Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (‑2/+1)	glpR ← / ← glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	80.6 / NA	28	intergenic (‑2/+1)	glpR/glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (17/11);  total (17/11)

ATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGT  >  NC_000913/3560313‑3560583                                                                                                                                                |                                                                                                                                 atCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAGGGCAGCGCCGCACCGCCCTGCTGGc                                                                                                                                   >  1:205438/1‑143 (MQ=255)    gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgca                                                                                                                                <  1:569612/143‑4 (MQ=255)           aGCGTCGAGCCATTGGGGATTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCCTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAg                                                                                                                         <  1:165703/143‑1 (MQ=255)                    ccATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTc                                                                                                                >  1:15697/1‑143 (MQ=255)                    ccATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAACAGGTTTTGCTc                                                                                                                >  1:34309/1‑143 (MQ=255)                     cATTGGGGATTTGCTCCGCCACTTTGTTGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCGGCGCCGCACCCCCACGATCGCGCGGGACCAGGTTTTGCTcc                                                                                                               >  1:184574/1‑143 (MQ=255)                        tGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGACGGCAGCGCCGCACCGCCATGCTGGCGCAGGATCAGGCTTTGCTCCGcc                                                                                                            >  2:7858/1‑143 (MQ=255)                                tgttCCGCCACTTCGCGGGAGAGGCGCGCTTCGTCCTAGGGCAGGGGGGCCGTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                                                    <  1:112198/140‑1 (MQ=255)                                      gCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCCGGATCAGGTTTGGCTCCGCCAGCTCATTGAGGTc                                                                                              >  2:567001/1‑143 (MQ=255)                                      gCCACTTTGCGGGCGATGCGCTCTTTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                                                              <  2:377119/143‑1 (MQ=255)                                                       gcgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCg                                                                             <  1:67706/143‑1 (MQ=255)                                                          ctctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                          >  1:162279/1‑143 (MQ=255)                                                           tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGTCACGGCGTCTTAACCGAACTGGAAGGCAGCGCGGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTGCGCCCGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCt                                                                         >  2:446455/1‑143 (MQ=255)                                                             tttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATAGAGGTCGCGGCGAGTAGTCTGCGGGCTGa                                                                       >  2:136664/1‑143 (MQ=255)                                                                   ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgaga                                                                 <  1:271176/143‑1 (MQ=255)                                                                     cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAGTGAGGTCGCGGAGAATAGTCTGCGGGCTGACGGAGaaa                                                               >  2:558922/1‑143 (MQ=255)                                                                             gTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTAc                                                       >  2:464368/1‑143 (MQ=255)                                                                               ggCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCa                                                     >  2:352247/1‑143 (MQ=255)                                                                                       gATCGTGCTACGGCGTGTTAACCGAACAGGAAGGCAGTGCCGCGCCGCCATGATGGCGCAGGATCAGGGTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                             <  2:183749/143‑1 (MQ=255)                                                                                         tCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                           >  1:537797/1‑143 (MQ=255)                                                                                           gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGCTGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGAACGCAACAAATAGTCTGCGGGCTGACGGAGAAATGCAATACGAGCTCTTCGGTAc                                         >  1:606281/1‑143 (MQ=255)                                                                                            tGCCACGGCGTGTTAACCGACCTGGCAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACt                                        <  1:99096/143‑1 (MQ=255)                                                                                            tGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACAGCCAAGCAGGCGCAGCATCAAGTTTTTCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACt                                        <  1:281959/143‑1 (MQ=255)                                                                                               cACGGCGTGTTAACCGAACTGGACGGCAGCGACGCACCGTCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGAc                                     <  2:567592/143‑1 (MQ=255)                                                                                                     gtgtTAACCGAACTGGAAGGCAACGACGCACAGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTGATAGAGGTGGCGGCGAATAGTCTGCGGGCGGACGGAGAAATGCTCTACGAGCTCTGCGGTACTGACATAAcg                               >  2:201795/1‑142 (MQ=255)                                                                                                              gAACTGGAAGGCAGCGCCGCGCCGCCATGATGGCGCAGGATCAGGTTGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt                      <  2:273007/143‑1 (MQ=255)                                                                                                                  tGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCACATTGAGCACGCGGCGAAGAGTCTGCGGGCTGACGGAGAAATGCGCGACCAGCGCGTCGGTACTGACATAAACCAGCTGGATAAcc                  >  2:64566/1‑143 (MQ=255)                                                                                                                          aGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCCCGCCGAATACTCTCCAGGCAGACGGAGAAATGCTCTACAAGCACTTCAGTACTAAAATAACCATGATGTTTAACAAGTTCCat          >  1:496063/1‑143 (MQ=255)                                                                                                                               cgccgctccATGATGGCGCAGGCTCAGGTTTTGCTCCGCCAGCGCATTGAGGTCGCGCCGAATAGTCTGCGGGCTGACAGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc     <  2:340007/136‑1 (MQ=255)                                                                                                                                  aCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGt  >  1:334527/1‑143 (MQ=255)                                                                                                                                                |                                                                                                                                 ATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGT  >  NC_000913/3560313‑3560583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑