| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,173,361 | 0 | C | . | 96.4% | 107.2 / ‑0.3 | 29 | pseudogene (2/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base . (14/13); minor base T (1/0); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01 | |||||||||||
| * | NC_000913 | 2,173,362 | 0 | C | . | 96.3% | 100.8 / 0.8 | 29 | pseudogene (1/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base . (14/12); minor base A (1/0); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGT > NC_000913/2173223‑2173496 || aaccACGAGGGACGGGGACCGCCAGCGCCACGAAGAAGCGGATGGGGGCAAGAGCGCCAAGCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgcc < 1:42893/141‑4 (MQ=255) ccACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccgt < 1:527301/143‑6 (MQ=255) cccGGCGACCGCCATCGCCACGAAGACGCCGTCGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCca < 2:94055/141‑1 (MQ=255) ggagACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagca < 1:212150/140‑1 (MQ=255) tCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGCTTACCCGGCACACCAACAGCAATTAAAACGGTGAGTGGGCTAAAAATCAGGCTTGCCGGTACCA‑‑CCGCCGTACGCCCCAGCAGCAACGcccaa > 2:285156/1‑140 (MQ=255) tCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAACCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGa > 2:618072/1‑143 (MQ=255) aCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGACGTATGTCCCAGCAGCAACGCCGGATCAAg > 1:384462/1‑143 (MQ=255) aCGAAGAACCCGCTGGTGCCAAGATCGCCAAACGGCAGCACCTCATTACCCGGCACACAAACAGCAATTCAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAg < 1:134932/143‑1 (MQ=255) gCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCa < 1:80476/143‑1 (MQ=255) agggtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCACTTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGACCAAGGCCAATCAgcac > 2:146224/3‑140 (MQ=255) ggCAAGATCGCCAAACGGCAGCACCTGACTACCCCGCAAAAAGACAGCCATTTAAGTGGCGTGTGGGATCAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTACCTGCAGTAACGCCGGATCAAGGCCAATCAGGAACTCCt < 2:171192/143‑1 (MQ=255) gCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAGAAATCAGGCTTCGCGATACCA‑‑CCGCCGTAGGTCCCAGCAGCAACGCCGGAGCAAGGCCAATCAGGGACGCGTGAccgccgc > 2:468979/1‑142 (MQ=255) ccAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATAGCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCACGGCCAATCAGGGACTCCTGAcagcacga > 1:269491/1‑139 (MQ=255) cAAACGGCAGCACCTGATTACCCGGCGCACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAAc < 1:470213/143‑1 (MQ=255) ggCAGCACCTGATTACCCGGCACACAAACATAAATGAATATGGTGAGCGGGAGCTACAACAGGAGGGCACGT‑CCATACTG‑GTTATGTGCCAGCAGGAGCGCCGGATCAAGGCCCATCGGGAACTCCTGACCGCCGAACGTCGc > 2:657387/1‑143 (MQ=255) cagcaCCTGATTGCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCt < 1:511765/143‑1 (MQ=255) cccGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGCCTaag > 1:485234/1‑141 (MQ=255) cGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAg > 1:355790/1‑143 (MQ=255) ggCACACAAACAGCAATTAAAATGGTGAGTGAGATAAAAATCAGGCTTGCCGATACCA‑‑CAGACGTATGTCACAGCAGCAACGCCGGATCAAGGACCATCCGGAACTCCTGACCGCCGAACTTCGCCCGTAAACGACtctaagc > 2:468835/1‑137 (MQ=255) gCACACAAACAGCAATTAAAATGGGGAGTGGGATAAAAATCATGCTTGCCGATACCA‑‑CCGCCGTATGTCCCCCCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGcc < 1:164846/143‑1 (MQ=255) ctacagtGGGGAGTGGGATAGAAATCGGGCTGGCCGGTACCA‑‑CCGTCGTATGTCGCAGCAGCAGCGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGc < 2:533516/137‑1 (MQ=255) aaaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGACTGTAAACGACTACGAGCCCGCTTAGCGATGGGCgct > 2:421324/1‑141 (MQ=255) aGTGGGATAAAAATCAGGCTTGCCGATA‑CA‑GGCGCGGTAGGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatc < 2:295452/143‑1 (MQ=255) ggtAAACATCAGGCTGCCCGATCCCA‑‑CCGCCGTGTGTCCCAGCAGCAGCGCCGGATCAAGGCCAATCACCAGCGGCTGACCGCCGAAGTTGGCCTGTAAACGACGACGAGCCTGCGTAGCGATGGGCGTTAAACCATCCatga < 2:463662/141‑1 (MQ=255) gacaagaaTCATTCTTCCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCCACGGCGGATCAAGGCCAATCAGGCACTCCTGTCCGCCGAACTTCGCCTGTAAACGACTACTAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga < 2:205090/137‑1 (MQ=255) aTAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCCCCGACCTTCGCCTGTCAACCACTACGAGCCTGCTTACCGATGCGCCTCAAACCCTCcatcat > 2:87542/1‑143 (MQ=255) aaaaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAGCGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGCGCCTGCTTAGCGATGGGCGTTACAGCATCCACGATgg > 2:204349/1‑143 (MQ=255) agaagcAGGCTCGCTGATACCA‑‑CCGCCGGATGTCCCAGCACTAACGCCGGATCAAGGCGAATCAGGAACTCCTGACCGCCGAATTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATggg < 1:239267/138‑1 (MQ=255) aaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGt > 2:447214/1‑143 (MQ=255) tCAGGCTTGCCGGTACCA‑‑CCGCCGGATGTCCCAGCAGCGACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTa < 2:313035/143‑1 (MQ=255) ccGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGGCTGCTTAGCGATGGGCGTTCAACCATCCATGATGGGTTTCATCACCCGt > 1:658640/1‑143 (MQ=255) || TTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGT > NC_000913/2173223‑2173496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |