Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	4,001,644	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001643	1 (0.020)	44 (0.730)	42/282	0.4	97.8%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001649 =	1 (0.020)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

GATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4001508‑4001643 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCA  >  NC_000913/4001649‑4001789                                                                                                                                                                                                                                                                                       GATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTC                                                                                                                                        <  2:404700/143‑1      ATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTT                                                                                                                                   >  2:183478/1‑143       TCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGCAGACGACGACGGCATGCATCGCCTGCTTCTgc                                                                                                                                  >  2:62443/1‑141         TTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTT                                                                                                                                <  2:300738/143‑1            TCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGA                                                                                                                             <  1:783752/143‑1                 CCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCCCCCGGAACTGGAAGACATCGAAGCCTCGGCACGTTTCTTTGACGACGACGACGGCCTGCATCTTCCACCACTTCATTGACGATG                                                                                                                        >  2:30162/1‑143                  CGGACGACGACGAGCGACTGCGCCTACAATCGGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGC                                                                                                                       <  2:717772/143‑1                    GACGACGGCGAGCGACTGCGCGTTCTCTCTGAACGTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGG                                                                                                                     <  1:690201/143‑1                      CGACGACGAGGGACTGCGCGTACAATCGGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTTAAGATGCGGAA                                                                                                                   <  2:694095/143‑1                       GACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAG                                                                                                                  >  2:800652/1‑143                               GCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGACCACGCC                                                                                                          >  1:781538/1‑143                                 GACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGG                                                                                                        <  2:409215/143‑1                                      CGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACT                                                                                                   >  2:169881/1‑143                                             AATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAAATGGAAGACGTCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCGTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGT                                                                                            <  2:393393/143‑1                                                  GAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCAT                                                                                       >  2:169810/1‑143                                                         GCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCAT                                                                                <  2:438817/143‑1                                                          CCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGCTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGCGGAATcacgcatc                                                                               >  1:909784/1‑135                                                           CAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGCTAACTCCACTGTGGCATTTACCATaa                                                                              >  2:652329/1‑141                                                               GCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGA                                                                          <  2:674041/143‑1                                                                CCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT                                                                         <  2:927673/143‑1                                                                    GCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTC                                                                     <  1:891058/143‑1                                                                      agCCCGCCCGTGACTGGAAGACTTCGAAGCATCGGCACGTTTCTTTGAAGCCGACGACGCCCTTCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGT                                                                   <  1:921136/141‑1                                                                          CGCCCGTATCTGGAAGACATCGAAGCATCTGCACTTTTCTTTGAAGTCGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCTGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGT                                                               <  1:569047/143‑1                                                                              CGGAGCTGGAAGACATCGAAGCATCGGCGCGTTGCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCGTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAC                                                           <  2:2915/143‑1                                                                                   CTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGC                                                      >  2:333372/1‑143                                                                                            ATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTG                                             <  1:633990/143‑1                                                                                            ATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCATCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTG                                             >  2:51869/1‑143                                                                                             TCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCAGCCGTGTGGGTCGTCTGTTTACTCTGCGTGAGCGTGA                                            <  2:730327/143‑1                                                                                              CGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACAATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAA                                           >  2:410395/1‑143                                                                                               GAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTACCTCCCCTGTGGCATTTACCATCCTTGATGCACAAATGTAAATTCTGAGTGAGAGTGAAC                                          >  2:675329/1‑143                                                                                                gAGCATCGGCACGTGTCTTTGATGCCGACGACGGCCTGCATCTCCTTGATCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCTCTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACT                                         <  1:762427/142‑1                                                                                                AAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAATCTGCGTGAGCGTGAACT                                         >  2:632914/1‑143                                                                                                     tctGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCG                                    <  1:284690/140‑1                                                                                                             TTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTAGCATCCGTGATGGTCGTCTGTTTACTCTGAGTGAGCGTGAACTGCGCGCTTTTGGT                            >  2:123747/1‑143                                                                                                             TTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCCGTTTACTCTGCGTGCGCGGGAACTGCCCGCCTTTCGT                            >  2:376291/1‑143                                                                                                              TTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAGCTGCCCGCCTTTCGTC                           >  1:484586/1‑143                                                                                                                ctcTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGTTGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTG                         <  1:1002478/140‑1                                                                                                                    GAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGAATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATC                     <  2:398977/143‑1                                                                                                                     aacACGACGCCGGCCTGCATCTCCCTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCG                    <  2:575550/140‑1                                                                                                                       GACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCCTGAACTGCCCGCTTTTCGCCTGTATCGTA                  >  2:929063/1‑143                                                                                                                                   CTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCCCTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAAGCGTGCCCGTA      >  1:415022/1‑143                                                                                                                                   CTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT                          <  2:405525/123‑1                                                                                                                                   CTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT                          >  1:405525/1‑123                                                                                                                                       ATCTCCTTCTTCTGCTTTGAAGAGGCGGAAGATCACGCCGGTTACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGTCA  <  1:812474/143‑1                                                                                                                                                                                                                                                                                       GATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4001508‑4001643 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCA  >  NC_000913/4001649‑4001789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.