Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,103,655 Δ1 bp coding (1320/1374 nt) cpxA ← sensory histidine kinase in two‑component regulatory system with CpxR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,103,6530G.98.4% 269.9 / ‑3.4 62coding (1322/1374 nt)cpxAsensory histidine kinase in two‑component regulatory system with CpxR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (27/34);  minor base C (1/0);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGA  >  NC_000913/4103511‑4103790
                                                                                                                                              |                                                                                                                                         
ccTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTCTACAGCGGCAACCAAATCACCAGCCCTCACCCGCCCAGCg                                                                                                                                           >  1:843374/1‑143 (MQ=255)
   gATAAGACGCTATCAGCGTCGCATCAGGTATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCggct                                                                                                                                        <  2:452863/143‑3 (MQ=255)
    aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCcgcgcctg                                                                                                                                       >  2:900505/1‑139 (MQ=255)
       aGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGtct                                                                                                                                   <  1:19217/143‑1 (MQ=255)
         aCGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTAAAGTCAAGTTCGAAGTTTAACCCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGtcttc                                                                                                                                 <  2:1092917/143‑1 (MQ=37)
           tcTATCTGCGTCGCTTCAGGCATCCTGCCCAAGTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTGCAAATGCGAAGCTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTg                                                                                                                               <  1:643467/142‑1 (MQ=37)
             tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGCTGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAACTCACCCGCCGTACACCGCCCCGCGGCCGCCTTCTGcc                                                                                                                             >  2:301015/1‑143 (MQ=37)
             tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGcc                                                                                                                             >  1:543494/1‑143 (MQ=255)
             tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGcc                                                                                                                             <  1:516349/143‑1 (MQ=255)
              aTCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCt                                                                                                                            >  2:178804/1‑143 (MQ=255)
               tCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCtt                                                                                                                           <  2:18558/143‑1 (MQ=255)
               tCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCtt                                                                                                                           >  2:1098557/1‑143 (MQ=255)
                    gTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCcccc                                                                                                                      >  2:883540/1‑143 (MQ=255)
                    gTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCAccc                                                                                                                      <  1:1004822/143‑1 (MQ=255)
                       gCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGc                                                                                                                   <  2:861168/143‑1 (MQ=255)
                            aGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCCGCGGCTGTCTTCTGCCTTCACCCAGCCACGa                                                                                                              >  2:79585/1‑143 (MQ=255)
                            aGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGa                                                                                                              <  2:71808/143‑1 (MQ=255)
                            aGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGa                                                                                                              <  2:610847/143‑1 (MQ=255)
                              gCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATCCAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTCCTGCCTTCACCCAGCCACGAtg                                                                                                            >  1:839811/1‑143 (MQ=255)
                                   cTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATgctgct                                                                                                       <  2:835848/143‑1 (MQ=255)
                                    tGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGctgctg                                                                                                      >  2:1026463/1‑143 (MQ=255)
                                     gCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGa                                                                                                     >  2:82700/1‑143 (MQ=255)
                                     gCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGa                                                                                                     <  2:845438/143‑1 (MQ=255)
                                     gCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCACTTATTCAGCGACAACCAAATCACCAGACGTAAAATCATCACCGGCTATCATCTGCCTTCACCACGCCACTATACTGCTGa                                                                                                     >  1:650688/1‑143 (MQ=21)
                                        cAAACGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATg                                                                                                  <  2:978756/143‑1 (MQ=255)
                                          aaTGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGc                                                                                                >  1:306943/1‑143 (MQ=255)
                                               cGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTACCTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGttt                                                                                           <  1:346389/143‑1 (MQ=255)
                                               cGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATGCAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGttt                                                                                           <  1:299062/143‑1 (MQ=255)
                                                ggATGGGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCAGatcggaagagcg                                                                                          <  1:415630/143‑12 (MQ=255)
                                                  atccggcgTCACCGCCTTATCCCGCCTGCAAATGCGACGTTTACCCCCGCTTCTACAGCGGCAACCAACTCCCCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTcaa                                                                                        <  2:62112/140‑1 (MQ=255)
                                                  aTGCGGCGTAAACGCCTTAGCCTGCCTGCAAATGCGAAGTTCAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTcaa                                                                                        <  2:1002549/143‑1 (MQ=255)
                                                      ggcgTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCCGCCACGATGCTGCTGAATGGCGGTTTCAACAAt                                                                                    >  2:598113/1‑143 (MQ=255)
                                                       gcgTAAACGCCTTATCCTGCCTGCAACTGCGAAGTTTAACTCCGCTTATACAGCGGCCACCAAATCACAAGCCGTAAACCGCCCCGCGGCGGTCTGCTGCCATCACCCAGCAGCGATGCTGCTGACTGCCGGTGTCAACAATc                                                                                   >  1:441961/1‑143 (MQ=255)
                                                       gcgTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATc                                                                                   <  2:306943/143‑1 (MQ=255)
                                                       gcgTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATc                                                                                   <  1:96820/143‑1 (MQ=255)
                                                         gtcagtggctcagccttcctgCAATTGCGAAGTTTAACTTCGCGTATACAGCGTCAATCACATCACCAGCTGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTTAATGGCGGTTTCAACAATCGc                                                                                 <  1:285974/130‑1 (MQ=255)
                                                                    acgctgcctgcAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAAc                                                                      <  1:797310/140‑1 (MQ=255)
                                                                    aTCCTGCCTGCAAATTCGACGTTGAACTCCGCTTACACAGCGGCAACCAAATCGCCAGCCGTAAACCGCCCCGCGGCTGTCTTCTGCCTTCACCCCGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAAc                                                                      <  1:805573/143‑1 (MQ=255)
                                                                     tCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAGcc                                                                     >  2:641141/1‑143 (MQ=255)
                                                                               aaaTGCGAAGGTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCa                                                           <  2:774020/143‑1 (MQ=255)
                                                                                           tAACTCCGCTTATACAGCGGCAACCAAATCACCACCCGTACACCGCCCAGCGCCCCTCTTCTGCCTTCACCCACCCACGATGCTGCTGAATGGCGGTCTTACCCATCGCCACCCCCAACCCTCTCACGCCAGACCCACCATcg                                               >  1:392879/1‑143 (MQ=255)
                                                                                               tCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATcgcggt                                           >  2:368023/1‑141 (MQ=255)
                                                                                               tCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACCACcgcgca                                           >  1:562834/1‑141 (MQ=255)
                                                                                                 cGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCt                                         <  2:285574/143‑1 (MQ=255)
                                                                                                 cGCTTAGACAGCGGCGCGCGAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTGCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCt                                         <  2:843374/143‑1 (MQ=255)
                                                                                                          aGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTa                                >  2:817542/1‑143 (MQ=255)
                                                                                                          aGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCCGAATGGCGGTTTCAACAATCCCCAGCCCCACACCAGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTa                                <  2:220300/143‑1 (MQ=255)
                                                                                                             ggCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGa                             <  2:277390/143‑1 (MQ=255)
                                                                                                              gCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTAGCGCCAGCTTCACGATCGCGTGCTCCATCGGCCCGAt                            >  1:508482/1‑143 (MQ=255)
                                                                                                               caaagacctcgccAGCCGCAAACCGCCCAGCGGCTGCCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATa                           <  1:474857/132‑1 (MQ=255)
                                                                                                                aaCCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCCGTCTCAACAATCGCCAGCCCCAAAGCTGGACCGCCAGACTCACGATCGCGTCCTCCCTCGGCACGCTac                          >  1:159438/1‑142 (MQ=255)
                                                                                                                  ccAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGCTGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCCAACCTGTACCGCCAGATTCACGATCGCGTGCTCCATCgggcccctcgta                        >  1:371403/1‑133 (MQ=255)
                                                                                                                   gaaatgcgcAGCGGTGAACCGCCTAGCGGCGGTCTTCTGCCTGCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTTTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGagc                       <  1:62083/135‑3 (MQ=255)
                                                                                                                    aaaTCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAacg                      <  2:1035624/143‑1 (MQ=255)
                                                                                                                     aaTCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAacgg                     >  2:985418/1‑143 (MQ=255)
                                                                                                                     aaTCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATACTGCTCGATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGACAGATTCACGATCGCACGCTTCCTCGGTACGATAGAacgc                     >  1:130684/1‑142 (MQ=255)
                                                                                                                          ccAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCAGAATGGCGGCATCAACAATCGCCAGCCCCCAACCTGTACCGCCAGAGTAACGATCGCGTGCTTCATCGGTACGAtgaaacagaacga                >  2:593980/1‑131 (MQ=255)
                                                                                                                               cGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATc           <  2:1040193/143‑1 (MQ=255)
                                                                                                                                  aaaCCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGt        <  1:631227/143‑1 (MQ=255)
                                                                                                                                    aCCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGCGGGAGAATCGGtgc      >  2:675791/1‑141 (MQ=255)
                                                                                                                                     ccGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTcg     >  2:1005995/1‑143 (MQ=255)
                                                                                                                                      cGCCCAGCGGCTGTCCTCTGCCCCCACCCAGCCACGATGCTGCTGAATGGCTGTTTCACCGACCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTcgc    <  2:405697/143‑1 (MQ=255)
                                                                                                                                        cccAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGa  >  2:644420/1‑143 (MQ=255)
                                                                                                                                        cccAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGa  <  2:439668/143‑1 (MQ=255)
                                                                                                                                        cccAGCGGCTGGCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTGCCACAATCGCCAGCCCCAATCCTGTACCGCCAGCTTAACGATCGTGATTGTCATCGGTTCGATAGCACGGGCGGAGCATCTGTGcgcgg  >  2:40687/1‑142 (MQ=255)
                                                                                                                                              |                                                                                                                                         
CCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGA  >  NC_000913/4103511‑4103790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: