Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA ← / → uspC	IS1 repressor TnpA/universal stress protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	41 (1.110)	30/284	0.2	100%	intergenic (‑271/‑267)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	62 (1.690)	39/284	0.0	100%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCG  >  NC_000913/2289972‑2290253                                                                                                                                              |                                                                                                                                             aaGAAAGTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATCTTAGGGCCTTGTTCGCACCTTCCa                                                                                                                                             >  1:44631/1‑143 (MQ=2)   gAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGCTGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACGTGATCTCGTATCAGGGACTTGTTCGCACCGTCCATa                                                                                                                                           >  2:26227/1‑143 (MQ=255)    aaacTCTCTCTTCTGTTCTGACGTCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATaa                                                                                                                                          <  1:343297/143‑1 (MQ=11)         ctctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCAAAACGCTg                                                                                                                                     >  2:424141/1‑143 (MQ=21)           cgtttCTGGTCGGTCGGCGCTTACTGGTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTa                                                                                                                                   <  2:621062/141‑1 (MQ=255)            ttttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAg                                                                                                                                  >  1:389749/1‑143 (MQ=38)               tCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTCGcca                                                                                                                               >  1:625749/1‑143 (MQ=38)                   gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAg                                                                                                                           <  1:406508/143‑1 (MQ=255)                     cTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGaa                                                                                                                         <  1:433690/143‑1 (MQ=255)                     cTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGaa                                                                                                                         >  1:649668/1‑143 (MQ=255)                       gACGTCGCTTACTCCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGACCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCATAACa                                                                                                                       <  2:333636/143‑1 (MQ=255)                       gACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGcaca                                                                                                                       >  2:525194/1‑143 (MQ=255)                       gACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACAAGAACa                                                                                                                       >  1:163740/1‑143 (MQ=255)                          ggCGCTTACTGCTGAATTCACGGTCGGCGAAGGTAAGTTGATGACTCATGATGATCCCTGTCCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGat                                                                                                                    <  2:584649/143‑1 (MQ=255)                             gcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGGTCGCACCGTCCATAACGCTGTAGCCACCAGAACAAGTAtt                                                                                                                 >  1:633905/1‑143 (MQ=255)                                   tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACCTGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCAACAGAACAGCTAATGCGGaa                                                                                                           >  2:451979/1‑143 (MQ=255)                                    gctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATCTCAGGGACTTGTTCGCCCTTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAAc                                                                                                          >  1:38099/1‑143 (MQ=255)                                          atCACAGTCGGCGAAGGTAAGATGCGGACTCATGATCAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACaaa                                                                                                    <  1:524481/142‑1 (MQ=18)                                                     cGAGGGTATGTTGATTACTCATGTTGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACGTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGaa                                                                                         <  2:536037/143‑1 (MQ=21)                                                     cGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAACCATGCTCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGaa                                                                                         >  1:605470/1‑143 (MQ=255)                                                     cGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGGTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGaa                                                                                         <  1:85410/143‑1 (MQ=255)                                                        aGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCa                                                                                      <  1:64126/143‑1 (MQ=255)                                                           tAACTTGAAGACTCAGGATGAACCGTGTTCTATGGCTCCAGATGACAAAGATGATCTCATATCAGGGACTTGTTCTCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGAt                                                                                   <  2:348682/143‑1 (MQ=25)                                                              gTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAGAGAGAAACAGAACCAGATTGa                                                                                >  1:37970/1‑143 (MQ=255)                                                                tgatgaCTCATGATGAACCCTGTTCTATGACCCCAGATTTGAAACATTATATCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATg                                                                              <  1:635999/143‑1 (MQ=25)                                                                ggatgaCTCGTGATGAACCCTGTTCTATCGCTCCAAATGACAAGTATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATg                                                                              <  1:572574/142‑1 (MQ=25)                                                                    gaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAtt                                                                          >  1:375517/1‑143 (MQ=255)                                                                       tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACACAGAGAAACAGAACCAGATTGATCCATTGac                                                                       >  1:58238/1‑142 (MQ=255)                                                                         atgatgAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACAAGAACAGATATTGCGGAACAACAACAAAAAAAAGAACCAGATTGATGCATTGAACt                                                                     >  2:173722/1‑143 (MQ=21)                                                                              gaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAtt                                                                          >  2:610088/1‑133 (MQ=255)                                                                              gaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAtt                                                                          <  1:610088/133‑1 (MQ=255)                                                                               agcccTGTTCTATGGCTCCAGATGCCAAACATGATCTCAGATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATc                                                               <  2:437481/141‑1 (MQ=255)                                                                                 cccTGTTCTGTGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAAAAACAGAACCAGATTGATGCACTGACCTTTCATCCt                                                             >  2:661735/1‑143 (MQ=255)                                                                                  ccTGTTCTATGGCTCCAGATGACAAACATGATGTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTa                                                            <  2:286279/143‑1 (MQ=255)                                                                                    tGTTCTATGGCTCCAGATGACAAACATGCTCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATg                                                          <  1:3016/143‑1 (MQ=255)                                                                                          aTGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGACGAACAAAACCAGAATGATGCATTGAGCTTTCATCCTATGAaatta                                                    >  2:560836/1‑143 (MQ=255)                                                                                            ggCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaattaat                                                  >  1:664354/1‑143 (MQ=255)                                                                                                 cAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGACCAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGCTGCCTTGAGCTTTCATCCTATGAAATTAATTGCTg                                             >  2:54876/1‑143 (MQ=255)                                                                                                       aCAACCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGAAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaaaa                                       <  1:474812/143‑1 (MQ=255)                                                                                                            cATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATCAAATTAATTGCTGTGAAAAGCAtt                                  >  2:610665/1‑143 (MQ=255)                                                                                                              tGATCTCACAGCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAtttg                                <  2:464711/143‑3 (MQ=255)                                                                                                               gATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGCACGACACAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATCGCTGTTAAAAGCATTggg                               >  1:151512/1‑143 (MQ=255)                                                                                                                   tcATATCAGGGACTTGTTCGCACGTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTCATGCATTGAGCCTTCATCCTATGAAGTTAATTGCTGTTAAAAGCGTTGGGTACa                           >  1:191543/1‑143 (MQ=255)                                                                                                                   tcATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATGGGGTACa                           >  1:218262/1‑143 (MQ=255)                                                                                                                    cATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAg                          <  2:561575/143‑1 (MQ=255)                                                                                                                              aCTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCa                >  1:200124/1‑143 (MQ=255)                                                                                                                                  gTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTCAAAAGCATTGGGTACAGAAAATACCGATAga            >  2:108085/1‑142 (MQ=255)                                                                                                                                      gCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAACCATAGCTGCa        >  2:280183/1‑143 (MQ=255)                                                                                                                                        aCCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCAtc      >  1:419256/1‑142 (MQ=255)                                                                                                                                            tCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCg  <  1:184945/143‑1 (MQ=255)                                                                                                                                              |                                                                                                                                             AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCG  >  NC_000913/2289972‑2290253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.