| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 95.0% | 109.1 / NA | 41 | I204S (ATC→AGC) | wcaA | putative glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (18/20); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAC > NC_000913/2132647‑2132920 | ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTc > 1:546235/1‑143 (MQ=255) gtcgggTAGAGGTTGAGCAGCTGAGAGTTTTGGCTGGCGGGGTGGAATTTGGCTTTGTGCTTGCTGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTg < 1:387852/139‑1 (MQ=255) aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTg > 2:541473/1‑143 (MQ=255) gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg > 1:467003/1‑143 (MQ=255) tggacggTAAGCTGCTGATATTTGTTGCTTGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGATGTATCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 1:61410/138‑1 (MQ=255) tAGAGGGTAAACAGCTGATCTTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 2:487426/143‑1 (MQ=255) agGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctc < 2:281337/143‑1 (MQ=255) gTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc < 2:546736/143‑1 (MQ=255) aGCTGATATGTTTTGCTGGCGCGGTCGAATTCGTCTTTGTGCCGGCGGTAAAAGTGGAAGTACCCGGAGAATTTGTTCGGTGACGAGGATATCTCCATTTCACCGTGATTTATATGCAGGCTCTTCGTCGCCTCTTCTACttt < 2:205097/143‑1 (MQ=255) tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa > 2:188900/1‑143 (MQ=255) tGATATTTTTTGCTGGCGCGGTCGAAGTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTAGCCGGAGAATTTTTTCGGTGACAAGGTGATCTGAAGTTCACCGTGAGTGATATGCAGGCTCTGCGTAGCCTCTTCTACttgaca > 1:540546/1‑139 (MQ=255) tatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACgg < 2:661535/143‑1 (MQ=255) gttGCTGTCGCGGTCTAATTTGTCTTTGTGCTTGCGCTCAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGc < 2:431199/142‑1 (MQ=255) cTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAAGTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGGGGGATCTGCGTCGCCTCTGCTACTTTCGACGGTACGCCATa > 2:175271/1‑143 (MQ=255) gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCTGTGACGAGGTGATCTGCATTTCACCGTGAGTGATATGCAGGCTCTTCGTCGCCTCTTTGATTTTTCACGGTTCGTCGTACtt > 2:151971/1‑142 (MQ=255) gTCGATTTTGTCTTGGTGCTCGCGGTAAAAGTGGAAGGACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTAATTGATATGCAGGCTCTGCGTCGCCGCTTCTACTTTCCACGGTTCGCCGTACTccacca < 2:657019/143‑1 (MQ=255) gcttGTCTGTGTGCTTTCCGTAAAAGTGGAATTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc < 2:434807/141‑1 (MQ=255) gTCTTTGTGCTAGCGGTAAAAGTGGAAGCACCCGGAGAATTTTTTCGGTGATGAGGTGATCTGCATTTCACCGTGATTGGTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa < 1:957693/143‑1 (MQ=255) ttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGGTATGCAGGCTCTGCGTCGCCTCTCTCACTTTCCGCGGTGTGCCGACCCCCACCCCCATCAGCCGGaa > 1:917938/1‑143 (MQ=255) tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAg < 2:201459/143‑1 (MQ=255) gtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGAGTCGGCCCTTCCACTTTCCACGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGa > 2:947563/1‑143 (MQ=255) gCTTGCGGAAAACGTGGCAGCACCCGGAGACTTTTTCCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCCCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATg < 2:255161/143‑1 (MQ=255) ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTACGCCGTACTCCACCACCATCACCCGGACGATGTc > 1:535542/1‑143 (MQ=255) gTAAAAGTGGAAGTACCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGCCGCCTCTTCTACTTTCCACGGTACGCCGTCCTCCACCACCACCCGCAGCATGATGTCGTagt > 1:535961/1‑141 (MQ=255) gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGCCGTact > 1:881495/1‑141 (MQ=255) tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGCCGATGTCCTAATc > 2:870585/1‑143 (MQ=255) tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGATGTCGTAATCCTGTGc > 2:863076/1‑143 (MQ=255) tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCGGATGTCGTAATCCTGTGc > 2:517123/1‑143 (MQ=255) ccGGAGAATTTTTTCGGTGACGCGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAACGCGTCGTAATCCCGTCCCGCTTTCAg > 1:444135/1‑143 (MQ=255) agaATTTTTTCGGTGACGAGGTGAGCTGCATTTCACCGTGATTGATATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTGCg > 2:499427/1‑143 (MQ=255) tttttGTGTGACGAGGTGATCTGCATGTCACCGTGATGGAGATGCAGGCGCTGCGTCGCCGCGTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc < 2:734002/143‑1 (MQ=255) ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg < 2:395606/143‑1 (MQ=255) gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa < 2:881055/143‑1 (MQ=255) aCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTTGCGCGTCCCCCAGACTCACAAACTATCCCCTTGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAacac < 2:444135/143‑1 (MQ=255) gcTGCATTTCACCGTGAGTGTGATGCAGGCTCTGCGTCGCCTCTTCTCCTCTCCACGGGTCGGCGTACGCCACCACCATCCGCAGCAAGACGTCGTAAGCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa < 1:333828/142‑1 (MQ=255) tCACCGTGATTGATATGCAGGCTCTGCGTCGCCGCTCCTACTTTCCTCGGTTCGCCGTACTCCACCACCATACGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGc < 1:881489/143‑1 (MQ=255) cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTTTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGACATACACAg > 1:518178/1‑143 (MQ=255) cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg > 2:922606/1‑143 (MQ=255) attattgttTTGCAGGGTCGGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTTCTCCACCACCATCCGCAGGTAGATGTCTTACTCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACGCTGTGAAACGCCATGCCCAgg < 1:628406/140‑1 (MQ=255) gtgatAAGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATCCGCAGGCAGATGTCGTAATCCTGTGCCGCTTTCAGTCCGGTGACGAACAAACACTCTTTGAACCGCCACGCCCAGGGaac > 1:70914/2‑142 (MQ=255) atacGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc < 2:724243/143‑1 (MQ=255) | GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAC > NC_000913/2132647‑2132920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |