Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA97.9% 138.2 / NA 48P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (23/24);  minor base G (1/0);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

GGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184409‑4184681
                                                                                                                                      |                                                                                                                                          
ggTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACGGAACCAGCTGGGCg                                                                                                                                    >  1:339902/1‑143 (MQ=255)
ggTCAAACGCCGCATCCAGCCGGGTGACATGATCGATGGATGTGACATTGGCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCg                                                                                                                                    <  1:400779/143‑1 (MQ=255)
 gTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGt                                                                                                                                   >  2:727352/1‑143 (MQ=255)
 gTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGGCGACATCGTACTGAACCAGCTGGGCGt                                                                                                                                   >  2:728610/1‑143 (MQ=255)
     aaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTACGATGCTCCCGATCCACGCCATGCCTTACGAGGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCg                                                                                                                               <  1:878133/143‑1 (MQ=255)
       cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTCCTGAGCCAGCTGGGCGTACCGtc                                                                                                                             >  2:483469/1‑143 (MQ=255)
          cGTATCCAGCCTGGTGACAAGATGGGAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCg                                                                                                                          <  2:645754/143‑1 (MQ=255)
             ctCCAGCCAGTTGACAAGACGGCAGGTGGTCACGGTAACAAGGGTGTAATGTCTAAGATCACCCCGATCGAAGATATGACTTACGATGAAAACGGCACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                       <  1:354360/142‑1 (MQ=255)
              tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGGAATTTCTAAGATCAACCCGATGGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                      <  1:876148/143‑1 (MQ=255)
                      ggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCgg                                                                                                              >  1:52183/1‑143 (MQ=255)
                          aCAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAg                                                                                                          >  2:875056/1‑143 (MQ=255)
                            aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTCCCGTCTCGTATGAACGTCGGTCAGAt                                                                                                        >  2:519069/1‑143 (MQ=255)
                                   cAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaa                                                                                                 >  2:647278/1‑143 (MQ=255)
                                   cAGGGCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaa                                                                                                 <  2:125318/143‑1 (MQ=255)
                                      gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACCGCGGTAGACATCGTACTGAACCAGCTGGGGGTACCGGCACGGATGAACATCGGTCAGATCGCGGAAAcc                                                                                              >  2:789695/1‑143 (MQ=255)
                                         gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCCGATCCTCGAAACCCAc                                                                                           >  1:459186/1‑143 (MQ=255)
                                          tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATTGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAGCATCGGTCAGATCCTCGAAACCCAcc                                                                                          >  1:674823/1‑143 (MQ=255)
                                                 aaCAAGGGTGTCATTTCTAAGATCACCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCCGTAGACATCGTACTGAATCAGCTGTGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAt                                                                                   <  1:643723/143‑1 (MQ=255)
                                                 aaCAAGGGTGTAATTTCTAAGAGCAACCCGATCGAGGATATGCCTTACGATGAAAACTGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAt                                                                                   <  1:206230/143‑1 (MQ=255)
                                                    aggggTGTAATTTCTAAGATCAACCCGATCGAAAATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTAGGCGTACCGTCTCGAATGAACATCGGACAGATCCTCGAAACCCACGCGGGTATGGc                                                                                >  2:723141/3‑143 (MQ=255)
                                                    aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGc                                                                                <  1:628238/143‑1 (MQ=255)
                                                       ggTGTGATGTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGc                                                                             <  1:240859/143‑1 (MQ=255)
                                                                  tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg                                                                  >  2:30544/1‑143 (MQ=255)
                                                                  tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTAGAAACACACCTGGGTATGGCTGCGAAAGGAAtag                                                                  >  1:370278/1‑141 (MQ=255)
                                                                  tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCCGTAGACATCGTACTGAACCGGCTGGGCGTACCGTGTCGTATGCACATCGGTCAGATCCTCCAAACCCACCGGGGCATCGCTCGGCAAGGTATcc                                                                  >  2:713872/1‑142 (MQ=255)
                                                                   cagagctaCCCCATCTAAGATATGCCCTACGAGCAAACCGGTTCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCgg                                                                 <  2:578567/136‑1 (MQ=255)
                                                                      aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTACCGGCGa                                                              >  2:158246/1‑143 (MQ=255)
                                                                      aTCAACCCGATCGAAGATATGCCGTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                              <  1:127884/143‑1 (MQ=255)
                                                                        cAACCCGAGCGAAGATATGCCTTAACATGAACACGGGACGCCGGTAGAGATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACGTCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACa                                                            <  2:833565/143‑1 (MQ=255)
                                                                                tgtaaGATATGCCTTACGATGAGAACGGTTCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGGATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                    <  2:72737/140‑1 (MQ=255)
                                                                                 cGAAGATATGCCTTACGCTGGACACGGTACGCCGGTAGACATCGTACTGGACCAGCTGGGCGTACCGCATCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACg                                                   <  2:263500/143‑1 (MQ=255)
                                                                                  gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCCGCGAAAGGTATCGGCGACCAGATCAACGc                                                  >  2:833337/1‑143 (MQ=255)
                                                                                  gAAGAGATGCCGGACGATGAAAACGGTACGCAGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAGCGGTCAGATCCTCGAAACCCACCTCGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGc                                                  <  1:72554/143‑1 (MQ=255)
                                                                                    ctatatGCCTGACGTTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCa                                                <  1:542630/141‑1 (MQ=255)
                                                                                             ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCGACGAAAGGTCTGGGCGCCAAGGTCAACGCCATGCTGAAAc                                       >  1:784312/1‑143 (MQ=255)
                                                                                                 gaggAAGACGGCACGCAGGTAGAGTGCGTACGGAACCAGCTGGGCGTACCGTCGCGTATGAAGATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                   <  1:402580/140‑1 (MQ=255)
                                                                                                 gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                   >  2:472888/1‑143 (MQ=255)
                                                                                                 gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGCTGGAACAACCGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                   <  1:641293/143‑1 (MQ=255)
                                                                                                  aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCTTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagcag                                  <  1:433929/143‑1 (MQ=255)
                                                                                                   tGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGCAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc                                 >  2:479879/1‑143 (MQ=255)
                                                                                                      caagtgTAGGCCGGGAGATAAGGTGCTGAACCAGCTGGTCTTACTGTCGCGTAGGAACAGCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaag                              <  2:259357/138‑1 (MQ=255)
                                                                                                             gcGCCGGTAGACAGCGCACTGAACCAGCTGGGCGTACCGGCTCGTATGAATATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                       <  2:681896/142‑1 (MQ=255)
                                                                                                              cGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCCCGCACCCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCGCAAACATCGGCAAGACGTcgcg                      >  1:55809/1‑143 (MQ=255)
                                                                                                                 cGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAACGGAATCGCCGACGAGCCCGACGCCCTGCTGCAACAGCCGCCCGAACTCGCGaaa                   >  2:376139/1‑143 (MQ=255)
                                                                                                                     aGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGCAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc               >  1:736807/1‑143 (MQ=255)
                                                                                                                       aCATCGTACTGGACCAGCTGGGCGTCCCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc             <  2:420427/143‑1 (MQ=255)
                                                                                                                              aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCGGCAAGAAGTCGCGAAACTGCGCGAATTCa      <  1:158827/143‑1 (MQ=255)
                                                                                                                                  aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAACTCATACa  >  2:707813/1‑143 (MQ=255)
                                                                                                                                      |                                                                                                                                          
GGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184409‑4184681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑