Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	702,306	C→T	R149H (CGT→CAT)	nagA ←	N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	702,306	0	C	T	100.0%	142.6 / NA	45	R149H (CGT→CAT)	nagA	N‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/20);  total (25/20)

GTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATC  >  NC_000913/702167‑702434                                                                                                                                            |                                                                                                                                 gTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACCCACAAAATCGACCAGCGCGGCATCAGGCTTATGca                                                                                                                               <  1:326722/143‑1 (MQ=255)         cAGAAACCACAATCCCGGCATTTGCCAGTTTGATGATGACTCCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCCTCCGGCTTATGCACAAAATTc                                                                                                                       >  2:185842/1‑143 (MQ=255)          agcaaCCACGATCCCGGCATTGTCCAGTTTGCTGATGACGTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCg                                                                                                                      <  1:149711/140‑1 (MQ=255)          aGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCACCGGGCTTATGCACACAATTCg                                                                                                                      >  2:166527/1‑143 (MQ=255)            aatcctcaATCCCTTTATTTTCCAGTTGTCTTATGACGTTCGCAGGATCCATTTTCGTTGTCAGGGTCACTTTGGTAATGATGTCGGCGTTTTCACACAGGAAATCGATCAGCGCGGCATCAGGCTTATGCACAAAATTCGGa                                                                                                                    <  2:303648/137‑1 (MQ=255)                cacaATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTAt                                                                                                                >  1:185343/1‑143 (MQ=255)                   aaTCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGCATTATggg                                                                                                             >  1:607120/1‑143 (MQ=255)                        cGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCt                                                                                                        >  1:497213/1‑143 (MQ=255)                             tttGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCtttttt                                                                                                   <  1:586172/143‑1 (MQ=255)                                gCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAACCTTGGGATTATGGGTGGCTTTTTTTAc                                                                                                >  1:154417/1‑143 (MQ=255)                                    gTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGa                                                                                            <  1:617040/143‑1 (MQ=255)                                           gatgaCTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGcc                                                                                     >  2:54230/1‑143 (MQ=255)                                           gatgaCTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTGTTTTACCAGATTCCGcc                                                                                     >  2:665372/1‑143 (MQ=255)                                            atgaCTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCa                                                                                              >  1:110541/1‑133 (MQ=255)                                            atgaCTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCa                                                                                              <  2:110541/133‑1 (MQ=255)                                             tgaCTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCAc                                                                                   <  1:148012/143‑1 (MQ=255)                                                     gCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGAAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGAGTATGGGTGCCTGTTTTTACCAGATTCAGCCACGGACCGTc                                                                           >  2:709696/1‑143 (MQ=255)                                                       aGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCa                                                                         <  1:714041/143‑1 (MQ=255)                                                         gAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGCTTAAGCCACGGACCTTCCAGa                                                                       >  2:394532/1‑143 (MQ=255)                                                          acccATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGCTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGAt                                                                      <  2:326052/141‑1 (MQ=255)                                                           aCCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCCGATTCGGCCACGGACCTTCCAGATg                                                                     >  1:765654/1‑143 (MQ=255)                                                           aCCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATg                                                                     >  1:860202/1‑143 (MQ=255)                                                             cATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTCTGGGTGCCTTTTTTTACCAGATTCGGCCACGGACCGTCGAGATGCa                                                                   >  1:62427/1‑143 (MQ=255)                                                             cATGTCCGGTGGCAGGGTCACTGTGGGCGTGACGTCGGCGTTTTCGCACAGGAGATCGACCAGCGCGGCATCGGGCTTATGCACAAAATTCGGCTACTGCCAACTTTTGTTTACCAGATTCAGCCACGGACCTTCCAGATGCa                                                                   <  2:386405/143‑1 (MQ=255)                                                               ttcccGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGa                                                                 <  1:799955/140‑1 (MQ=255)                                                                  ccGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCt                                                              >  1:392/1‑143 (MQ=255)                                                                                     ggTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGtt                                           <  1:458537/143‑1 (MQ=255)                                                                                           gACGTCGGCGTTTTCACACAGGAGATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCa                                     <  2:200896/143‑1 (MQ=255)                                                                                           gACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCa                                     <  2:257359/143‑1 (MQ=255)                                                                                            aCGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAg                                    >  1:831054/1‑143 (MQ=255)                                                                                            aCGTCGGCGTTGTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTGTTTACCAGAGTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAg                                    <  1:129009/143‑1 (MQ=255)                                                                                             cGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAgg                                   >  1:332135/1‑143 (MQ=255)                                                                                                       ttCACACAGGTCAGCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCa                         <  2:303242/143‑1 (MQ=255)                                                                                                       ttCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCa                         >  2:564546/1‑143 (MQ=255)                                                                                                           cacaGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAAc                     <  1:338345/143‑1 (MQ=255)                                                                                                             caGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCCTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCGCGGCACCCGCGCATAAcgc                   >  1:534669/1‑143 (MQ=255)                                                                                                              aGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGa                                                     <  1:649577/108‑1 (MQ=255)                                                                                                              aGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGa                                                     >  2:649577/1‑108 (MQ=255)                                                                                                                gAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCa                >  1:419369/1‑143 (MQ=255)                                                                                                                gAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCa                >  1:257706/1‑143 (MQ=255)                                                                                                                     cgccccgcgcGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCTAGATTCAGTCCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGccc           <  1:544114/137‑1 (MQ=255)                                                                                                                        ccggcgcGGCGTCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGCCCTTCCAGATGCAGCCCGAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGt        <  2:767902/140‑1 (MQ=255)                                                                                                                           gcgcGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCAAGCCCTGATtc     >  1:251459/1‑143 (MQ=255)                                                                                                                           gcgcGGCATCAGGCTTATGCACAAAATTCGGATTATGGGCGCCTTTGTTTACCAGAGTAAGTCACGGACCTTACCGATGCAGACCTAACGCCTGATTCGGATGTTTTAGCAGGTACTCACCAATAGCGCGCAGGCGCTggctg     >  2:733743/1‑139 (MQ=255)                                                                                                                              cgGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTtcatc  >  2:471438/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                 GTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATC  >  NC_000913/702167‑702434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑