Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258674 258683 10–776 80 [0] [0] 80 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)74 (1.190) 58/266 0.0 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

GTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTG  >  NC_000913/258542‑258824
                                                                                                                                              |                                                                                                                                            
tgtcaaaaatttgctaacttttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgtgtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaa                                                                                                                                              <  2:416983‑M2/1‑1 (MQ=255)
tggtaaaaatcttctagattttcccaatgtcgtacaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacccccgaatagcagattgatcaaaaaatttaccgccctaggcccgtatattcgtgaaggtaa                                                                                                                                              <  2:1186365‑M2/1‑1 (MQ=255)
  ttaaaaattggctaaatgttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGt                                                                                                                                            <  1:1011685‑M2/3‑1 (MQ=255)
             ctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAAt                                                                                                                                 <  1:157607‑M2/14‑1 (MQ=255)
              taaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGataagg                                                                                                                                >  1:257757‑M2/129‑141 (MQ=255)
              taaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATc                                                                                                                                >  1:130796‑M2/129‑143 (MQ=255)
                aattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGa                                                                                                                              >  2:901951‑M2/127‑143 (MQ=255)
                aattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtagattcgtgaaggtaAGTGCAAAGATAATCGa                                                                                                                              >  2:1116264‑M2/127‑143 (MQ=255)
                     ggccaatgtggtaaaacaggtgcagcacgacaggagatagcaatgacgttaccgaggggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatagtcgcgaaggtaAGTGCAAAGATAATCGATTCtt                                                                                                                         <  2:654560‑M2/22‑1 (MQ=255)
                      gccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtctattcgtgaaggtaCGTGCAAAGATAATCGCTTCttt                                                                                                                        >  2:965369‑M2/121‑143 (MQ=255)
                             tggtaagacagtggcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatatccgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                                                                 <  2:807736‑M2/30‑1 (MQ=255)
                              ggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTg                                                                                                                >  2:481254‑M2/113‑143 (MQ=255)
                                taaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtacattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                                                              >  1:1113660‑M2/111‑143 (MQ=255)
                                taaaacagtggcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                                                              <  2:773089‑M2/33‑1 (MQ=255)
                                  aaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTg                                                                                                            >  1:1158874‑M2/109‑143 (MQ=255)
                                   aacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGGAAAGATAATCGATTCTTGTTCGATTGTCTgg                                                                                                           >  1:956605‑M2/108‑143 (MQ=255)
                                       gttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGt                                                                                                       >  1:645876‑M2/104‑143 (MQ=255)
                                        ttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                                                                      >  1:662295‑M2/103‑143 (MQ=255)
                                            atcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgctcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCGTTTTCGATTGGCTGGCTGTATGCg                                                                                                  >  1:1098387‑M2/99‑143 (MQ=255)
                                             tcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATGGTCTGGCTGTATGCGt                                                                                                 >  2:5168‑M2/98‑143 (MQ=255)
                                                caacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCaa                                                                                              >  2:80893‑M2/95‑143 (MQ=255)
                                                 aacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAAc                                                                                             >  2:1029673‑M2/94‑143 (MQ=255)
                                                 aacaggagatagaaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAAc                                                                                             <  1:1003582‑M2/50‑1 (MQ=255)
                                                     ggagacagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcgcgtatagtcgtgtaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGa                                                                                         <  2:842392‑M2/54‑1 (MQ=255)
                                                         atagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                                                                     >  1:626260‑M2/86‑143 (MQ=255)
                                                           atcaatgacgtcaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATATCCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                                                                   <  2:361596‑M2/60‑1 (MQ=255)
                                                           agcaaggacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTGTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                                                                   <  2:427819‑M2/60‑1 (MQ=255)
                                                                   cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgggaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCCCAGGaa                                                                           >  2:674883‑M2/76‑143 (MQ=255)
                                                                     ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAGTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                                                         >  2:166510‑M2/74‑143 (MQ=255)
                                                                     ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                                                         >  2:252385‑M2/74‑143 (MQ=255)
                                                                     ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                                                         <  1:73807‑M2/70‑1 (MQ=255)
                                                                       accgagtggacacccgaagagcagattgatcaaaaaatttcccgccctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGc                                                                       <  1:380346‑M2/72‑1 (MQ=255)
                                                                        ccgagtggacacccgaggggcagattgatcaaaaaatttaccgcactaggccggtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTGCGATTGTCTGGCTGTATGCGGCAACGTGAAACCGGCACCGGAAGTGCg                                                                      <  2:1022974‑M2/73‑1 (MQ=255)
                                                                         cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                     <  1:929823‑M2/74‑1 (MQ=255)
                                                                         cgagtagacaccagaagagaagattgatcaaaaaatttaccgtactaggcccgtatattcgtgaaggtaAGTGAAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                     <  1:120579‑M2/74‑1 (MQ=255)
                                                                                 cacgcgaagagcagattggtcaagaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                                                             <  2:676624‑M2/82‑1 (MQ=255)
                                                                                    ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                                                          >  1:935692‑M2/59‑143 (MQ=255)
                                                                                     cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAACGGCACCGGAAGTGCGTGAATTCTGGGGc                                                         >  2:262491‑M2/58‑143 (MQ=255)
                                                                                     cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTACAACTATAATCTCTTCTTTTTCGATTGTCTGGCTGTATGCGTCCACGTGAACCCGCCACCCGAAGTGCCTGACGTCTCGGGc                                                         >  1:445577‑M2/58‑143 (MQ=255)
                                                                                         gagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAGTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggt                                                     <  2:472105‑M2/90‑1 (MQ=255)
                                                                                          ggcaggttgatcaaaaaagttaccgcactaggcccgtatattcgtgcaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg                                                    <  2:192345‑M2/91‑1 (MQ=255)
                                                                                          agcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTACAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg                                                    <  1:1035514‑M2/91‑1 (MQ=255)
                                                                                          agcagattctgcaacaaacttaccgcacgaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTGTTTCGATTGGCGGGCTGTATGCGTCAACGTGAACCCGGCACCGGAAGTGCGTGAATTCTGGGCCtgttg                                                    <  2:442953‑M2/91‑1 (MQ=255)
                                                                                           gcagatttatcaaaaaatttaccgcactaggcccgtagattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtgg                                                   <  1:990109‑M2/92‑1 (MQ=255)
                                                                                           gcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAGTCTGGGGCtggtgg                                                   >  2:563458‑M2/52‑143 (MQ=255)
                                                                                              gattgatcaaaaaatttaccgcactaggcccgtatagtcgtgaatgtaAGGGCAAAGATAGTAGAGTCTGTTTCGGTTGTCTGGCTGTATGCGTCCACGTGAAACCGGCACCGGAAGGGCGTGAGTTCTGGGTCTGGgggttg                                                >  1:167874‑M2/49‑143 (MQ=255)
                                                                                                 tgatcaaaaaatttaccgcactaggcccgtatattcgtgaaagtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATCGAg                                             >  2:1186621‑M2/46‑143 (MQ=255)
                                                                                                   atcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTCTGCGCGTACGGGGAACCGGCCCCGGTAGTGCGTGAATTCTGGGGAcggtggacggacca                                           >  1:991208‑M2/44‑140 (MQ=255)
                                                                                                      aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGGAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGa                                        >  1:823739‑M2/41‑143 (MQ=255)
                                                                                                      aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGCGCTGGa                                        >  2:165539‑M2/41‑143 (MQ=255)
                                                                                                      aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGa                                        >  2:1079449‑M2/41‑143 (MQ=255)
                                                                                                      aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGa                                        >  1:974296‑M2/41‑143 (MQ=255)
                                                                                                       aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGaa                                       <  1:1029673‑M2/104‑1 (MQ=255)
                                                                                                       aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGaa                                       >  2:807557‑M2/40‑143 (MQ=255)
                                                                                                       aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAACCGATTCTTTTTCGATGGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTCCGTGAATTCTGGGGCTGGTGGGTGGAGctggca                                       >  2:213905‑M2/40‑141 (MQ=255)
                                                                                                         aaattcaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGGATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgc                                     <  1:718509‑M2/106‑1 (MQ=255)
                                                                                                           tttgaccgctccaggcccttatatgcgtgaagttaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTATGGGGCTGGTGGATGGAGCTGGAAgcgc                                   <  2:722936‑M2/108‑1 (MQ=255)
                                                                                                            tttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                  <  1:481254‑M2/109‑1 (MQ=255)
                                                                                                             ttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAg                                 <  1:653985‑M2/110‑1 (MQ=255)
                                                                                                                 cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCTGGTGGATGGCGCAGGAAGCGCAGGAAt                             >  1:359161‑M2/30‑143 (MQ=255)
                                                                                                                    tctttgcccgtatattcttgtaggtaAGTTCAAATATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATccc                          <  2:970661‑M2/117‑1 (MQ=255)
                                                                                                                    actaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCGGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGAGGGAGCTGGAAGCGCAAGAAcccc                          >  2:591132‑M2/27‑143 (MQ=255)
                                                                                                                     ctaggcccgtatattcgtgaaggtaGGTGCAAAGATAATCGATTCTGTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCg                         <  2:892950‑M2/118‑1 (MQ=255)
                                                                                                                         gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAACCCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtttt                     <  2:445790‑M2/122‑1 (MQ=255)
                                                                                                                         gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCCGGAATCCCGtttt                     >  2:177816‑M2/22‑143 (MQ=255)
                                                                                                                         gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtttt                     >  1:652274‑M2/22‑143 (MQ=255)
                                                                                                                          cccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATGCTGGGGCTGGTGGATGGAGGTGGAAGCGGAGGAAGCCCGTTTTa                    >  2:760453‑M2/21‑143 (MQ=255)
                                                                                                                           ccgtatattcgtgaaggtaAGTGCAAAGATAATCGCTTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGCATGGAGCTGGAAGCGCAGGAATCCcgttttgc                   >  2:1077101‑M2/20‑141 (MQ=255)
                                                                                                                           ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGCAGCCCGTTTTAc                   >  1:101822‑M2/20‑143 (MQ=255)
                                                                                                                           ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                   <  1:1094953‑M2/124‑1 (MQ=255)
                                                                                                                                tattcgtgaaggtaAGGGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGGGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa              <  1:901951‑M2/129‑1 (MQ=255)
                                                                                                                                 attcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGCGCTGGaa                                       >  2:506637‑M2/14‑117 (MQ=255)
                                                                                                                                 attcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGaa                                       <  1:506637‑M2/104‑1 (MQ=255)
                                                                                                                                  ttcgtgaaggtaAGTGCAAAGATAATCGAGTCTGTTTCGAGTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGt            <  2:130796‑M2/131‑1 (MQ=255)
                                                                                                                                       gaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGGGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCa       <  1:1063933‑M2/136‑1 (MQ=255)
                                                                                                                                        aaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACTAg      >  1:850977‑M2/7‑143 (MQ=255)
                                                                                                                                           gtaGGTGCAAGGATAATCGATTCTTTTTGGATTGTCTTGCTGTATGCGTCAACGTGAAACCGGCAGCGGGAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGttt   <  2:1113660‑M2/140‑1 (MQ=255)
                                                                                                                                           gtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGttt   <  2:174807‑M2/140‑1 (MQ=255)
                                                                                                                                            taAGTGCACAGCTACTCGATTCTCTTTCGATTGTCTGGCTGTATGCGTCAACGCGAATCCGGCACCGGAAGTGCGTGAATTCTTGGGCTGGTGGATGGAGCTGGAATCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg  <  2:1098387‑M2/141‑1 (MQ=255)
                                                                                                                                            taAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg  <  1:1096726‑M2/141‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                            
GTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTG  >  NC_000913/258542‑258824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: