Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,050,410 A→T 6.2% S86T (TCG→ACG)  gcvT ← aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,050,4100AT 6.2% 201.2 / 2.1 65S86T (TCG→ACG) gcvTaminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base A (29/30);  new base T (2/2);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01

TTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAG  >  NC_000913/3050150‑3050684
                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                   
tttCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCCCACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCttt                                                                                                                                                                                                                                                                         >  1:405187/1‑273 (MQ=255)
                            gCGTCATTAAACAGGGGGGCAGCGTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGtt                                                                                                                                                                                                                                           <  2:37236/275‑1 (MQ=255)
                                 aTTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCGTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCca                                                                                                                                                                                                                                      <  1:131424/275‑1 (MQ=255)
                                          tgtgGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCCACTCGTTCGCCAGCAGATAAc                                                                                                                                                                                                                             >  2:312800/1‑275 (MQ=255)
                                          tgtgGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATATTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAAc                                                                                                                                                                                                                             >  1:201896/1‑275 (MQ=255)
                                              gCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAg                                                                                                                                                                                                                         <  2:155685/275‑1 (MQ=255)
                                               cAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGAAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGCGGCGGAAGACATCTTCCGTAAAGTAGTAGACGATGAGGTCATCTATCCACCCGCCAGAGGCATTCAACATCCCCGAATAAAGGGCTTTGCCACTCTTGGGGAGCTTCCCCACATCGTTCGCACGCAGATacaccaga                                                                                                                                                                                                                        >  2:428341/1‑268 (MQ=255)
                                                      tGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTgg                                                                                                                                                                                                                                                              >  2:219175/1‑230 (MQ=255)
                                                          tGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGTCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAg                                                                                                                                                                                                                                                          <  1:219176/230‑1 (MQ=255)
                                                            cgcATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGTGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTc                                                                                                                                                                                                                                                      >  1:357210/1‑232 (MQ=255)
                                                                ttCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGTGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCca                                                                                                                                                                                                                                                  <  2:357209/232‑1 (MQ=255)
                                                                         tGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGCAGAAATCTTAAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGa                                                                                                                                                                                                                                 >  1:421579/1‑240 (MQ=255)
                                                                             cGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAAc                                                                                                                                                                                                                             <  2:421578/240‑1 (MQ=255)
                                                                                ccaTCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATcgacg                                                                                                                                                                                       <  1:312801/273‑1 (MQ=255)
                                                                                cAATCATGGAAAGGTCATCACGGACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATcgacg                                                                                                                                                                                       <  1:188916/275‑1 (MQ=255)
                                                                                cAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATACCGc                                                                                                                                                                                                                           >  1:197327/1‑239 (MQ=255)
                                                                                cAATCATGGAAAGGCTATCACGAACGGTAATTTCGAGTCCGAGTGGTTAAGCGTGTTGGGGAATCCAGGAGATGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGGAGACGATGAGGTCATCTATCACACCGCAAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCTCCACATCGTTCGCCAGCAGATACCGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATcgacg                                                                                                                                                                                       <  1:443452/275‑1 (MQ=255)
                                                                                    cATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGaa                                                                                                                                                                                                                       <  2:197326/239‑1 (MQ=255)
                                                                                    cATGGAAAGGTCATCACGAACGGGAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGTGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATgg                                                                                                                                                                                   <  1:228110/275‑1 (MQ=255)
                                                                                          aaGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGTGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAACATcgacg                                                                                                                                                                                       >  2:228109/1‑265 (MQ=255)
                                                                                             gTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAAATCCCGGGTGCGGCCGCCGCGAAGACCGACGGTGGCCAAAtgt                                                                                                                                                                           >  2:50161/1‑274 (MQ=255)
                                                                                             gTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGAATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGACAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGAAGATGGTCATAtgt                                                                                                                                                                           >  1:447003/1‑274 (MQ=255)
                                                                                               catcaCGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTg                                                                                                                                                                                                                                                                        >  1:452858/1‑179 (MQ=255)
                                                                                               catcaCGAACGGTAATTTCGATGCCGAAAGGTTCAGCGGGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCGTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGa                                                                                                                                                                         <  1:445499/274‑1 (MQ=255)
                                                                                               aagcaCGAACGGTAATTTCGAGGCCGAAAGGGTCAGCGGGTTGGGGAATCCGGGAGAGGTCTTTTTCGCGGGTGGCGGAGTGAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGAGGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGac                                                                                                                                                                       <  2:405186/272‑1 (MQ=255)
                                                                                                   aCGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGc                                                                                                                                                                                                                                                                    <  2:452857/179‑1 (MQ=255)
                                                                                                               ttCGGTGCCGAAAGGTTCAGCGTGTTGGGTAATCCCGGCGAGGTCTGTTTCGCGGGTGGCGGAGTTAACAACTAGGCGGAAGAAAACTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCAGACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCgg                                                                                                                                                       <  2:420577/275‑1 (MQ=255)
                                                                                                                   aTGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACACCGTTCGCCAGCAGCTAACGCAGAAACTCCCGGTTCCGGCTGCCGCGAAGATCGACGCTGGTCATATGTGACCACACAAACATCACGACATc                                                                                                                                                   >  2:146342/1‑275 (MQ=255)
                                                                                                                       gggatgattCAGCGGGTTGGGTGAGCCAGGAGGGGTCTTTGTCGCGAGTGGCGGATGTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGGCGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTTGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGgt                                                                                                                                                <  1:146343/267‑1 (MQ=255)
                                                                                                                                               tCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGACGATCGCCGATGGTCATATGTGACACATCAAACATTCCGGCATAGGTACGTACCGCATGATGTTCGCCGAtt                                                                                                                       >  2:74257/1‑275 (MQ=255)
                                                                                                                                                    gagaGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGCAGATAGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATTTTCGTCGATTTGcca                                                                                                                  >  1:443872/1‑273 (MQ=255)
                                                                                                                                                    gagaGGTCGTGTTCGCGGGGGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGAGGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCg                                                                                                                   <  1:89823/274‑1 (MQ=255)
                                                                                                                                                         gTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTCCCGCCTGATGTTCGTCGCTTTGCGAACCg                                                                                                              >  1:357553/1‑274 (MQ=255)
                                                                                                                                                         gTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGt                                                                                                             >  2:188141/1‑275 (MQ=255)
                                                                                                                                                                   cgGGTGGCGGAGTTAAAAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGGAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAg                                                                                                      <  1:523388/272‑1 (MQ=255)
                                                                                                                                                                                      cGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGcc                                                                                                                                                                                                                                                                      >  2:228455/1‑94 (MQ=255)
                                                                                                                                                                                         ggCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATc                                                                                                                                                                                           >  1:144871/1‑166 (MQ=255)
                                                                                                                                                                                          gCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCtt                                                                                                                                                                                                                                                                  <  1:228456/94‑1 (MQ=255)
                                                                                                                                                                                             gaagaaATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATcgacg                                                                                                                                                                                       <  2:144870/166‑1 (MQ=255)
                                                                                                                                                                                                  aaTCTTTAGTAAAGTAGTACGCGATGAGGTCATCTATCACGCCGCCAGAGGCATTCAACTTCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGa                                                                    <  1:428342/275‑1 (MQ=255)
                                                                                                                                                                                                    tCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAgc                                                                  <  1:188142/275‑1 (MQ=255)
                                                                                                                                                                                                    tCTTCAGTAAAGTAGTAGACGAGGAGGTCATCTATCACACCGCCAGGGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGGGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAgc                                                                  <  2:201895/275‑1 (MQ=255)
                                                                                                                                                                                                        cAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGGGGAAATCCACCATGCGAGcgccg                                                              >  2:253775/1‑275 (MQ=255)
                                                                                                                                                                                                              aggagtagACGATGAGGTCATCTAGCACACCGCCATAGGCATTCAACATCCCCGAGTAAAGGGCGTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACAGCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGc                                                        <  1:81360/272‑1 (MQ=255)
                                                                                                                                                                                                                     gACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATAAAACATTCCGGCATCGGTACGTACAGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCCTTAACCAGCCGTGGGAATCCACAACGCGCGCGCCGCaaatagggtggt                                                 >  1:311187/1‑266 (MQ=255)
                                                                                                                                                                                                                       ggATGAGGTCAGCTATCACACCGCCAGCGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGGGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCAGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGtgttgt                                               <  1:400745/274‑1 (MQ=255)
                                                                                                                                                                                                                        gaggAGGTCATCTATCACACCGCCAGGGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGACAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGtgttgt                                               <  2:447002/271‑1 (MQ=255)
                                                                                                                                                                                                                          tGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGACGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGAATCATCCAGCCGGGGAACTCCACCATGCGCGCGCCGCACAGCGGGGTTTGTTCg                                            >  1:241168/1‑275 (MQ=255)
                                                                                                                                                                                                                          tGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTTGGTTGTTCg                                            >  2:123295/1‑275 (MQ=255)
                                                                                                                                                                                                                                        acacCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGAtt                                                                                                                       >  1:7201/1‑186 (MQ=255)
                                                                                                                                                                                                                                         cacCGCCAGAGGCCTTCACCACCCCCGAGTAAAGGGCGTGGCCGCTTTTGGTGAGGTTGGCCACATCGTTCGCCAGCAGATAACGCAGAAACGCCCGGGTGTGGCTGCCGAGAAGATCTACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCtg                              <  2:357044/274‑1 (MQ=255)
                                                                                                                                                                                                                                         cacCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACCAATCAAACATTCCGGCATCGGTACGTACCGCATTATGTTCGTCGATTTGCGAACCGTAATTCAGCGGCATCATCCCGCCGTGGAAATCCCCCATGCGAGCGCCGCAACGCGTGTGTTGTTCGTACAAAGGAGTTtg                              >  2:74685/1‑274 (MQ=255)
                                                                                                                                                                                                                                         cacCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACTGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATTCGAGCGCCGCAAAGCGTGTGTTTTTCGTACAACGGAGTCtg                              >  2:83637/1‑274 (MQ=255)
                                                                                                                                                                                                                                           ccGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTTTTCGTACCAAGGGGGCtgtt                            >  2:139638/1‑274 (MQ=255)
                                                                                                                                                                                                                                            cGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCg                                                                                                                   <  2:7201/186‑1 (MQ=255)
                                                                                                                                                                                                                                             gCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTAGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACTGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCtg                              <  1:83638/270‑1 (MQ=255)
                                                                                                                                                                                                                                              ccAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCCTCGGTACGTACCGCATGATTTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCACCCAGCCGAGGAAATCCCCCATGCGCACGACGCCAAGCGTGTGTTGTTCGTACAAAGGAGACtgtt                            >  2:410218/1‑271 (MQ=255)
                                                                                                                                                                                                                                              ccAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGTGTCTGTTGTGc                        >  1:158933/1‑275 (MQ=255)
                                                                                                                                                                                                                                              ccAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGAGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTtgtg                         <  1:50161/274‑1 (MQ=255)
                                                                                                                                                                                                                                               caagggCATTCAACACCCCCGGGTAAAGGGCTTGGCCGCTTTTGGTGGGCTTCTCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGGGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTtgtg                         <  1:139639/269‑1 (MQ=255)
                                                                                                                                                                                                                                                     cATGCAACCTCCCCGAGTAAATGGCTTTGCCGCTTGTTGTGAGCTTCGCCACATCGTTCGCCAGAAGAAAACGCGGAAACTCCCTGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGTCATCGGTACGTACCGCATAATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTg                 <  1:410219/275‑1 (MQ=255)
                                                                                                                                                                                                                                                      ttccacccccccgGAGTAAAGGCCTTTGCCCGTGTGGGGCGGTTTCGCAACACCGTTCGCCAGCAGATAACGCAGAACCTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATTTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGt                <  2:311186/266‑1 (MQ=255)
                                                                                                                                                                                                                                                      aTTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCACAGCGTGTGTTTTTCGTACAAAGCAGTCTGTCTTGCcttcttc                 >  1:423219/1‑273 (MQ=255)
                                                                                                                                                                                                                                                      aTTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCACCCGTGGAAATCCACCATTCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTTCCCTCTTg                 >  2:250644/1‑274 (MQ=255)
                                                                                                                                                                                                                                                             aTCCCCGGGTAAAGGGCTTTGCCGCTTTGGGTGGGCTTGGCAACATCGTTCGCCAGCAAATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCAt          <  2:423218/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                    aGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCCTCTTTTCCTCATTGAATAAg  >  2:217714/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                    aGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAg  >  2:100809/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                   
TTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAG  >  NC_000913/3050150‑3050684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: