| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,267,595 | A→G | *73* (TAA→TGA) | tdcR → | L‑threonine dehydratase operon activator protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,267,595 | 0 | A | G | 90.0% | 145.4 / 7.1 | 62 | *73* (TAA→TGA) | tdcR | L‑threonine dehydratase operon activator protein |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base G (35/19); total (38/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.35e-01 | |||||||||||
CTATTTGTTGGAGAACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAG > NC_000913/3267455‑3267734 | ctttttGTTGCACAACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATgaa < 1:411759/140‑1 (MQ=255) tATTTGTTGGAGAACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCCTAAAt > 2:953700/1‑143 (MQ=255) ttgGAGAACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATtcttct > 1:100538/1‑143 (MQ=255) ggAGAACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTcc > 2:717265/1‑143 (MQ=255) gagaACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTccc > 2:723002/1‑143 (MQ=255) agaACAACAATCTATTTTATCAATATTTTAAAATCTCGCTTCTACGCATGGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTcccc < 2:770225/143‑1 (MQ=255) gaACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCt < 1:264565/143‑1 (MQ=255) acaaacaTTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCa < 1:71683/143‑1 (MQ=255) caaacaTTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCAt < 2:547337/143‑1 (MQ=255) atttatttTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTATCTTCTGCCAGGACGTCAGTTTGTGAGTGCCACTGTTTTCCATGAATTGTTCTCGCCTCATAGGcg > 1:589836/1‑143 (MQ=255) tttattttatCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGcgc > 2:700966/1‑142 (MQ=255) tttattttatCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGcga > 1:662061/1‑143 (MQ=255) tttattttatCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGcga < 1:947166/143‑1 (MQ=255) ttttatCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGaa < 2:919958/143‑1 (MQ=255) tcttatCAATATTTTAAAATTTCGAATACATGTATTGATCAGCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGaa < 2:408947/141‑1 (MQ=255) ttatGAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATa < 1:303905/143‑1 (MQ=255) ttatCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTCCCATGAATTCTTCTCCCCTCATAGGCGACGAATa < 1:665789/143‑1 (MQ=255) tatTGTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTGCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCAtttt < 2:259565/143‑1 (MQ=255) tttAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTtgtg < 2:859071/143‑1 (MQ=255) tttAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTtgtg > 1:53518/1‑143 (MQ=255) tttAAAATTTCGAATACATGTATTGATCATCGCGAACAATTGATTAACGTCAACTTTTTCGCTTCTGACAGGACGTCATTTTGTGAATGAAATCGTTTTCCATGGATTATTATCTCATCATTGTCGACGGATATCATTTggtg > 1:562963/1‑143 (MQ=255) tAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGtt > 2:891802/1‑143 (MQ=255) tttCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGa > 1:1009698/1‑143 (MQ=255) ttCTAATATATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGCATGCCATTGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGAt < 2:99418/143‑1 (MQ=255) aaTACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGTCGAATAGCATTTTTTGTGGAgcctccca > 1:744527/1‑136 (MQ=255) aCATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACaaaa < 2:937220/143‑1 (MQ=255) aTGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTATGTTGAGGATCAAAAAAca > 1:80716/1‑142 (MQ=255) gtgtcgttcatcTCCACCACTTGATGAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGaa < 1:518189/136‑1 (MQ=255) tATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGGATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATCGGCGACGAAGAGCATTTTGGGTTGGGGATGACAGGACGaag > 2:256338/1‑142 (MQ=255) tATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGaat > 2:42382/1‑143 (MQ=255) gATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAAtt < 1:717265/143‑1 (MQ=255) atcatcTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTGGAGGATCCCAAAACAAATAATTg > 1:850688/1‑143 (MQ=255) tcatcTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGCCAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCGTCATAGGCGACGAATAGCATTTTGTGTTGAGGATGACAGACCGAATAATTgt > 2:271697/1‑142 (MQ=255) tctcGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGa > 2:747348/1‑143 (MQ=255) tctcGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGa > 1:603486/1‑143 (MQ=255) gAACAATTGATTAACGTCAACTTTTTCTCTTCAGACAGGACGTCATATTGTGAATGAAATCGTTTTCGATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGATGAGGATCACAAAAAGAATAATTGCTGAtacg > 1:534786/1‑140 (MQ=255) taCAATTGATTAACTTCAACTTTTTCTTTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATcgcc < 1:382665/142‑1 (MQ=255) aaCAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATcgcc > 2:1005377/1‑143 (MQ=255) aaTTGATTAACGTCAACTTTTTCTCTTCTGACATGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCcgcg > 1:646664/1‑143 (MQ=255) aTTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTGGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAg < 1:1009185/143‑1 (MQ=255) aaCGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTc < 2:603486/143‑1 (MQ=255) accttttCTCTTCTGAGAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaa < 1:544959/140‑1 (MQ=255) tttttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagaccaaga > 2:130033/1‑143 (MQ=255) tctcTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGacaac > 2:745176/1‑143 (MQ=255) cttctGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAg > 1:171404/1‑143 (MQ=255) aaattttattaaCGTCATTTTGTGGATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAACAGCATTTTGTGTTGTGGGTCACCAAACGAATAATAGCTGATCGACGACATCAGGTAAGACAAAGAGAACAAg > 1:605542/12‑143 (MQ=255) ctGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGa > 2:839231/1‑143 (MQ=255) ctGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGa > 2:505168/1‑143 (MQ=255) gACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGaaa > 2:593719/1‑143 (MQ=255) gACGTCATTTTGTGAATGCAATCGTTTTCCATGACTTCTTCTCCCCTCATAGGCGACGAATAGCATTTGGTGTTGAGGATCACAAAAAGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTc < 2:329724/143‑1 (MQ=255) gACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATGTTc > 1:125134/1‑143 (MQ=255) ttGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCGCAGAGCtt > 2:171778/1‑143 (MQ=255) ttGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATGGCTGATCGCCGCGATAGGGTCAGACGAAGACAACAAGGGAAACTTTCGCGGAGCtt > 2:92542/1‑143 (MQ=255) tgtgAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCttt > 2:798383/1‑143 (MQ=255) gAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAAAGAATAATTGCTGATCGCCGCGATAAGGTCACACGATGACAACATGGAACACTTTCACACAGCTTTTGa > 1:901385/1‑143 (MQ=255) aaaTGGAATCGTTTTCCATTAATTCTTCTCCCCGCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGa < 1:1022772/142‑1 (MQ=255) tGCAATCGTTTTCCATTAATTCTTCTCCTCTGATAGGCGACGTGTCAATATTTATAGTGAAAACAACTATACGTATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCg < 1:470766/143‑1 (MQ=255) aatagtaatCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCg > 1:990650/9‑143 (MQ=255) aaTCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCg > 2:459594/1‑143 (MQ=255) aTCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAACGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGt > 2:268953/1‑143 (MQ=255) tCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTa < 2:80716/143‑1 (MQ=255) tttCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGcc > 1:100471/1‑143 (MQ=255) cATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAg < 1:240976/143‑1 (MQ=255) | CTATTTGTTGGAGAACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAG > NC_000913/3267455‑3267734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |