Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1299499 1300697–1299500 2–1199 95 [0] [2] 98 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)90 (1.180) 70/276 0.0 98.9% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 2 (0.030)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  >  NC_000913/1299365‑1299640
                                                                                                                                     |                                                                                                                                              
ttagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatc                                                                                                                                       >  1:1246500‑M1/1‑134 (MQ=255)
  agttaTAAGTATTTCCTTAACTGCTTCTCCTCAACATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaa                                                                                                                                     <  2:611540‑M1/143‑12 (MQ=255)
  agttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaa                                                                                                                                     <  1:1105869‑M1/143‑12 (MQ=255)
    ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                   <  1:1126515‑M1/143‑14 (MQ=255)
    ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGCTAGAGGAACGTGCTAAataataatcaatt                                                                                                                                   >  2:697487‑M1/1‑130 (MQ=255)
      ataAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAACAATAATTGAAATGATATTATTAATTCCACTGCCTTTCGTAGAGCAAAGTGCTAActaataatcaattgt                                                                                                                                 >  2:485186‑M1/1‑128 (MQ=255)
       taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                                >  1:669675‑M1/1‑127 (MQ=255)
        aaGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtta                                                                                                                               <  2:985035‑M1/143‑18 (MQ=255)
         aGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATTATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                                                                                                              <  1:1128337‑M1/143‑19 (MQ=255)
         aGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                                                                                                              >  1:266771‑M1/1‑125 (MQ=255)
          ggATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTCATTCCACTGCCTTTGGTAGCGGAAAGTCCTAAatactaatcaattgttaag                                                                                                                             >  2:522530‑M1/1‑124 (MQ=255)
           gATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                                                                                                                            <  1:321388‑M1/143‑21 (MQ=255)
            aTTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatt                                                                                                                           >  1:1294572‑M1/1‑122 (MQ=255)
                    aaCTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgca                                                                                                                   >  2:705215‑M1/1‑114 (MQ=255)
                      cTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatt                                                                                                                 >  1:1251979‑M1/1‑112 (MQ=255)
                          ttCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGATAAataataatcaattgttaaattattgagcatttcac                                                                                                             >  2:766118‑M1/1‑108 (MQ=255)
                             tccttACCATCATGTTATTTTCTCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactac                                                                                                          <  2:195706‑M1/143‑39 (MQ=255)
                             tcctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGGAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatagtgcatttcaacac                                                                                                          >  2:249779‑M1/1‑105 (MQ=255)
                             tcctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTACTAAataataatcaatagttaaattattgtgcatttaactaa                                                                                                          >  2:950340‑M1/1‑105 (MQ=255)
                                tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                                                                       >  1:1222940‑M1/1‑102 (MQ=255)
                                 cACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAACGTGCTAAataataatcaattgttaaattattgtgcattgcactactgga                                                                                                      >  1:181402‑M1/1‑101 (MQ=255)
                                  gccaTCATGTTCTTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataataaattgttttattattgtgcatttcactactggaa                                                                                                     <  2:168578‑M1/142‑44 (MQ=255)
                                  aCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAatcataatcaattgtgaaattattgtgcatttcactactggaa                                                                                                     <  1:936755‑M1/143‑44 (MQ=255)
                                  aCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                     >  2:1054985‑M1/1‑100 (MQ=255)
                                  aCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                     <  1:451774‑M1/143‑44 (MQ=255)
                                  aCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                     >  1:725975‑M1/1‑100 (MQ=255)
                                  aCCATAATGTTATTGTCGGCAAAACATATTGCTGTGCTTGTTGAAGAATAATGGAAATGATATTATTAATTCCACTGGCGTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                     <  2:148993‑M1/143‑44 (MQ=255)
                                  aCCAGCAGGTTTTTTTCGGGACTTCATAATCCTGGGCTTGCTGAAGCATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                     <  2:565371‑M1/143‑44 (MQ=255)
                                     atcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactg                                                                                                  <  1:287909‑M1/143‑47 (MQ=255)
                                         tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                              <  2:885562‑M1/143‑51 (MQ=255)
                                         tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                              >  1:268536‑M1/1‑93 (MQ=255)
                                         tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCATTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcatttctggaactgtaat                                                                                              >  2:876879‑M1/1‑93 (MQ=255)
                                         tGTTATTTTAGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                              <  2:691480‑M1/143‑51 (MQ=255)
                                          gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTGGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatc                                                                                             <  2:822343‑M1/143‑52 (MQ=255)
                                           ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                            >  1:898874‑M1/1‑91 (MQ=255)
                                           ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                            >  1:1187881‑M1/1‑91 (MQ=255)
                                               tttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaa                                                                                        <  1:198935‑M1/143‑57 (MQ=255)
                                                ttCCCCACATCATAATCCTGGGCGTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                       <  2:689140‑M1/143‑58 (MQ=255)
                                                  cGCCACATCATAATCCTGCGCTTGCTGAAGAATAATTGAAATTATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaaga                                                                                     <  1:590229‑M1/143‑60 (MQ=255)
                                                   gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagat                                                                                    <  1:783511‑M1/143‑61 (MQ=255)
                                                    ccgcgtcagaaggctgggctggccgaaGCAAACTTGCAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatggtgcatttcactactggaactgtaatcagaaaagata                                                                                   <  2:1020876‑M1/130‑62 (MQ=255)
                                                    acacatCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                   <  1:908506‑M1/142‑62 (MQ=255)
                                                      acaGCATAAGCCTGGGCTTGCGGAAGAATAATTGAAATGATATTATTAATTCCAGTGCGTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                                 <  2:474732‑M1/143‑64 (MQ=255)
                                                         tcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacat                                                                              <  1:1257100‑M1/143‑67 (MQ=255)
                                                           atAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGTCTTTGGTAGAGGATAGTGCTAAataataatcaattgttagattgttgtgcatttcactactggaactgtaatcagaaaagatagacatgc                                                                            <  2:902675‑M1/143‑69 (MQ=255)
                                                           atAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaactgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgc                                                                            >  1:186867‑M1/1‑75 (MQ=255)
                                                                   gggCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaa                                                                    >  1:517061‑M1/1‑67 (MQ=255)
                                                                           tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctttatt                                                            >  1:824870‑M1/1‑59 (MQ=255)
                                                                             aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttg                                                          >  1:615567‑M1/1‑57 (MQ=255)
                                                                               gaaTAATTGAAATGATATTATTAATTCCACTTCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgat                                                        <  1:208392‑M1/143‑89 (MQ=255)
                                                                               gaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcttcttgtaatgggatctgtaatcagaaaagatagacatgcttagccaatctctatttgat                                                        >  1:626190‑M1/1‑55 (MQ=255)
                                                                               gaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgat                                                        <  1:702068‑M1/143‑89 (MQ=255)
                                                                                aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAgtaataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                       <  1:1111755‑M1/143‑90 (MQ=255)
                                                                                   aatTTAAATGATATTATTAAGTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                    <  1:370520‑M1/143‑93 (MQ=255)
                                                                                   aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAGataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagagatgcttagccaatctctatttgattgag                                                    >  2:399530‑M1/1‑51 (MQ=255)
                                                                                   aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                    <  2:683813‑M1/143‑93 (MQ=255)
                                                                                   aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                    >  2:779921‑M1/1‑51 (MQ=255)
                                                                                   aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                    <  1:241066‑M1/143‑93 (MQ=255)
                                                                                     ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatt                                                  >  2:216220‑M1/1‑49 (MQ=255)
                                                                                     atggaatgttATTATTAATTCCACTGCCTTTGGTAGAAGGAAGTGCTAAataataatcaattgttaaattattgtgcatttcactaccggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatt                                                  <  2:54116‑M1/139‑95 (MQ=255)
                                                                                         aaTGATATTATTAATTCCACTGCTTTTGGTAGAGGAAAGTGCTAAataataatcaatttttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                              <  2:301035‑M1/143‑99 (MQ=255)
                                                                                              tattatTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagacactctatatattattaaattgaaattttt                                         >  1:1344295‑M1/1‑40 (MQ=255)
                                                                                                ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgaatgaactgaaaaatgttt                                       >  2:1364946‑M1/1‑38 (MQ=255)
                                                                                                  attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatcgccacgctaggcgaacggctatgggattgacctgaaagatgtttgt                                     >  2:1321057‑M1/1‑36 (MQ=255)
                                                                                                  attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgt                                     >  1:1336594‑M1/1‑36 (MQ=255)
                                                                                                  attaATTCCACTGCCTTTGGTAGAGGAAAGTGCGAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgt                                     <  1:558192‑M1/143‑108 (MQ=255)
                                                                                                   ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtt                                    >  1:750904‑M1/1‑35 (MQ=255)
                                                                                                   ttaAGTGCAGTGTCTTTGGTAGAGGAACGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagtattgatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtt                                    <  1:446397‑M1/143‑109 (MQ=255)
                                                                                                     aaTTCCACTGCCTTTGGAAGAGGAAAGGGCTAAataataatcaattgttaaattatcgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaa                                  <  1:422990‑M1/143‑111 (MQ=255)
                                                                                                      aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                 <  1:784037‑M1/143‑112 (MQ=255)
                                                                                                      aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                 >  2:727954‑M1/1‑32 (MQ=255)
                                                                                                         ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggca                              >  1:204058‑M1/1‑29 (MQ=255)
                                                                                                          cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcat                             >  1:172133‑M1/1‑28 (MQ=255)
                                                                                                           aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgcttgttaaggcatg                            >  2:510196‑M1/1‑27 (MQ=255)
                                                                                                              gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                         <  1:1264217‑M1/143‑120 (MQ=255)
                                                                                                              gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                         >  1:202578‑M1/1‑24 (MQ=255)
                                                                                                                    ggTAGAGGAAAGTGTGAGataataatcaattgttaaagtattgtacatttcactagtggaactgtaatcagaaaagatagatatgcttagccaagctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagc                   <  2:510555‑M1/143‑126 (MQ=255)
                                                                                                                     gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgggactgtactcagaaaggatagacatgcttagccactctctatgtgattggattggaagatgtttgttcaggcgtgggtggaggct                  >  1:363932‑M1/1‑17 (MQ=255)
                                                                                                                     gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagct                  >  1:1349205‑M1/1‑17 (MQ=255)
                                                                                                                      tAGAGGCAAATGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagacaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgccagcga                 >  2:194435‑M1/1‑16 (MQ=255)
                                                                                                                       agagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                <  2:114572‑M1/143‑129 (MQ=255)
                                                                                                                        gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctata               <  2:903805‑M1/143‑130 (MQ=255)
                                                                                                                         agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagcaaagatagacatgcttagccaatctctatttgattgaattgaacgatgtttgttaaggcatggatgcaagctacag              >  2:1045998‑M1/1‑13 (MQ=255)
                                                                                                                         agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagaggtttgttaaggcatggatgcaagctatag              >  2:362854‑M1/1‑13 (MQ=255)
                                                                                                                             aaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc          <  1:705215‑M1/143‑135 (MQ=255)
                                                                                                                             aaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc          <  1:363163‑M1/143‑135 (MQ=255)
                                                                                                                             aaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc          <  1:714527‑M1/143‑135 (MQ=255)
                                                                                                                              aGTGCTTAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattct         <  2:1354924‑M1/143‑136 (MQ=255)
                                                                                                                               gTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctg        <  1:981771‑M1/143‑137 (MQ=255)
                                                                                                                                tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctga       <  2:266771‑M1/143‑138 (MQ=255)
                                                                                                                                   tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatac    >  1:217017‑M1/1‑3 (MQ=255)
                                                                                                                                   tAAataataatcaattgttaaattattgtgcatttcactactgaaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagctgtttgttaaggcatggatgcaagctatagattctgatac    >  2:73927‑M1/1‑3 (MQ=255)
                                                                                                                                    aaataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacg   >  2:518219‑M1/1‑2 (MQ=255)
                                                                                                                                    aaataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcaaggatgcaagctatagattctgatacg   <  2:486206‑M1/143‑142 (MQ=255)
                                                                                                                                     aataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagcccatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagaaatagattctgatccga  >  2:1084559‑M1/1‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                              
TTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  >  NC_000913/1299365‑1299640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: