccgttttcgccgaaggagctggtggcgcgaatcaaagcggtaatgcgccggattt  >  2:587796‑M1/1‑4 (MQ=255)                                                                                                                                              |                                                                                                                                            CGGTCGAAGCGGAAGATTATGACAGTGCTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTT‑ACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCG  >  NC_000913/417227‑417506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑