Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA ← / → uspC	IS1 repressor TnpA/universal stress protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	63 (1.640)	47/284	0.0	100%	intergenic (‑271/‑267)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	55 (1.430)	33/284	0.2	100%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGA  >  NC_000913/2289972‑2290255                                                                                                                                              |                                                                                                                                               aagaccaTCTCTTGTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATTACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCa                                                                                                                                               <  1:195622/137‑1 (MQ=2) aaGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCa                                                                                                                                               >  1:208608/1‑143 (MQ=11)      aaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTACGTTGCTGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATagtt                                                                                                                                          >  1:380810/1‑140 (MQ=2)          tctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTga                                                                                                                                      >  2:78395/1‑142 (MQ=25)            ttttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAGGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACACTGTAg                                                                                                                                    >  2:317244/1‑143 (MQ=25)               tCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGcca                                                                                                                                 >  1:4482/1‑143 (MQ=40)                  ggTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccacca                                                                                                                              <  2:324031/143‑1 (MQ=255)                              ctcaCTGCTGAATGCACTGCCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATACCGCTGTAGCCACCAGAACAGATATTg                                                                                                                  <  1:38375/140‑1 (MQ=255)                              cTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTg                                                                                                                  <  1:164751/143‑1 (MQ=255)                                   tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTGCAGATGACCAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGaa                                                                                                             >  1:399673/1‑143 (MQ=255)                                   tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCCGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCCGAACGGATATCGCGGaa                                                                                                             >  2:160050/1‑143 (MQ=255)                                    gctgAATTCTCTGTCGGCGAAGGTAAGTTGATGACTCATTATGACCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACTGATATTGCGGAAc                                                                                                            <  1:361078/143‑1 (MQ=255)                                       gtaTTCACTGTCGGCGCACGTTAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACTCACATGATCTCGTAGCAGCGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGAc                                                                                                         <  2:15703/141‑1 (MQ=18)                                                gTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCGTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGaaa                                                                                                <  2:610333/143‑1 (MQ=255)                                                 tCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAAc                                                                                               <  1:388057/143‑1 (MQ=255)                                                         ggTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAg                                                                                       <  1:532174/143‑1 (MQ=255)                                                              gTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGa                                                                                  >  1:597475/1‑143 (MQ=255)                                                                tgatgaCTCATGATCAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATg                                                                                <  1:254248/143‑1 (MQ=255)                                                                   tgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAt                                                                             <  1:313450/143‑1 (MQ=255)                                                                    gaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAtt                                                                            >  1:642667/1‑143 (MQ=255)                                                                       tCATGATGAACCCTGTACTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAAAGTTATAGCCACCAGAACAGATATTACGAAACGACAACGAGAAACAGAACCAGATTGATGCATTGaa                                                                         >  2:24456/1‑142 (MQ=21)                                                                           gatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCttt                                                                     >  2:129781/1‑143 (MQ=255)                                                                            atgcACCCGGTTGTGTGGCTCCAGATGACGCACATCATCCAACATCAGCGGGAGGTTCGCACCGTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGTTGCATTGAGCTTTc                                                                    <  2:455782/143‑1 (MQ=255)                                                                              gaACCCTGTGCTATGGCTCCAGATCACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCAt                                                                  <  1:682480/143‑1 (MQ=255)                                                                              gaACCCCGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCAt                                                                  <  1:73531/143‑1 (MQ=255)                                                                               aaCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATAAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACACATATTGCGGAACGCCAACGAGAAACAGCAACAGATACCTGCATTGACCTTTCATc                                                                 >  1:307239/1‑143 (MQ=21)                                                                                 cccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCt                                                               >  1:252600/1‑143 (MQ=255)                                                                                   cTGTCCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTAt                                                             <  2:694474/143‑1 (MQ=255)                                                                                    tGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATg                                                            >  1:443037/1‑143 (MQ=255)                                                                                    tGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTACGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACACAGAGAACCAGAACCAGATTGACGCATCGACCTTTCATCATATg                                                            >  2:275498/1‑143 (MQ=25)                                                                                      ttCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGaa                                                          >  1:304397/1‑143 (MQ=255)                                                                                           tGGCTGCAGATGGCAACCATGATATCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGCaattaa                                                     <  2:606440/143‑1 (MQ=38)                                                                                           tGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATAGTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaattaa                                                     <  1:23853/143‑1 (MQ=255)                                                                                             gCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGCACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaagtaatt                                                   >  2:72297/1‑143 (MQ=255)                                                                                              cTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACACAGAGAAACAGAACCAGCTTGATGCATTGAGCCTTCATCCTATGCAACTAATTg                                                  >  2:152478/1‑143 (MQ=38)                                                                                               tCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGc                                                 >  2:438275/1‑143 (MQ=255)                                                                                                      gACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaaa                                          >  2:591371/1‑143 (MQ=255)                                                                                                       aCAAACATGATCTCATATCAGGGACTTGTGCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaaaa                                         <  1:191519/143‑1 (MQ=255)                                                                                                        cAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAg                                        <  1:372497/143‑1 (MQ=255)                                                                                                         aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGc                                       >  1:343967/1‑143 (MQ=255)                                                                                                            cATGATCTCATATCAGGGACTTGTTCGCAGCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAtt                                    <  1:383432/143‑1 (MQ=255)                                                                                                               gATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTggg                                 >  1:265245/1‑143 (MQ=255)                                                                                                                  ctcATATCAGGGACTTGTTCGCACCTCCCCTAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTAc                              <  1:240572/143‑1 (MQ=255)                                                                                                                   tcATATCAGGGACTTGTTCGCACCTTCCATAACGCTATAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACAAGATTGATGCATTGAGCATTCATGCTATAAAATGAATTGCTGTTGAAAGCAtttaggact                             >  2:430478/1‑136 (MQ=255)                                                                                                                        gcAGGGGCTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACTAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGaaaa                        <  2:235291/142‑1 (MQ=255)                                                                                                                            ggACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATGAATTGCTGTTAAAAGCATTGAGTACAGAAAATaac                    >  2:374505/1‑141 (MQ=255)                                                                                                                            cgtcttcttCGCACCTTCCATAACGCTGTCGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAcc                    <  1:137916/140‑1 (MQ=255)                                                                                                                                ttGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACGAAGAGAAACAGAAACAGATAGAGGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCGTTGGATACAGAAAATAcaacta                >  2:421473/1‑138 (MQ=255)                                                                                                                                      gCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAAAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGGTAAAAGCATTGGGTACAGAAAATACGCAAAGCTCCa          >  2:363728/1‑143 (MQ=255)                                                                                                                                         ccTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATAc       >  1:446335/1‑143 (MQ=255)                                                                                                                                         ccTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCAATAc       >  1:635963/1‑143 (MQ=255)                                                                                                                                              cATAACGCTGTAGCCACCAGTACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGa  <  1:534869/143‑1 (MQ=255)                                                                                                                                              cATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGa  <  1:323436/143‑1 (MQ=255)                                                                                                                                              cATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGGTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGa  >  2:61761/1‑143 (MQ=255)                                                                                                                                              |                                                                                                                                               AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGA  >  NC_000913/2289972‑2290255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.