Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 759,912 C→A R403S (CGT→AGT)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913759,9120CA96.5% 161.7 / NA 58R403S (CGT→AGT) sucA2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base A (28/27);  minor base G (1/1);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTCCGTAACACCTTTAAACGTGAT  >  NC_000913/759773‑760046
                                                                                                                                           |                                                                                                                                      
tGACGCCGCAGTGACCGGGCAGGGCGTGGTTCGGGGGACCCGGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGtt                                                                                                                                     <  1:1243595/143‑1 (MQ=255)
    gccgcAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACCACCAGGTTGGTTTCACCACCTCTGATCCGCCGGCCGCCCGTTCCAc                                                                                                                                 >  2:143215/1‑143 (MQ=255)
      cgcAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGc                                                                                                                               >  1:161649/1‑143 (MQ=255)
         aGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCgt                                                                                                                            >  2:107755/1‑143 (MQ=255)
          ttgtccgggccgggCGTGGTTCAGGCAACGCTGGACATGTCGAAAGCGCGTGGTTATGATGTTGGCGGTACGGTACGTATCGTTCTCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCgta                                                                                                                           <  2:652009/139‑1 (MQ=255)
                 ggCTGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCgtactgtact                                                                                                                    <  2:183356/143‑1 (MQ=255)
                      ggCGTGGTTCAGGACACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTAGCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGatat                                                                                                               <  2:733866/143‑1 (MQ=255)
                        cGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCg                                                                                                             >  2:1157550/1‑143 (MQ=255)
                             ttCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTCCGTATCGTTGTCAATATCCAGGTTGGCTTCACCACCTCTAATCCGCTGGATGCCACCTCTCCGCCGTACTGTACTGATATCGGTCAg                                                                                                        >  2:156536/1‑143 (MQ=255)
                             ttCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACACCCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTCGTACGCCGTACTGTCTTGGTCTCGGCAAg                                                                                                        >  2:912015/1‑143 (MQ=255)
                             ttCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAg                                                                                                        >  1:162347/1‑143 (MQ=255)
                               cAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCGGTTCAACGCCGTACTGTACTCATATCGGTaacgc                                                                                                      >  2:334234/1‑140 (MQ=255)
                                aGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCATGTTTGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATg                                                                                                     <  1:944528/143‑1 (MQ=255)
                                aGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATg                                                                                                     >  1:335698/1‑143 (MQ=255)
                                       ccTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAgg                                                                                              >  1:927106/1‑143 (MQ=255)
                                          gAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATGTCGGTAAGATGGTTCAGGccc                                                                                           >  1:642905/1‑143 (MQ=255)
                                          gAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGccc                                                                                           <  2:485994/143‑1 (MQ=255)
                                          gAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACGGTACTGATATCGGTAAGAAGGTTCAGGccc                                                                                           >  1:562873/1‑143 (MQ=255)
                                                    aaggtgcgTGGTTATGAATTGGTCGGTACGGTACGTATCGGTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCa                                                                                 <  1:391666/138‑1 (MQ=255)
                                                    aaggcgcGTGTTTATGCACTTGGCGGTACGGTACGTATTGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCa                                                                                 <  2:1044269/140‑1 (MQ=255)
                                                          cgTGGTTATGAAGGTGGCGGTACGGTACGTACCGTTATCAACAACCAGGTTGGTTTCACGACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTaa                                                                           <  1:133328/143‑1 (MQ=255)
                                                               gtctGAAGATGGCGTTACGGTACGTATCGGTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCgg                                                                      <  2:619580/140‑1 (MQ=255)
                                                                tATGGAGTGCGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGa                                                                     <  2:1020488/143‑1 (MQ=255)
                                                                 aTGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGCTGCCAGTTCTACGCGGTACTGTACTGATATCGGTAAGATGGTTGAGGCCCCGCTTTTCCACGTTAACGAGGAc                                                                    >  2:1128515/1‑143 (MQ=255)
                                                                  tGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACg                                                                   <  1:965456/143‑1 (MQ=255)
                                                                   gAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAGCGCGGGCGa                                                                  >  1:84409/1‑143 (MQ=255)
                                                                   gAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGAGTTTCCACGTTCACGCGGACgc                                                                  >  1:177753/1‑142 (MQ=255)
                                                                       ttGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCGAATCCGCTGGATGGCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCg                                                              >  1:830132/1‑143 (MQ=255)
                                                                            ggtactgtacGTAGCGTGATCAACCACCAGGTTGGTTTCACCACCTCTGATCCGCTGGTTGCCGGTTCTACGCCGTTCTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTTACGCGGACGATCCGGAAGc                                                         <  2:659009/143‑1 (MQ=255)
                                                                                cggtccgTATCGTTATCAACAACCAGGTTGGTTCCACCACCTCTAAGCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGtt                                                     <  2:377766/143‑1 (MQ=255)
                                                                                cggtacgTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGtt                                                     <  1:149398/143‑1 (MQ=255)
                                                                                  gtacgTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGc                                                   >  2:1029309/1‑143 (MQ=255)
                                                                                   tacgTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTACCACGTTAACGCGGACGATCCGGAAGCCGTTGcc                                                  >  1:1244179/1‑143 (MQ=255)
                                                                                      gTATCGTTATCAACAGCGAGGTTGGTTTCACCACTTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCTCGTTAACGCGGACGATCCGGAAGCCGTTGCCttt                                               <  2:37898/143‑1 (MQ=255)
                                                                                       tATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTtg                                              <  2:161649/143‑1 (MQ=255)
                                                                                        agcGTTATGAACCACCAGGTTGGTTTCATCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTtgt                                             <  2:40483/141‑1 (MQ=255)
                                                                                        aTCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTtgt                                             >  1:1051044/1‑143 (MQ=255)
                                                                                         tCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGCCGATCGGGAAGCCGTTGCTCTtgtg                                            >  2:572452/1‑143 (MQ=255)
                                                                                          cGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGa                                           <  2:850118/143‑1 (MQ=255)
                                                                                           gTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGGTCCGGAAGCCGTTGGCtgtgtggc                                          >  1:477518/1‑141 (MQ=255)
                                                                                           attATCCACGACCTGGTGGTGTTCACCACGTCTGATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGAc                                          <  2:1055148/142‑1 (MQ=255)
                                                                                            tgaTCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGAcc                                         <  1:1079775/141‑1 (MQ=255)
                                                                                                 aacaacCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCt                                    >  2:441532/1‑143 (MQ=255)
                                                                                                   caacCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTgg                                  <  1:616521/143‑1 (MQ=255)
                                                                                                    aacCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGACGCCGTTGCCTTTGTGACCCGTCTgcc                                 >  1:798765/1‑141 (MQ=255)
                                                                                                      ccAGGCTGGTTTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGCACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGgcgc                               <  1:146982/143‑1 (MQ=255)
                                                                                                              gttTCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCCCGCTTTc                       >  2:473097/1‑143 (MQ=255)
                                                                                                               tttcttcaccTCTAATCCGCTGGACGCGAATTCTACGCCGGACCGTACAGATAGCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTcc                      <  1:1017281/137‑1 (MQ=255)
                                                                                                               tttCCCCACCTTTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTcc                      <  2:830132/143‑1 (MQ=255)
                                                                                                               tttCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGCTGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCATCTGTGCCCCGTCTGGCGCACGGTTTcc                      >  2:1006656/1‑143 (MQ=255)
                                                                                                               tggcaccgcCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCGGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTcc                      <  1:1254942/140‑1 (MQ=255)
                                                                                                                 tCACCACCTCTAATCCGCTGGATGCCAGTTCTACGCCGTACCGTACTGCGGTCGGTAAGGTGGTTCAGGCCCCGATTTTCCCCGTTAACGCGGTCGTGCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTCCGt                    <  1:1176107/143‑1 (MQ=255)
                                                                                                                          ctAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCCGAAACCGTTGCCTTTATGACCCGACTGGCGCTCGATTTCCGTAACACCtat           >  1:303432/1‑141 (MQ=255)
                                                                                                                           tAATCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGGAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCCCGAGTTCCGTAACACCTTTa          >  2:567592/1‑143 (MQ=255)
                                                                                                                            aaTCCGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTCCGTAACACCTTTaa         >  1:1124812/1‑143 (MQ=255)
                                                                                                                                cGCTGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGCTCCGGAAGCCGTTGCCTTTGTGACCAGTCTGGCGCTCGATTTCCGCACCACCTTTAAGCgg     >  2:1095561/1‑142 (MQ=255)
                                                                                                                                  atGGATGCCAGTTCAACGCCGGACGGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTCCGTAACACCTTTAAACGTGa   <  1:107755/142‑1 (MQ=255)
                                                                                                                                   tGGATGCCAGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTCCGTACCACCTTTAAACGTGAt  >  1:10595/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                      
TGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTCCGTAACACCTTTAAACGTGAT  >  NC_000913/759773‑760046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑