Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	702,066	G→C	P229R (CCT→CGT)	nagA ←	N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	702,066	0	G	C	100.0%	115.5 / NA	40	P229R (CCT→CGT)	nagA	N‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (23/17);  total (23/17)

GGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTT‑GGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTT  >  NC_000913/701927‑702204                                                                                                                                             |                                                                                                                                           ggTGGCCGCAGTAACCTGACAAAAAATGACGACTTTCAGACGTTTAGCGTTGCGAATGTT‑GGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGtt                                                                                                                                         >  2:639869/1‑143 (MQ=255)         cAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTT‑GGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCg                                                                                                                                 >  2:205869/1‑143 (MQ=255)               ccAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTT‑GGCGTAATCAACATGCAGGCCATCCGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTGCACGACCGGTAata                                                                                                                           >  1:19200/1‑143 (MQ=255)                cctccacaGTTTGTCGCCTTTCAGCCGTGTACCTTTGCGAATGTT‑GCCGTAATCAACATGCAGGCCATTAGCAATGATACCGCAATACATGTCAGCTTCGTAGAGGATCGCGCCCGCCAGCCCACGTTCACGACCGGTAatat                                                                                                                          <  2:686591/139‑1 (MQ=255)                        gttcgctgCCTCTCAGCCGTTGAGCGTCG‑GTACGTTGGGCGGAATCAACATGCCTGCCATCAGCAATAATACCGCAATCATTGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCTACGTTCACGACCGGTAATATACGGCATc                                                                                                                  <  2:194359/136‑1 (MQ=255)                           tGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTT‑GGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATcgcg                                                                                                               >  1:525034/1‑143 (MQ=255)                                ccTTTCAGACGTTTAGCGTTGCGAATGTT‑GGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCAGCGCGTTGTa                                                                                                          >  1:478313/1‑143 (MQ=255)                                   ttCAGACGTTTAGCGTTGCGAAGGTG‑GGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAg                                                                                                       <  1:34369/143‑1 (MQ=255)                                     cAGACGTTTAGCGTTGCGAATGTT‑TGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCTAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGAt                                                                                                     <  1:329256/143‑1 (MQ=255)                                         cGTTTAGCGTTGCGAATGTT‑GGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGt                                                                                                 <  1:771491/143‑1 (MQ=255)                                               gCGTGGCGAGTGTT‑GGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCaaa                                                                                           <  1:27556/143‑1 (MQ=255)                                                cGTTGCAAATGTT‑GACTTAATCAACATGCAGGTCATACGCGATAATACCGCAAGAAATGGGAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATCCGGCATCGCGTTGTACAGATGGGTGGCAAAg                                                                                          <  1:288653/143‑1 (MQ=255)                                                  ttGCGAATGTT‑GGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGt                                                                                        <  1:754762/143‑1 (MQ=255)                                                   tccGAATGTT‑GGCGTAATCAACATGCAGGCCATCATCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTa                                                                                       <  1:237842/141‑1 (MQ=255)                                                          tgtggtcGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGGCGAGGATCGCGCGCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATcccc                                                                                 <  2:174401/137‑1 (MQ=255)                                                              ggCGTCATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCcgcgc                                                                             <  1:298796/143‑1 (MQ=255)                                                                  tAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATCTAAGACAGCACGTAGTACCGATGGGTGGGAAAGGTGATCCCCGCGCGGaa                                                                         >  2:522158/1‑143 (MQ=255)                                                                    aTCAACATGCAGGCCATCAGCAATAATACCGCAATAGATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAGTCCCCGCGCGGAAAc                                                                       >  1:571011/1‑143 (MQ=255)                                                                       aaCATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGGTCACGACCGGTAATATACGGCATCGCGAGCTAGAGATGCGGGGCCAAGCTCCTCCCCCCGCGGCAAccag                                                                    >  1:812774/1‑141 (MQ=255)                                                                       aaCATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCAGCCAGGCCACGTTCACGACCGGTAATATAAGGCATCGCGTTGTACAGGTGGGTGGAAAAGGTAATCAGCGCAGGGaaagtgg                                                                    >  1:320228/1‑139 (MQ=255)                                                                        aCATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATGGCGCCCGCCAGGCCACGCTCACGACCGGTAATATAAGGCAGCGCCTGGGCCAGATGGGGGGCCAGCGCAATCCGCGCGGCGGAACCGGc                                                                   >  2:877856/1‑143 (MQ=255)                                                                              aGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCAAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGGACATGTGGGTGGCAAAGGTACCCCCCGCGCGGAAACCGGCTTTTGc                                                             >  1:862494/1‑143 (MQ=255)                                                                                   atcatcAATAATACCTATATAATTTTTTGTTTCGTTGAGGTTCTCGCCCGCCAGGCCACGTTCACGACCGGTAACATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGcttctt                                                        <  1:79800/143‑1 (MQ=255)                                                                                    tCAGCACTAATGCCGTGAGGAATGGCAGCTGCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCttt                                                       <  2:805557/143‑1 (MQ=255)                                                                                    tCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCttt                                                       >  1:882420/1‑143 (MQ=255)                                                                                          ataataCCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGt                                                 >  1:113520/1‑143 (MQ=255)                                                                                               aCCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGAAATCCCCGGGCGGGAACCGGCTTTTGCTTCTTACAACGACGCGt                                            >  1:479489/1‑143 (MQ=255)                                                                                                 cGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGCTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCGCTTGCTTCTTTCGACGGCGCGtgg                                          >  2:282330/1‑141 (MQ=255)                                                                                                   cgaTACATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGa                                        <  2:876895/141‑1 (MQ=255)                                                                                                   cAATAACTGTCAGCTCCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGGCCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCCTCGTCGCGTTGGa                                        <  2:537460/143‑1 (MQ=255)                                                                                                   cAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGCTGGGGGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGCTGGa                                        >  1:719351/1‑143 (MQ=255)                                                                                                    aaTAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGCGGCAACTGGAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTGGGAg                                       >  2:101512/1‑143 (MQ=255)                                                                                                             cAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGTCCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGATAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCa                              <  1:555588/143‑1 (MQ=255)                                                                                                               gCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGGTGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGGCCGGCAGa                            >  1:70711/1‑143 (MQ=255)                                                                                                                    gtcgAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACca                       <  1:369940/143‑1 (MQ=255)                                                                                                                     tcgAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACcac                      >  2:32235/1‑143 (MQ=255)                                                                                                                       gAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGGAACCACaa                    >  1:63562/1‑143 (MQ=255)                                                                                                                            tCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGATGGCAAAGGTCATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCg               >  1:354010/1‑143 (MQ=255)                                                                                                                              gcgcCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCgtg             >  1:276697/1‑141 (MQ=255)                                                                                                                                  ccgccAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCAttt         >  1:255366/1‑143 (MQ=255)                                                                                                                                      cagggcacgTTCACGGCCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCACAGGCAATCCCCGCGCGGCAACCCGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCa     <  2:785338/143‑1 (MQ=255)                                                                                                                                         gccacgTTCACGGCCGGTAATATACCGCATCGCGTTAAACAGTTTTGGGTCAAAGGTAAGCCCCGCGCGGAACCCGGCCTTTGCTTCTTTCCACGGCGCGTTGGAGTGACCGGCAGAAACCACAAGCCCGGCATTTGCCAGtt  <  2:619499/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                           GGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTT‑GGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTT  >  NC_000913/701927‑702204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑