| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,977,578 | T→G | S328A (TCA→GCA) | galR → | galactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,977,578 | 0 | T | G | 95.6% | 122.2 / ‑5.2 | 47 | S328A (TCA→GCA) | galR | galactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (26/17); total (28/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTCCGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAA > NC_000913/2977439‑2977714 | gCGTCCGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAg < 2:5388/143‑1 (MQ=255) tCCGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGCAATCACTAATGGCTTTAGTGCGACGCTGGTACGTCGTCATGCAgtgt > 2:217/1‑143 (MQ=255) gcCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACt < 2:857929/143‑1 (MQ=255) accGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCt > 2:480042/1‑143 (MQ=255) gTGCGTTACCCAAGCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGa < 2:353034/143‑1 (MQ=255) tgctgttcccAATCGTGACTATGCCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAg < 2:18607/136‑1 (MQ=255) tGCGTTACCCAATCGTGACGCTGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAg < 1:1032386/143‑1 (MQ=255) tGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGACATCACTAATGTCTTTAGTCCGACGCTGGTCCGTCGTCAGGCAGTGTCAACTCCGTccgtgggg > 1:865431/1‑136 (MQ=255) gCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAgg > 2:500834/1‑143 (MQ=255) gTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTAGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGGCTTTAGTCCGACGCTGGTAAGTCGTCACGCACTGTCAACTCAGTCGAGGGACGCa > 1:383774/1‑143 (MQ=255) cccAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAGGtc > 2:87528/1‑143 (MQ=255) ccAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACGCCGTCGCTAGaggcaaggca > 1:248879/1‑143 (MQ=255) ccAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTATCTAGTCAGACGCCGGTACGTCGCCACGCCGGGTCACCTCCGCCGCTGGAGGCAAGtca > 1:700474/1‑143 (MQ=255) tCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTcatcat < 2:122838/143‑1 (MQ=255) tGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCAGGAAATCAATAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTTGAGGCAAGTCATCATGcc > 2:131670/1‑142 (MQ=255) aCGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGGCGCTGGAAAGCCGTCACTCTCTGTCAACGTCGTCGCTGGAGGCAAGTCATCATGCAAc < 2:450524/143‑1 (MQ=255) tGGCGACCCAGGCTGTCGAACTGGCTTTGGCGCTGTCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGc < 1:670138/143‑1 (MQ=255) gCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGa < 2:46042/143‑1 (MQ=255) cGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGAc < 2:842202/143‑1 (MQ=255) cGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGGACGTCGTCATGCAGTGTCAACTCCCTCCCTCGACGGACGCCCCCATGCAACCCGCGAc > 1:780245/1‑143 (MQ=255) gACCCAGGCTGCCGAACTGGCTTTGGAGCTGGCGGATAATCGCCCTCTCCCGGAACTCACTAATGTCTTTAGTACGACGCTGGTCCGTACTCATTCAGTGTCAACTCCGCCTCTGTAGGCAATCCATCATGCAACCAgagtcg > 1:585736/1‑138 (MQ=255) tGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGt > 2:1061374/1‑143 (MQ=255) tGGCTTTGGCGCTGGCGGATACTCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCACCTCCCTCGCCGCAGGCAAGTCATCATGCAACCAGCGACCAACCGCAGTTCAAGcca > 1:308102/1‑141 (MQ=255) tGGCTATGGCGCGGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTATTCCGCCGCTGGTACGTCGTCATACAGTGTCAACGCCGTCGCTGGAGGAAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCaa < 2:128959/143‑1 (MQ=255) tttGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGGCAGGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACAAACCTCAGTTACAGCacgtcc > 1:789926/1‑138 (MQ=255) ttgggCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTcc < 1:387630/140‑1 (MQ=255) cgcTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAACCAAGTCTAgcgc > 2:389316/1‑143 (MQ=255) cTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCa < 2:4364/143‑1 (MQ=255) tGGCGGTGAAGCGCGCGCGCCCGGGAGTCACGAATGGCTTTAGTCCGACGCGGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAg < 1:41671/143‑1 (MQ=255) tGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAg > 2:1036553/1‑143 (MQ=255) gCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCCTGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGtc > 1:902850/1‑142 (MQ=255) aaTCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAttctt > 2:706908/1‑143 (MQ=255) cGCCCTCTCCCGGAAATCACTAATGTCTTTACTCCGACCCTGGTACGTCAACATCCAGTGTCAACTCCTTCGCCGCAGGCAAGTCATCATGCAACCCGCGACTAACCGCAGTTACAGTAATCCCATCTCCATGAAttctccca > 1:112474/1‑139 (MQ=255) ctctCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGt > 2:267742/1‑143 (MQ=255) ccGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCACGTCATCATGCCACCAGCGACTAACCGCAGTTAAAGCAATTCCCGCGCCAGTAATTCTTCGGTGGCCTGGc > 2:424140/1‑143 (MQ=255) gAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGAc < 1:500834/143‑1 (MQ=255) gAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGAc < 1:601544/143‑1 (MQ=255) ttCACTGATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGTAAGCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGc < 1:516574/142‑1 (MQ=255) aCTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTCCGATGGTCTGGCGACGGCGaa > 2:66605/1‑143 (MQ=255) gTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGCCTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTg > 2:126966/1‑143 (MQ=255) tCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGa > 2:414977/1‑143 (MQ=255) cGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCCTCATGCAACCAGCGACTCACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGAcc > 1:857487/1‑143 (MQ=255) gatgCTGGCACGTCGTCATGGAGGGTCAACCGCGTCGCTGGAGGCAAGTCAGCATGCAACCAGCGACTAACCGCAGTGAAAGCAATCCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCa < 2:284018/140‑1 (MQ=255) gACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCCGCGACTAACCGCAGTTAAAGCACTTCCAGCGCCAGTCATTCTTCGATGGTCTAGCGACGGCGAATCAACCGCGCCTAACCa > 1:814744/1‑143 (MQ=255) gACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTgcaca > 2:407312/1‑139 (MQ=255) gACGCTGGTACGTCGTCATGCAGTGTCAACTCCGACGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCa > 2:639221/1‑143 (MQ=255) cgtcATGCGGTGTCTCCTCCGTCGCGGGAGGCAAGTCAGCATGCAACCAGCGACTAACCGCCGGTAAAGCAATCCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGaa < 1:1007722/143‑1 (MQ=255) | GCGTCCGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAA > NC_000913/2977439‑2977714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |