New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2024155 =3 (0.060)60 (1.110) 49/282 0.1 95.2% coding (188/624 nt) rcsA transcriptional regulator of colanic acid capsular biosynthesis
?NC_000913 2101749 = NA (NA)intergenic (‑5/+146) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator

TCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCC  >  NC_000913/2101607‑2101887
                                                                                                                                              |                                                                                                                                          
ccTTCACAGTAAATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAGATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaag                                                                                                                                            <  1:868208/142‑1 (MQ=255)
               aaGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAg                                                                                                                             <  2:239322/143‑1 (MQ=255)
                      cTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGaa                                                                                                                      >  2:687034/1‑143 (MQ=255)
                        tGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAAt                                                                                                                    >  2:524568/1‑143 (MQ=255)
                        tGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATCTCTCTTTTCTTAAGGGAGGCACGCATCAGCGAGGAAAt                                                                                                                    >  1:585457/1‑143 (MQ=255)
                            cGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATCGATACATTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTTCGAGTAAGCGTGAATAttctt                                                                                                                >  1:110580/1‑143 (MQ=255)
                                  cATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCTGGGAAATTCTTCTCGGc                                                                                                          >  1:510451/1‑143 (MQ=255)
                                   aTTTACACGCACAAAATCTGAAAAACGTACCAGCATAACTGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCt                                                                                                         <  2:204806/143‑1 (MQ=255)
                                   aTTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGGGCGACTAAGCGGGCAAATTCTTCTCGGCt                                                                                                         >  2:336952/1‑143 (MQ=255)
                                      tACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAc                                                                                                      >  2:783725/1‑143 (MQ=255)
                                      tACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAc                                                                                                      <  2:713421/143‑1 (MQ=255)
                                      tACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAc                                                                                                      <  1:840477/143‑1 (MQ=255)
                                        cacGCACAAAATCTGAAAAACGTAACAGGATGGAGGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTc                                                                                                    >  2:606661/1‑143 (MQ=38)
                                         acGCACAAAAGCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTGTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCa                                                                                                   <  2:855011/143‑1 (MQ=255)
                                           gCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTACGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAccgagc                                                                                                 >  1:868287/1‑138 (MQ=255)
                                           gCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTCTAGGAAGTATTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACGCAGt                                                                                                 >  1:33282/1‑143 (MQ=255)
                                            cacaAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGCTACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtc                                                                                                >  2:68504/1‑143 (MQ=255)
                                             acaAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtca                                                                                               >  1:442086/1‑143 (MQ=255)
                                              caAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACACCCGTCTCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcat                                                                                              <  1:109865/143‑1 (MQ=255)
                                               aaaaTCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatt                                                                                             >  1:899888/1‑143 (MQ=255)
                                               aaaaTCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGCTTTATTAAGGAAGGTGCGAATAAGCGGGGAACTTCTTCTCGGCTGACTTAGtcatt                                                                                             >  1:434944/1‑143 (MQ=255)
                                                   tCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATCCTTTCTCTCGGCATTTTTGTTTTATTAACGAAGGTGCGAATAAGCGGGGAAATTCTTCTCCGCTCACTCAGtcatttcat                                                                                         >  2:655993/1‑143 (MQ=37)
                                                   tCTAAAAAACGTACTAGCATAAATGAACCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttcat                                                                                         <  1:219103/143‑1 (MQ=255)
                                                     tGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTcatttcattt                                                                                       >  2:129705/1‑143 (MQ=255)
                                                      gAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCatttcatttc                                                                                      >  2:862217/1‑143 (MQ=255)
                                                      gAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCatttcatttc                                                                                      >  1:672048/1‑143 (MQ=255)
                                                      gAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCatttcatttc                                                                                      <  2:640868/143‑1 (MQ=255)
                                                       aaaaaCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttct                                                                                     >  2:936105/1‑143 (MQ=255)
                                                         aaaCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGTAAGGTGCGAATAAGCGGTGTATTTCTTCTTGGCTGATTCAGTCATTTCGTttcttt                                                                                   >  1:839062/1‑142 (MQ=255)
                                                         aaaCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttcttc                                                                                   <  1:783725/143‑1 (MQ=255)
                                                            cGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATg                                                                                <  2:747412/143‑1 (MQ=255)
                                                                 cAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGa                                                                           <  1:115047/143‑1 (MQ=255)
                                                                             tCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtc                                                               <  1:687034/143‑1 (MQ=255)
                                                                                 gctcACCAATCAACATCCGTATCAGAATAGATACTTTCTTTAGGAATGTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCTTTTCATTTCTTCATGTTTGAGCCGATTTTTTCTccc                                                           <  2:494701/140‑1 (MQ=255)
                                                                                     aCCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAATCGGGGAACTTCTTCTCGGCTGACTCAGTCATTTGATTTCGTCATGTTTTAGCCTCTTCTTTCTGCCTTac                                                       >  1:594295/1‑142 (MQ=21)
                                                                                           caacaaCCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCt                                                 <  1:606661/143‑1 (MQ=255)
                                                                                           caacaaCCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATCTTTTCTCCCGTAACTGCCt                                                 >  2:708022/1‑143 (MQ=255)
                                                                                            aacaacCGTATCAGAATAGATACTTCCTCTAGGAATATTTGCATTATTACCATAGATACGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTACTTCATGTTTGAGCCGATCCTTTCTCCCGCAAATGCCta                                                >  1:290518/1‑142 (MQ=17)
                                                                                               aacCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTCTGAATAAGCGGCGAAATTATTCTCGGCTAATTCATTGTTTTCATTTCGTCATGTTTGTGCCGATTTTTTCTCCCTTAAATGTCTTgtc                                             >  1:573139/1‑141 (MQ=255)
                                                                                               aacCGTATCAGAATAGAAACTTTATTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAATTTCTTCTCGGCTGACTTAGTCATTTCATTTATTCTTGTTTGAGCTGAGTGTTTATTCCGTACATGCTTTGaa                                             >  1:343795/1‑143 (MQ=18)
                                                                                                gccGTATCAGAATAGATACTTTCTTTAGCAATTTTTGTTTTATTAAGGACGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAt                                            <  1:585099/142‑1 (MQ=255)
                                                                                                  cGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTATTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCTTAAATGCCTCGAATCa                                          >  1:588045/1‑143 (MQ=255)
                                                                                                    tATCAGAATAGATCCTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                        <  2:609833/143‑1 (MQ=255)
                                                                                                       cAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGACTCGGCCTa                                     >  2:554601/1‑143 (MQ=255)
                                                                                                        aGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGACTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAGTGCCTTGAAtacgcacat                                    >  1:819395/1‑135 (MQ=255)
                                                                                                        aGAATAGATACTTTCTATAGGAATTTTTGTTTTATTAAGGAAGGTACGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAt                                    >  2:606310/1‑143 (MQ=255)
                                                                                                         gAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGCGCCGATTTTTTCTCCCGTAAATGCCTTGCATCTGCCTAtt                                   >  1:546443/1‑143 (MQ=255)
                                                                                                               aTACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGGGCCGAGTGTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                             <  1:524568/143‑1 (MQ=255)
                                                                                                               aTACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTCGAGCCGATTTTTTCTCCCGTAGCTGCCTTGAATCAGCCTATGTAGAcc                             >  2:296257/1‑143 (MQ=255)
                                                                                                                  ctttctttAGGAATTTTTGTTTTATTACGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGtt                          <  1:516387/143‑1 (MQ=255)
                                                                                                                    ttcattAGGAAGTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttc                        <  1:866936/143‑1 (MQ=255)
                                                                                                                       tttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttcttc                     <  2:877184/143‑1 (MQ=255)
                                                                                                                        ttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCg                    >  1:506207/1‑143 (MQ=255)
                                                                                                                            gAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAt                >  2:824006/1‑143 (MQ=255)
                                                                                                                            gAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGACTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAt                >  2:809332/1‑143 (MQ=255)
                                                                                                                                 tttGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAg           >  2:535445/1‑143 (MQ=38)
                                                                                                                                    gTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTCCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCg        <  2:193171/143‑1 (MQ=25)
                                                                                                                                     ttttATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttctt                                                                                    >  2:540476/1‑66 (MQ=40)
                                                                                                                                     ttttATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttctt                                                                                    <  1:540476/66‑1 (MQ=40)
                                                                                                                                         attaAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCAGAGTTGAGCCCATTTTTTCTCCCGTACATGCCCTTAATCCGCCTACTTAGACCGGTCCTTCGCCACTTAAGGCgccagc   >  1:187769/1‑138 (MQ=11)
                                                                                                                                          ttaAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATcc  >  2:923803/1‑143 (MQ=11)
                                                                                                                                              |                                                                                                                                          
TCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCC  >  NC_000913/2101607‑2101887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.